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CTRI Number  CTRI/2022/08/044624 [Registered on: 04/08/2022] Trial Registered Prospectively
Last Modified On: 07/09/2023
Post Graduate Thesis  No 
Type of Trial  Interventional 
Type of Study   Nutraceutical 
Study Design  Randomized, Parallel Group, Placebo Controlled Trial 
Public Title of Study   Study to evaluate the efficacy and safety of Collavant n2 in individuals with osteoarthritis of the knee 
Scientific Title of Study   A randomised, double-blind, placebo-controlled study to evaluate the efficacy and safety of Collavant n2 in individuals with osteoarthritis of the knee 
Trial Acronym   
Secondary IDs if Any  
Secondary ID  Identifier 
BI/210903/COLL/OA Version: 1.0 Date: April 26, 2022  Protocol Number 
 
Details of Principal Investigator or overall Trial Coordinator (multi-center study)  
Name  Dr Shalini Srivastava 
Designation  Associate Director - Clinical Development 
Affiliation  Vedic Lifesciences Pvt Ltd 
Address  Vedic Lifesciences, 118, Morya House, Opp. Infinity mall, Andheri (West)

Mumbai (Suburban)
MAHARASHTRA
400053
India 
Phone  02242172300  
Fax    
Email  shalini.s@vediclifesciences.com  
 
Details of Contact Person
Scientific Query
 
Name  Dr Shalini Srivastava 
Designation  Associate Director - Clinical Development 
Affiliation  Vedic Lifesciences Pvt Ltd 
Address  Vedic Lifesciences, 118, Morya House, Opp. Infinity mall, Andheri (West)


MAHARASHTRA
400053
India 
Phone  02242172300  
Fax    
Email  shalini.s@vediclifesciences.com  
 
Details of Contact Person
Public Query
 
Name  Dr Sonal Raote 
Designation  Project lead 
Affiliation  Vedic Lifesciences Pvt Ltd 
Address  Vedic Lifesciences, 118, Morya House, Opp. Infinity mall, Andheri (West),

Mumbai (Suburban)
MAHARASHTRA
400053
India 
Phone  02242172300  
Fax    
Email  sonal.raote@vediclifesciences.com  
 
Source of Monetary or Material Support  
Vedic Lifesciences Pvt Ltd 
 
Primary Sponsor  
Name  Vedic Lifesciences Pvt Ltd 
Address  Vedic Lifesciences, 118, Morya House, Opp. Infinity mall, Andheri (West), 
Type of Sponsor  Contract research organization 
 
Details of Secondary Sponsor  
Name  Address 
NIL  NIL 
 
Countries of Recruitment     India  
Sites of Study
Modification(s)  
No of Sites = 11  
Name of Principal Investigator  Name of Site  Site Address  Phone/Fax/Email 
Dr Nikunj Dadhaniya  Amber Clinic  401-402, Fourth Floor, Santorini Square, Opp. Star Bazaar, Satellite Road, Ahmedabad – 380015,
Ahmadabad
GUJARAT 
9712999726

nikunjvdadhaniya@gmail.com 
Dr Sagar Karvir  Ayush Nursing Home  Gaurav Garden Complex, Row House 5, Gaurav Vista, Bandar, Pakhadi Road, Kandivali West, Mumbai - 400067,
Mumbai (Suburban)
MAHARASHTRA 
9821767824

sagar_karvir@yahoo.com 
Dr Sunil Vishwakarma  Care n Cure Multispeciality Hospital  1st floor, Kamdhenu Building, Opp. Bappa Sitaram Market, Achole Road, Nalasopara East, Palghar - 401209,
Thane
MAHARASHTRA 
8169122707

sunilvish55@gmail.com 
Dr Surendra Bhasale  Diamond Hospital  Mithchowki, Linking Road, Malad West, Mumbai-400064,
Mumbai (Suburban)
MAHARASHTRA 
9870418984

dr.surendrabhasale.ct@gmail.com 
Dr Bhushan Deshmukh  Ganga Superspeciality Hospital  Sai Square Mumbai Naka, Nashik, Maharashtra 422009
Nashik
MAHARASHTRA 
8421596968

kingbd89@gmail.com 
Dr Abdul Choudhary   Gayatri Hospital,  F001, Lavdeep Apartment, Waliv, Vasai East- 401208, Maharashtra, India
Mumbai
MAHARASHTRA 
8871853225

abdul83choudhary@gmail.com 
Dr Anteshwar Birajdar  Imperial Multispeciality Hospital  Pingle Pride, Near Radha Swami Ashram, Chikhali, Pune - 411062,
Pune
MAHARASHTRA 
9552595521

dranteshwarbirajdar@gmail.com 
Dr Ajinkya Desale  Life Care Hospital  New Nashik, Mumbai- Agra Highway Lekha Nagar Nashik - 422009
Nashik
MAHARASHTRA 
7666556070

desaleajinkya@gmail.com 
Dr Hrishikesh Patkar  Lifepoint Multispeciality Hospital  145/1 Mumbai Bangalore Highway Near Hotel Sayaji Wakad Pune - 411057
Pune
MAHARASHTRA 
9011267776

drhpatkar@yahoo.com 
Dr Kshitij Shah   Prime Speciality Hospital  Bonanza Residence junction, Off S V road Andheri west
Mumbai (Suburban)
MAHARASHTRA 
9833040048

dr.kshitij@hotmail.com 
Dr Rohit Nalavade  Sparsh Hospital  Plot no. 141, Sai Arcade, Mission Compound, Opposite Orion Mall, Panvel, Raigad- 410206
Raigarh
MAHARASHTRA 
7718984455

nrohit7@gmail.com 
 
Details of Ethics Committee
Modification(s)  
No of Ethics Committees= 11  
Name of Committee  Approval Status 
ACEAS Independent Ethics Committee  Approved 
ACEAS Independent Ethics Committee.  Approved 
ACEAS Independent Ethics Committee.  Approved 
Harmony Ethical research committee  Approved 
Harmony Ethical Research Committee.  Approved 
Harmony Ethical Research Committee.  Approved 
Harmony Ethical Research Committee.  Approved 
Lifepoint Research Ethics Committee.  Approved 
Lifepoint Research Ethics Committee.  Approved 
Muktai Hospital Ethics Committee  Approved 
Muktai Hospital Institutional Review Board.  Approved 
 
Regulatory Clearance Status from DCGI  
Status 
Not Applicable 
 
Health Condition / Problems Studied  
Health Type  Condition 
Patients  (1) ICD-10 Condition: M170||Bilateral primary osteoarthritis of knee,  
 
Intervention / Comparator Agent  
Type  Name  Details 
Intervention  Collavant n2 40 mg/day  2 capsules in the morning before breakfast and 2 capsules at night before going to bed for 180 days 
Comparator Agent  Glucosamine hydrochloride & Chondroitin sulfate  2 capsules in the morning before breakfast and 2 capsules at night before going to bed for 180 days 
Comparator Agent  Microcrystalline cellulose  2 capsules in the morning before breakfast and 2 capsules at night before going to bed for 180 days 
 
Inclusion Criteria  
Age From  40.00 Year(s)
Age To  75.00 Year(s)
Gender  Both 
Details  1. Male and females ≥ 40 to ≤ 75 years suffering from knee joint pain for atleast 3 months before screening.
2. Body mass index (BMI) ≥ 18.5 and ≤ 29.9 kg/m2
3. Pain VAS for knee joint pain ≥ 5 and ≤ 7 cm on a 10 cm scale at screening.
4. Radiographic evidence of grade II/III knee OA based on the Kellgren and Lawrence (KL) radiographic entry criteria for OA -
i Grade II: Antero-posterior weight-bearing knee radiograph demonstrates possible joint space narrowing (JSN) with definite osteophyte formation.
ii Grade III: Antero-posterior weight-bearing knee radiograph demonstrates definite joint space narrowing, multiple osteophyte formations, some sclerosis, and possible deformity of bony ends.
5. LAI score of ≥ 6 - ≤10 at screening.
6. Willing to stop the restricted dietary supplements, home-based remedies, and any other form of topical products or medications for knee joint pain relief or any other reason for the entire study duration.
(Note: only hot and cold fomentation will be allowed, and the participant has to record the daily frequency of fomentation in the diary. The hot/cold fomentation needs to be stopped 48 hours prior to all assessment visits.)
7. Willing to stop using rescue medication 48 hours prior to all assessment visits.
8. Using the western toilet at home and/or workplace.
9. Willingness to participate and comply with the study procedures and required visits.
10. Ability to understand and sign a written informed consent form, which must be completed before performing study-specific tasks.
11. Literate and have the ability to complete the study-based questionnaires and tasks.
12. Female participants of childbearing age must be willing to use the acceptable methods of contraception during the study. 
 
ExclusionCriteria 
Details  1 History of uncontrolled hypertension and/or systolic blood pressure ≥ 140
mmHg and/or diastolic blood pressure ≥ 90 mmHg.
2 Fasting blood glucose (FBG) > 125 mg/dl.
3 Radiographic evidence of Grade I or Grade IV OA based on the KL
radiographic criteria for osteoarthritis.
4 Any history of trauma, fractures, or surgery to the index joint.
5 Any planned surgery (diagnostic or therapeutic intervention) to the index
joint during the participation in the study.
6 History of use of corticosteroid, disease modifying drugs, glucosamine,
chondroitin, and intra-articular treatments including injections of
corticosteroid or hyaluronic acid within 6 months of the screening visit and
consumption of Omega-3 fatty acids or other joint health supplements within
15 days preceding the screening visit.
7 History of use of gabapentin within 6 weeks and/or methylcobalamin within
2 weeks prior to screening.
8 Known case of deformity of the knee joint or diagnosed on clinical
examination during screening.
9 Known case of any joint disorder involving the index joint that includes but
is not limited to known rheumatic or inflammatory conditions such as
rheumatoid arthritis, osteomyelitis, osteoporosis, severe OA, and bone
metastasis.
10 Known cases of gout and/or hyperuricemia (serum uric acid >440 μmol/L).
11 Other pathologic lesions on X-ray of the knee.
12 History of bleeding disorders (e.g., Haemophilia, Sickle cell anaemia, etc.).
13 Participants with abnormal levels of serum thyroid-stimulating hormone
(TSH) (< 0.4 to > 4.2 mIU/L).
14 Any history or evidence of allergy to chicken, eggs, or protein products in
the past.
15 Alcoholics or known drug dependents. (Alcoholism or heavy alcohol use is
interpreted based on alcohol content consumed per day or week. It is defined
as more than 4 drinks on any day or more than 14 drinks per week for men.
For women, it is defined as more than 3 drinks on any day or more than 7
drinks per week. Standard alcoholic drink is roughly equivalent to 14 grams
of pure alcohol, which is found in the following:
i 12 ounces (Approx. 350 ml) of regular beer, which is usually about 5%
alcohol
ii 5 ounces (Approx. 150 ml) of wine, which is typically about 12%
alcohol
iii 1.5 ounces (Approx. 45 ml) of distilled spirits, which is about 40%
alcohol)
16 History of smoking or currently smoking or using any form of smokeless
tobacco.
17 Not willing to abstain from the use of NSAIDs (including low dose aspirin
50 mg/day for cardiovascular health).
18 Participation in a study of an investigational product within 90 days prior to
the screening. 
 
Method of Generating Random Sequence   Computer generated randomization 
Method of Concealment   Sequentially numbered, sealed, opaque envelopes 
Blinding/Masking   Participant and Investigator Blinded 
Primary Outcome  
Outcome  TimePoints 
To evaluate the effect of 6-month (180 days) consumption of Collavant n2 on
knee joint health as assessed by change in Western Ontario and McMaster
University Osteoarthritis Index (WOMAC) total score compared to placebo. 
Day 0 and 180 
 
Secondary Outcome  
Outcome  TimePoints 
Knee joint pain as assessed by the change in the WOMAC - Pain subscale
(WOMAC-P) score. 
Day 0, 7, 30, 60, 90, 120, 150, and 180 
Knee joint stiffness as assessed by the change in the WOMAC - Stiffness
subscale (WOMAC-S) score. 
Day 0, 7, 30, 60, 90, 120, 150, and 180 
Knee joint function as assessed by the change in the WOMAC - Physical
function (WOMAC-PF) score. 
Day 0, 7, 30, 60, 90, 120, 150, and 180 
Knee joint pain assessed by the change in the Visual Analogue Scale (VAS)
score. 
Day 0, 7, 30, 60, 90, 120, 150, and 180 
Severity of osteoarthritis as assessed by the change in the Lequesne
algofunctional index (LAI) score. 
Day 0, 7, 30, 60, 90, 120,
150, and 180 
Overall health of the participant as assessed by change in the Participant’s
Global Assessment of Osteoarthritis (PGA-OA) 
Day 0, 30, 60, 90, 120,
150, and 180 
Percentage of responders as per OMERACT-OARSI responder index.  Day 180 
Cartilage metabolism determined by urine levels of C-terminal cross-linked
telopeptide of type II collagen (CTX-II). 
Day 0 and 180 
Use of rescue medication.  Day 7, 30, 60, 90, 120, 150, and 180 
Knee joint health as assessed by the change in total WOMAC score  Day 0, 7, 30, 60, 90, 120, 150, and 180 
 
Target Sample Size   Total Sample Size="255"
Sample Size from India="255" 
Final Enrollment numbers achieved (Total)= "0"
Final Enrollment numbers achieved (India)="236" 
Phase of Trial   N/A 
Date of First Enrollment (India)   07/08/2022 
Date of Study Completion (India) 04/07/2023 
Date of First Enrollment (Global)  Date Missing 
Date of Study Completion (Global) Date Missing 
Estimated Duration of Trial   Years="1"
Months="0"
Days="0" 
Recruitment Status of Trial (Global)
Modification(s)  
Not Applicable 
Recruitment Status of Trial (India)  Completed 
Publication Details   NA 
Individual Participant Data (IPD) Sharing Statement

Will individual participant data (IPD) be shared publicly (including data dictionaries)?  

Response - NO
Brief Summary  
This study is a randomized, double-blind study to evaluate efficacy and safety of the investigational product in individuals with grade II or grade III osteoarthritis of the knee. The study has three arms; investigational product arm, glucosamine hydrochloride + Chondroitin sulfate arm and placebo arm. 255 participants will be randomized into the 3 arms of the study. 
The Investigational product is Collavant n2, which consists of type II native collagen. The main aim of this study is to assess the effect of Collavant n2 on various aspects of joint health as compared to placebo and glucosamine arm
 
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