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CTRI Number  CTRI/2022/12/048435 [Registered on: 23/12/2022] Trial Registered Prospectively
Last Modified On: 27/06/2023
Post Graduate Thesis  No 
Type of Trial  BA/BE 
Type of Study    
Study Design  Randomized, Crossover Trial 
Public Title of Study   A Bioequivalence study of Dapagliflozin 10mg and Gliclazide 60mg Sustained release tablets on healthy human volunteers to compare the plasma concentrations between test product and marketed product. 
Scientific Title of Study   An open label, randomized, balanced, two treatment, two sequence, two period, cross-over, single-dose, oral bioequivalence study of Dapagliflozin 10mg and Gliclazide Sustained Release tablets 60 mg (T) Manufactured by Eris Lifesciences Ltd, India with Forxiga® 10mg (Dapagliflozin 10mg Tablets) (R1) Marketed by AstraZeneca Pharma India Ltd., India and Diamicron® XR 60mg (Gliclazide extended release tablet) (R2) Manufactured by Serdia Pharmaceuticals (India) Pvt, Ltd., India in normal healthy, adult human subjects under fasting condition. 
Trial Acronym   
Secondary IDs if Any  
Secondary ID  Identifier 
S-22-734 Version 01 Dated- 13-Sep-2022  Protocol Number 
 
Details of Principal Investigator or overall Trial Coordinator (multi-center study)  
Name  Dr Harisha C 
Designation  MBBS, Clinical Investigator 
Affiliation  Notrox Research Pvt Ltd 
Address  No 19-3,2nd Floor, Bikasipura road, JC Industrial layout, off Kanakapura Road (Behind Metro Cash & carry)

Bangalore
KARNATAKA
560062
India 
Phone    
Fax    
Email  harisha-c@notroxresearch.com  
 
Details of Contact Person
Scientific Query
 
Name  Ganesh Boddu 
Designation  Head- Regulatory Affairs and Clinical Research 
Affiliation  Eris Lifesciences Ltd. 
Address  Eris Lifescience Ltd, Shivarth Ambit, Ramdas Road Off SBR Near Swati Bungalows, Bodakdev, Ahmedabad, Gujarat

Bangalore
KARNATAKA
380054
India 
Phone    
Fax    
Email  Ganesh.boddu@erislifesciences.com  
 
Details of Contact Person
Public Query
 
Name  Ganesh Boddu 
Designation  Head- Regulatory Affairs and Clinical Research 
Affiliation  Eris Lifesciences Ltd. 
Address  Eris Lifescience Ltd, Shivarth Ambit, Ramdas Road Off SBR Near Swati Bungalows, Bodakdev, Ahmedabad, Gujarat

Bangalore
KARNATAKA
380054
India 
Phone    
Fax    
Email  Ganesh.boddu@erislifesciences.com  
 
Source of Monetary or Material Support  
Eris Lifesciences Ltd, Shivarth Ambit, Ramdas Road Off SBR Near Swati Bungalows, Bodakdev Ahmedabad Gujarat 380054 India 
 
Primary Sponsor  
Name  Eris Lifesciences Ltd.  
Address  Eris Lifescience Ltd., Brahmaputra Industrial Park, Village-Silla, Amingaon, North Guwahati, Assam-781031. 
Type of Sponsor  Pharmaceutical industry-Indian 
 
Details of Secondary Sponsor  
Name  Address 
NIL  NIL 
 
Countries of Recruitment     India  
Sites of Study  
No of Sites = 1  
Name of Principal Investigator  Name of Site  Site Address  Phone/Fax/Email 
Dr Harisha C  Notrox Research Pvt Ltd  No 19/3,2nd Floor, Bikasipura road, JC Industrial layout, off Kanakapura Road (Behind Metro Cash & carry) Bangalore -560062
Bangalore
KARNATAKA 
9845933077

harisha-c@notroxresearch.com 
 
Details of Ethics Committee  
No of Ethics Committees= 1  
Name of Committee  Approval Status 
Sri Durgamba Independent ethics committee  Approved 
 
Regulatory Clearance Status from DCGI  
Status 
Approved/Obtained 
 
Health Condition / Problems Studied  
Health Type  Condition 
Healthy Human Volunteers  healthy adult human subjects 
 
Intervention / Comparator Agent  
Type  Name  Details 
Intervention  Dapagliflozin 10mg and Gliclazide Sustained Release tablets 60 mg.  After an overnight fasting of at least 10.00 hours, a single Dapagliflozin 10mg and Gliclazide Sustained Release tablets 60 mg (T) Manufactured by Eris Lifesciences Ltd, India along with 240±2 mL of 20% aqueous glucose solution, will be administered orally to the subjects in sitting posture at ambient temperature in the morning, as per the randomization schedule. Subjects will receive the alternate ‘treatment’ in the subsequent periods, in such a way that each subject will have received all the ‘treatments’ by the end of the study. 
Comparator Agent  Forxiga® 10mg (Dapagliflozin 10mg Tablets) (R1) Marketed by: AstraZeneca Pharma India Ltd., India. Diamicron® XR 60mg (Gliclazide extended release tablet) (R2) Manufactured by: Serdia Pharmaceuticals (India) Pvt, Ltd., India.  After an overnight fasting of at least 10.00 hours, a single Forxiga® 10mg (Dapagliflozin 10mg Tablets) (R1) Marketed by AstraZeneca Pharma India Ltd., India and Diamicron® XR 60mg (Gliclazide extended release tablet) (R2) Manufactured by Serdia Pharmaceuticals (India) Pvt, Ltd., India along with 240±2 mL of 20% aqueous glucose solution, will be administered orally to the subjects in sitting posture at ambient temperature in the morning, as per the randomization schedule. Subjects will receive the alternate ‘treatment’ in the subsequent periods, in such a way that each subject will have received all the ‘treatments’ by the end of the study. 
 
Inclusion Criteria  
Age From  18.00 Year(s)
Age To  45.00 Year(s)
Gender  Both 
Details  Volunteers who accept for participating in this study must:
1. Healthy, adult human, subjects aged between 18-45 years (both inclusive) weighing at least 50 kg at the time of screening.
2. Having a Body Mass Index (BMI) between 18.50 to 30.00 kg/m2 (both inclusive) at the time of screening.
3. Normal or clinically insignificant findings during screening, medical history, medical examination, laboratory evaluations, 12 lead ECG and X-ray chest (posterior-anterior view) recordings.
4. Able to comply with the study procedures, in the opinion of the principal investigator.
5. Compliance with study specific restrictions and prohibitions.
6. Able to give voluntary written informed consent for participation in the trial.
7. Preferably non smoker and non alcoholic.
In case of Female subjects:
8. Female subjects who are of child bearing potential and are surgically sterilized at least 6 months prior to study participation.
9. Female subjects who are of childbearing potential and are willing to use a suitable and effective double barrier contraceptive method or non-hormonal intra uterine device during the study.
10. Female subjects who are tested negative for serum pregnancy test at the time of check-in.
11. Female subjects who are tested negative for urine pregnancy test at the time of screening.
12. Female subjects who has surgically sterilized partner (for at least 6 months).
 
 
ExclusionCriteria 
Details  If any subject is having any of the following conditions, then exclude him/her from participation in this study:
1. Known hypersensitivity or idiosyncratic reaction to the study drug or any related drug.
2. History or presence of any disease or disorder known to influence bone metabolism, compromise the hemopoietin, renal, hepatic, endocrine, pulmonary, central nervous, cardiovascular, immunological, dermatological, gastrointestinal, musculoskeletal or any other body system.
3. Systolic blood pressure less than 90 mmHg or more than 140 mmHg at the time of screening. Minor deviations (2-4 mmHg) may be acceptable at the discretion of the physician/investigator.
4. Diastolic blood pressure less than 60 mmHg or more than 90 mmHg at the time of screening. Minor deviations (2-4 mmHg) may be acceptable at the discretion of the physician/investigator.
5. Pulse rate below 60 beats/minute or above 100 beats/minute at the time of screening.
6. Respiratory rate below 12 or above 20 breaths per minute at the time of screening.
7. Body temperature below 96.2o F or above 99.8 o F at the time of screening.
8. SpO2 less than 94% @ RA at the time of screening.
9. Ingestion of any medicine at any time within 14 days prior to IP administration
10. in period I. In any such case subject selection will be at the discretion of the principal investigator.
11. Habit of consuming high caffeine (more than 5 cups of coffee or tea/day).
12. Smokers who smoke >9 cigarettes per day.
13. Alcoholic who consumes >21units of alcohol in a week.
14. History of dehydration from diarrhea, vomiting or any other reason within a period of 24.00 hours prior to study check-in.
15. An unusual or abnormal diet within 48.00 hours prior to study check-in, whatever reason e.g. because of fasting due to religious reasons.
16. The presence of clinically significant abnormal laboratory values during screening.
17. Use of any recreational drugs or history of drug addiction or testing positive in pre-study urine drug screening and Urine alcohol test.
18. A history of difficulty with donating blood or having donated blood in the preceding 90 days prior to the start of the study.
19. Subject who has participated in any other clinical study involving drug administration and collection of blood samples in the 90 days preceding the start of the study.
20. Difficulty in swallowing capsule/tablet.
21. Positive HIV, VDRL/RPR, Hepatitis B and C tests.
22. Subjects who have used any drugs or substances known to be strong inhibitors or inducers of Cytochrome P450 enzymes within 14 days prior to IP administration in period I.
23. History of undiagnosed vaginal bleeding (for females only).
24. Female subjects who demonstrates a positive pregnancy during screening or currently breast-feeding.
25. Female volunteer who has used implanted or injected hormonal contraceptives anytime during the 6 months prior to study or used hormonal contraceptives within 14 days before dosing.
 
 
Method of Generating Random Sequence   Computer generated randomization 
Method of Concealment   Centralized 
Blinding/Masking   Open Label 
Primary Outcome  
Outcome  TimePoints 
Cmax, AUC(0-t) and AUC(0-inf)  Total 29 blood samples in each period, a single pre-dose (-02.00 to 00.00) blood sample of 4.0 mL will be collected in each period.

The post-dose blood samples of 4.0 mL will be collected at 00.33, 00.67, 01.00, 01.33, 01.67, 02.00, 02.33, 02.67, 03.00, 03.50, 04.00, 04.50, 05.00, 05.50, 06.00, 06.50,07.00, 07.50, 08.00, 09.00, 10.00, 12.00, 16.00, 24.00, 36.00, 48.00, 72.00 and 96.00 hours post-dose.

36.00, 48.00, 72.00 and 96.00 hours ambulatory visit. (window period as ± 60 minutes). 
 
Secondary Outcome  
Outcome  TimePoints 
Tmax, Kel, t½ and AUC% Extrap obs(%)  Total 29 blood samples in each period, a single pre-dose (-02.00 to 00.00) blood sample of 4.0 mL will be collected in each period.

The post-dose blood samples of 4.0 mL will be collected at 00.33, 00.67, 01.00, 01.33, 01.67, 02.00, 02.33, 02.67, 03.00, 03.50, 04.00, 04.50, 05.00, 05.50, 06.00, 06.50,07.00, 07.50, 08.00, 09.00, 10.00, 12.00, 16.00, 24.00, 36.00, 48.00, 72.00 and 96.00 hours post-dose.

36.00, 48.00, 72.00 and 96.00 hours ambulatory visit. (window period as ± 60 minutes). 
 
Target Sample Size   Total Sample Size="24"
Sample Size from India="24" 
Final Enrollment numbers achieved (Total)= "0"
Final Enrollment numbers achieved (India)="0" 
Phase of Trial   Phase 2/ Phase 3 
Date of First Enrollment (India)
Modification(s)  
26/12/2022 
Date of Study Completion (India) Date Missing 
Date of First Enrollment (Global)  Date Missing 
Date of Study Completion (Global) Date Missing 
Estimated Duration of Trial   Years="1"
Months="0"
Days="0" 
Recruitment Status of Trial (Global)
Modification(s)  
Not Applicable 
Recruitment Status of Trial (India)  Completed 
Publication Details   Not yet planned 
Individual Participant Data (IPD) Sharing Statement

Will individual participant data (IPD) be shared publicly (including data dictionaries)?  

Response - NO
Brief Summary   An open label, randomized, balanced, two treatment, two sequence, two period, cross-over, single-dose, oral bioequivalence study of Dapagliflozin 10mg and Gliclazide Sustained Release tablets 60 mg (T) Manufactured by Eris Lifesciences Ltd, India with Forxiga® 10mg (Dapagliflozin 10mg Tablets) (R1) Marketed by AstraZeneca Pharma India Ltd., India and Diamicron® XR 60mg (Gliclazide extended release tablet) (R2) Manufactured by Serdia Pharmaceuticals (India) Pvt, Ltd., India in normal healthy, adult human subjects under fasting condition. 
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