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CTRI Number  CTRI/2023/10/058660 [Registered on: 13/10/2023] Trial Registered Prospectively
Last Modified On: 13/12/2024
Post Graduate Thesis  No 
Type of Trial  Interventional 
Type of Study   Drug 
Study Design  Randomized, Parallel Group, Multiple Arm Trial 
Public Title of Study   A study to evaluate Safety,Efficacy,Tolerability, Pharmacokinetics, and Pharmacodynamics of ZYIL1 in patients with Amyotrophic Lateral Sclerosis 
Scientific Title of Study   A phase 2, proof-of-concept, placebo controlled, randomized, multi-centre, double blind study of ZYIL1 to evaluate the efficacy, safety, tolerability, pharmacokinetics and pharmacodynamics in patients with Amyotrophic Lateral Sclerosis (ALS) 
Trial Acronym  NIL 
Secondary IDs if Any  
Secondary ID  Identifier 
ZYIL.23.003 Ver 01 Dated 27 May 2023  Protocol Number 
 
Details of Principal Investigator or overall Trial Coordinator (multi-center study)  
Name  Dr Maulik Doshi 
Designation  General Manager 
Affiliation  Zydus lifesciences Ltd (Formerly Cadila Healthcare Ltd.) 
Address  Zydus Research Center, Survey No. 396/403, Sarkhej-Bavla National Highway No. 8A Moraiya, Ahmedabad - 382213

Ahmadabad
GUJARAT
382213
India 
Phone  02717665555  
Fax    
Email  Maulik.Doshi@zydusLife.com  
 
Details of Contact Person
Scientific Query
 
Name  Dr Bhargav Darji 
Designation  Senior Medical Expert 
Affiliation  Zydus lifesciences Ltd (Formerly Cadila Healthcare Ltd.) 
Address  Zydus Research Centre, Survey No. 396/403, Sarkhej-Bavla National Highway No.8A Moraiya

Ahmadabad
GUJARAT
382213
India 
Phone  02717665555  
Fax    
Email  Bhargav.R.Darji@ZydusLife.com  
 
Details of Contact Person
Public Query
 
Name  Dr Bhargav Darji 
Designation  Senior Medical Expert 
Affiliation  Zydus lifesciences Ltd (Formerly Cadila Healthcare Ltd.) 
Address  Zydus Research Centre, Survey No. 396/403, Sarkhej-Bavla National Highway No.8A Moraiya

Ahmadabad
GUJARAT
382213
India 
Phone  02717665555  
Fax    
Email  Bhargav.R.Darji@ZydusLife.com  
 
Source of Monetary or Material Support  
Zydus Lifesciences Limited Zydus Research Centre, Survey No. 396/403, Sarkhej-Bavla National Highway No. 8A Moraiya, Ahmedabad – 382213 
 
Primary Sponsor  
Name  Zydus Lifesciences Limited 
Address  Zydus Corporate Park, Scheme No. 63, Survey No. 536, Khoraj (Gandhinagar), Nr.Vaishnodevi Circle, Sarkhej-Gandhinagar Highway, Ahmedabad-382481, Gujarat,India 
Type of Sponsor  Pharmaceutical industry-Indian 
 
Details of Secondary Sponsor  
Name  Address 
NIL  NIL 
 
Countries of Recruitment     India  
Sites of Study
Modification(s)  
No of Sites = 9  
Name of Principal Investigator  Name of Site  Site Address  Phone/Fax/Email 
Dr Biman Kanti Ray  Bangur Institute of Neurosciences, IPGME&R and SSKM Hospital  52/1A, Sambhunath Pandit Street, Kolkata-700025
Kolkata
WEST BENGAL 
9433185327

biman.kanti@rediffmail.com 
Dr Venkataramana N K  BrainS Super Speciality Hospital  " #192, TMH _ Mahabodhi Health Center, T. Mariyappa Road, Lalbagh, Siddapura, Jayanagar 1st Block, Bengaluru, Karnataka 560011, India."
Bangalore
KARNATAKA 
9845030906

drnkvr@brains.org.in 
Dr Doshi Suyog Ashok  CIMETs Imandar Multispeciality Hospital  S. No. 15, Behind KPCT mall, Fatima nagar, Wanavadi, Pune- 411040
Pune
MAHARASHTRA 
9503690498

suyog313@gmail.com 
Dr Pratik Uttarwar  KIMS-Kingsway Hospitals  Medisearch Lifesciences Pvt. Ltd, 44, SPANV, Kingsway Rd, near Kasturchand Park, Nagpur, Maharashtra - 440001, India.
Nagpur
MAHARASHTRA 
9179288227

pratikuttarwar1@gmail.com 
Dr Vyankatesh Bolegave  Rajiv Gandhi Medical college & Chatrapthi Shivaji Maharaj Hospital  " Budhaji Nagar, Old Thane, Belapur road, Kalwa, Thane- 400605"
Thane
MAHARASHTRA 
9869694862

bolegavenkatesh@gmail.com 
Dr Rana Kaushikkumar Ramanlal  Rhythm heart institute  Near Siddharth Bunglows, Sama-savli road, Vadodara- 390022
Vadodara
GUJARAT 
9909502349

drkaushikrana.cr@gmail.com 
Dr Laxmi Khanna  Sir Ganga Ram Hospital  Sir Ganga Ram Hospital Marg Rajinder Nagar, New Delhi-60
New Delhi
DELHI 
9873558121
011-45041726
laxmikhanna@hotmail.com 
Dr Amit Yeole  Surya Multispecialty Hospital  Surya Arcade, 2nd floor, Opp. Nimani Bus stand, Panchavati, Nashik- 422003
Nashik
MAHARASHTRA 
7588554530

Amit_Yeole37@rediffmail.com 
Dr Arvind Sharma  Zydus Hospitals & Healthcare Research  Zydus Hospitals & Healthcare Research Pvt. Ltd., Zydus Hospitals Road, S G Highway, Thaltej, Ahmedabad- 380054
Ahmadabad
GUJARAT 
9409210486

ARVINDSHARMA@zydushospitals.com 
 
Details of Ethics Committee
Modification(s)  
No of Ethics Committees= 10  
Name of Committee  Approval Status 
"ACE Independent Ethics Committee 2nd Floor B Portion, Nandanam Building, No. 1, B Channasandra, OMBR 5TH Main Road, Bangalore 560043, Karnataka, India "   Approved 
"Ethics Committee Inamdar Multispeciality Hospital CIMETs Inamdar Mutispeciality Hospital. Hospital Building S.No .15,Fatima Nagar ,Wanawadi. Pune Pune Maharashtra - 411040 India"   Approved 
"Institutional Clinical Ethics Committee RGMC and Chatrapati Shivaji Maharaj Hospital. Thane-Belapur Rd, Kalwa, Thane-400605 Thane- Belapur Rd, Kalwa, Thane-400605 Thane Thane Maharashtra - 400605 India"   Submittted/Under Review 
"IPGMEandR Resaerch Oversight Committee IPGMEandR 244 Acharya J. C. Bose Road - Kolkata Kolkata West Bengal - 700020 India "   Approved 
"Kingsway Hospitals Ethics Committee 44, Kingsway, Near Kasturchand park, Nagpur, Maharashtra, India- 440001"   Approved 
"Leelavati Ethics Committee Leelavati Care Hospital, Near Big Bazar College Road, Nashik, Maharashtra - 422005, India"   Approved 
"Rhythm Heart Institute Ethics Committee Rhythm Heart Institute, Near Siddharth Bunglows Sama- Savli Road VADODARA Vadodara Gujarat - 390022 India"   Approved 
"Sir Ganga Ram Hospital Ethics Committee, Sir Ganga Ram Hospital, old Rajinder nagar, New Delhi- 110060, India"   Approved 
"Zydus Hospital Ethics Committee Zydus Hospitals & Healthcare Research Pvt Ltd., Zydus Hospital Road, Near Sola Bridge S. G. Highway Thaltej Ahmedabad Gujarat - 380054 India"   Approved 
IPGMEandR Resaerch Oversight Committee IPGMEandR 244 Acharya J. C. Bose Road - Kolkata Kolkata West Bengal - 700020 India   Approved 
 
Regulatory Clearance Status from DCGI  
Status 
Approved/Obtained 
 
Health Condition / Problems Studied  
Health Type  Condition 
Patients  (1) ICD-10 Condition: G121||Other inherited spinal muscular atrophy,  
 
Intervention / Comparator Agent  
Type  Name  Details 
Comparator Agent  Placebo of ZYIL1 Capsules  Route :- Oral Strength : 25 and 50 mg Duration : 56 days 
Intervention  ZYIL1 Capsules  Route :- Oral Strength : 25 and 50 mg Duration : 56 days 
 
Inclusion Criteria  
Age From  18.00 Year(s)
Age To  80.00 Year(s)
Gender  Both 
Details  1.Male and/or female patients aged between 18 and 80 years (inclusive at screening).
2. Diagnosis of probable or definite ALS, according to the revised version of the El Escorial
World Federation of Neurology criteria. Refer Appendix III - El Escorial Criteria.
3. Time since onset# of first symptom of ALS ≤9 months
4. Slow Vital Capacity (SVC) ≥ 50% of the predicted value
5. Be able to swallow the study capsules during study
6. Either not currently receiving riluzole or on a stable dose of riluzole for at least 4 weeks
before the screening visit. Participants receiving riluzole are expected to remain on the
same dose throughout the duration of the study
7. Either not currently receiving edaravone or on edaravone treatment. Participants
receiving edaravone must have completed at least 1 cycle of treatment before the
screening visit and are expected to continue with stable dose edaravone treatment
throughout the duration of the study 
 
ExclusionCriteria 
Details  1)With significant cognitive impairment, psychiatric disease, other neurodegenerative
disorder (e.g., Parkinson disease or AD), substance abuse other causes of neuromuscular weakness, or any other condition that would make the participants unsuitable for
participating in the study or could interfere with assessment or completing the study in
the opinion of the Investigator
2. History of recent serious infection (e.g., pneumonia, septicemia) within 4 weeks of the
screening visit; infection requiring hospitalization or treatment with IV antibiotics,
antivirals, or antifungals within 4 weeks of screening; or chronic bacterial infection (such
as tuberculosis) deemed unacceptable as per the Investigator’s judgment
3. With active herpes zoster infection within 2 months prior to the screening visit
4. A documented history of attempted suicide within 6 months prior to the screening visit,
or in the Investigator’s judgment are at risk for a suicide attempt
5. History of unstable or severe cardiac, pulmonary, oncological, hepatic, or renal disease
or another medically significant illness other than ALS precluding their safe
participation in this study
6. Participants who are pregnant or are currently breastfeeding
7. A known history of allergy to any ingredients of ZYIL1
8. Patients taking concomitant medicines within 7 days or 5 half-lives of the medication
(whichever is longer) prior to first dose of study drug administration till end of the study,
which are substrate of CYP1A2 enzymes (e.g., alosetron, caffeine, duloxetine,
melatonin, ramelteon, tasimelteon, tizanidine etc.) and CYP2B6 enzymes (e.g.,
bupropion, efavirenz etc.).
9. Use of any steroids, colchicine or anti-IL-1 inhibitors within 7 days or 5 half-lives of the
medication (whichever is longer) prior to first dose of study drug administration.
10. Use of any investigational drugs concurrently or within 4 weeks or 5 half-lives
(whichever is longer), prior to first dose of study drug administration.
11. Any clinically significant and/or laboratory significant value or other instability that
would prevent the patient from participating in the study as determined by the
Investigator.
12. Received a live vaccine within 14 days before the screening visit or planning to receive
during the study
13. Participants who have received stem cell or gene therapy for ALS at any time in the past 
 
Method of Generating Random Sequence   Computer generated randomization 
Method of Concealment   On-site computer system 
Blinding/Masking   Participant and Investigator Blinded 
Primary Outcome  
Outcome  TimePoints 
To measure the efficacy of the treatment using the slope of progression with the revised
Amyotrophic Lateral Sclerosis Functional Rating Scale 
Baseline to Day 84 
 
Secondary Outcome  
Outcome  TimePoints 
safety & tolerability of ZYIL1 in patients with ALS  Baseline to Day 84 
evaluate pharmacokinetics of ZYIL1  Baseline to Day 84 
evaluate effect of ZYIL1 on neurofilament biomarker  Baseline to Day 84 
 
Target Sample Size   Total Sample Size="24"
Sample Size from India="24" 
Final Enrollment numbers achieved (Total)= "24"
Final Enrollment numbers achieved (India)="24" 
Phase of Trial   Phase 2 
Date of First Enrollment (India)   30/10/2023 
Date of Study Completion (India) 26/06/2024 
Date of First Enrollment (Global)  Date Missing 
Date of Study Completion (Global) Date Missing 
Estimated Duration of Trial   Years="1"
Months="0"
Days="0" 
Recruitment Status of Trial (Global)
Modification(s)  
Not Applicable 
Recruitment Status of Trial (India)  Completed 
Publication Details   N/A 
Individual Participant Data (IPD) Sharing Statement

Will individual participant data (IPD) be shared publicly (including data dictionaries)?  

Response - NO
Brief Summary  
ZYIL1 is expected to show benefit in patients with Amyotrophic Lateral Sclerosis (ALS). The present study aims to determine the safety, tolerability, pharmacokinetics, and pharmacodynamics of ZYIL1 when administered to subjects with ALS. 

This is a proof-of-concept, placebo controlled, randomized, double blind study designed to evaluate the efficacy, safety, tolerability, pharmacokinetics, and pharmacodynamics in patients with Amyotrophic Lateral Sclerosis following a twice daily oral administration of ZYIL1 or matching placebo. Twenty-four (24) patients will be randomly assigned in a 1:1:1:1 ratio to oral twice daily capsules of ZYIL1 25 mg or ZYIL1 50 mg or ZYIL1 75 mg or matching placebo. Patients on ZYIL1 25 mg and 50 mg arm along with matching placebo will be randomized first. Treatment duration will be twelve (12) weeks.
 
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