Endodontic therapy is a debridement procedure that requires complete removal of microorganisms and dead tissue from the root canal and periapical tissue. Irrigation has been used along with mechanical instrumentation to achieve better debridement. Over the decade, the use of herbal drugs as intracanal medicament has increased. One such immensely valuable plant regarding its constituent and pharmacology is Tinospora cordifolia of the Menispermaceae family, commonly called Guduchi in Sanskrit. To overcome the adverse effects of present chemical root canal irrigant, a shift to herbal agents is being studied. Tinospora cordifolia is one among the herbal agents that can be used as an intracanal irrigant because of its antimicrobial properties.

The extract will be prepared in two concentrations 2 percent and 3 percent, other comparative agents Sodium Hypochlorite 3 percent, Chlorhexidine Gluconate 2 percent and Normal Saline will be commercially procured. Patients will be selected depending on the inclusion and exclusion criteria. After sample selection, the tooth under the study will be anesthetise using 2 percent lignocaine and a straight-line access to the canals will be prepared. A first set of root canal samples will be collected using a sterile paper point. For standardization, the samples will be collected from the palatal root in the maxillary arch and distal root in the mandibular arch. After collection of first set of samples, the canals will be irrigated with the respective irrigants along with the cleaning and shaping of the canals and second set of samples will be collected after using the irrigants. The patient is recalled after 3 days and a third set of samples are collected and the root canals are obturated. The collected samples will be stored in a vial containing normal saline and is sent for microbial assessment of gram positive and gram negative, aerobic and anaerobic microorganisms.