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CTRI Number  CTRI/2022/10/046658 [Registered on: 20/10/2022] Trial Registered Prospectively
Last Modified On: 29/02/2024
Post Graduate Thesis  No 
Type of Trial  Interventional 
Type of Study   Drug 
Study Design  Randomized, Parallel Group, Placebo Controlled Trial 
Public Title of Study   A Study to see effect and safety of PNB-001 capsules in Inflammatory bowel disease 
Scientific Title of Study   A Phase II, Multicenter, Randomized, Double-blind, Placebo controlled Clinical study to evaluate efficacy and safety of PNB-001 as an adjunct to Mesalamine compared to Mesalamine alone in Subjects with Inflammatory bowel disease 
Trial Acronym   
Secondary IDs if Any  
Secondary ID  Identifier 
BCR-PNB-003 Version 1.0 dated 25 Mar 2022  Protocol Number 
 
Details of Principal Investigator or overall Trial Coordinator (multi-center study)  
Name  Dr Premashish Kar  
Designation  Principal Investigator 
Affiliation  Max Super Specialty Hospital, Vaishali 
Address  A Unit of Crosslay Remedies Ltd. W-3, Sector-1, Vaishali, Ghaziabad-201012, Utttar Pradesh, India

Ghaziabad
UTTAR PRADESH
201012
India 
Phone  9811042164  
Fax    
Email  premashishkar@gmail.com  
 
Details of Contact Person
Scientific Query
 
Name  Dr Neeta Nargundkar 
Designation  Managing Director 
Affiliation  Biosphere Clinical Research Pvt.Ltd 
Address  Office No. 02, 03 & 04, Second Floor,Highland Corporate Center,Kapurbawdi Junction, Thane (W) 400 607, Maharashtra, India

Thane
MAHARASHTRA
400607
India 
Phone  02241006794  
Fax    
Email  drneeta@biospherecro.com  
 
Details of Contact Person
Public Query
 
Name  Dr Neeta Nargundkar 
Designation  Managing Director 
Affiliation  Biosphere Clinical Research Pvt.Ltd 
Address  Office No. 02, 03 & 04, Second Floor,Highland Corporate Center,Kapurbawdi Junction, Thane (W) 400 607, Maharashtra, India


MAHARASHTRA
400607
India 
Phone  02241006794  
Fax    
Email  drneeta@biospherecro.com  
 
Source of Monetary or Material Support  
PNB Vesper Life Science Pvt. Ltd,Door No. 40/ 1045G, 5th floor,Amritha Towers, MG Road, KPCC Junction ,Kochi, Kerala 682011, India 
 
Primary Sponsor  
Name  PNB Vesper Life Science Pvt Ltd 
Address  Door No. 40/ 1045G, 5th floor,Amritha Towers, MG Road, KPCC Junction ,Kochi, Kerala 682011, India 
Type of Sponsor  Pharmaceutical industry-Indian 
 
Details of Secondary Sponsor  
Name  Address 
Biosphere Clinical Research Pvt Ltd  Office No. 02, 03 & 04, Second Floor, Highland Corporate Center, Kapurbawdi Junction, Thane (W) 400 607, Maharashtra, India 
 
Countries of Recruitment     India  
Sites of Study
Modification(s)  
No of Sites = 8  
Name of Principal Investigator  Name of Site  Site Address  Phone/Fax/Email 
Dr Sanjay Salunkhe  CIMETs Inamdar Mutispeciality Hospital  Department of Gastroenterology Hospital Building S.No .15,Behind KPCT Mall,Fatima Nagar ,Pune-411040, Maharashtra, India
Pune
MAHARASHTRA 
9823133390

sanjaysalunkhe@gmail.com 
Dr Vaibhav S Ganjewar  GI One Hospital  Third Floor, Amrut Sai Solitaire, Besides Goldie Cinema, Railway Station Road, Aurangabad-431005, Maharashtra, India
Aurangabad
MAHARASHTRA 
7720035616

drvaibhavganjewar@gmail.com 
DrTelli Venkata Pradeep Kumar  Great Eastern Medical School and Hospital, Srikakulam  Department of Gastroenterology,D.No 3-351,Ragolu, Srikakulam Andhra Pradesh - 532484 India
Srikakulam
ANDHRA PRADESH 
7406355777

drtvpradeepresearch@gmail.com 
Dr Ashok Dalal  Maulana Azad Medical College,(GIPMER)  Room A-210, Department of Gastroenterology, Academic Block,G.B.Pant Hospital,New Delhi-110002, India
New Delhi
DELHI 
9718599219

drashokdalal83@gmail.com 
Dr Premashish Kar   Max Super Specialty Hospital, Vaishali  Department of Gastroenterology and Hepatology, A Unit of Crosslay Remedies Ltd. W-3, Sector-1, Vaishali, Ghaziabad-201012, Utttar Pradesh, India
Ghaziabad
UTTAR PRADESH 
9811042164

premashishkar@gmail.com 
Dr Dharmendra B L  Mysore Medical College & Research Institute and Associated Hospitals  Department of Surgical Gastroenterology,K.R. Hospital, Irwin Road, Mysuru-570001, Karnataka, India.
Mysore
KARNATAKA 
9844400382

drdharmu21@gmail.com 
Dr Kaivan Shah   Navneet Memorial Hospital   Sushrusha, Opposite Sardar Patel Seva Samaj Hall, In the Lane, Opp. Telephone Exchange, Navrangpura, Ahmedabad-380009, Gujarat, India.
Ahmadabad
GUJARAT 
9833622433

drkaivanshah12@gmail.com 
Dr Prashant Rahate  Rahate Surgical Hospital  517, Kolbaswami Square, CA Road, Juni Mangalwari, Near Telephone Exchange, Nagpur-440008, Maharashtra, India.
Nagpur
MAHARASHTRA 
9822464068

prashantrahate84@yahoo.com 
 
Details of Ethics Committee
Modification(s)  
No of Ethics Committees= 8  
Name of Committee  Approval Status 
Ethics Committee of Navneet Memorial Hospital  Approved 
Ethics Committee Rahate Surgical Hospital   Approved 
Ikon Ethics Committee For research on human subject  Approved 
Institutional Ethics Committee, Maulana Azad Medical College, G. B. Pant Institute of Post Graduate Medical Education & Research (GIPMER),   Approved 
Institutional Ethics committee GEMS Srikakulam  Approved 
Institutional Ethics Committee, CIMET’s Inamdar Multispecialty Hospital  Submittted/Under Review 
Institutional Ethics Committee, Max Super Specialty Hospital  Approved 
Institutional Ethics Committee, Mysore Medical College & Research Institute  Approved 
 
Regulatory Clearance Status from DCGI  
Status 
Approved/Obtained 
 
Health Condition / Problems Studied  
Health Type  Condition 
Patients  (1) ICD-10 Condition: K509||Crohns disease, unspecified, (2) ICD-10 Condition: K519||Ulcerative colitis, unspecified,  
 
Intervention / Comparator Agent  
Type  Name  Details 
Comparator Agent  Placebo Capsules  One capsule three times daily with water after meals with the stable dose of oral Mesalamine of at least 2.4 gm/day for 4 weeks  
Intervention  PNB001 100mg   One capsule three times daily with water after meals with the stable dose of oral Mesalamine of at least 2.4 gm/day for 4 weeks  
Intervention  PNB001 50 mg   One capsule three times daily with water after meals with the stable dose of oral Mesalamine of at least 2.4 gm/day for 4 weeks  
 
Inclusion Criteria  
Age From  18.00 Year(s)
Age To  65.00 Year(s)
Gender  Both 
Details  1.Male or female Subjects aged between 18 and 65 years (both Inclusive).
2.Subjects who are able to understand and willing to sign informed consent for participation in the study and willing to adhere to all protocol procedures.
3.Subjects on stable dose of oral Mesalamine of at least 2.4 gm/day for 2 weeks prior to screening.
Inclusion Criteria specific for Ulcerative Colitis Participants (Cohort 1)
1.Subjects have a documented history of idiopathic ulcerative colitis based on standard endoscopic (colonoscopic) and histological criteria involving the whole/part of left side of the colon [approximately 60 cm up from the anus (anal verge) to splenic flexure of colon], with mild to moderate active disease].
2.Subjects with Ulcerative Colitis Disease Activity Index (UCDAI) score of 4-10.
3.Rectal bleeding score of 1 or more at baseline
4.Mucosal appearance score (based on endoscopy) of 1 point or more at baseline
5.Stool Frequency subscore of 1 or more at baseline.
Inclusion Criteria specific for Crohn’s Disease Participants(Cohort 2)
1.Subjects have a documented history of Crohns Disease(CD) established at least 3 months prior to randomization by clinical and endoscopic evidence and corraborated by a histopathology report
2.Has mild to moderately active CD as determined by Crohns Disease Activity Index (CDAI) score of CDAI ≥200 and ≤400
3.Average of greater than two liquid or soft stools per day and an abdominal pain intensity score >1 
 
ExclusionCriteria 
Details  1. Documented history of proximal or universal ulcerative colitis (pan colitis).
2. Subjects who demonstrate signs and symptoms of fulminant colitis, bowel stricture, toxic megacolon, an anticipated need for blood transfusion for gastrointestinal bleeding, or demonstrate evidence of peritonitis.
3. Bowel resection within the previous 6 months.
4. Subjects with hemoglobin of ≤ 10 g/dl at screening.
5. Subjects with ileostomy, colostomy, or known fixed symptomatic stenosis of the intestine.
6. Prior documented history of evidence of high grade dysplasia on biopsy from endoscopic examinations.
7. Subjects with the following clinically significant laboratory abnormalities: SGOT, SGPT,
Serum Bilirubin > 2.5 times the Upper Limit Normal (ULN)) at screening visit.
8. Subjectswith abnormal Sr.Creatinine value of ≥ 2 mg/dl at screening visit.
9. Subjects with current or recurrent Clostridium difficile infection.
10. History of biological therapy with agents like infliximab, adalimumab, etanercept, etc. within the previous 8 weeks of screening and Subjects in need of such therapy during the study period.
11. Subjects who received systemic steroids or immunosuppressant’s within the previous 4 weeks
of screening.
12. Subjects not able to withdraw and in need of continuing other immunosuppressant’s like sirolimus or cyclosporine during the study period.
13. Subjects with alcohol or drug abuse
14. Subjects with history of hepatitis B surface antigen (HBsAg) or hepatitis C antibody (anti-
HCV) positive, or other clinically active liver disease at screening.
15. Subjects with Type 1 Diabetes or Type 2 Diabetes Mellitus whose diabetes has not been stable
and uncontrolled for the previous three months and with HbA1c value greater than 8% at
screening.
16. Subjects with cerebrovascular disease in the previous 3 months
17. Subjects diagnosed or suffering from any Oncological Conditions since last 2 years will be
excluded from the study.
18. Subjects with pre-existent renal function disorders, liver function disorders, cardiac disease,
uncontrolled hypertension, clinically important hematological, metabolic, psychiatric, central
nervous system (CNS) or pulmonary disease.
19. Female subjects who are pregnant or lactating or planning to become pregnant during the study
period. Females who are not ready to use acceptable contraceptive methods during the course
of study
20. Subjects who participated in any other clinical or post-marketing study (not only for study
drugs but also for medical devices) 30 days before signing the informed consent
21. Any condition which the study physician judges to preclude safe participation in the study or to
confound the evaluation of the study outcome. 
 
Method of Generating Random Sequence   Computer generated randomization 
Method of Concealment   An Open list of random numbers 
Blinding/Masking   Participant and Investigator Blinded 
Primary Outcome  
Outcome  TimePoints 
Cohort 1: Ulcerative Colitis Disease
1.Proportion of Subjects achieving clinical response at week 4 in test arm as compared to placebo arm.

Cohort 2: Crohn’s Disease
1.Percentage of Participants with a Clinical Response (CDAI
Decrease from Baseline of ≥100 Points) at Week 4. 
Baseline, Day 14 and Day 28 
 
Secondary Outcome  
Outcome  TimePoints 
Cohort 1: Ulcerative Colitis Disease
1. Change from baseline in the Ulcerative Colitis Disease Activity Index (UCDAI) score at 4
weeks.
2. Change from baseline in Rectal bleeding score at 4 weeks.
3.Time to resolution of rectal bleeding within 4 weeks.
4.Change from baseline in Stool Frequency score at 4 weeks.
5.Time to normalization of stool frequency within 4 weeks.
6.Change from baseline in mucosal appearance score (based on colonoscopy) at 4 weeks.
7.Change in ESR, CRP, CD4, CD8, TNFα, IL6 and FC levels at 4 weeks from baseline.
8.Short Inflammatory Bowel Disease Questionnaire (SIBDQ) assessment 
28 Days 
Cohort 2: Crohn’s Disease
1.Change from baseline in the Crohns Disease Activity Index (CDAI) score at 4 weeks.
2.Change from baseline in Stool Frequency score at 4 weeks.
3.Change in ESR, CRP, CD4, CD8, TNF α, IL6 and FC levels at 4 weeks from baseline.
4.Change from baseline in Simple Endoscopic Score for Crohn’s Disease (SES-CD) (based on colonoscopy) at 4 weeks.
5.Short Inflammatory Bowel Disease Questionnaire (SIBDQ). 
28 Days 
The assessment of safety will be based on the frequency of Adverse Events and changes in
laboratory values. 
28 Days 
 
Target Sample Size   Total Sample Size="45"
Sample Size from India="45" 
Final Enrollment numbers achieved (Total)= "Applicable only for Completed/Terminated trials"
Final Enrollment numbers achieved (India)="Applicable only for Completed/Terminated trials" 
Phase of Trial   Phase 2 
Date of First Enrollment (India)   28/10/2022 
Date of Study Completion (India) Applicable only for Completed/Terminated trials 
Date of First Enrollment (Global)  Date Missing 
Date of Study Completion (Global) Applicable only for Completed/Terminated trials 
Estimated Duration of Trial   Years="0"
Months="6"
Days="0" 
Recruitment Status of Trial (Global)
Modification(s)  
Not Yet Recruiting 
Recruitment Status of Trial (India)  Open to Recruitment 
Publication Details   NIL 
Individual Participant Data (IPD) Sharing Statement

Will individual participant data (IPD) be shared publicly (including data dictionaries)?  

Response - NO
Brief Summary  
This study is a Phase II, Multicenter, Randomized, Double-blind, Placebo controlled Clinical study to evaluate the efficacy and safety of PNB-001 as an adjunct to Mesalamine compared to Mesalamine  alone in Subjects with Inflammatory bowel disease.
Primary objective:
- To evaluate the efficacy of PNB-001 as an adjunct to Mesalamine compared to Mesalamine alone in Subjects with Inflammatory bowel disease.
Secondary objectives:
- To evaluate the safety of PNB-001 as an adjunct to Mesalamine compared to Mesalamine alone in Subjects with Inflammatory bowel disease

Cohort 1: Ulcerative Colitis Disease
Primary Endpoints
- Proportion of Subjects achieving clinical response at week 4 in test arm as compared to placebo arm.
Secondary Endpoints
- Change from baseline in the Ulcerative Colitis Disease Activity Index (UCDAI) score at 4 weeks.
- Change from baseline in Rectal bleeding score at 4 weeks.
- Time to resolution of rectal bleeding within 4 weeks.
- Change from baseline in Stool Frequency score at 4 weeks.
- Time to normalization of stool frequency within 4 weeks.
- Change from baseline in mucosal appearance score (based on colonoscopy) at 4 weeks.
- Change in ESR, CRP, CD4, CD8, TNFα, IL6 and FC levels at 4 weeks from baseline.
- Short Inflammatory Bowel Disease Questionnaire (SIBDQ) assessment.
Cohort 2: Crohn’s Disease
Primary Endpoints
- Percentage of Participants with a Clinical Response (CDAI Decrease from Baseline of ≥100 Points) at Week 4.
Secondary Endpoints
- Change from baseline in the Crohn’s Disease Activity Index CDAI) score at 4 weeks.
- Change from baseline in Stool Frequency score at 4 weeks.
- Change in ESR, CRP, CD4, CD8, TNFα, IL6 and FC levels at 4 weeks from baseline.
- Change from baseline in Simple Endoscopic Score for Crohn’s Disease (SES-CD) (based on colonoscopy) at 4 weeks.
- Short Inflammatory Bowel Disease Questionnaire (SIBDQ)
 
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