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CTRI Number  CTRI/2024/07/070092 [Registered on: 05/07/2024] Trial Registered Prospectively
Last Modified On: 03/07/2024
Post Graduate Thesis  Yes 
Type of Trial  Interventional 
Type of Study   Other (Specify) [NEBULIZED COLISTIN IN PATIENT WITH VAP - CRAB]  
Study Design  Randomized, Parallel Group Trial 
Public Title of Study   Comparison of antibiotics given through vein only versus antibiotics given through vein and nebulization for treating patients who develop pneumonia while being on ventilator 
Scientific Title of Study   Aerosolized Colistin in Ventilator Associated Pneumonia caused by Carbapenem Resistant Acinetobacter 
Trial Acronym  nil 
Secondary IDs if Any  
Secondary ID  Identifier 
NIL  NIL 
 
Details of Principal Investigator or overall Trial Coordinator (multi-center study)  
Name  Ashwini A Kundar 
Designation  Senior Resident 
Affiliation  Aiims 
Address  Room no 5011,Anesthesiology office,Department of Anesthesiology, Pain medicine and Critical Care,AIIMS,Sri Aurobindo Marg,Ansari Nagar East

New Delhi
DELHI
110029
India 
Phone  9611942622  
Fax    
Email  ashwinikundar3@gmail.com  
 
Details of Contact Person
Scientific Query
 
Name  Dr Souvik Maitra 
Designation  Additional Professor 
Affiliation  Aiims 
Address  Room no 5011,Anesthesiology office,Department of Anesthesiology, Pain medicine and Critical Care,AIIMS,Sri Aurobindo Marg,Ansari Nagar East

New Delhi
DELHI
110029
India 
Phone  8146727891  
Fax    
Email  souvikmaitra@live.com  
 
Details of Contact Person
Public Query
 
Name  Ashwini A Kundar 
Designation  Senior Resident 
Affiliation  Aiims 
Address  Room no 5011,Anesthesiology office,Department of Anesthesiology, Pain medicine and Critical Care,AIIMS,Sri Aurobindo Marg,Ansari Nagar East

New Delhi
DELHI
110029
India 
Phone  9611942622  
Fax    
Email  ashwinikundar3@gmail.com  
 
Source of Monetary or Material Support  
Room no 5011,Anesthesiology office,Department of Anesthesiology, Pain medicine and Critical Care,AIIMS,Sri Aurobindo Marg,Ansari Nagar East Pin code - 110029 New Delhi India 
 
Primary Sponsor  
Name  All India Institute Of Medical SciencesNew Delhi 
Address  Room no 5011,Anesthesiology office,Department of Anesthesiology, Pain medicine and Critical Care,AIIMS,Sri Aurobindo Marg,Ansari Nagar East Pin code - 110029 New Delhi India 
Type of Sponsor  Research institution and hospital 
 
Details of Secondary Sponsor  
Name  Address 
NIL  NIL 
 
Countries of Recruitment     India  
Sites of Study  
No of Sites = 1  
Name of Principal Investigator  Name of Site  Site Address  Phone/Fax/Email 
Dr Ashwini A Kundar  All India Institute Of Medical Sciences  Room no 5011,Anesthesiology office,Department of Anesthesiology, Pain medicine and Critical Care,AIIMS,Sri Aurobindo Marg,Ansari Nagar East
New Delhi
DELHI 
9611942622

ashwinikundar3@gmail.com 
 
Details of Ethics Committee  
No of Ethics Committees= 1  
Name of Committee  Approval Status 
Institute Ethics Committee For Post graduate Research, All India Institute Of Medical Sciences  Approved 
 
Regulatory Clearance Status from DCGI  
Status 
Not Applicable 
 
Health Condition / Problems Studied  
Health Type  Condition 
Patients  (1) ICD-10 Condition: J168||Pneumonia due to other specified infectious organisms,  
 
Intervention / Comparator Agent  
Type  Name  Details 
Intervention  Aerosolized Colistin  Dose : 4 MU (diluted in 4ml sterile normal saline 0.9%). Route of administration- Nebulization. Nebulization will be given via an ultrasonic nebulizer . Each nebulization will take around 5-10 minutes. Frequency - two times per 24 h. Duration of therapy - 14 days 
Comparator Agent  Intravenous Polymyxin   Dose - 15 lakh unit loading dose intravenous followed by 7.5 lakh units BD. Route of administration - Intravenous. Frequency - Twice a day. Duration of therapy - 14 days.  
 
Inclusion Criteria  
Age From  18.00 Year(s)
Age To  99.00 Year(s)
Gender  Both 
Details  1)Hospitalized adults, aged ≥ 18 years
2)Microbiologically documented VAP due to A. baumannii with carbapenem resistant strains but susceptible to Polymyxin.

 
 
ExclusionCriteria 
Details  1)Patients’ refusal
2)Known allergies or sensitivities to nebulized colistin.
3)Pregnancy
4)Patients not expected to survive beyond 48h.
 
 
Method of Generating Random Sequence   Computer generated randomization 
Method of Concealment   Alternation 
Blinding/Masking   Participant Blinded 
Primary Outcome  
Outcome  TimePoints 
The primary outcome of this study is to evaluate the clinical response in two groups after 14 days of antibiotic therapy (IV alone versus IV and aerosolized antibiotics )for Carbapenem Resistant Acinetobacter baumanii Ventilator Associated Pneumonia.  Day 14 from start of antibiotic regimen 
 
Secondary Outcome  
Outcome  TimePoints 
1)Microbiological outcome at day 7 & day 14
2)Liberation from MV at day 28
3)ICU mortality
4)Hospital mortality
5)28- day mortality
6)Incidence of new onset or worsening AKI
7)Incidence of bronchospasm during nebulization
 
Day 7 & Day 14 from start of antibiotic regimen 
 
Target Sample Size   Total Sample Size="229"
Sample Size from India="229" 
Final Enrollment numbers achieved (Total)= "Applicable only for Completed/Terminated trials"
Final Enrollment numbers achieved (India)="Applicable only for Completed/Terminated trials" 
Phase of Trial   Phase 4 
Date of First Enrollment (India)   14/07/2024 
Date of Study Completion (India) Applicable only for Completed/Terminated trials 
Date of First Enrollment (Global)  Date Missing 
Date of Study Completion (Global) Applicable only for Completed/Terminated trials 
Estimated Duration of Trial   Years="2"
Months="6"
Days="0" 
Recruitment Status of Trial (Global)   Not Applicable 
Recruitment Status of Trial (India)  Not Yet Recruiting 
Publication Details   N/A 
Individual Participant Data (IPD) Sharing Statement

Will individual participant data (IPD) be shared publicly (including data dictionaries)?  

Response - YES
  1. What data in particular will be shared?
    Response - All of the individual participant data collected during the trial, after de-identification.

  2. What additional supporting information will be shared?
    Response -  Study Protocol
    Response -  Statistical Analysis Plan
    Response - Informed Consent Form
    Response - Clinical Study Report
    Response -  Analytic Code

  3. Who will be able to view these files?
    Response - Anyone

  4. For what types of analyses will this data be available?
    Response - Any purpose.

  5. By what mechanism will data be made available?
    Response - Proposals should be directed to [ashwinikundar3@gmail.com].

  6. For how long will this data be available start date provided 01-06-2026 and end date provided 01-06-2031?
    Response - Beginning 3 months and ending 5 years following article publication.

  7. Any URL or additional information regarding plan/policy for sharing IPD? 
    Additional Information - nil
Brief Summary  

                              Background / Purpose of trial

Ventilator-associated pneumonia (VAP) is a serious and common complication for patients in  intensive care unit  with considerable morbidity and mortality. Carbapenem resistance in Acinetobacter baumannii (CRAB) infection has become increasingly prevalent worldwide and has been associated with high mortality rate. Patients with ventilator-associated pneumonia (VAP) caused by carbapenem-resistant Acinetobacter baumanii (CRAB) ,  incidence of which has been rising substantially, may be predisposed to poor outcome because of limited therapeutic options. Polymyxins are one of the few agents that can be used for treatment of CRAB infections, and there is considerable evidence regarding their efficacy   for VAP caused by CRAB.

Nebulized antibiotics have been used to treat respiratory tract infections over  last 70 yearsThere has been a recent resurgence in interest for this administration route because of emergence of multidrug-resistant (MDR) strains as causative pathogens of severe respiratory tract infections. Many theoretical advantages of nebulized antibiotics have been proposed, such as higher concentrations at the site of infection and less systemic exposure.Several theoretical reasons for using nebulized antimicrobial therapy in mechanically ventilated patients have been postulated. With proper drug delivery, drug is delivered directly to  site of infection so that concentrations in the lung are high and  systemic toxicity is minimized.

Furthermore,  microflora of the gut is not altered, thus reducing  emergence of MDRO and infection with Clostridium difficile. The high antibiotic concentrations achieved with targeted therapy far exceed  MIC and result in a large ratio of maximum concentration to MIC, an index shown to be important for eradication of these organisms in milieu of thick purulent secretions, biofilm, and diminished mucociliary clearance.

 Increasing prevalence of VAP caused by highly resistant P aeruginosa and Acinetobacter has led to reintroduction of colistin (polymyxin E) in an aerosolized form and  IV form.The mechanism of colistin’s bactericidal activity is destabilization of lipopolysaccharide (LPS) of the outer membrane, and, in addition, it neutralizes LPS, thereby decreasing antiendotoxin activitites.

Depending upon type of antibiotics used, antibiotics administered via systemic administration may not penetrate  parenchymal lung tissue and bronchial secretions, resulting in insufficient drug concentration at target site. Moreover, altered antibiotic pharmacokinetics in critically ill patients has been recognized as an important factor that compromises optimal drug penetration. Inadequate concentration of antibiotics at infection site may result in poor treatment outcomes, particularly when MDR pathogens are etiology. Therefore, there is a requirement for drugs that can demonstrate the achievement of high concentrations at the site of infection, while also reducing risk of systemic toxicity caused by intravenously administered antibiotics. Accordingly, aerosolized antibiotics have been used as a rescue or adjuvant therapy in patients who do not exhibit responses to systemic treatment alone.

As per the recent HAP/VAP guidelines (2016) of the Infectious Diseases Society of America (IDSA) and American Thoracic Society (ATS), adjunctive therapy with aerosolized antibiotics is recommended along with administration of systemic antibiotics rather than systemic antibiotics alone for patients with gram-negative VAP (not HAP) ‘only’ susceptible to aminoglycosides or polymyxins (Colistin/Polymyxin B).

Hence we will be conducting this study where we will administer aerozolized colistin in carbapenem resistant acinetobacter patients and see the outcome

 

AIMS AND OBJECTIVE

The study aims to investigate  outcomes of two different treatment approaches for Intensive Care Unit patients with Ventilator-Associated Pneumonia (VAP) caused by Carbapenem Resistant Acinetobacter baumannii. The two treatment approaches being compared are:

Intravenously Administered Polymyxins alone

Combination Therapy - IV Polymyxin along with Aerosolized Colistin Nebulization

Methodology

 Study design

 Randomized. Double blind placebo controlled trial

 Study setting

Various intensive care units of Department of Anaesthesiology, Pain Medicine & Critical Care

 

Inclusion criteria:

1)    Hospitalized adults, aged ≥ 18 years

      2)    Microbiologically documented VAP due to A. baumannii with carbapenem resistant

             strains but susceptible to Polymyxin.

 

Exclusion criteria :

Patients’ refusal

Known allergies or sensitivities to nebulized colistin.

Pregnancy

Patients not expected to survive beyond 48h.

Study Protocol:

All patients satisfying the inclusion criteria will be included in the study after obtaining written informed  consent from the patients’ legally acceptable representative

 Definition of Ventilator Associated Pneumonia :

 A pneumonia where the patient is on mechanical ventilation for > 2 consecutive calendar days on the date of event, with day of ventilator placement being Day 1

Pneumonia will be  identified by using a combination of imaging, clinical, and laboratory criteria.

Microbiological diagnosis of VAP will be established by positive cultures of endotracheal aspirate or bronchoalveolar lavage with isolation of  A. baumannii with carbapenem resistant strains but susceptible to Polymyxin .  Baseline demographic data and severity of illness (Acute Physiology and Chronic Health Evaluation (APACHE II)) , and Sequential Organ Failure Assessment(SOFA) scores , septic shock  and acute renal failure (defined by KDIGO -The Kidney Disease: Improving Global Outcomes definition)comorbidities, colistin regimen (intravenous versus intravenous and inhalatory) and length of treatment will be recorded. Bacteriologic sampling will be  performed for all patients on the day that VAP is suspected (day 0), before new antimicrobials are started. The response to treatment will be  assessed at the time of discharge from the ICU or at the end of antimicrobial therapy, especially if the patient remained hospitalized for a non VAP-related disease.

Included patients will be randomized into two parallel groups whether they will receive combination therapy of intravenous Polymyxins and aerosolized colistin or IV Polymyxins alone.

•       IV Polymyxin plus sulbactam or IV Polymyxin plus minocycline

•        Aerosolized colistin will be given as 4 MU (diluted in 4ml sterile normal saline 0.9%) by nebulization two times per 24 h.

•       Nebulization will be given via an ultrasonic nebulizer.

•       The treatment duration will be maintained at least 14 days or till extubation.

•       All patients will be followed till 28days or death or hospital discharge.

 

 

Primary Outcome

 Primary outcome will be clinical outcome of VAP assessed at day 14 of therapy and  classified as

Clinical Cure 

Clinical Improvement 

Clinical Failure 

Clinical Success 

Secondary Outcomes

Microbiological outcome at day 7 & day 14

Liberation from MV at day 28

ICU mortality

Hospital mortality

28- day mortality

Incidence of new onset or worsening AKI

Incidence of bronchospasm during nebulization        

 

 


 
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