Rationale: Regenerative endodontics deals with restoration of lost pulp-dentin complex using tissue engineering concepts. Appropriate selection of components of tissue engineering triad is essential. Literature shows that Stem cells from exfoliated deciduous teeth (SHED) and Nanohydroxyapatite derived from biowaste such as egg shells(E-nanoHA) has appreciable odontogenic properties. Injectable platelet rich fibrin(i-PRF) exhibits high release of growth factors, cellular migration and can three dimensionally take the shape of the canal. This study proposes to develop a single system of i-PRF scaffold with E-Nano HA and SHED for regeneration of pulp dentin complex

Novelty: Development of a single-phase injectable system of tissue engineering triad utilizing biological wastes such as exfoliated deciduous teeth, egg shells for pulp-dentin regeneration.  

Objectives: Primary:1. Preparation and characterization of E-nanoHA 2. Preparation of  culture of stem cells derived from exfoliated deciduous teeth and to develop experimental injectable platelet rich fibrin scaffold loaded with E nano HA and seeded with SHED 3. Assesing In-vitro bioactivity and cytotoxicity of the developed scaffold using pre-clinical animal model and clinical efficacy using a double-blind randomised control trial. Secondary: To develop a technology transfer package for the newly developed scaffold

Methods: Microwave processed E-nanoHA prepared from egg shell, characterized using SEM, EDAX, XRD and cultured SHED cells would be seeded into the injectable fibrin scaffold. In-vitro bioactivity studies and MTT assay would be done to assess the cytotoxicity. Preclinical animal studies would be done followed by a double-blind randomised control trial comparing the developed scaffold with that of the conventional pulp regeneration method. Data collection and statistical analysis of the data would be done.