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CTRI Number  CTRI/2022/04/041823 [Registered on: 12/04/2022] Trial Registered Prospectively
Last Modified On: 27/10/2025
Post Graduate Thesis  No 
Type of Trial  Interventional 
Type of Study   Drug 
Study Design  Other 
Public Title of Study   Phase IIa study to evaluate the efficacy of the ISTH0036 in treatment naive and previously treated patients with Macular Degeneration and Diabetic Macular Edema 
Scientific Title of Study
Modification(s)  
TGF-BETa 2 antisense ISTH0036 for the Treatment of diabetic macular Edema (DME) and neovascular age-related maculaR degeneration (nAMD) as Monotherapy or in combination with Aflibercept The “BETTER” Study  
Trial Acronym   
Secondary IDs if Any
Modification(s)  
Secondary ID  Identifier 
ISTH-02-211, Version 2.1, 20 Apr 2022  Protocol Number 
 
Details of Principal Investigator or overall Trial Coordinator (multi-center study)  
Name  Dr Dharmesh Domadia 
Designation  Vice President – Global Clinical Sciences 
Affiliation  Cliantha Research Limited 
Address  Department Clinical Trials, Room no. 01, 2nd floor, 6, Arista@Eight Corporate House, Near Satyam House, Behind Rajpath Club, Bodakdev, Ahmadabad-380054, Gujarat, India.

Ahmadabad
GUJARAT
380054
India 
Phone  07966219500  
Fax  07966219500  
Email  ddomadia@cliantha.com  
 
Details of Contact Person
Scientific Query
 
Name  Dr Nil Desai 
Designation  Medical Monitor 
Affiliation  Cliantha Research Limited 
Address  Department Clinical Trials, Room no. 01, 2nd floor, 6, Arista@Eight Corporate House, Near Satyam House, Behind Rajpath Club, Bodakdev, Ahmadabad-380054, Gujarat, India.

Ahmadabad
GUJARAT
380054
India 
Phone  07966219500  
Fax  07966219500  
Email  nhdesai@cliantha.com  
 
Details of Contact Person
Public Query
 
Name  Devesh Verma 
Designation  Associate Director 
Affiliation  Cliantha Research Limited 
Address  Department Clinical Trials, 4th floor, 6, Arista@Eight Corporate House, Near Satyam House, Behind Rajpath Club, Bodakdev, Ahmadabad-380054, Gujarat, India.

Ahmadabad
GUJARAT
380054
India 
Phone  07966219500  
Fax  07966219500  
Email  dverma@cliantha.com  
 
Source of Monetary or Material Support  
Isarna Therapeutics GmbH Schellingstraße 109a 80798 Munich Germany  
 
Primary Sponsor  
Name  Isarna Therapeutics 
Address  GmbH Schellingstraße 109a 80798 Munich Germany 
Type of Sponsor  Pharmaceutical industry-Global 
 
Details of Secondary Sponsor  
Name  Address 
NILL  NILL 
 
Countries of Recruitment     India  
Sites of Study
Modification(s)  
No of Sites = 3  
Name of Principal Investigator  Name of Site  Site Address  Phone/Fax/Email 
Dr Manpreet Brar  Grewal eye Institute  Room Number : 151, Research Room, Basement Floor, Grewal eye Institute SCO 168-169 Sector 9C Chandigarh- 160009
Chandigarh
CHANDIGARH 
99988133646

Dr.manpreetbrarr@gmail.com 
Dr Muna Bhende   Medical Research Foundation, Sankara Netharalaya,Sri Bhagwan Mahavir Vitro Retina Services  Sri Bhagwan Mahavir Vitro Retina Services, Medical Research Foundation, Sankara Netharalaya 41/18, College Road, Nungambakkam
Chennai
TAMIL NADU 
9444177233

drmuna@snmail.org 
DrVishali Gupta  Postgraduate Institute of Medical Education and research   PGIMER, Sector 12, Room no. 233, Second floor, Advanced eye center, Chandigarh, 160012
Chandigarh
CHANDIGARH 
9417565506

vishalisara@gmail.com 
 
Details of Ethics Committee
Modification(s)  
No of Ethics Committees= 3  
Name of Committee  Approval Status 
IIH- Institutional Ethics Committee Indus International Hospital  Approved 
Institutional Ethics Committee Post Graduate Institute of Medical Education and Research  Approved 
Institutional Review Board ( EC) of Vision Research Foundation  Approved 
 
Regulatory Clearance Status from DCGI
Modification(s)  
Status 
Approved/Obtained 
 
Health Condition / Problems Studied  
Health Type  Condition 
Patients  (1) ICD-10 Condition: E133||Other specified diabetes mellituswith ophthalmic complications,  
 
Intervention / Comparator Agent  
Type  Name  Details 
Intervention  ISTH0036  The duration of study participation is 9 months preceded by an up to 40 days screening period. Screening and Baseline Visit may be combined. Last treatment is at month 6 and the Primary Endpoint will be assessed at month 7. After termination of ISTH0036 treatment, the End of Study Visit takes place 90 days post last dosing (i.e., month 9).  
Comparator Agent  NA  NA 
 
Inclusion Criteria
Modification(s)  
Age From  18.00 Year(s)
Age To  50.00 Year(s)
Gender  Both 
Details  Patients eligible for inclusion in this study must meet all the following criteria:
1. Patients must provide written informed consent before any study related procedures are performed.
2. Patients must be ≥18 yrs in the DME cohort and ≥ 50 in the nAMD cohort at Screening.

Study eye nAMD:
3. Active CNV lesions secondary to nAMD (including retinal angiomatous proliferation lesions with a CNV component) in the study eye at Screening in treatment naïve nAMD arm.
4. About 50% of the patients in the AMD treatment naïve group should have classic or predominantly classic CNV / Type II CNV or hemorrhage
5. Pretreated CNV lesions secondary to nAMD (including retinal angiomatous proliferation lesions with a CNV component) in the study eye at Screening in pretreated nAMD. About 50% of the patients should be included with SHRM present on OCT despite anti-VEGF therapy or hemorrhage present at initial diagnosis/ therapy start.

6. Intra- and/or subretinal fluid affecting the central subfield of the study eye at Screening with CMT ≥305 μm in women, and ≥320μm in men or with a center point thickness of >260 in women and >270 micrometer in men as measured by SD-OCT in treatment naïve nAMD arm.
7. For treatment naïve patients, BCVA between 83 and 23 letters, inclusive, in the study eye at Screening and Baseline using early treatment diabetic retinopathy study (ETDRS) testing.
8. For Treatment naïve and VEGF primed group: Treatment naïve nAMD in study eye
9. For VR-group: Dry or ≤50 micrometer SRF in vertical extension after receiving 1-6 anti-VEGF injections. Diagnosis no longer than 9 months before Baseline

Study eye DME:
10. Active CME secondary to diabetes in the study eye at Screening in treatment naïve DME.
11. Intra- and/or subretinal fluid affecting the central subfield of the study eye at Screening with CMT ≥305 μm in women, and ≥320μm in men or with a center point thickness of >260 in women and >270 micrometer in men as measured by SD-OCT in treatment naïve DME arm.
12. For treatment naïve patients, BCVA between 83 and 23 letters, inclusive, in the study eye at Screening and Baseline using early treatment diabetic retinopathy study (ETDRS) testing.
13. Moderate to severe NPDRP assessed via ETDRS severity scale and mild PDRP (with 1 NVE) in study eye (diabetic retinopathy severity scale DRSS 43-61)
14. Absence of prior PRP treatment
15. For Treatment naïve group: Treatment naïve DME in study eye
16. For VR-group: History of CME secondary to diabetes in the study eye with complete resolution of IRF and/or SRF or a ≥20% reduction in CMT secondary to diabetes after receiving 1-6 monthly anti-VEGF injections over the last 9 months.
 
 
ExclusionCriteria 
Details  Patients meeting any of the following criteria are not eligible for inclusion in this study.
Ocular conditions
1. Any active intraocular or periocular infection or active intraocular inflammation (e.g., infectious conjunctivitis, keratitis, scleritis, endophthalmitis, infectious blepharitis) in either eye at Baseline.
2. Presence of amblyopia, amaurosis or ocular disorders in the fellow eye with BCVA < 35 ETDRS letters at Screening (except when due to conditions whose surgery may improve visual acuity, e.g., cataract).

Study eye nAMD
3. nAMD diagnosed for more than 9 months
4. Poor quality of SD-OCT images at Screening or Baseline.

5. Central subfield of the study eye affected by geographic atrophy assessed by OCT, color fundus photography (CFP) and fundus autofluorescence (FAF) at Screening.
6. Subretinal blood affecting the central subfield and/or ≥ 50% of the lesion of the study eye at Screening.
7. Concomitant conditions or ocular disorders in the study eye, including retinal diseases other than nAMD, that, in the judgment of the investigator, could require medical or surgical intervention during the study to prevent or treat visual loss that might result from that condition, or that limits the potential to gain visual acuity upon treatment with the investigational product.
8. Structural damage within 0.5-disc diameter of the center of the macula in the study eye, e.g., vitreomacular traction, epiretinal membrane, retinal pigment epithelium (RPE) rip/tear scar, laser burn, at the time of Screening that in the investigator’s opinion could preclude visual function improvement with treatment.
9. Current vitreous hemorrhage or history of vitreous hemorrhage in the study eye within 4 weeks prior to Baseline.
10. Uncontrolled glaucoma in the study eye defined as IOP > 25 mmHg on medication or according to the investigator’s judgment at Screening or Baseline.
11. Aphakia and/or complete absence of the posterior capsule in the study eye at Screening.
12. For Treatment naïve and VEGF primed group: Any prior treatment for nAMD in study eye
13. For VR-group: > 50 micrometer SRF in vertical extension after receiving 1-6 anti-VEGF injections over the last 9 months
14. For VR-group: > 6 anti-VEGF injections
Study eye DME
15. Treatment requiring DME diagnosed for more than 9 months
16. Poor quality of SD-OCT images.
17. Focal laser scars within the central 1 mm ETDRS subfield
18. Concomitant conditions or ocular disorders in the study eye, including retinal diseases other than DME, that, in the judgment of the investigator, could require medical or surgical intervention during the study to prevent or treat visual loss that might result from that condition, or that limits the potential to gain visual acuity upon treatment with the investigational product.
19. Structural damage within 0.5-disc diameter of the center of the macula in the study eye, e.g., vitreomacular traction, epiretinal membrane, retinal pigment epithelium (RPE) rip/tear scar, laser burn, at the time of Screening that in the investigator’s opinion could preclude visual function improvement with treatment.
20. Current vitreous hemorrhage or history of vitreous hemorrhage in the study eye within 4 weeks prior to Baseline.
21. Uncontrolled glaucoma in the study eye defined as IOP > 25 mmHg on medication or according to the investigator’s judgment at Screening or Baseline.
22. Moderate and severe PDRP requiring PRP in study eye
23. Prior PRP treatment in study eye

24. Aphakia and/or complete absence of the posterior capsule in the study eye at Screening.
25. For Treatment naïve group: Any prior treatment for DME in study eye
26. For VR-group: <20% reduction in CMT after receiving 1-6 monthly anti-VEGF injections over the last 9 months after diagnosis.
27. For VR-group: > 6 anti-VEGF injections
Ocular treatments (study eye)
28. Patient has received any investigational treatment for nAMD or DME (other than vitamin supplements) in the study eye at any time.
29. Intraocular or periocular use of corticosteroids in the study eye during the 6-month period prior to Baseline.
30. Previous penetrating keratoplasty or vitrectomy at any time prior to Baseline.
31. History or evidence of the following in the study eye within the 90-day period prior to Baseline:
• Intraocular or refractive surgery.
• Previous submacular surgery, other surgical intervention, or laser treatment for nAMD or DME including photodynamic therapy (PDT) and focal laser treatment.
Systemic conditions or treatments
32. End stage renal disease requiring dialysis or renal transplant.
33. Systemic medications known to be toxic to the lens, retina, or optic nerve (e.g., deferoxamine, chloroquine/hydroxychloroquine, tamoxifen, phenothiazines and ethambutol) used during the 6-month period prior to Baseline.
34. Participation in an investigational drug, biologic, or device study within 30 days or the duration of 5 half-lives of the investigational product (whichever is longer) prior to Baseline. Note: observational clinical studies solely involving over-the-counter vitamins, supplements, or diets are not exclusionary.
35. Systemic anti-VEGF therapy at any time.
36. Stroke or myocardial infarction in the 6-month period prior to Baseline.
37. Uncontrolled blood pressure defined as a systolic value ≥ 170 mmHg or diastolic value ≥ 110 mmHg at Screening or Baseline. (In case there is an elevated blood pressure measurement, it should be repeated after 20 minutes. If the repeat measurement is elevated, then the patient is not eligible to be enrolled into the study).
38. History of a medical condition (disease, metabolic dysfunction with exception of type 1 or 2 diabetes mellitus, physical examination finding, or clinical laboratory finding) that, in the judgment of the investigator, would preclude scheduled study visits, completion of the study, or a safe administration of investigational product.
39. History of malignancy of any organ system (other than localized basal cell carcinoma of the skin or in situ cervical cancer), treated or untreated, within the past 5 years, regardless of whether there is evidence of local recurrence or metastases.
40. History of hypersensitivity to any component of the test article, control article, or clinically relevant sensitivity to fluorescein dye, as assessed by the investigator
Other
41. Pregnant or nursing (lactating) women, where pregnancy is defined as the state of a female after conception and until the termination of gestation, confirmed by a positive urine pregnancy test.
42. Women of childbearing potential defined as all women less than 1 year postmenopausal or less than 6 weeks since sterilization at Baseline, unless they are using effective methods of contraception during dosing of study treatment. Effective contraception methods include:
• Total abstinence (when this is in line with the preferred and usual lifestyle of the patient). Periodic abstinence (e.g., calendar, ovulation, symptothermal, postovulation methods) and withdrawal are not acceptable methods of contraception.
• Female sterilization (have had surgical bilateral oophorectomy with or without hysterectomy) or tubal ligation at least 6 weeks before Baseline. In case of oophorectomy alone, only when the reproductive status of the woman has been confirmed by follow-up hormone level assessment.
• Male sterilization (at least 6 months prior to Baseline). For female patients in the study, the vasectomized male partner should be the sole partner for that patient.
• Barrier methods of contraception: condom or occlusive cap (diaphragm or cervical/vault caps) with spermicidal foam/gel/film/cream/vaginal suppository.

• Use of oral, injected or implanted hormonal methods of contraception or other forms of hormonal contraception that have comparable efficacy (failure rate < 1%), for example, hormone vaginal ring or transdermal hormone contraception.
• Placement of an intrauterine device or intrauterine system.
In case of use of oral contraception women should have been stable on the same pill for a minimum of 3 months before taking study treatment.
Women are considered post-menopausal and not of childbearing potential if they have had 12 months of natural (spontaneous) amenorrhea with an appropriate clinical profile or have had surgical bilateral oophorectomy (with or without hysterectomy), total hysterectomy, or tubal ligation at least 6 weeks before taking study treatment. In the case of oophorectomy alone, only when the reproductive status of the woman has been confirmed by follow-up hormone level assessment is she considered not of childbearing potential.
 
 
Method of Generating Random Sequence   Not Applicable 
Method of Concealment   Not Applicable 
Blinding/Masking   Not Applicable 
Primary Outcome  
Outcome  TimePoints 
OCT CMT at 7 months vs. baseline. For treatment naïve patients, improvement versus BL will be analyzed, for anti-VEGF pre-treated patients, maintenance, or improvement of CMT versus BL will be assessed.   9 Months 
 
Secondary Outcome  
Outcome  TimePoints 
Number and type of adverse events (AE) in all groups
Incidence and progression of cataract using LOCS III score in patients treated with ISTH0036
Change in BCVA from baseline up to month 9
Change of central macular volume up to month 9
 
9 Months  
 
Target Sample Size
Modification(s)  
Total Sample Size="43"
Sample Size from India="22" 
Final Enrollment numbers achieved (Total)= "43"
Final Enrollment numbers achieved (India)="22" 
Phase of Trial   Phase 2 
Date of First Enrollment (India)   17/04/2022 
Date of Study Completion (India) 22/06/2024 
Date of First Enrollment (Global)  Date Missing 
Date of Study Completion (Global) 22/06/2024 
Estimated Duration of Trial   Years="1"
Months="9"
Days="0" 
Recruitment Status of Trial (Global)
Modification(s)  
Completed 
Recruitment Status of Trial (India)  Completed 
Publication Details   Nill 
Individual Participant Data (IPD) Sharing Statement

Will individual participant data (IPD) be shared publicly (including data dictionaries)?  

Response - NO
Brief Summary
Modification(s)  
Considering the efficacy and safety findings demonstrated in the phase 1 study and the preclinical program, this Phase 2a multicenter study of treatment-naïve and pretreated patients with DME and nAMD is being performed to assess effects of ISTH0036 as monotherapy or in combination with Aflibercept on macular edema, CNV and fibrosis over a period of 7 months (plus additional 2 mo of safety observation).[
 
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