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CTRI Number  CTRI/2022/02/040015 [Registered on: 04/02/2022] Trial Registered Prospectively
Last Modified On: 08/04/2026
Post Graduate Thesis  No 
Type of Trial  Interventional 
Type of Study   Drug 
Study Design  Randomized, Parallel Group, Placebo Controlled Trial 
Public Title of Study   Study on patient with active proliferative Lupus nephritis on treatment with SOC with Mycophenolate Mofetil (MMF) and glucocorticoids with anifrolumab to compare the safety and efficacy. 
Scientific Title of Study   A Multicenter Randomized Double-Blind Placebo-Controlled Phase 3 Study to Evaluate the Efficacy and Safety of Anifrolumab in Adult Patients with Active Proliferative Lupus Nephritis 
Trial Acronym   
Secondary IDs if Any  
Secondary ID  Identifier 
D3466C00001 V 1.0 Date 28 July 2021  Protocol Number 
NCT05138133  ClinicalTrials.gov 
 
Details of Principal Investigator or overall Trial Coordinator (multi-center study)  
Name  Dr Santosh Kadam 
Designation  Country Director, Clinical Operations 
Affiliation  AstraZeneca Pharma India Ltd 
Address  Block N1 12th Floor Manyata Embassy Business Park Rachenahalli Outer Ring Road Bangalore

Bangalore
KARNATAKA
560045
India 
Phone  9535999494  
Fax    
Email  santosh.kadam@astrazeneca.com  
 
Details of Contact Person
Scientific Query
 
Name  Dr Santosh Kadam 
Designation  Country Director, Clinical Operations 
Affiliation  AstraZeneca Pharma India Ltd 
Address  Block N1 12th Floor Manyata Embassy Business Park Rachenahalli Outer Ring Road Bangalore

Bangalore
KARNATAKA
560045
India 
Phone  9535999494  
Fax    
Email  santosh.kadam@astrazeneca.com  
 
Details of Contact Person
Public Query
 
Name  Dr Santosh Kadam 
Designation  Country Director, Clinical Operations 
Affiliation  AstraZeneca Pharma India Ltd 
Address  Block N1 12th Floor Manyata Embassy Business Park Rachenahalli Outer Ring Road Bangalore

Bangalore
KARNATAKA
560045
India 
Phone  9535999494  
Fax    
Email  santosh.kadam@astrazeneca.com  
 
Source of Monetary or Material Support  
AstraZeneca AB 151 85 Sodertalje, Sweden  
AstraZeneca KK 3-1, Ofuka-cho, Kita-ku, Osaka, Japan 530-0011  
 
Primary Sponsor  
Name  AstraZeneca AB and AstraZeneca KK 
Address  AstraZeneca AB 151 85 Sodertalje, Sweden AstraZeneca KK 3-1, Ofuka-cho, Kita-ku, Osaka, Japan 530-0011 
Type of Sponsor  Pharmaceutical industry-Global 
 
Details of Secondary Sponsor  
Name  Address 
AstraZeneca Pharma India Ltd  Block N1, 12th Floor, Manyata Embassy Business Park Rachenahalli, Outer Ring Road, Bangalore – 560045, India  
 
Countries of Recruitment     Viet Nam
Argentina
Belgium
Bulgaria
China
France
Germany
Hungary
India
Italy
Japan
Mexico
Netherlands
Peru
Poland
Russian Federation
Taiwan
Thailand
Turkey
United States of America  
Sites of Study
Modification(s)  
No of Sites = 15  
Name of Principal Investigator  Name of Site  Site Address  Phone/Fax/Email 
Dr Umesh Gupta  Aakash Healthcare Private Limited  Dept. of Nephrology, Hospital Plot, Road No. 201 Dwarka Sector - 3 New Delhi, PIN– 110075
New Delhi
DELHI 
9650944544

drumesh.gupta@aakashhealthcare.com 
Dr Uma Kumar  All India Institute of Medical Sciences, New Delhi  Room no. 4076, 4th Floor, Teaching Block, Ansari Nagar PIN- 110029
New Delhi
DELHI 
9869217040

umaakumar@yahoo.co.in 
Dr Geetabali Sircar  Institute of Post Graduate Medical Education & Research (IPGME&R) and SSKM hospital  Dept. of Rheumatology, 244, AJC Bose Road, PIN 700020
Kolkata
WEST BENGAL 
03322041100

geet4in@yahoo.co.in 
Dr Nachiket Kulkarni  Jehangir Clinical Development Centre Pvt Ltd  Jehangir Hospital Premises, 32 Sassoon Road, Pune-411001
Pune
MAHARASHTRA 
9923243860

drnachiketkulkarni@gmail.com 
Dr Sarath Chandra Mouli Veeravalli  Krishna Institute of Medical Sciences Limited, Secunderabad  1-8-31/1, Minister Road, Secunderabad- PIN 500003
Hyderabad
TELANGANA 
9866000685

sarath10@hotmail.com 
Dr Kamal Goplani  Kusum Dhirajlal Hospital (KD Hospital)  Department of Nephrology, Kusum Dhirajlal Hospital (KD Hospital), Vaishnavdevi Circle, S.G. Highway, Khodiar, Ta. Dascroi, Dist. Ahmedabad, PIN– 382421 India
Ahmadabad
GUJARAT 
9909975020

kamalgoplani@gmail.com 
Dr Dinesh Khullar  Max Super Speciality Hospital  Department of Nephrology, Saket, New Delhi – 110017.
New Delhi
DELHI 
9810124066

drdineshkhullar@gmail.com 
Dr Vijay Kumar Kher  Medanta – The Medicity  Department of Nephrology, Sector 38, Gurgaon, Haryana – 122001, India.
Gurgaon
HARYANA 
9810054118

vijay.kher51@gmail.com 
Dr Sishir Gang   Muljibhai Patel Urological Hospital  Department of Nephrology, Dr. Virendra Desai road, Nadiad-387001 Gujarat, India.
Rajkot
GUJARAT 
9998439518

sishirgang@hotmail.com 
Dr Liza Rajasekhar  Nizam’s Institute of Medical Sciences  Professor & HOD, Department of Clinical Immunology and Rheumatology, Panjagutta, PIN- 500082
Hyderabad
TELANGANA 
04023489000

lizarajasekhar@gmail.com 
Dr A Ramakrishnam Naidu  Queens NRI Hospital  Clinical Research Room, Gurudwara Lane, Seethammadara, PIN-530013,
Visakhapatnam
ANDHRA PRADESH 
9885093386

rammky@yahoo.com 
Dr Priyanka Prajapati  Sheth Vadilal Sarabhai General Hospital  Madalpur Gam, Paldi Road, Ellisbridge, Ahmedabad, PIN – 380006
Ahmadabad
GUJARAT 
8866042949

priyankaprajapati2761995@gmail.com 
Dr Vivek Ruhela   Shri Mahant Indiresh Hospital  Department of Nephrology, Patel Nagar, Dehradun – 248001 Uttarakhand India
Dehradun
UTTARANCHAL 
9721104868

vivekruhela@yahoo.com 
Dr Atul Kakar  Sir Ganga Ram Hospital  Old Rajinder Nagar, New Rajinder Nagar, PIN- 110060
New Delhi
DELHI 
9811110802

atulkakar@hotmail.com 
Dr Krishna MVS  Sunrise Hospital,   Department of Nephrology, Bellapu Sobhanadri Road, Near Pushpa Hotel Centre, Swathi Weekly Press Centre, Vijayawada- 520002, Andhra Pradesh, India
Visakhapatnam
ANDHRA PRADESH 
9848148967

drsaikrishnamaddi@gmail.com 
 
Details of Ethics Committee
Modification(s)  
No of Ethics Committees= 15  
Name of Committee  Approval Status 
Aakash Healthcare Institutional Ethics Committee  Approved 
Ethics Committee Jehangir Clinical Development Centre Pvt Ltd., Jehangir Hospital Premises, 32 Sassoon Road, Pune - 1  Approved 
Ethics Committee Sir Ganga Ram Hospital   Approved 
Institutional Ethics Committee Muljibhai Patel Society For Research In Nephro-Urology Jayaramdas Patel Academic Centre, Muljibhai Patel Urological Hospital Dr Virendra Desai Road, Nadiad -387001 Kheda Gujarat  Approved 
Institutional Ethics Committee Queens NRI Hospital  Approved 
institutional ethics committee sunrise hopitals(IEC-SH) Opp.corporation bank bellapusobhanadri Road, near pushpa hotel centre, suryaraop eta,Vijayawada-520002 ,Andhra Pradesh, India  Approved 
Institutional Ethics Committee, AIIMS  Approved 
Institutional Ethics Committee, Shri Guru Ram Rai Institute of Medical and Health Sciences, Administrative Building, Patel Nagar, Dehradun 248001, Uttrakhand, India  Approved 
IPGME&R Oversight Committee  Approved 
KD Hospital Institutional Ethics Committee Kusum Dhirajlal Hospital (KD Hospital), Vaishnavdevi Circle, S.G. Highway, Khodiar, Ta. Dascroi, Dist. Ahmedabad, Gujarat – 382421  Approved 
KIMS Ethics Committee, Secunderabad  Approved 
Max Healthcare Ethics Committee, Ground Floor, Office of Ethics Committee, West Block, Near MS Office, Max Super Speciality Hospital, (Unit of Max Healthcare Institute Limited) 1- Press Enclave Road, Saket New Delhi, Delhi 110017  Approved 
Medanta Independent Ethics Committee Sector 38, Gurgaon, Haryana – 122001, India  Submittted/Under Review 
NIMS Institutional Ethics Committee  Approved 
Sangini Hospital Ethics Committee Sangini Hospital  Approved 
 
Regulatory Clearance Status from DCGI  
Status 
Approved/Obtained 
 
Health Condition / Problems Studied  
Health Type  Condition 
Patients  (1) ICD-10 Condition: D899||Disorder involving the immune mechanism, unspecified,  
 
Intervention / Comparator Agent  
Type  Name  Details 
Intervention  Anifrolumab  Anifrolumab + Standard of Care (Mycophenolate Mofetil (MMF)+ Glucocorticoid) Frequency-Every 4 weeks, Route of administration: IV and Duration: Week 0 to 100. 
Comparator Agent  Placebo  Placebo + Standard of Care (Mycophenolate Mofetil (MMF)+ Glucocorticoid) Frequency-Every 4 weeks, Route of administration: IV and Duration: Week 0 to 100. 
 
Inclusion Criteria  
Age From  18.00 Year(s)
Age To  70.00 Year(s)
Gender  Both 
Details  Informed consent- 1. Capable of giving signed informed consent prior to performing any protocol-related procedures, including Screening evaluations
2. Completion of Screening procedures within 30 days after signing the ICF.
Age and Sex- Males and females aged 18 to 70 years of age.
Disease Characteristics- 1. Fulfills updated 2019 SLE criteria
2. Have positive ANA, anti-dsDNA, or anti-Sm test result in sample obtained during Screening
3. Urine protein to creatinine ratio > 1 mg/mg (113.17 mg/mmol)
4. Have active proliferative LN Class III or IV either with or without the presence of Class V
5.Adequate peripheral venous access
6. eGFR ≥ 35 mL/min/1.73 m2 (as calculated by the Chronic Kidney Disease Epidemiology Collaboration formula)
Tuberculosis 1. Chest radiograph or a CT scan of the chest
that meets all of the following criteria:No evidence of current active infection (eg, pneumonia, TB) or previous TB; and No evidence of malignancy; and No CS abnormalities (unless due to SLE)
2. Meets all of the following TB criteria:
No signs or symptoms of active TB prior to or during any Screening visit
No medical history or past physical examinations suggestive of active TB
A chest radiograph during the Screening Period or within 12 weeks prior to signing the ICF with no evidence of active or signs of prior TB infection
No recent contact with a person with active TB OR if there has been such contact, referral to a physician specializing in TB to undergo additional evaluation prior to Week 0 (Day 1) and, if warranted, receipt of appropriate treatment for latent TB at or before the first administration of study intervention
No history of latent TB prior to signing the ICF, with the exception of latent TB with documented completion of appropriate treatment.
COVID-19 - Negative SARS-Cov-2 RT-PCR test result at Screening and no known or suspected COVID-19 infection or exposure between signing the ICF and Week 0 (Day 1).
Weight Body weight ≥ 40.0 kg
Pap Smear- Females with an intact cervix must have documentation of a Pap smear with no documented malignancy (eg, CIN III, carcinoma in situ, or adenocarcinoma in situ) within 2 years prior to Week 0 (Day 1)
Contraceptive Requirements Contraceptive use by men or women should be consistent with local regulations regarding the methods of contraception for those participating in clinical studies
Male participants:
All males (sterilized or non-sterilized) who are sexually active must use condom (with spermicide, where commercially available for contraception) from Day 1 until at least 12 weeks after receipt of the final dose of study intervention and until at least 90 days after the last dose of Sponsor-provided MMF
Male participants must not donate sperm during the course of the study and for 12 weeks after the last dose of the study intervention and 90 days after the last dose of Sponsor-provided MMF
Female participants:
females of childbearing potential must have Negative serum β-hCG test at Screening.
Women of childbearing potential must have a negative urine pregnancy test at Week 0 (Day 1), prior to administration of study intervention and prior to the first dose of Sponsor-provided MMF.
Females of childbearing potential must use 2 effective methods of avoiding pregnancy, only one of which is a barrier method, from Screening until 12 weeks after the final dose of study intervention and from the first dose of Sponsor-provided MMF until 6 weeks after the last dose of Sponsor-provided MMF unless the participant is surgically sterile (eg, bilateral oophorectomy or complete hysterectomy), has a sterile male partner, is at least one year
postmenopausal, or practices sustained abstinence consistent with the participant’s customary lifestyle.  
 
ExclusionCriteria 
Details  Medical Conditions- 1. A diagnosis of pure Class V LN based on the renal biopsy obtained within 6 months prior to signing the ICF or during Screening
2. History of dialysis within 12 months prior to signing the ICF or expected need for renal replacement therapy
3 History of, or current renal diseases (other than LN) that, in the opinion of the Investigator, could interfere with the LN assessment and confound the disease activity assessment (eg, diabetic nephropathy)
infection and malignancy risk factors-
1.History of recurrent infection requiring hospitalization and/or IV antibiotics
2. Known history of a primary immunodeficiency, splenectomy, or any underlying condition that predisposes the participant to infection, or a positive result for HIV infection confirmed by the central laboratory at Screening
3. At Screening, confirmed positive test for hepatitis B serology, as confirmed by central laboratory,
4. Positive test for hepatitis C antibody as confirmed by the central laboratory
5. Any clinical CMV or EBV infection that has not completely resolved within 12 weeks prior to signing the ICF
6. Opportunistic infection requiring hospitalization or IV antimicrobial treatment within 3 years prior to signing the ICF
7. Any severe case, of HZ infection at any time prior to Week 0
Systemic HZ such as herpes encephalitis, ophthalmic herpes involving the retina at any time prior to Week 0 (Day 1)
8. Clinically significant chronic infection (ie, osteomyelitis, bronchiectasis, etc
9. Any infection requiring oral anti-infectives
10. History of cancer, apart from:
Squamous or basal cell carcinoma of the skin treated with documented success of curative therapy ≥ 3 months prior to Week 0 (Day 1)
Cervical cancer in situ treated with apparent success with curative therapy ≥ 1 year prior to Week 0 (Day 1)
COVID-19: 1. Any history of severe COVID-19 infection, eg, requiring hospitalization, intensive care unit care, or assisted ventilation, or any prior COVID-19 infection with unresolved sequelae
2. Confirmed or probable COVID-19 infection
3. Failure to comply with all required Screening procedures due to circumstances related to pandemic or public health emergency.
Prior/Concomitant Therapy- 1. Prior receipt of anifrolumab
2. Previous receipt of >â—¦2 investigation treatments (other than anifrolumab) for LN or SLE since time of diagnosis and through signing the ICF
3. Known intolerance to ≤ 1.0 g/day of MMF
4. Receipt of any commercially available biologic agent within 5 half-lives prior to signing of the ICF
5. Receipt of any of the following prior to signing the ICF:
Receipt of B cell-depleting therapy (eg, rituximab or obinutuzumab):
6. A known history of allergy or reaction to any component of the study intervention formulation or history of anaphylaxis to any human gamma globulin therapy, human proteins, or monoclonal antibodies
7. Receipt of any of the following:
Any live or attenuated vaccine within 8 weeks prior to signing the ICF
Any restricted medication
Blood transfusion within 4 weeks prior to signing the ICF
Any of the following for the current LN flare (ie, since the qualifying renal biopsy):
o IV cyclophosphamide > 2 pulses of high-dose (≥ 0.5 g/m2) or > 4 doses of low-dose (500 mg every 2 weeks) or
o Average MMF > 2.5 g/day (or > 1800 mg/day of enteric coated mycophenolate sodium) for more than 8 weeks or
o Tacrolimus > 4 mg/day for more than 8 weeks.
Cyclosporine for more than 8 weeks or during last 8 weeks prior to signing the ICF.
o Voclosporin for more than 8 weeks or during last 8 weeks prior to signing the ICF.
o Belimumab for more than 12 weeks or during last 12 weeks prior to signing the ICF.
8. Receipt of any commercially available Janus kinase inhibitor (eg, tofacitinib or baricitinib) or Bruton’s tyrosine kinase inhibitor (eg, ibrutinib, acalabrutinib, zanubrutinib)
9.Any new medicinal cannabinoid should not be started during the course of the study.
Prior/Concurrent Clinical Study Experience-Participation in another clinical study with another study intervention (besides
anifrolumab) administered within 4 weeks prior to ICF signing or within 5 half-lives of the study intervention used in that clinical study.
Diagnostic Assessments- Within 4 weeks of Week 0 (Day 1), any of the following (note: retesting of laboratory test results during Screening may be repeated once):
AST > 2.5 × ULN
ALT > 2.5 × ULN
TBL > ULN (unless due to Gilbert’s syndrome [based on Investigator’s judgment])
Glycosylated hemoglobin > 8% (or > 0.08) at Screening (diabetic participants only)
Neutrophil count < 1 × 103/μL (or < 1.0 × 109/L)
Platelet count < 25 × 103/μL (or < 25 × 109/L)
Hemoglobin < 8 g/dL (or < 80 g/L)
Other Exclusions- 1. Involvement in the planning and/or conduct of the study (applies to both AstraZeneca staff and/or staff at the study site)
2. Any condition that, in the opinion of the Investigator, would interfere with evaluation of the study intervention or interpretation of participant safety or study results
3. Lactating or pregnant females or females who intend to become pregnant or begin breastfeeding anytime from initiation of Screening through the Follow-up Period
4. weeks following last dose of study intervention and 6 weeks after the last dose of Sponsor-provided MMF (whichever is later) 30 Spontaneous or induced abortion, still or live birth, or pregnancy ≤ 4 weeks prior to signing the ICF
5. Current alcohol, drug or chemical abuse, or a history of such abuse within one year before Week 0 (Day 1)
6. Major surgery within 8 weeks before signing the ICF or elective major surgery planned during the study period
7. History of, or current diagnosis of, catastrophic antiphospholipid syndrome within one year prior to signing the ICF. Participants with other degrees of antiphospholipid syndrome adequately controlled by anticoagulants or aspirin for at least 12 weeks can be recruited to the study.
8. History or evidence of suicidal ideation within the past 6 months; or any suicidal behavior within the past 12 months or recurrent behavior in the lifetime of the participant 
 
Method of Generating Random Sequence   Stratified block randomization 
Method of Concealment   Centralized 
Blinding/Masking   Participant and Investigator Blinded 
Primary Outcome  
Outcome  TimePoints 
Efficacy of anifrolumab compared with placebo as added
to SOC in participants with active proliferative LN on the proportion of participants achieving CRR at Week 52
 
Week 52
 
 
Secondary Outcome  
Outcome  TimePoints 
The efficacy of anifrolumab compared with placebo as added to SOC in
participants with active proliferative LN

 
1. Sustained OCS reduction from Week 24 through Week 52
2. Time to sustained CRR through Week 52
3. Cumulative UPCR through Week 52
4. Time to renal-related event or death through Week 52
5. Time to renal-related event or death through Week 104 
 
Target Sample Size   Total Sample Size="360"
Sample Size from India="30" 
Final Enrollment numbers achieved (Total)= "Applicable only for Completed/Terminated trials"
Final Enrollment numbers achieved (India)="Applicable only for Completed/Terminated trials" 
Phase of Trial   Phase 3 
Date of First Enrollment (India)   15/02/2022 
Date of Study Completion (India) Applicable only for Completed/Terminated trials 
Date of First Enrollment (Global)  10/02/2022 
Date of Study Completion (Global) Applicable only for Completed/Terminated trials 
Estimated Duration of Trial   Years="2"
Months="2"
Days="20" 
Recruitment Status of Trial (Global)
Modification(s)  
Closed to Recruitment of Participants 
Recruitment Status of Trial (India)  Closed to Recruitment of Participants 
Publication Details   NIL 
Individual Participant Data (IPD) Sharing Statement

Will individual participant data (IPD) be shared publicly (including data dictionaries)?  

Response - NO
Brief Summary  

Phase 3 Study of Anifrolumab in Adult Patients with Active Proliferative Lupus Nephritis to evaluate the efficacy and safety of anifrolumab as compared with placebo. The primary and secondary objectives:  1 To evaluate the efficacy of anifrolumab compared with placebo as added to SOC in active proliferative LN on the proportion of participants achieving CRR 2. To evaluate the effect of anifrolumab as compared with placebo as added to SOC on: Sustained OCS reduction, Onset of sustained CRR, Proteinuria, Onset of renal-related event or death through Week 52, Onset of renal-related event or death through Week 104 along with the other efficacy and safety objectives.  A total of 360 participants will be randomized 1:1 to receive anifrolumab or placebo and includes:

Screening Period: Up to 30 days

Treatment Period: 104-week double-blind treatment period with:

Study intervention administration (anifrolumab or placebo) IV Q4W from Week 0 to Week 100 for a total of 26 doses

Follow-up Period: Up to 8 weeks after last efficacy assessment at Week 104 (ie, 12 weeks after last dose of study intervention) 
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