Post-kala-azar dermal Leishmaniasis (PKDL) is a complication of Kala-Azar (KA), characterized by macular, maculopapular, and nodular rashes in a patient who has recovered from KA. In the Indian subcontinent, PKDL is reported in 10–15% of patients treated for KA, usually after an interval of a few months to years. Diagnosis of KA and PKDL by microscopy needs an invasive/risky sample (bone marrow aspirate, splenic aspirate, tissue biopsy), culture test is tedious and time consuming with low sensitivity while RT-PCR is costly, and therefore these tests are not applicable at Community Health Centre (CHC) levels. Immunologic strip test (rK39 test) is inconclusive for KA relapse and PKDL cases due to presence of antibodies after KA. Also, immunological rK39 strip tests cannot be used for assessment of cure. Loop-mediated isothermal amplification (LAMP) assay is a novel molecular diagnostic method to amplify DNA with rapidity and high specificity under isothermal conditions. The LAMP assay is at the forefront in the search for innovative diagnostics for rapid and specific amplification of target DNA under isothermal conditions. Shorter reaction time with visual judgment of positivity without requiring sophisticated equipment makes it an attractive diagnostic method for field application. This will be the first Indian LAMP assay kit for the diagnosis of KA and PKDL. There is a dire need of such a ‘Make in India’ molecular diagnostic kit for the success of KA elimination program. Leish-LAMP detection kit may be used in India, Nepal and Bangladesh as the same L. donovani parasite is responsible for Leishmaniasis in these countries. Leish-LAMP assay can be performed with finger prick blood sample on filter paper, also known as Dried blood spot (DBS).