All the patients will be selected from the out patients section of The Department of Periodontology, KVG Dental College and Hospital, Sullia. Fifteen Subjects diagnosed with periodontitis undergoing periodontal flap surgeries will be selected for the study.Sextants are selected randomly and divided into two part . Each part is selected randomly to cover with  Nanocrystalline silver membrane under Barricaid® and Coe-pak^TM   dressing materials. clinical parameters will be recorded for each patient after 7 days of surgery and  circum-gingival plaque sample will be obtained from gingival sulcus with the help of curette and kept in Tris EDTA buffer which can be stored for 72 hours at the temperature of 4 ºC  .Those samples will be assessed for Porphyromonas gingivalis count using real time PCR. Following parameters are measured 

         (1)Inflammation papillary marginal attachment index(Maury Massler and Schour) 

        (2)Quigley hein plaque index

 (3) Healing index by Landry et al.

Statistical analysis will be performed to compare the changes of clinical parameter between Barricaid® and   Coe-pak^TM   dressing materials when placed over Nanocrystalline silver membrane.

Prior to the surgical operation, all participants will have an examination and treatment planning appointment, and will receive

suitable oral hygiene instructions as well as an initial scaling and root planing treatment. Flap surgery will be carried out under standard protocol. 

Sextant will be selected and divided into two parts. Each part will be selected randomly to cover with Barricaid®  or Coe-pak^TM   along with nanocrystalline silver membrane. After 7th day of surgery, clinical parameters will be checked and  circum-gingival plaque sample will be collected for Porphyromonas gingivalis count.

Surgical procedure

Subjects that meet the inclusion criteria will be chosen for flap surgery. Preoperatively patients will be given chlorhexidine mouthwash 0.12% (Perioguard) for mouth rinsing, before giving local anesthesia. All aseptic precautions and standard protocol will be followed during flap surgery. All the patients will be treated using 2% lignocaine hydrochloride with adrenaline in 1:80000 concentrations (Lignox 2% -A). Incision will be given with B.P. blade no. 15. on  the buccal and lingual surface and B.P. blade no 12 on the interdental region. Following reflection and debridement, the surgical site will be irrigated and flap approximation will begin using 3-0 silk sutures and covered with Acticoat ^TM under Barricaid®  or Coe-pak^TM   dressing materials. The photocured dressing will be dispensed at the juncture of the cervical one third of the teeth and the margin of the surgical site on the  facial and lingual aspect. It is then exposed to  visible light curing unit for 10 seconds per site per tooth until the entire dressing coverage was  checked prior to dismissing the patient. At the other surgical site, after drying with gauze  Coe-pak^TM   will be applied.

Post-operative instructions will be given. Antibiotics and analgesics would be administered. The dressing materials will be removed on the seventh day.

All clinical parameters will be monitored, and a circum-gingival plaque specimen will be obtained after the seventh day and stored in transport media for real-time PCR.

Postoperative recall

Subjects will be reviewed after 7^th day for evaluating clinical parameter and circum-gingival plaque sample will be collected after 7^th day of surgery.