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CTRI Number  CTRI/2023/06/054253 [Registered on: 21/06/2023] Trial Registered Prospectively
Last Modified On: 08/01/2026
Post Graduate Thesis  No 
Type of Trial  Interventional 
Type of Study   Drug
Other (Specify) [Topical Solution]  
Study Design  Randomized, Parallel Group, Active Controlled Trial 
Public Title of Study   Phase III Clinical trial of WOXheal® Diperoxochloric Acid [DPOCL] in the treatment of chronic venous leg ulcers 
Scientific Title of Study   A multicenter, randomized, double-blind, active-control phase III clinical trial of WOXheal® [Diperoxochloric acid] topical solution in the treatment of chronic venous leg ulcer – in comparison with the active-control 0.9% sodium chloride topical solution in addition to standard of care. 
Trial Acronym   
Secondary IDs if Any  
Secondary ID  Identifier 
LCT-01/21, version no. 3.0, dated 20-Oct-2022  Protocol Number 
 
Details of Principal Investigator or overall Trial Coordinator (multi-center study)  
Name  Dr Mukund Zarapkar  
Designation  Vice President- Clinical 
Affiliation  LifeSan Clinical Research, division of Centaur Pharmaceuticals Pvt.Ltd. 
Address  5th and 6th floor, B-wing, Centaur House, Near Hotel Grand Hyatt, Santacruz East, Mumbai 400055, Maharashtra, India.

Mumbai (Suburban)
MAHARASHTRA
400055
India 
Phone  91-22-66499154  
Fax    
Email  drzarapkar@lifesan.in  
 
Details of Contact Person
Scientific Query
 
Name  Dr Mukund Zarapkar  
Designation  Vice President- Clinical 
Affiliation  LifeSan Clinical Research, division of Centaur Pharmaceuticals Pvt.Ltd. 
Address  5th and 6th floor, B-wing, Centaur House, Near Hotel Grand Hyatt, Santacruz East, Mumbai 400055, Maharashtra, India.


MAHARASHTRA
400055
India 
Phone  91-22-66499154  
Fax    
Email  drzarapkar@lifesan.in  
 
Details of Contact Person
Public Query
 
Name  Mr Rajendra M Sawant 
Designation  Executive-Clinical Trial 
Affiliation  LifeSan Clinical Research, division of Centaur Pharmaceuticals Pvt.Ltd. 
Address  5th and 6th floor, B-wing, Centaur House, Near Hotel Grand Hyatt, Santacruz East, Mumbai 400055, Maharashtra, India.

Mumbai (Suburban)
MAHARASHTRA
400055
India 
Phone  91-22-66499243  
Fax    
Email  rajendras@lifesan.in  
 
Source of Monetary or Material Support  
Sponsor of the study i.e. Centaur Pharmaceuticals Pvt. Ltd. Contact details:912266499100 
 
Primary Sponsor  
Name  Centaur Pharmaceuticals Pvt. Ltd 
Address  Centaur House, Opp. Grand Hyatt, Vakola, Santacruz – East, Mumbai - 400 055, India. 
Type of Sponsor  Pharmaceutical industry-Indian 
 
Details of Secondary Sponsor  
Name  Address 
CRO LifeSan Clinical Research division of Centaur Pharmaceuticals Pvt Ltd  5th and 6th floor, B wing, Centaur House, Opp. Grand Hyatt, Vakola, Santacruz – East, Mumbai - 400 055, India. 
 
Countries of Recruitment     India  
Sites of Study
Modification(s)  
No of Sites = 13  
Name of Principal Investigator  Name of Site  Site Address  Phone/Fax/Email 
Dr Pratik Ajagekar  Accord Hospital  Santnagar,sector no. 4, Moshi Pradhikaran, Pune-Nashik Highway, Pune
Mumbai
MAHARASHTRA 
9067847843

pratik.ajagekar@gmail.com 
Dr S Manwar Ali  All India Institute of Medical Sciences Bhubaneswar   Department of General Medicine, Sijua, post Dumduma, Bhubaneswar
Khordha
ORISSA 
9488820340

alysmanwar@yahoo.co.in 
Dr Aadarsh Kabra   Apex Hospital private Limited  SP 4 and 6, Malviya Industrial Area, Malviya Nagar, Jaipur 302017, Rajasthan India
Jaipur
RAJASTHAN 
9560001846

aadarash61@yahoo.com 
Dr Mrutyunjay Uppin  BHS Lakeview Hospital  R.S. No. 73/7, C.T.S. No. 11888, Gokak Road, 73/7, Opp. Fort Lake, Azad Nagar, Gandhi Nagar, Belagavi, Karnataka 590016
Belgaum
KARNATAKA 
9916260345

jay.45appu@gmail.com 
Dr Gulab Dhar Yadav  GSVM medical college, Kanpur  Department of Surgery, Swaroop Nagar, Kanpur Nagar, Uttar Pradesh - 208002, India
Kanpur Nagar
UTTAR PRADESH 
9415092218

yadavgd71@gmail.com 
Dr Shiv Kumar Bunkar  Jawahar Lal Nehru Medical College  Department of surgery, Kala Bagh, Ajmer-305001, Rajasthan
Ajmer
RAJASTHAN 
9829072396

clinical.jln@gmail.com 
Dr Santanu Dutta  Life Line diagnostic Centre Cum Nursing Home  Consultant Department of CTVS, Life Line Diagnostic Centre cum Nursing Home, 4A, Wood Street, Kolkata- 700016, West Bengal, India
Kolkata
WEST BENGAL 
9830808666

dr.santanudutta14@gmail.com 
Dr Vinoth S  MGM college & research Institute, Pondicherry   NH-45A, Pondy-Cuddalore Main Road, Pillaiyarkuppam, Pondicherry- 607 403
Pondicherry
PONDICHERRY 
9443667373

drvino84@gmail.com 
Dr Binay Krishna Sarkar  N.R.S. Medical College & Hospital  Department of Cardio thoracic & Vascular Surgery, 138 A.J.C. Bose Road, Kolkata-700014
Kolkata
WEST BENGAL 
9433334159

binayskr@hotmail.com 
Dr Sanjay Dakhore  Niramay Hospital, Nagpur  518, Niramay Hospital, Untkhana Medical college road, Nagpur-440009
Nagpur
MAHARASHTRA 
7775052790

sanjaydakhore14@gmail.com 
Dr Sagar Vaghela  Smt. N.H.L Municipal Medical college, SVP institute of Medical sciences and Research  First floor, general medicine, Elis bridge, Ahmedabad - 380006
Ahmadabad
GUJARAT 
9879429996

vaghelasagar@ymail.com 
Dr Aviral Tyagi  Subharti Medical College & Hospital, Swami Vivekanand Subharti University  Subharti Medical college & Hospital, Subhartipuram, NH-58, Delhi- Haridwar Bypass Road, Meerut, Uttar Pradesh-250005
Meerut
UTTAR PRADESH 
9760725396

aviraltyagi@yahoo.com 
Dr Sunil Kumar V   Victoria Hospital, Bangalore Medical College and Research Center [BMCRI]  Department of General Surgery, Victoria Hospital, BMCRI, K R Road Fort Bangalore 560002 - Karnataka
Bangalore
KARNATAKA 
7702692660

sunildocv@gmail.com 
 
Details of Ethics Committee
Modification(s)  
No of Ethics Committees= 14  
Name of Committee  Approval Status 
All India Institute of medical Sciences Bhubaneswar for Dr. S. Manwar Ali  Approved 
Ethics Committee GSVM medical college Kanpur for Dr. Gulab Yadav  Approved 
Ethics Committee of BMCRI for Dr. Sunil Kumar V  Approved 
Institutional Ethics Committe, Apex Hospitals Pvt. Ltd. for Dr. Aadarsh Kabra  Approved 
Institutional Ethics Committee - Jawahar Lal Nehru Medical College for Dr. Shiv Kumar Bunkar  Approved 
Institutional Ethics Committee Nil Ratan Sircar Medical College for Dr. Binoy Krishna Sarkar  Approved 
Institutional Ethics Committee of Accord Hospital for Dr. Pratik Ajagekar  Approved 
Institutional Ethics Committee of Life Line Diagnostic Centre Cum Nursing Home for Dr. Santanu Dutta  Approved 
Institutional Ethics Committee Subharti Medical College & hospital for Dr. Aviral Tyagi  Approved 
Institutional Human Ethics Committee, MGMCRI for Dr. R. Ganesan  Approved 
Institutional Human Ethics Committee, MGMCRI [Notification of PI change from Dr. R. Ganesan to Dr. Vinoth S.]  Approved 
Lakeview Ethics Committee for Dr. Mrutyunjay Uppin  Approved 
Smt. NHL Municipal Medical College - Institutional Ethics Committee for Dr. Sagar Vaghela  Approved 
Viveka Hospitals Ethics Committee for Dr. Sanjay Dakhore  Approved 
 
Regulatory Clearance Status from DCGI  
Status 
Approved/Obtained 
 
Health Condition / Problems Studied  
Health Type  Condition 
Patients  (1) ICD-10 Condition: I873||Chronic venous hypertension (idiopathic),  
 
Intervention / Comparator Agent  
Type  Name  Details 
Comparator Agent  Active-control 0.9% sodium chloride topical solution.  Pack contains two bottles: Bottle A: Sterile Sodium Chloride Solution BP 0.9% w/v (Cutaneous Solution) Bottle B: Sterile Sodium Chloride Solution BP 0.9% w/v (Cutaneous Solution) Composition: Sodium Chloride IP- 0.9% w/v in sterile neutralized aqueous vehicle - q.s. 
Intervention  DiperoxochloricAcid [DPOCL]topical solution brand named as WOXheal   This pack contains two bottles: Bottle A: Diperoxochloric acidconcentrate (each ml of solution contains: Diperoxochloric AcidConcentrate - 1.16 mg) Bottle B: Sterile Sodium ChlorideSolution BP - 0.9% w/v Prior to application bottle A will be mixedwith bottle B and Reconstituted Solution (Bottle A + Bottle B)would be prepared in the bottle B. The dosage of reconstituted solution is 3.5 mL which is apply with help of the dropper on the 5 x 5 cm dressing (inner gauze) to bring the dressing to earth moist conditions. As per size of the dressing, the dose would vary, i.e. for 5 x 10 cm dressing (inner gauze), 7 mL solution would be needed, whereas for 10 x 10 cm dressing (inner gauze), 14 mL solution would be needed. Similarly, for 4 x 4 cm dressing (inner gauze), 2.5 mL solution would be needed. 
 
Inclusion Criteria  
Age From  18.00 Year(s)
Age To  80.00 Year(s)
Gender  Both 
Details  1.Age: > 18 to ≤ 80 years.
2.Gender: Male or female
3.All the patients willing to voluntarily participate in the clinical trial and signing on duly filled Informed Consent Form.
4.Patients with at least one or maximum 3 difficult-to-heal varicose or post-thrombotic venous leg ulcer on standard care (appropriate compression system) localized between the knee and ankle, including the perimalleolar area.
5.Venous insufficiency confirmed by a venous Doppler/ duplex ultrasound scan. A previous scan done within 6 months before randomization can be used. If there is no previous adequate scanning, a new scanning has to be performed before randomization.
6.The target ulcer area should be > 4cm2 and ≤ 40 cm2 calculated by largest length × largest width with less than 50% of necrotic tissue.
7.Ulcer duration ≥2 months and ≤3 years.
8.Target ulcer classification using WIFI grade: Wound 1 or 2, Ischemia 1 or 2, foot infection 1 or 2 
 
ExclusionCriteria 
Details  1.Patients with more than 3 ulcers in a single extremity.
2.Patients with ulcers measuring wound surface area more than 40 cm2 and deep ulcerations with bone or tendon exposure, or uncontrolled infection.
3.Patients who have excessive tissue necrosis that is unlikely to benefit from medication, or those Patients who, in the opinion of the Investigator, are felt likely to require revascularization within 1 month of screening.
4.Patients with severe limb ischemia.
5.Patients who are considered likely to require a major amputation (at or above the ankle) within 3 months of screening.
6.Patients who have had a technically successful revascularization by surgery or angioplasty within 2 months. If Patients are more than two months from revascularization and still meet the inclusion criteria, they may be considered for enrollment. Patients who have had a technically unsuccessful attempt at revascularization may be enrolled at least one week from the procedure.
7.Treatment of foot ulcers with growth factors, stem cells or equivalent medication within the 8 weeks prior to screening.
8.Patients having progressive weight loss, i.e. ≥ 20% within last 3 months prior to enrollment in the trial.
9.Poor nutritional status albumin < 2.5 g/dL or total protein < 4.5 g/dL.
10.Treatment with corticosteroids, immunosuppressive or chemotherapeutic/ cytotoxic agents, radiotherapy – either ongoing or within 60 days prior to enrolment in the trial.
11.Other diseases, which can alter the course of ulcer such as connective tissue disease, renal failure (serum creatinine > 3mg /dL) liver failure, malignancy.
12.Pregnant or nursing mothers.
13.Mentally or neurologically disabled Patients that are considered not fit to approve their participation in the study.
14.Participation in another experimental clinical study during previous 3 months prior to entry in the study. 
 
Method of Generating Random Sequence   Computer generated randomization 
Method of Concealment   Centralized 
Blinding/Masking   Participant and Investigator Blinded 
Primary Outcome  
Outcome  TimePoints 
The primary objective of this study is to evaluate the efficacy of Diperoxochloric acid topical solution in addition to standard of care causing complete wound closure or reduction in wound surface area in the chronic venous leg ulcers in comparison with active control 0.9% sodium chloride topical solution in addition to standard of care on the basis of primary, secondary and tertiary endpoints.  Seven [7] visits are planned in this study:
1.Visit 0 will be the baseline on day 0 before first treatment by Test or Active-control medication, followed by
2.Visit 1: day 7,
3.Visit 2: day 14,
4.Visit 3: day 28,
5.Visit 4: day 42,
6.Visit 5: day 56
7.Visit 6: day 70 and
8.Visit 7: day 84
For each visit, a grace period of ± 3 days will be given to patients and will not be considered as protocol deviation. 
 
Secondary Outcome  
Outcome  TimePoints 
The secondary objective of this study is to evaluate the safety of Diperoxochloric acid topical solution in addition to standard of care in comparison with active control 0.9% sodium chloride topical solution in addition to standard of care by comparing treatment-emergent adverse events or serious adverse events as well as comparison of baseline and end-of-study assessment by virtue of physical examination and clinical laboratory investigations  Seven [7] visits are planned in this study:
1.Visit 0 will be the baseline on day 0 before first treatment by Test or Active-control medication, followed by
2.Visit 1: day 7,
3.Visit 2: day 14,
4.Visit 3: day 28,
5.Visit 4: day 42,
6.Visit 5: day 56
7.Visit 6: day 70 and
8.Visit 7: day 84
For each visit, a grace period of ± 3 days will be given to patients and will not be considered as protocol deviation. 
 
Target Sample Size   Total Sample Size="204"
Sample Size from India="204" 
Final Enrollment numbers achieved (Total)= "229"
Final Enrollment numbers achieved (India)="229" 
Phase of Trial   Phase 3 
Date of First Enrollment (India)   03/07/2023 
Date of Study Completion (India) 06/11/2025 
Date of First Enrollment (Global)  Date Missing 
Date of Study Completion (Global) Date Missing 
Estimated Duration of Trial   Years="1"
Months="6"
Days="0" 
Recruitment Status of Trial (Global)
Modification(s)  
Not Applicable 
Recruitment Status of Trial (India)  Completed 
Publication Details    
Individual Participant Data (IPD) Sharing Statement

Will individual participant data (IPD) be shared publicly (including data dictionaries)?  

Response - NO
Brief Summary   Diperoxochloric acid topical solution is approved by CDSCO in the indication of diabetic neuropathic foot ulcer of skin and subcutaneous tissue. From the data available from phase II and phase III clinical trial, DPOCL caused faster healing of the ulcer when compared to active-control treatment in terms of week wise percentage reduction in wound surface area. It was also observed that certain clinicians successfully used WOXheal [Diperoxochloric acid] topical solution off-label in the treatment of non-healing venous leg ulcers, decubitus ulcers [pressure ulcers] as well as other non-healing ulcers caused by collagen vascular disease.Therefore, this clinical trial is planned to investigate efficacy and safety of WOXheal [Diperoxochloric acid] topical solution in the treatment of venous leg ulcers in addition to standard care of treatment.
Study design:
1. Diperoxochloric acid topical solution is approved by CDSCO in the indication of diabetic neuropathic foot ulcer of skin and subcutaneous tissue7. This clinical trial is planned as part of approval of Diperoxochloric acid topical solution in the additional indication of chronic venous leg ulcers based on this new phase III randomized clinical trial in compliance with New Drug and CT rules, March 19, 2019 [GSR 227E].
2. It will be a prospective, double-blind, multi-centered, randomized, active-control, comparative phase III clinical trial to test the efficacy and safety of Diperoxochloric Acid [DPOCL] topical solution in patients with chronic venous leg ulcers in addition to standard of care vis-à-vis isotonic normal saline solution.
3.The study will be conducted in the hospital setup, with number of patients not less than 204, i.e. at least 102 in the Test arm and 102 in the Active-control arm for a statistically evaluable 84 patients in each arm. The patients could be treated on outpatient [OP] basis or may be in need of in-patient admission [IP]. The sample size estimation is described in section 17.1 of this protocol.

4.A detailed medical history will be obtained from all enrolled Patients, which will be followed by thorough clinical examination.

5. Following the screening procedure as part of this trial, the eligibility of the patient to participate in the trial would be decided by the investigator based on the inclusion-exclusion criteria described in the protocol.

6. Depending on the size of the dressing [5×5 or 5×10 or 10×10 or as per investigator’s discretiondepending on wound surface area], one or two or more units of investigational product will be provided to the Patient at each visit by the investigator at no cost. Since the trial has double-blind study design, the patient or the investigator would not know the identity of investigational product [Test or active-control].

7. Total duration of the clinical trial for any patient who is randomized and enrolled will be maximum of 12 weeks. The trial could end prior to week 12 for an individual patient, if complete wound closure is achieved earlier. On the other hand, the investigator could switch the patient on alternative treatment modality, if there is no satisfactory response observed from the treatment. However, from the enrolment in the study on day 0, the patient must complete at least 4 weeks, to qualify him/ her for the decision of switching to alternative treatment modality due to unsatisfactory treatment response. This will not be considered as adverse event.

8.  Patients will be instructed to prepare the reconstituted solution (ready to use solution) as per the instructions given below.

Open ‘Bottle A’ and ‘Bottle B’.

 Pour the contents of Bottle A in to Bottle B.

 Close the bottle B with bottle cap.

 Mix the content by gently moving the bottle upside down for 5 times.

9.Patients will be asked to use the reconstituted solution within 14 days once it is prepared. Reconstituted solution should not be used after 14 days of mixing/ reconstitution.

10.Patient will be instructed to use the reconstituted solution as per the instructions given below:

Wash hands thoroughly before applying DPOCL topical solution.

Take out the dropper from the plastic bag (provided in the pack) and fill it on the bottle B.

Apply 3.5 mL of this solution with help of the dropper on the 5 x 5 cm dressing (inner gauze) to bring the dressing to earth moist conditions. As per size of the dressing, the dose would vary, i.e. for 5 x 10 cm dressing (inner gauze), 7 mL solution would be needed, whereas for 10 x 10 cm dressing (inner gauze), 14 mL solution would be needed. Similarly, for 4 x 4 cm dressing (inner gauze), 2.5 mL solution would be needed.

 Do not touch dropper to the dressing or gauze.

Then apply this inner gauze on the wound.

 Cover this inner gauze with outer gauze which will prevent the wound area from running dry.

 Fix the gauze in position by rolling the bandage over the gauze and secure the ends by the microtape.

 Daily remove the outer bandage and apply investigational product on inner bandage without removing the inner bandage form the wound.

Change the outer gauze daily and inner gauze on every alternate day.

10. Patients will be instructed to keep a note of daily symptoms and inform the investigator.

11. In case of adverse event/ serious adverse event irrespective of whether it is due to study procedure or study drug and anytime within 12 weeks from the enrolment, the investigator could withdraw the patient and switch to appropriate medical or surgical management of the said AE/ SAE.

12.  All the patients will be asked to revisit the clinical trial center for efficacy and safety assessment on day 7, day 14, day 28, day 42, day 56, day 70 and day 84. For each visit, a grace period of ± 3 days will be given to patients and will not be considered as protocol deviation.

13. If the CWC is achieved prior to scheduled visit, it is acceptable to ask the patient to visit the clinical trial site for evaluation of CWC. Alternatively, if the patient experiences any AE or SAE, he/ she could visit the clinical trial site before the scheduled visit.

14. In case the CWC is achieved prior to scheduled day of visit, the patient could come to the hospital and procedures related to end-of-study [EOS] and post-study safety assessment would be done. This, again would not be considered as protocol deviation.

15. If the wound is not completely healed at the end of the trial (Day 84) then it will be decided by the investigator whether the treatment is needed and should be continued until CWC. In this case the patient would be provided with investigational product after opening the blind.

 
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