PRE SURGICAL PROCEDURE

—  Routine laboratory blood investigations will be carried out.

—  Phase l therapy, that will include oral hygiene instructions, scaling and root planing using hand and ultrasonic instruments, will be performed.

—  The CBCT measurements will be taken for each group i.e. Group A (Amniotic membrane and DFDBA) and Group B (Chorion membrane and DFDBA) at baseline and 24 weeks.

—  Patients will be re-evaluated 3-4 weeks after phase l therapy for plaque maintenance.

—  Patient with Plaque index³⁹ score <1.0 will be included in the study.

—  Appropriate antibiotic prophylaxis will be given to the patient before surgery.

SURGICAL TECHNIQUE

—  All surgical procedures will be carried out by one operator.

—  The sites will be randomly assigned to Group A or Group B through a computer generated data and will be assigned of either of the two groups before surgery.

—  CAF was advocated utilizing the method depicted by Pini-Prato et al.

—  The surgical area will be anaesthetised using 2% lignocaine hydrochloride containing 1:80,000 epinephrine. Before the elevation of the flap, both the exposed root surfaces will be gently planed with sharp Gracey curette to reduce root convexity. Immediately after, root conditioning will be done with 24% EDTA for 2-3 minutes⁴¹ followed by copious irrigation with normal saline for 30-60 seconds.

—  Intrasulcular incisions will be given with B P blade (no.15) on the buccal aspect of the involved tooth. This incision will be horizontally extended to the adjacent papillae avoiding the gingival margin of the adjacent teeth. Two vertical releasing incisions will be carried out from the mesial, and distal extremities of the horizontal incision sufficiently beyond the mucogingival junction.

·         A trapezoidal full�’ thickness flap will be raised with a periosteal elevator, 3-4mm beyond the apical crest of osseous dehiscence. Then a partial�’ thickness dissection will be carried out apically leaving the underlying periosteum in place.

—  In Group A amniotic membrane and DFDBA will be placed and the flap will be advanced coronally where as in Group B chorion membrane and  DFDBA will be placed followed by coronal flap advancement.

—  Suturing will be performed with 4-0 vicryl suture to secure the flap and membrane at the required position.

POST SURGICAL CARE:

—  Suitable antibiotic and analgesic will be prescribed for 5 to 7 days.

—  Patient will be advised to rinse with 0.2% chlorhexidine digluconate twice a day for 2 weeks following surgery.

—  Periodontal dressing and sutures will be removed 7-14 days after surgery.

—  Post operative instructions and patient will be reinstructed to maintain oral hygiene.

—  Each patient will be revaluated for periodontal maintenance and clinical parameters at 12 weeks and 24 weeks post operative will be recorded.

—  CBCT measurements will be recorded for each group i.e. Amniotic membrane and DFDBA group and Chorion membrane and DFDBA group after 6 months.