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CTRI Number  CTRI/2024/10/074924 [Registered on: 08/10/2024] Trial Registered Prospectively
Last Modified On: 27/01/2026
Post Graduate Thesis  No 
Type of Trial  Interventional 
Type of Study   Biological 
Study Design  Single Arm Study 
Public Title of Study   The purpose of the study is to assess the Safety and Efficacy of MyCART-01, a novel form of immunotherapy. Immunotherapy is a type of treatment that utilizes the bodys own immune system to fight diseases. In this case, MyCART-01 focuses on a specific protein called CD19 
Scientific Title of Study
Modification(s)  
A Prospective, Single-Arm, Open-Label, Multi-Center, Phase 1/2 Study of the Safety and Efficacy of MyCART-01 in Patients with CD19 positive Relapsed or Refractory B Cell Malignancies 
Trial Acronym  CART-NAL, SUMM1T 
Secondary IDs if Any  
Secondary ID  Identifier 
MCr_CART-NAL01_Version 2.0.0_03/06/2024  Protocol Number 
 
Details of Principal Investigator or overall Trial Coordinator (multi-center study)  
Name  Dr Dinesh Pendharkar 
Designation  Director and Head of Medical Oncology, Haemato-Oncology, Bone Marrow Transplantation. 
Affiliation  Sarvodaya Hospital, Faridabad 
Address  Sector-8, YMCA Rd, near Escorts Mujesar Metro Station, Sector 8, Faridabad, Haryana 121006.

Faridabad
HARYANA
121006
India 
Phone  9987736665  
Fax    
Email  drpendharkar@gmail.com  
 
Details of Contact Person
Scientific Query
 
Name  Dr Ashokkumar Thakkar 
Designation  Head of the Department - Clinical Research and Medical Writing 
Affiliation  Meril Life Science Pvt. Ltd 
Address  Bilakhia House, Survey No. 135/139, Muktanand Marg, Chala, Vapi-396191, Gujarat, India.

Valsad
GUJARAT
396191
India 
Phone  912603052100  
Fax    
Email  ashok.thakkar@merillife.com  
 
Details of Contact Person
Public Query

Modification(s)  
Name  Mukesh Kumar Saroj 
Designation  Senior Project Manager - Clinical Operations 
Affiliation  Micro CRISPR Pvt. Ltd. 
Address  Micro Crispr Pvt. Ltd, D-401, The First, B/S ITC Narmada Hotel Vastrapur, Ahmedabad - 380015, Gujarat.
Bilakhia House, Survey No. 135/139, Muktanand Marg, Chala, Vapi-396191, Gujarat, India.
Ahmadabad
GUJARAT
380015
India 
Phone  9768722945  
Fax    
Email  mukeshkumar.saroj@microcrispr.com  
 
Source of Monetary or Material Support  
Micro CRISPR, Bilakhia House, Survey No. 135/139, Muktanand Marg, Chala, Vapi-396191, Gujarat, India. 
 
Primary Sponsor  
Name  Micro CRISPR Private Limited 
Address  Survey No. 135/139, Bilakhia House, Muktanand Marg, Chala, Vapi, Valsad-396191, Gujarat, India 
Type of Sponsor  Pharmaceutical industry-Indian 
 
Details of Secondary Sponsor  
Name  Address 
NIL  NIL 
 
Countries of Recruitment     India  
Sites of Study
Modification(s)  
No of Sites = 13  
Name of Principal Investigator  Name of Site  Site Address  Phone/Fax/Email 
Dr Deepam Pushpam  All India Institute of Medical Sciences (AIIMS), New Delhi  Department of Oncology, Sri Aurobindo Marg, Ansari Nagar, Ansari Nagar East, New Delhi, Delhi 110029
New Delhi
DELHI 
9650629370

deepampushpam@gmail.com 
Dr Navile Aditya Murali  Apollo Hospital, Bangalore  154/11, opp. IIM, Bannerghatta road, Bengaluru-560076
Bangalore
KARNATAKA 
9629611281

bnadityamurali@gmail.com 
Lt Gen Dr Velu Nair  Apollo Hospitals International Ltd., Gandhinagar  Plot No. 1A, GIDC Estate, Bhat, Gandhinagar, Gujurat-382428, India
Gandhinagar
GUJARAT 
9818256670

nairvelu2000@yahoo.com 
Dr Jayachandran P K  Apollo Speciality Hospital, Chennai  320, Anna Salai, Rathna Nagar, Alwarpet, Chennai, Tamil Nadu 600035
Chennai
TAMIL NADU 
8148400246

dr.pkjayachandran@gmail.com 
Dr Revathi Raj  Apollo Speciality Hospital, Chennai  320, Anna Salai, Rathna Nagar, Alwarpet, Chennai, Tamil Nadu 600035
Chennai
TAMIL NADU 
9841070249

revaraj@yahoo.com 
Dr M Joseph John  Christian Medical College and Hospital, Ludhiana  Department of Clinical Haematology, Haemato-oncology & Bone marrow (stem cell) Transplantation, 4th Floor, Christian Medical College and Hospital Brown Road, Ludhiana, Punjab-141008, India
Ludhiana
PUNJAB 
8054959525

mjosephjohn@cmcludhiana.in 
Dr Suparno Chakrabarti  Dharamshila Narayana Superspeciality Hospital  Vasundhara Enclave, Near New Ashok Nagar Metro Station, New Delhi–110096
New Delhi
DELHI 
9871127809

supchak@gmail.com 
Dr Reetu Jain  Jaslok Hospital & Research Centre, Mumbai  Dr. Gopalrao Deshmukh Marg, Pedder Road, Mumbai, Maharashtra - 400026, India 
Mumbai
MAHARASHTRA 
9819095659

reebun@yahoo.com 
Dr Shrinath Manik Kshirsagar  K.J. somaiya hospital and research centre, Mumbai  CTRU Department, 3rd Floor, SS Building, K.J. Somaiya hospital and research centre, Somaiya Ayurvihar, SION( E ), Mumbai, 400022.
Mumbai
MAHARASHTRA 
9821556030

shrinath@somaiya.edu 
Dr Gauri Kapoor  Rajiv Gandhi Cancer Institute & Research Centre, Delhi  Sector-5, Rohini, Delhi-110085
New Delhi
DELHI 
9871114046

kapoor.gauri@gmail.com 
Dr Dinesh Pendharkar  Sarvodaya Hospital, Faridabad  Department of Oncology, Sector-8, YMCA Rd, near Escorts Mujesar Metro Station, Sector 8, Faridabad, Haryana 121006
Faridabad
HARYANA 
9987736665

drpendharkar@gmail.com 
Dr Harsha Pragnesh Panchal  The Gujarat Cancer and Research Institute  M.P Shah Cancer Hospital, New Civil Hospital Campus, Asarwa, Ahmedabad Gujarat- 380016
Ahmadabad
GUJARAT 
9825940769

harsha.panchal@gcriindia.org 
Dr Sandip Shah  Vedanta Institute of Medical Sciences  First Floor, Nr Samved Hospital Stadium Commerce Road Navrangapura, Ahmedabad-380009 India
Ahmadabad
GUJARAT 
9824041170

Sandip60@yahoo.com 
 
Details of Ethics Committee
Modification(s)  
No of Ethics Committees= 12  
Name of Committee  Approval Status 
Ethics Committee of CIMS (Care Institute of Medical Sciences)  Approved 
GCRI GCS Ethics Committee  Approved 
Institute Ethics Committee (IEC), AIl India Institute of Medical Sciences  Approved 
Institutional Ethics committee (IEC), Jaslok Hospital & Research Centre  Approved 
Institutional Ethics Committee - Clinical Studies, Apollo Hospitals International Ltd., Gandhinagar  Approved 
Institutional Ethics Committee Christian Medical College and Hospital  Approved 
Institutional Ethics Committee Dharamshila Narayana Superspeciality Hospital  Approved 
Institutional Ethics Committee-Clinical Studies, Apollo Hospital, Bangalore  Submittted/Under Review 
Institutional Ethics Committee-Clinical Studies, Apollo Hospitals, Chennai  Approved 
Institutional Review Board- Rajiv Gandhi Cancer Institute and Research Centre  Approved 
KJSMC Hospital & Research Center, (IEC)  Approved 
Sarvodaya Hospital and Research Centre Institutional Ethics Committee  Approved 
 
Regulatory Clearance Status from DCGI  
Status 
Approved/Obtained 
 
Health Condition / Problems Studied  
Health Type  Condition 
Patients  (1) ICD-10 Condition: C851||Unspecified B-cell lymphoma,  
 
Intervention / Comparator Agent  
Type  Name  Details 
Intervention  Mycabtagene autoleucel (MyCART-01)  MyCART-01 is a CD19-directed T cell immunotherapy. A single dose of MyCART-01 contains 0.2 to 5.0 x 106 CAR-positive viable T cells per kg of body weight for patients 100 kg or less, suspended in a patient-specific infusion bag for intravenous infusion. MyCART-01 administration will be single infusion process lasting for 1 hour and patient will be monitored for 2 year post MyCART-01 infusion.  
Comparator Agent  NA  NA 
 
Inclusion Criteria  
Age From  18.00 Year(s)
Age To  90.00 Year(s)
Gender  Both 
Details  Non Hodgkin’s Lymphoma:
1)Non Hodgkins Lymphoma Patients with age greater than or equal to 18 years.
2)Patients with relapsed or refractory B Cell NHL with histologically confirmed diagnosis of one of the following
a)DLBCL NOS
b)high grade B Cell lymphoma with MYC and BCL2 and or BCL6 rearrangements
c) transformed FL
d)mantle cell lymphoma
e)grade 3b FL
f)Richters transformation of CLL
g)Other forms of B cell NHL
Note:
i)For refractory disease patients must have progressive disease on last therapy, or have stable disease following at least 2 lines of therapy with duration of stable disease of less than or equal to 6 months
ii)For patients with transformed FL, patients must have received at least one line of chemotherapy for disease after transformation to DLBCL and have progressed or relapsed within 12 months
3) Refractory or relapsed disease, to prior first line therapy, including an anti CD20 monoclonal antibody and an anthracycline containing regimen
4) Patients with prior autologous hematopoietic stem cell transplant must not have traces of related toxicities.
5) At least 1 measurable lesion that is fluorodeoxy glucose positron emission tomography positive, as defined by Lugano criteria
Note: Previously irradiated lesions will be considered measurable only if progression is documented following g completion of radiation therapy. Acute Lymphoblastic leukemia

Acute Lymphoblastic Leukemia:
1)Adult patients of age greater than or equal to 18 years and children of age greater than or equal to 3 years
2)Diagnosed with the histologically confirmed CD19 positive relapsed or refractory adult or pediatric B Cell ALL
a)Refractory disease is defined by not achieving a CR after 2 cycles of a standard chemotherapy regimen or after completion of induction phase of pediatric regimens or
b)Relapsed disease as defined by bone marrow relapse at any point of time after achieving CR 1
3)Philadelphia chromosome positive Ph positive ALL patients
4)Confirmation from central pathology lab
i)Documentation of CD19 tumor expression in bone marrow by flow cytometryII
ii)Bone marrow with greater than or equal to 5 percent lymphoblasts
For all patients:
1)Eastern Cooperative Oncology Group performance status 0 to 1
2)Life expectancy greater than or equal to 12 weeks
3)Patients with absolute lymphocyte count of greater than or equal to 0.2 into 109 per L
4)Female patients of childbearing potential post menarcheal with an intact uterus and at least 1 ovary, who are less than 1 year postmenopausal must agree to use acceptable method of contraception from enrollment through at least 12 months after CAR T infusion. Male patients must agree to use effective contraception from enrollment through at least 12 months after CAR T infusion
5)Adequate organ function based on investigators discretion but not limited to the following points:
i)Renal: Estimated glomerular filtration rate greater than 50 ml min 1.73 m2
ii)Liver: Aspartate transaminase or alanine transaminase less than 3 upper limit of normal total bilirubin less than 1.5 ULN for patients with Gilberts syndrome total bilirubin less than 3 mg dL
iii)Cardiac: Hemodynamically stable and left ventricle ejection fraction greater than or equal to 45percent by echocardiogram
iv)Pulmonary Oxygen saturation level on room air greater than or equal to 91percent per pulse oximetry
6) Written voluntary informed consent (adults) or parental/guardian consent for children aged 3 to 11 and assent form for adolescent aged 12 to 17 years who are able to give written assent prior to performing any research related tests or procedures.
 
 
ExclusionCriteria 
Details  1)Eligible for and agrees to allogeneic HSCT
2)Treatment with the following therapies as described below
a) Prior treatment with any gene therapy or genetically modified cell therapy, including CAR-T Cells
b) Prior treatment with a CD19-directed antibody, bispecific T Cell engager, or antibody drug conjugate, unless there is confirmed CD19 expression by immunohistochemistry or flow cytometry after progression or relapse following most recent CD19 directed treatment.
3) Prior allogenic HSCT
4)Prior anaphylactic reaction to Cyclophosphamide, Fludarabine or any of the excipients of CART product
5) History of a seizure disorder, cerebrovascular ischemia or hemorrhage, dementia, cerebellar disease, or any autoimmune disease with CNS involvement
6)Unstable angina, clinically significant arrhythmia, or myocardial infarction within 12 months of enrollment
7) Presence of active bacterial, viral, or fungal infection
8)Positive for presence of human immunodeficiency virus type 1 or 2, or active hepatitis B virus orhepatitis C virus infection or other viral infections. Patients with prior history of hepatitis B or C infection who have documented undetectable viral load by quantitative polymerase chain reaction or nucleic acid testing may be permitted
9) Patients have active SARS COV-2 positive RT PCR test infection at the time of screening.
10) Any condition leading to T Cell depletion, rendering patient unsuitable for CAR T Cell therapy
11) Previous or concurrent malignancy, except basal cell or squamous cell skin carcinoma, adequately resected and in situ carcinoma of cervix, or a previous malignancy that was completely treated and has been in remission for greater than or equal to 5 years
12) Patient underwent radiation therapy before 14 days of enrollment
13) Use of systemic anti tumor therapy or investigational agent within 14 days or 5 half-lives, whichever is longer, of enrollment. An exception is made for prior inhibitory or stimulatory immune checkpoint molecule therapy, which is prohibited within 3 half lives of enrollment
14) Primary immunodeficiency disorder or active autoimmune disease requiring steroids and or other immunosuppressive therapy
15) Diagnosis of significant psychiatric disorder or other medical condition that, in the opinion of the investigator, could impede the patient’s ability to participate in the study
16) Women who are pregnant or breastfeeding
17) Any condition, in opinion of the Investigator or Screening committee, would preclude safe participation of patient in the study
 
 
Method of Generating Random Sequence   Not Applicable 
Method of Concealment   Not Applicable 
Blinding/Masking   Not Applicable 
Primary Outcome  
Outcome  TimePoints 
The incidence of adverse events, defined as safety evaluation
Evaluating the objective response rate (complete response (CR) + partial response(PR)) per the Lugano Response Criteria for malignant lymphoma
Evaluating the objective response rate in pediatric and young adult CD19+ relapsed or refractory B cell ALL, based on CR and complete remission with incomplete count recovery (CRi)  
30 Days 
 
Secondary Outcome  
Outcome  TimePoints 
Evaluating the objective response rate (complete response (CR) + partial response(PR)) per the Lugano Response Criteria for malignant lymphoma  3 Months 
Duration of response
Progression-free survival (central read)
Disease control rate [CR+PR+ stable disease (SD)]
Only for ALL patient:
Overall survival
Frequency and severity of adverse events and clinically significant laboratory
abnormalities
 
2 Years 
 
Target Sample Size   Total Sample Size="41"
Sample Size from India="41" 
Final Enrollment numbers achieved (Total)= "Applicable only for Completed/Terminated trials"
Final Enrollment numbers achieved (India)="Applicable only for Completed/Terminated trials" 
Phase of Trial   Phase 1/ Phase 2 
Date of First Enrollment (India)   28/10/2024 
Date of Study Completion (India) Applicable only for Completed/Terminated trials 
Date of First Enrollment (Global)  Date Missing 
Date of Study Completion (Global) Applicable only for Completed/Terminated trials 
Estimated Duration of Trial   Years="2"
Months="0"
Days="0" 
Recruitment Status of Trial (Global)   Not Applicable 
Recruitment Status of Trial (India)  Open to Recruitment 
Publication Details   N/A 
Individual Participant Data (IPD) Sharing Statement

Will individual participant data (IPD) be shared publicly (including data dictionaries)?  

Response - NO
Brief Summary  
This study is a prospective, single-arm, open-label, multi-center clinical trial designed to evaluate the safety and efficacy of MyCART-01 in patients with CD19+ relapsed or refractory B cell malignancies. The trial encompasses two phases, I and II, with a total intended enrollment of 41 participants, including both ALL (Acute Lymphoblastic Leukemia) and NHL (Non-Hodgkin Lymphoma) patients.

Phase I involves the inclusion of 10 patients, comprising 7 adults (with at least 1 from either ALL or NHL) and 3 pediatric patients with ALL. Following Phase I, Phase II aims to enroll an additional 31 patients, distributed between Stage I (14 patients) and Stage II (17 patients). 

The investigational product under evaluation is Mycabtagene Autoleaucel, denoted as MyCART-01. This therapy falls under the category of CAR-T (Chimeric Antigen Receptor T-cell) and is designed to target CD19, a protein expressed on the surface of B cells.

The study is currently in the I/II phase of development and is being conducted at approximately seven centers in India. The primary endpoint is to assess the safety and efficacy of MyCART-01 in treating CD19+ relapsed or refractory B cell malignancies.

Following the CAR-T infusion, participants will be monitored for a period of two years during the follow-up phase. The outcomes of this study will contribute valuable insights into the potential of MyCART-01 as a therapeutic intervention for patients with relapsed or refractory B cell malignancies.
 
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