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CTRI Number  CTRI/2023/11/059617 [Registered on: 07/11/2023] Trial Registered Prospectively
Last Modified On: 22/12/2025
Post Graduate Thesis  No 
Type of Trial  Interventional 
Type of Study   Vaccine 
Study Design  Randomized, Parallel Group, Placebo Controlled Trial 
Public Title of Study   A Global Study to find out the Effects of a Test Vaccine on prevention of harmful diseases caused by E.Coli spreading outside of the gut in Adults who are 60 Years And Older with a History of Urinary Tract Infection in the Past 2 Years. 
Scientific Title of Study   Randomized, Double-blind, Placebo-controlled, Multicenter Phase 3 Study to Assess the Efficacy, Safety And Immunogenicity of Vaccination With ExPEC9V in the Prevention of Invasive Extraintestinal Pathogenic Escherichia Coli Disease in Adults Aged 60 Years And Older With a History of Urinary Tract Infection in the Past 2 Years 
Trial Acronym  Embrace 
Secondary IDs if Any  
Secondary ID  Identifier 
2020-005273-27  EudraCT 
NCT04899336  ClinicalTrials.gov 
VAC52416BAC3001 Amendment 5, dated 02 December 2022  Protocol Number 
 
Details of Principal Investigator or overall Trial Coordinator (multi-center study)  
Name   
Designation   
Affiliation   
Address 




 
Phone    
Fax    
Email    
 
Details of Contact Person
Scientific Query
 
Name  Dr Sanish Davis 
Designation  R and D Director GCO India 
Affiliation  Johnson and Johnson Private Limited 
Address  Arena Space Behind Majas Depot Off J V Link Road Jogeshwari East,

Mumbai (Suburban)
MAHARASHTRA
400060
India 
Phone  9820958943  
Fax    
Email  SDavis20@ITS.JNJ.com  
 
Details of Contact Person
Public Query
 
Name  Dr Sanish Davis 
Designation  R and D Director GCO India 
Affiliation  Johnson and Johnson Private Limited 
Address  Arena Space Behind Majas Depot Off J V Link Road Jogeshwari East,


MAHARASHTRA
400060
India 
Phone  9820958943  
Fax    
Email  SDavis20@ITS.JNJ.com  
 
Source of Monetary or Material Support  
Janssen Vaccines & Prevention B.V., Archimedesweg 4, 2333 CN Leiden, The Netherlands. 
 
Primary Sponsor  
Name  Janssen Vaccines & Prevention B.V. 
Address  Archimedesweg 4, 2333 CN Leiden, The Netherlands 
Type of Sponsor  Pharmaceutical industry-Global 
 
Details of Secondary Sponsor  
Name  Address 
Pharmaceutical Research Associates India Pvt Ltd  Regus Kaledonia, Unit No 1B, Office No. 538, 5th Floor, Sahar Road, Off Western Express Highway, Andheri East, Mumbai- 400069 
 
Countries of Recruitment     Australia
Canada
China
Colombia
Czech Republic
Denmark
France
Germany
India
Israel
Italy
Japan
Republic of Korea
Netherlands
New Zealand
Spain
Sweden
Taiwan
Thailand
United Kingdom
United States of America  
Sites of Study  
No of Sites = 13  
Name of Principal Investigator  Name of Site  Site Address  Phone/Fax/Email 
Dr Deepak Varade  Asian Institute of Medical Science  Department of Medicine, P/72, Milap Nagar, MIDC, Dombivli (E)-421203
Mumbai
MAHARASHTRA 
9870409142

deepak.varade@gmail.com 
Dr Rohidas Tanku Borse  B.J. Government Medical College & Sassoon General Hospitals  Head of medicine department,3rd floor, Sasson Main Building, B.J. Government Medical College & Sassoon General Hospitals, Sassoon Road, Somwar Peth, Pune-411001.
Pune
MAHARASHTRA 
8793226922

drborser@gmail.com 
Dr Parshottam Koradia  BAPS Pramukh Swami Hospital  Clinical Research Department, Ground Floor, Shri Pramukh Swami Maharaj Marg, Adajan Char Rasta, Adajan, Surat-395009, Gujarat
Surat
GUJARAT 
0261-2557577

ceobapshospital@gmail.com 
Dr Viny Kantroo  Indraprastha Apollo Hospitals  Department of Critical Care and Sleep Medicine, Sarita Vihar Mathura Road New Delhi 110076.
South
DELHI 
01171791091

vinykantroo@gmail.com 
Dr Tejeswini CJ  JSS Hospital  Department of Geriatric,1st floor, Mahatma Gandhi road, Ramachandra agrahara, Mysore-570004, Karnataka
Mysore
KARNATAKA 
9980048080

tejaswinicj@jssuni.edu.in 
Dr Madhav Prabhu  KLES Dr Prabhakar Kore Hospital & Medical Research Centre  OPD No 11, Ground floor, KLES Dr Prabhakar Kore Hospital & Medical, Research Centre, Nehru Nagar, Belagavi-590010 Karnataka
Belgaum
KARNATAKA 
9341101268

Maddy2380@gmail.com 
Dr Kapil Thakkar  Nirmal Hospital Pvt. Ltd,  Clinical Research Department, 5th floor, Ring Road, Surat-395002
Surat
GUJARAT 
02612236036

kapilthakkar@gmail.com 
Dr Badal Kumar Sahu  NRS Medical College & Hospital,  Department of General Medicine,1st floor, Room No 8, OPD Building, NRS Medical College & Hospital, 138 A.J.C Bose Road, Kolkata-700020, West Bengal
Kolkata
WEST BENGAL 
9414459790

badalksahu@gmail.com 
Dr Deepak Dewan  Regency Health SuperSpeciality Hospital,  Room No 11, Basement, Regency Health Super Specialty Hospital, Tedhi Pulia, Ring Road, Khurram Nagar, Lucknow-226022.
Lucknow
UTTAR PRADESH 
9936507362

drdeepakdewan@rediffmail.com 
Dr Mukesh Kalla  S.R. Kalla Memorial Gastro & General Hospital  Gastroenterology department, Basement,78-79 Dhuleshwar Garden, Behind HSBC Bank, Sardar Patel Marg C-Scheme, Jaipur-302001, Rajasthan
Jaipur
RAJASTHAN 
01414039432

drmkalla@rediffmail.com 
Dr Mayank Thakker  Shree Giriraj Multispecialty Hospital, A Unit of Shree Giriraj Lifecare Private Limited,  CRC department ,1st floor, Shree Giriraj Multi-specialty Hospital, A Unit of Shree Giriraj Lifecare Private, Limited, 27- Navjyot Park Corner, 150 feet Ring Road, Rajkot 360005.
Ahmadabad
GUJARAT 
9909971118

drmayankthakker@gmail.com 
Dr GirishB   Shri Atal Bihari Vajpayee Medical College and research Institute, Bowring and Lady Curzon Hospital  Room 01, 1st Floor, Admin Block annex, Shri Atal Bihari Vajpayee Medical College and research Institute, Bowring and Lady Curzon Hospital, Bangalore 560001
Bangalore
KARNATAKA 
9113030135

drgirishsabvmcri@gmail.com 
Dr Dhananjai agrawal  SMS Superspeciality Hospital, Attached to SMS Medical College  Room No: 507, 5th Floor, Department of nephrology, SMS Superspecialty Hospital, Attached to SMS Medical College, Vivekanand Marg, C-Scheme, Jaipur-302001
Jaipur
RAJASTHAN 
9414459790

dhananjaynephro@gmail.com 
 
Details of Ethics Committee  
No of Ethics Committees= 13  
Name of Committee  Approval Status 
BAPS Pramukh Swami Hospital Institutional Ethics Committee, BAPS Pramukh Swami Hospital, Shri Pramukh Swami Maharaj Marg, Adajan Char Rasta, Adajan, Surat-395009, Gujarat  Approved 
Ethics Committee of SABVMCRI (Shri Atal Bihari Vajpayee Medical College and Research Institute)  Submittted/Under Review 
Institutional Ethics Committee - Clinical Studies, Indraprastha Apollo Hospitals Sarita Vihar Mathura Road New Delhi 110076.  Approved 
Institutional Ethics Committee Regency Hospital, Pusa Road, Khurram Nagar, Lucknow, Uttar Pradesh - 226022  Approved 
Institutional Ethics Committee SMS Medical College & Attached Hospital, Office of Ethics Committee, Second Floor, New Academic Block, SMS Medical college, jaipur-302004, Rajasthan  Submittted/Under Review 
Institutional Ethics committee, JSS Medical college & Hospital, 3rd floor, JSS Medical College, Mysore-570015, Karnataka  Approved 
Institutional Ethics Committee, B.J.G.M.C. & Sassoon General Hospitals, Sassoon Road, Station Road, Pune-411001  Submittted/Under Review 
Institutional Ethics Committee, KLE Universitys KLE Dr PK Hospital & MRC, Nehru Nagar Belagavi-590010  Approved 
Institutional Ethics Committee, NRS Medical College & Hospital,138 A.J.C Bose Road, Kolkata700020, West Bengal  Approved 
Nirmal Hospital Ethics Committee, Nirmal Hospital Pvt. Ltd, Ring Road, Surat-395002  Approved 
S.R. Kalla Memorial Ethical Committee for Human Research, S.R. Kalla Memorial Gastro & General Hospital, 78-79 Dhuleshwar Garden, Behind HSBC Bank, Sardar Patel Marg C-Scheme, Jaipur-302001, Rajasthan.  Approved 
Shree Giriraj Hospital Research Ethics Committee,Shree Giriraj Multispeciality Hospital, 27- Navjyot Park Corner| 150 feet Ring Road, Rajkot 360005.  Approved 
Suraksha Ethics Committee, Asian Institute of Medical Science, P/72, Milap Nagar, MIDC, Dombivli (E)421203  Approved 
 
Regulatory Clearance Status from DCGI
Modification(s)  
Status 
Notified 
 
Health Condition / Problems Studied  
Health Type  Condition 
Patients  (1) ICD-10 Condition: A488||Other specified bacterial diseases,  
 
Intervention / Comparator Agent  
Type  Name  Details 
Intervention  ExPEC9V Vaccine  Participants will receive a single intramuscular (IM) injection of 9-valent extraintestinal pathogenic Escherichia coli vaccine (ExPEC9V) on Day 1. There will be a total 7 follow ups/visits post vaccination over the period of 3 years. 
Comparator Agent  Placebo  Participants will receive a single IM injection of matching placebo on Day 1.There will be a total 7 follow ups/visits post vaccination over the period of 3 years. 
 
Inclusion Criteria  
Age From  60.00 Year(s)
Age To  99.00 Year(s)
Gender  Both 
Details  1- Participant must be willing to share relevant medical information pertaining to medical history and to share medical records relevant to the medical events identified as suspected cases of invasive extraintestinal pathogenic Escherichia coli disease (IED), urinary tract infections (UTI), or acute bacterial prostatitis (ABP) occurring during the study observation period.
2- Participant must have a history of UTI in the past 2 years for which evidence of diagnosis was verified by the investigator. In case of a recent history of UTI, the condition must have resolved greater than (>)14 days prior to randomization.
3- Before randomization, participants who were born female must be either postmenopausal or permanently sterile, and not intending to conceive by any methods.
4- Participant must be willing to provide verifiable identification, has means to be contacted and to contact the investigator during the study.
 
 
ExclusionCriteria 
Details  1- Participant has end stage renal disease for which dialysis is required.
2- Participant has a contraindication to intramuscular (IM) injections and blood draws example, due to bleeding disorders or a history of difficult blood draws.
3- Chronic systemic prophylactic use of antibacterials (including antibiotics and urinary tract antiseptics). Repeated courses administered for recurrent UTIs preceding randomization will not be referred to as chronic. In case of chronic prophylactic use of antibacterials, the last dose must have been administered > 60 days prior to randomization.
4- Participant has a history of acute polyneuropathy (for example, Guillain barre syndrome) or chronic inflammatory demyelinating polyneuropathy.
5- Participant has received any Escherichia coli (E. coli) or extraintestinal pathogenic Escherichia coli (ExPEC) vaccine 
 
Method of Generating Random Sequence   Stratified randomization 
Method of Concealment   Centralized 
Blinding/Masking   Participant and Investigator Blinded 
Primary Outcome  
Outcome  TimePoints 
1-Number of Participants with First Invasive Extraintestinal Pathogenic E. coli Disease(IED) Event with Microbiological Confirmation in Blood, Other Sterile Sites, or Urine, Caused by 9 valent Extraintestinal Pathogenic E. coli Vaccine(ExPEC9V) O serotypes
2-Number of Participants with First IED Event with Microbiological Confirmation in Blood or Other Sterile Sites Caused by ExPEC9V O serotypes  
Up to 3 years  
 
Secondary Outcome  
Outcome  TimePoints 
Number of Participants with All IEDs (Including Multiple IEDs per Participant) Caused by ExPEC9V O-serotypes   Up to 3 years  
Number of Participants with First Hospitalized IED Event Caused by ExPEC9V O-serotypes  Up to 3 years  
Number of Participants with First IED Event Meeting Criteria for Sepsis Caused by ExPEC9V O-serotypes   Up to 3 years  
Number of Participants with First Bacteremic IED Event Caused by ExPEC9V O-serotypes   Up to 3 years  
Number of Participants with First Pyelonephritis Event Caused by ExPEC9V O-serotypes   Up to 3 years  
Number of Participants with First Urinary Tract Infection (UTI) Event Caused by ExPEC9V O-serotypes   Up to 3 years  
Number of Participants with All UTIs (Including Multiple UTIs per Participant) Caused by ExPEC9V O-serotypes   Up to 3 years  
Number of Participants with First IED Event Caused by Any ExPEC O-serotype   Up to 3 years 
Number of Participants with First Pyelonephritis Event caused By Any ExPEC O-serotype   Up to 3 years  
Number of Participants with First UTI Event caused by Any ExPEC O-Serotype  Up to 3 years 
Antibody Titers to Vaccine O-serotype Antigens as Determined by Multiplex Electrochemiluminescent (ECL)-based Immunoassay   Up to 3 years  
Antibody Titers to Genetically Detoxified Form of Exotoxin A Derived from Pseudomonas Aeruginosa (EPA) as Determined by Multiplex ECL-based Immunoassay   Up to 3 years 
Antibody Titers to Vaccine O-serotype Antigens as Determined by Multiplex Opsonophagocytic Assay (MOPA)   Up to 3 years  
Number of Participants with Solicited Local Adverse Events (AEs)   Up to Day 15 (until 14 days post-vaccination) 
Number of Participants with Solicited Systemic AEs   Up to Day 15 (until 14 days post-vaccination) 
Number of Participants with Unsolicited AEs   Up to Day 30 (until 29 days post-vaccination) 
Number of Participants with Serious Adverse Events (SAEs)  Up to 3 years  
Short Form-36 (SF-36) Total Scores   Up to 3 years  
European Quality of Life (EuroQol) 5-Dimension 5-Level Questionnaire (EQ-5D-5L) Scores   Up to 3 years  
Change from Baseline in Frailty Index   Baseline to 3 years  
Medical Resource Utilization (MRU) for IED Events   Up to Day 366 Post-IED  
MRU for UTI Events   Up to Day 29 Post-UTI  
MRU for Acute Bacterial Prostatitis (ABP)   Up to Day 29 Post-ABP  
Number of Participants with Hospitalization for IED or, UTI, or ABP Events   Up to 3 years 
Length of Stay in Hospital for IED, UTI, or ABP Events   Up to 3 years  
Number of Participants with IED-related & All-cause Mortality   Up to 3 years  
 
Target Sample Size   Total Sample Size="18556"
Sample Size from India="450" 
Final Enrollment numbers achieved (Total)= "Applicable only for Completed/Terminated trials"
Final Enrollment numbers achieved (India)="Applicable only for Completed/Terminated trials" 
Phase of Trial   Phase 3 
Date of First Enrollment (India)   22/01/2024 
Date of Study Completion (India) Applicable only for Completed/Terminated trials 
Date of First Enrollment (Global)  30/06/2021 
Date of Study Completion (Global) Applicable only for Completed/Terminated trials 
Estimated Duration of Trial   Years="3"
Months="0"
Days="0" 
Recruitment Status of Trial (Global)
Modification(s)  
Other (Terminated) 
Recruitment Status of Trial (India)  Other (Terminated) 
Publication Details   N/A 
Individual Participant Data (IPD) Sharing Statement

Will individual participant data (IPD) be shared publicly (including data dictionaries)?  

Response - NO
Brief Summary
Modification(s)  

Invasive extraintestinal pathogenic Escherichia coli (ExPEC) disease is defined as an acute illness consistent with systemic bacterial infection, which is microbiologically confirmed either by the isolation and identification of Escherichia coli (E. coli) from blood or any other sterile body sites, or by the isolation and identification of E. coli from urine in a participant with urosepsis and no other identifiable source of infection. ExPEC9V (JNJ-78901563, primary compound number: VAC52416) is a 9-valent vaccine candidate in development for active immunization for the prevention of IED in adults 60 years of age and older. Although IED affects all ages, adults aged greater than or equal to (>=) 60 years have an increased risk of developing IED, including bacteremia and sepsis, which can be community-acquired, hospital-acquired or healthcare associated. As the mechanism of action of conjugate vaccines in the prevention of invasive disease is not expected to be affected by antibiotic resistance mechanisms, that is, resistance mechanisms are not linked to O-polysaccharide structures, the sponsor has no reason to expect a difference in outcome of efficacy between antimicrobial-resistant and susceptible O-serotypes. This study incorporates an inferentially seamless group-sequential design. This study consists of a Screening Phase (selected screening procedures may be performed up to 8 days prior to vaccination on Day 1), Vaccination Phase (Day 1) and Follow-up Phase (up to 3 years post-vaccination). The total study duration is up to 4 years and 2 months. Key safety assessments include serious adverse events (SAEs), solicited and unsolicited adverse events (AEs), physical examination, and vital signs


 
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