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CTRI Number  CTRI/2023/01/049053 [Registered on: 17/01/2023] Trial Registered Prospectively
Last Modified On: 28/07/2025
Post Graduate Thesis  No 
Type of Trial  Interventional 
Type of Study   Drug 
Study Design  Randomized, Parallel Group, Placebo Controlled Trial 
Public Title of Study   Study to Evaluate the Bioequivalence using Clinical Endpoint of Tretinoin Gel Microsphere, 0.06% (Encube Ethicals Private Limited, India)in Subjects with Acne Vulgaris.  
Scientific Title of Study   A Multi-Center, Double-Blind, Randomized, Placebo-Controlled, Three-arm, Parallel Study to Evaluate the Bioequivalence using Clinical Endpoint of Tretinoin Gel Microsphere, 0.06% (Encube Ethicals Private Limi ted, India) to Retin-A Micro® (Tretinoin) Gel Microsphere, 0.06% (Valeant Pharmaceuticals North America LLC, NJ 08807, USA) in Subjects with Acne Vulgaris. 
Trial Acronym  G7SYN/P-001/2022 
Secondary IDs if Any  
Secondary ID  Identifier 
G7SYN/P-001/2022, Version No-01, Dated 06-04-2022   Protocol Number 
 
Details of Principal Investigator or overall Trial Coordinator (multi-center study)  
Name  Dr Ajay Alexander  
Designation  Head-Clinical Trial 
Affiliation  G7 Synergon Private Limited  
Address  No 537 9th Cross 5th main Tata Nagar Sahakaranagar Post Bangalore Karnataka India

Bangalore
KARNATAKA
560092
India 
Phone  9916252529   
Fax    
Email  ajay.alexander@g7synergon.in  
 
Details of Contact Person
Scientific Query
 
Name  Dr D Sathish Kumar  
Designation  Managing Director  
Affiliation  G7 Synergon Private Limited  
Address  No 537 9th Cross 5th main Tata Nagar Sahakaranagar Post Bangalore Karnataka India

Bangalore
KARNATAKA
560092
India 
Phone  9677014651  
Fax    
Email  sathishkumar@g7synergon.in  
 
Details of Contact Person
Public Query
 
Name  Dr D Sathish Kumar  
Designation  Managing Director  
Affiliation  G7 Synergon Private Limited  
Address  No 537 9th Cross 5th main Tata Nagar Sahakaranagar Post Bangalore Karnataka India

Bangalore
KARNATAKA
560092
India 
Phone  9677014651  
Fax    
Email  sathishkumar@g7synergon.in  
 
Source of Monetary or Material Support  
Encube Ethicals Private Limited 
 
Primary Sponsor  
Name  Encube Ethicals Private Limited 
Address  Encube Ethicals Private Limited Unit No. 24, Steelmade Industrial Estate, Marol Village, Andheri (E), Mumbai – 400 059, Maharashtra, India  
Type of Sponsor  Pharmaceutical industry-Indian 
 
Details of Secondary Sponsor  
Name  Address 
NIL  NIL 
 
Countries of Recruitment     India  
Sites of Study
Modification(s)  
No of Sites = 21  
Name of Principal Investigator  Name of Site  Site Address  Phone/Fax/Email 
Dr Dilip Gopal Jogaikar  7 Orange Hospital  2nd & 3rd Floor, Research Department, 7- Orange Hospital, Pawana Nagar, Next to Jain, School, Near Chapekar Chowk, Chinchwad, Pune-411033, Maharashtra, India
Pune
MAHARASHTRA 
8983377103

drdjogaikar@gmail.com 
Dr Bhushan Sevakram Madke  Acharya Vinoba Bhave rural hospital  Ground floor, Clinical research department, Acharya Vinoba Bhave rural hospital, Sawangi (Meghe), Wardha-442004, Maharashtra, India
Wardha
MAHARASHTRA 
9340885393

drbhushan81@gmail.com 
Dr Vipul Gupta  Atharva Hospital  Basement, Clinical Research Department, Atharva Multispeciality Hospital and Research Centre, H-4, Comm-2, Construction Div - 21, UP Avas Vikas Parishad, Sector - E, Lucknow - 226002, UP, India
Lucknow
UTTAR PRADESH 
9005044010

dr.vipulgupta24@gmail.com 
Dr Renuka Ashtekar  Bharati Vidyapeeth (Deemed to be University) Medical College and Hospital  Ground floor, Clinical research department, Bharati Vidyapeeth (Deemed to be University) Medical College and Hospital Sangli, Sangli - Miraj Rd, Wanlesswadi, Sangli - 416416, Maharashtra, India
Sangli
MAHARASHTRA 
9921653812

renukaashtekar75@gmail.com 
Dr Nisha Parikh  CIMET’S Inamdar Multispeciality Hospital  Basement, Clinical Research Department, CIMET’S Inamdar multispeciality hospital, S No 15, Fatimanagar, Wanawadi, Pune-411040, Maharashtra, India
Pune
MAHARASHTRA 
9673834553

drnishaparikh.research@gmail.com 
Dr Nikam Vivek Vilas  Dr Vasant Rao Pawar Medical college and Hospital   Room no. I-239, I-240, First floor, Clinical trial unit, Dr. Vasantrao Pawar Medical College, Hospital & Research Centre, Vasantdada Nagar, Adgaon, Nashik- 422003, Maharashtra, India
Nashik
MAHARASHTRA 
9638888527

viveknikam555@gmail.com 
Dr Sakshi Midha  Excelcare Hospital  Room No.301, Research department, third floor, Excelcare Hospital, 103, Sanjay Nagar A, Joshi Marg, Kalwar Road, Jaipur- 302012, Rajasthan, India
Jaipur
RAJASTHAN 
9001711113
9828039960
sakshi.midha@gmail.com 
Dr Vishal Laxmanrao Gore Patil  Global Hospital  Ground Floor, Research Department, Global Hospital & Research Institute, Off Sinhgad Road, Near Dattawadi Police, Chowki, Dattawadi, Pune - 411030, Maharashtra, India
Pune
MAHARASHTRA 
9657746968

drvishalgore999@gmail.com 
Dr Bangaru H  K R Hospital attached to Mysore medical college and research institute  First Floor, Department of Dermatology, K R Hospital attached to Mysore medical college and research institute, Irwin road, Mysuru-570001, Karnataka, India
Mysore
KARNATAKA 
9886789231

drbangaruskin@gmail.com 
Dr Patnala Guru Prasad  King George Hospital  First Floor, Clinical research area, department of dermatology, King George Hospital Maharanipeta, Visakpatanam-530002, Andhra Pradesh, India
Visakhapatnam
ANDHRA PRADESH 
9848022615

drpguruprasadresearch@gmail.com 
Dr Shivakumar Patil  KLE’s Dr Prabhakar Kore hospital and Medical research Centre  Second Floor, Site Management office, KLE’s Dr Prabhakar Kore hospital and Medical research Centre, Nehru nagar, Belagavi-590010,
Belgaum
KARNATAKA 
9844512315

shivakumarkpatil@gmail.com 
Dr Sonal Mahadev Shendkar  Lifepoint Multispecialty Hospital  3rd Floor, Lifepoint Multispecialty Hospital, 145/1, Mumbai Bangalore highway, Near hotel Sayaji, Wakad, Pune – 411057, Maharashtra India
Pune
MAHARASHTRA 
9960178611

shendkar.sonal82@gmail.com 
Dr Grandhi Sudhakarrao Venkata  Medipoint Hospitals Private Limited  Third floor, research department, clinic building, Medipoint hospitals Private limited, 241/1, New D. P.Road, Aundh, Pune-411007, Maharashtra, India
Pune
MAHARASHTRA 
9850082614

sudhakargrandhi.pentagon@gmail.com 
Dr Mamta Patil  Ojas Multispeciality Hospital  Clinical Research Room, Ground floor, Ojas Multispeciality Hospital,Sr.no 230/1, D.Y. Patil College Road, Ravet, Pune-412101, Maharashtra , India
Pune
MAHARASHTRA 
7738927929

mamata.ojas@gmail.com 
Dr Makwana Miss Dhwaniben Ranchhodbhai  Parth Hospital  Room no.401 & 402, Research Department, E/405-4, 407-411, Fourth floor Galaxy arcade, near Galaxy cinema, Naroda-382330, Gujarat, India
Ahmadabad
GUJARAT 
9662333253

dhwanimakwanacr722@gmail.com 
Dr Pranali Rathod  Saideep Healthcare and Research Private limited  Fourth Floor, Clinical Research Department, Saideep Healthcare and research Private limited, Viraj Estate, behind Yashwant colony, Near DSP chowk, Ahmednagar-414003, Maharashtra, India
Ahmadnagar
MAHARASHTRA 
7721929058

pranalirathod725@gmail.com 
Dr Sudhir Singh  Shalinitai Meghe Hospital and Research Centre  Ground floor, Clinical research room, building C, Shalinitai Meghe Hospital and research centre, Wanadongri, Hingna road, Nagpur-441110, Maharashtra, India
Nagpur
MAHARASHTRA 
8806187862

sudhirderma@gmail.com 
Dr Chetan Lalseta  Shree Giriraj Multispeciality Hospital  First Floor, CRC Department, A unit of Giriraj Lifecare Private Limited, 27- Navjyot park, 150ft Ring road, Rajkot- 360005, Gujarat, India
Rajkot
GUJARAT 
9825199585

chetanlalseta@gmail.com 
Dr Rashmi Singh  Shubham Sudbhawana Superspeciality Hospital  Third Floor, Clinical Research Department, Shubham Sudbhawana Superspeciality Hospital B 31/80, 23-B, Bhogabeer, Lanka, Varanasi-221005, Uttar Pradesh, India
Varanasi
UTTAR PRADESH 
7080063652

sweetrashmi4364@gmail.com 
Dr Subhash Bharti  Vedant Multispecialty Hospital  Clinical Research Department , Cabin no. 1 and 2, Basement Floor, Vedant Multispecialty Hospital, GP 83,Sambhaji Nagar, MIDC, Chinchwad, Pune- 411019, Maharashtra, India
Pune
MAHARASHTRA 
9999883007

drsubhashbharti.vedant@gmail.com 
Dr Parag Kalyani  Villoo Poonawala Hospital  Room no:13, Basement, Clinical Research Department, Villoo Poonawala Memorial Hospital, Pune - Solapur Road, Near Savli, Corner, Hadapsar, Pune - 411028, Maharashtra, India
Pune
MAHARASHTRA 
9130603303

parag.kalyani@yahoo.com 
 
Details of Ethics Committee
Modification(s)  
No of Ethics Committees= 21  
Name of Committee  Approval Status 
Ethics Committee Inamdar Multispeciality Hospital  Approved 
IEC – Sai Sneh Hospital and Diagnostic Centre (7 Orange Hospital)  Approved 
IEC – Sai Sneh Hospital and Diagnostic Centre (Global Hospital)  Approved 
IEC – WMFs Villoo Poonawala Memorial Hospital  Approved 
IEC for atharva multispeciality hosp. and research centre  Approved 
IEC King George hospital   Approved 
IEC, Dr Vasant Rao Pawar Medical college, Nashik  Approved 
IEC- MMC and RI and associated hospital  Approved 
IEC-Vedant Multispeciality Hospital   Approved 
Institutional Ethics Commitee of DMIHER  Approved 
Institutional Ethics Committee  Approved 
Institutional Ethics Committee BVDU Medical College and Hospital, Sangli   Approved 
Institutional Ethics Committee, KLE University   Approved 
Institutional Ethics Committee, Saideep Hospital  Approved 
Lifepoint Research- Ethics Committee  Approved 
Office of Institute of Ethics Committee  Approved 
Ojas Multispeciality Hospital Ethics Committee   Approved 
Parth Hospital Ethics Committee  Approved 
Penta-Med Ethics Committee   Approved 
Shree Giriraj Hospital Research Ethics Committee  Approved 
Shubham Sudbhawana Super. Hosp. Ethics Committee  Approved 
 
Regulatory Clearance Status from DCGI
Modification(s)  
Status 
Approved/Obtained 
 
Health Condition / Problems Studied  
Health Type  Condition 
Patients  (1) ICD-10 Condition: L700||Acne vulgaris,  
 
Intervention / Comparator Agent  
Type  Name  Details 
Comparator Agent  Gel vehicle of test product manufactured by Encube Ethicals Private Limited, India.  Subjects will be instructed to apply the Investigational Product once a day. Subjects will cleanse the face with a mild or soapless, non-medicated cleanser, dry skin gently, wait for 20 to 30 minutes before applying the study medication, and then apply enough product to lightly cover the entire affected areas of the face once daily at bedtime for 84 consecutive days. 
Comparator Agent  Retin-A Micro® (Tretinoin) Gel Microsphere 0.06% manufactured by Valeant Pharmaceuticals International, Inc. Laval, Quebec H7L 4A8, Canada and manufactured for Valeant Pharmaceuticals North America LLC, Bridgewater, NJ 08807, USA.   Subjects will be instructed to apply the Investigational Product once a day. Subjects will cleanse the face with a mild or soapless, non-medicated cleanser, dry skin gently, wait for 20 to 30 minutes before applying the study medication, and then apply enough product to lightly cover the entire affected areas of the face once daily at bedtime for 84 consecutive days.  
Intervention  Tretinoin Gel Microsphere, 0.06% manufactured by Encube Ethicals Private Limited, India.  Subjects will be instructed to apply the Investigational Product once a day. Subjects will cleanse the face with a mild or soapless, non-medicated cleanser, dry skin gently, wait for 20 to 30 minutes before applying the study medication, and then apply enough product to lightly cover the entire affected areas of the face once daily at bedtime for 84 consecutive days.  
 
Inclusion Criteria  
Age From  12.00 Year(s)
Age To  40.00 Year(s)
Gender  Both 
Details  1. Healthy male or non-pregnant, non-lactating female aged ≥ 12 and ≤ 40 years with a clinical diagnosis of Acne vulgaris (AV).
2. On the face, having ≥ 20 inflammatory (i.e., papules and pustules), ≥ 25 non-inflammatory (i.e., open and closed comedones) lesions and ≤ 2 nodulocystic lesions (i.e, nodules and cysts).
3. Investigator’s Global Assessment (IGA) of acne severity grade 2, 3 or 4.
4. Willing to refrain from use of all other topical products (moisturizer, new brands of make-up, creams, lotions, powders or any other topical product), all acne medications and antibiotics during the 12 weeks treatment period.
5. Female subjects of child bearing potential practicing an approved method of contraception and willing to continue its use from study entry to 7 days after completion of the last administration of Investigational Product and have a negative Urine Pregnancy Test at the time of screening
or
Female subjects of non-child bearing potential.
Note: Approved methods of contraception include Hormonal contraception including oral, systemic injectable or Intra-uterine Contraceptive Devices (subject must have been on a stable dose for 3 months prior to study entry), bilateral tubal ligation or tubectomy, non-hormonal IUCD, double barrier or strict abstinence.
Use of oral contraceptive therapy is allowed if it shall remain constant throughout the study. Non-child bearing potential is defined as pre-menarche, postmenopausal absence of menstrual bleeding for 1 year prior to enrolment, hysterectomy or bilateral oophorectomy.

6. Male subjects must use accepted methods of birth control or must agree to practice abstinence from study entry to 7 days after the completion of last administration of Investigational Product.
7. Willing to provide written informed consent or assent, as applicable. For subjects who are considered minors (< 18 completed years), the parent or legal guardian shall sign the consent form and the child shall be required to sign a subject “assent” form, as appropriate.
 
 
ExclusionCriteria 
Details  1. Presence of any skin condition that would interfere with the diagnosis or assessment of Acne Vulgaris (e.g., on the face: rosacea, dermatitis, psoriasis, squamous cell carcinoma, eczema, acneform eruptions caused by medications, steroid acne, steroid folliculitis, or bacterial folliculitis).
2. Excessive facial hair (e.g. beards, sideburns, moustaches, etc.) that shall interfere with diagnosis or assessment of Acne Vulgaris. Well-trimmed moustaches are allowed.
3. History of hypersensitivity or allergy to tretinoin, retinoids and/or any of the study medication ingredients.
4. Use within 6 months prior to baseline (Randomisation) of oral retinoids (e.g. Accutane®) or therapeutic vitamin A supplements of greater than 10,000 units/day (multivitamins are allowed).
5. Use for less than 3 months prior to baseline (Randomisation) of estrogens or oral contraceptives or any other hormonal therapy; use of such therapy is allowed if it shall remain constant throughout the study.
6. Use on the face within 1 month prior to baseline (Randomisation) cryo-destruction or chemo-destruction, dermabrasion / microdermabrasion, photodynamic therapy, acne surgery, intralesional steroids, X-ray therapy, chemical or laser peel.
7. Use within 1 month prior to baseline (Randomisation) of androgen receptor blockers for acne (spironolactone, Flutamide etc.,), systemic steroids (Including intra-nasal and in-haled corticosteroids), systemic antibiotics, systemic treatment for Acne Vulgaris (other than oral retinoids, which require a 6-month washout) or systemic anti-inflammatory agents.
8. Use within 2 weeks prior to baseline (Randomisation) of topical steroids, topical retinoids and topical acne treatments including over-the-counter preparations, topical anti-inflammatory agents, medicated cleansers/shampoo or topical antibiotics.
9. Use within 2 weeks prior to baseline (Randomisation) of abradants, facials, peels containing glycolic or other acids, masks, washes or soaps, containing glycolic acid, salicylic acid, Alpha- or beta-hydroxy acids or other acids, benzoyl peroxide (BPO) or sulfacetamide sodium, non-mild facial cleansers, moisturizers that contained retinol.
10. Subjects who have undergone a facial procedure (e.g., laser peel, microdermabrasion or blue light treatment, etc.) within the past 4 weeks or if it is planned to be performed during the conduct of the study.
11. Concomitant use/planned to use of mega-doses of certain vitamins (such as mega-doses of vitamin D [> 2000 IU/day], vitamin B6 [> 2 mg] or vitamin B12 [> 1 mg/day]), haloperidol, halogens such as iodide and bromide, lithium, hydantoin and phenobarbital.
12. Use of tanning booths or tanning lamps or ultraviolet light within 1 week prior to Baseline and an unwillingness to refrain from use during the study.
13. Subjects with planned unprotected and intense UV exposure during the study (mountain sports, UV radiation, sunbathing, etc.).
14. A significant medical history of or are currently immunocompromised or receiving immunomodulators/biologics since last 3 months.
15. Subjects with clinically significant vital sign abnormality.
16. Subjects with clinically significant unstable medical disorders, life-threatening disease, or current malignancies.
17. Subjects who engage in activities that involve excessive or prolonged exposure to sunlight or weather extremes, such as wind or cold.
18. History of drug or alcohol abuse within last 6 months.
19. Lived in the same household as currently enrolled subjects.
20. Female subjects who are breast-feeding or planning to become pregnant.
21. Subjects who have been treated with an investigational drug or investigational device within a period of 30 days prior to study enrolment.
22. Subjects with a clinically significant disease(s) or disorder(s) other than facial Acne Vulgaris that in the opinion of the Investigator may (i) put the subject at risk because of participation in the study (ii) interfere with the study evaluations or (iii) cause concern regarding subject’s ability to participate in the study.
 
 
Method of Generating Random Sequence   Computer generated randomization 
Method of Concealment   Centralized 
Blinding/Masking   Participant and Investigator Blinded 
Primary Outcome  
Outcome  TimePoints 
Mean percent change from baseline to week 12 in the inflammatory (papules and pustules) lesion count
Mean percent change from baseline to week 12 in the Non-inflammatory (open and closed comedones) lesion count.
 
Baseline to week 12
Baseline to week 12 
 
Secondary Outcome  
Outcome  TimePoints 
Proportion of subjects with a clinical response of “success” at week 12.  week 12 (Day 84)
 
 
Target Sample Size   Total Sample Size="981"
Sample Size from India="981" 
Final Enrollment numbers achieved (Total)= "0"
Final Enrollment numbers achieved (India)="0" 
Phase of Trial   Phase 1 
Date of First Enrollment (India)   01/03/2023 
Date of Study Completion (India) Date Missing 
Date of First Enrollment (Global)  Date Missing 
Date of Study Completion (Global) Date Missing 
Estimated Duration of Trial   Years="1"
Months="0"
Days="0" 
Recruitment Status of Trial (Global)
Modification(s)  
Not Applicable 
Recruitment Status of Trial (India)  Completed 
Publication Details   Nil 
Individual Participant Data (IPD) Sharing Statement

Will individual participant data (IPD) be shared publicly (including data dictionaries)?  

Response - NO
Brief Summary
Modification(s)  
A Multi-Center, Double-Blind, Randomized, Placebo-Controlled, Three-arm, Parallel Study to Evaluate the Bioequivalence using Clinical Endpoint of Tretinoin Gel microsphere, 0.06% in the Treatment of 981 Subjects with Subjects with Acne VulgarisAt screening/baseline visit, subjects shall be randomly (Double’blind) assigned in 1:1:1 fashion to one of the 3 treatment groups at visit 1.  Statistical comparisons shall be made between test and reference, test and placebo, and reference and placebo. The primary endpoint is test/reference ratio of the mean percent change from baseline to week 12 in the inflammatory (papules and pustules) and non- inflammatory (Comedones) lesion count at Visit 5. The secondary endpoint is the proportion of subjects who are considered a Clinical Success at Week 12. Safety shall be evaluated from reported adverse events and application site reaction assessments. 
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