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CTRI Number  CTRI/2021/06/034395 [Registered on: 25/06/2021] Trial Registered Prospectively
Last Modified On: 16/06/2025
Post Graduate Thesis  No 
Type of Trial  Interventional 
Type of Study   Biological 
Study Design  Randomized, Parallel Group, Active Controlled Trial 
Public Title of Study   A comparative clinical study to evaluate efficacy and safety of test drug in patients with Acute Ischemic stroke 
Scientific Title of Study   A prospective, multi-center, randomized, parallel group, two arm, active control, open label, clinical study to evaluate efficacy and safety of Tenecteplase/R-TPR-012 (0.25 mg/kg) compared with Tenectaseâ„¢ (0.20 mg/kg) in patients with Acute Ischemic stroke 
Trial Acronym   
Secondary IDs if Any
Modification(s)  
Secondary ID  Identifier 
RLS/CNS/2020/01 Version 4.0 dated 17 Sep 2021  Protocol Number 
 
Details of Principal Investigator or overall Trial Coordinator (multi-center study)
Modification(s)  
Name  Dr Ajay Kumar Yadav 
Designation  Head - Clinical Research Services 
Affiliation  Reliance Life Sciences Pvt. Ltd. (RLS) 
Address  RLS Bio - Product Trials Group, Dhirubhai Ambani Life Sciences Centre (DALC) R-282, TTC Area of MIDC, Thane - Belapur Road, Rabale, Navi Mumbai - 400701, Thane MAHARASHTRA, India

Thane
MAHARASHTRA
400701
India 
Phone  9820804218  
Fax    
Email  Ajaykumar2.Yadav@relbio.com  
 
Details of Contact Person
Scientific Query

Modification(s)  
Name  Dr Ajay Kumar Yadav 
Designation  Head Clinical Research 
Affiliation  Reliance Life Sciences Pvt. Ltd. (RLS) 
Address  RLS Bio - Product Trials Group, Dhirubhai Ambani Life Sciences Centre (DALC) R-282, TTC Area of MIDC, Thane - Belapur Road, Rabale, Navi Mumbai - 400701, Thane MAHARASHTRA, India

Thane
MAHARASHTRA
400701
India 
Phone  9820804218  
Fax    
Email  Ajaykumar2.Yadav@relbio.com  
 
Details of Contact Person
Public Query

Modification(s)  
Name  Ganesh Bagul 
Designation  Head - Clinical Operations 
Affiliation  Reliance Life Sciences Pvt. Ltd. (RLS) 
Address  RLS Bio - Product Trials Group, Dhirubhai Ambani Life Sciences Centre (DALC) R-282, TTC Area of MIDC, Thane - Belapur Road, Rabale, Navi Mumbai - 400701, Thane MAHARASHTRA, India

Thane
MAHARASHTRA
400701
India 
Phone  9820617721  
Fax    
Email  ganesh1.bagul@relbio.com  
 
Source of Monetary or Material Support  
Reliance Life Sciences Pvt Ltd., Dhirubhai Ambani Life Sciences Centre (DALC) R-282, TTC Area of MIDC, Thane - Belapur Road, Rabale, Navi Mumbai - 400701, Maharashtra, India 
 
Primary Sponsor  
Name  Reliance Life Sciences Pvt Ltd 
Address  Dhirubhai Ambani Life Sciences Centre (DALC), R-282, TTC Area of MIDC, Rabale, Navi Mumbai – 400701, Maharashtra, India 
Type of Sponsor  Pharmaceutical industry-Indian 
 
Details of Secondary Sponsor  
Name  Address 
NIL  NIL 
 
Countries of Recruitment     India  
Sites of Study
Modification(s)  
No of Sites = 17  
Name of Principal Investigator  Name of Site  Site Address  Phone/Fax/Email 
Dr Sunit Shah  Advance Neurology & Superspeciality hospital  D-357/358, GT Marg, Malavia Nagar, Jaipur-302017, Rajasthan
Jaipur
RAJASTHAN 
9252546364

advanceneuroresearch@gmail.com 
Dr Sanjeev Kumar Bhoi  All India Institute of Medical Sciences, Bhubaneswar, Odisha  All India Institute of Medical Sciences, Sijua, Patrapada, Dumduma, Bhubaneswar, Odisha, Pin-751019
Khordha
ORISSA 
06742476789

director@aiimsbhubaneswar.edu.in 
Dr S Meenakshi Sundaram  Apollo Speciality Hospitals  Lake View road, K.K. Nagar, Madurai, Tamilnadu, India -625020
Madurai
TAMIL NADU 
9842980211

drsundarsms@gmail.com 
Dr Rajaram Agarwal  Asopa Hospital  93B, Tagore nagar, Near DCM, Ajmer Road, Jaipur-302021, India
Jaipur
RAJASTHAN 
9196673481

advanceneuroresearch@gmail.com 
Dr Amit Yeole  Chopda Medical & Research Centre Pvt.Ltd, magum Heart Institute  3/5, patil Lane no 1, Laxmi Nagar, Near K.B.H, Vidyalaya, Canada Corner, nashik, 422005, Maharastra India
Nashik
MAHARASHTRA 
7588554530

amit_yeole37@rediffmail.com 
Dr Varoon Vadodaria  Dhiraj Hospital, Sumandeep Vidyapeeth  Department of Neurology Dhiraj Hospital Sumandeep Vidyapeeth Deemed to be University Piparia
Vadodara
GUJARAT 
9714430239

vadodariavaroonj@gmail.com 
Dr Pradeep Tiwari  Dr. D.Y Patil Medical College, Hospital & Research Centre  Dr. D.Y Patil Medical College, Hospital & Research Centre, Sector 5, Nerul, Navi Mumbai - 400706, Maharashtra, India
Mumbai
MAHARASHTRA 
9131973517

drtiwarippt16@gmail.com 
Dr Ranjana Deshmukh  Dr. Hegdevar Hospital  Garkheda, Aurangabad-431005, India
Aurangabad
MAHARASHTRA 
02402245000

ranjana-deshmukh@hedgewar.org 
Dr TCR Ramakrishnan  KG Hospital & Post Graduate Medical institute & Research  First Floor B Block, Department of Neurology,Centre, No-5, Government arts college road, Coimbatore, Tamilnadu India, 641018
Coimbatore
TAMIL NADU 
94433365792

kgneuro@gmail.com 
Dr Kunal Jadhav  Lifepoint Multispecialty Hospilal  145/1, Mumbai Bangalore Highway, Near Hotel Sayaji,Wakad,Pune-411057,Maharashtra,lndia.
Pune
MAHARASHTRA 
020-66434346

jadhavkunal363@gmail.com 
Dr Amit Pande  Medipoint Hospital Pvt. Ltd  241/1, New D.P. Road, Aundh Pune-411007, Maharastra India
Pune
MAHARASHTRA 
9860918000

dramitkumarpande.pentagon@gmail.com 
Dr Pandurang Wattamwar  Oriion Citicare Super Speciality Hospital  Department of Neurology, 5-5-70, Opposite Kalash Mangal Karyalaya, New Osmanpura, Aurangabad - 431005, Maharashtra, India
Aurangabad
MAHARASHTRA 
7387013361

drpandu1976@gmail.com 
Dr Jagdish Kookna  S P Medical College & Associated Group of Hospitals  Department of Neurology, S.P Medical College & AG of Hospitals, Bikaner, Rajasthan, India . Pin 334003
Bikaner
RAJASTHAN 
91-151-2226300
91-151-2226301
drjckookna@gmail.com 
Dr Ashutosh Rath  Shanti Memorial Hospital  Shanti Memorial Hospital, THoria Sahi, Patnaik COlony, Mangalabaug, Cuttack-753001, Odisha
Cuttack
ORISSA 
9937737986

rathashutosh666@gmail.com 
Dr Rahul Baviskar  Shree Siddhivinayak Maternity & Nursing home   2nd floor, Department of Neurology,Unity Campus, Opp. KTHM College, gangapur Road, Nasik 422002, Maharastra India
Nashik
MAHARASHTRA 
2532580470

rbaviskar@gmail.com 
Dr Dinesh Khandelwal  SMS Medical College & Hospital  Room No o6, Ground floor, Dept of Neurology, Dhanvantari OPD Block SMS Hospital, Jaipur-302004 rajasthan India
Jaipur
RAJASTHAN 
9413341485

drdineshkhandelwal@gmail.com 
Dr Boopathy Rangappan Munirathinam  Tamil Nadu Government multi super speciality hospital  Omandurar Government Estate Opp The Hindu Mount Road Anna Salai Chennai Tamil Nadu 600002
Chennai
TAMIL NADU 
840051256

drbhoopathy.tngmssh@gmail.com 
 
Details of Ethics Committee
Modification(s)  
No of Ethics Committees= 13  
Name of Committee  Approval Status 
Asopa Ethics Committee, Asopa Hospital, 93 B, tagore Nagar, Near DCM, Ajmer Road, Jaipur India  Approved 
Asopa Ethics Committee, Asopa Hospital, 93 B, tagore Nagar, Near DCM, Ajmer Road, Jaipur India  Approved 
Dr. BAMRS Dr. Hegdewar Hiospital Ethics Committee, Dr. Hegdevar Hospital , Garkheda, Aurangabad-431005, India  Approved 
Ethics Committee SMS Medical College & Attached Hospital, J.L.N marg, Jaipur rajasthan, 302004  Approved 
Ethics Committee, S P Medical College, Bikaner  Approved 
Institutional Ethics Committee D Y Patil Medical College  Submittted/Under Review 
Institutional Ethics committee, K.G Hospital, K Govindaswami Naidu Medical trust  Approved 
Institutional Ethics Committee-Clinical StudiesApollo Speciality Hospital, Lake View road, K K Nagar, Madurai -625020  Approved 
LPR Ethics Committee Lifepoint Multispecialty Hospital, 145/1, Mumbai Bangalore Highway, Near Hotel Sayaji, Wakad,Pune-411057,Maharashtra,lndia.  Approved 
Manga care Ethics Committee, C/o Chopda Medical & Research Centre Pvt.Ltd, magum Heart Institute, 3/5, patil Lane no 1, Laxmi Nagar, Near K.B.H, Vidyalaya, Canada Corner, nashik, 422005, Maharastra India  Approved 
Oriion Citicare Hospital Institutional EC, ORIION Citicare Superspeciality Hospital, Opp. Kalash Mangal Karyalay, New Osmanpura Kranti Chowk, Aurangabad, Maharashtra - 431005, India  Approved 
Penta-Med Ethics Committee   Medipoint Hospitals Pvt. Ltd   Medipoint Hospitals Pvt.Ltd 241/1, New D.P.Road,   Near Sai Heritage,Aundh Pune Pune Maharashtra -   411007 India   Approved 
Shree Siddhivinayak Hospital Ethics Committee, C/O Shree Siddhivinayak Maternity & Nursing home ,Unity Campus, Opp. KTHM College, gangapur Road, Nasik 422002, Maharastra India.  Approved 
 
Regulatory Clearance Status from DCGI
Modification(s)  
Status 
Approved/Obtained 
 
Health Condition / Problems Studied  
Health Type  Condition 
Patients  (1) ICD-10 Condition: G00-G99||Diseases of the nervous system,  
 
Intervention / Comparator Agent  
Type  Name  Details 
Comparator Agent  Tenectaseâ„¢(0.20 mg/kg)  Tenectase will be administered at a dose of 0.20mg/kg – maximum 20 mg as per the prescribing information of the drug at Day 1. 
Intervention  Tenecteplase/R-TPR-012 (0.25 mg/kg)  R-TPR-012 will be administered at a dose of 0.25mg/kg - maximum 25 mg as a single intravenous bolus over approximately 10 seconds at Day 1. 
 
Inclusion Criteria
Modification(s)  
Age From  18.00 Year(s)
Age To  75.00 Year(s)
Gender  Both 
Details  1. Men or women aged 18 to 75 years, inclusive.
2. Patients with acute ischemic stroke as per the pre-treatment CT and NIH stroke scale and
eligible for IV thrombolysis.
3. Patients presenting in the hospital and eligible to receive TenectaseTM as per the
prescribing information.
OR
Patients presenting in the hospital <4.5 hours from the onset of symptoms.
4. Consent from Legally Acceptable Representative (LAR) would be obtained, if patient is
not in the condition to give consent. However, when the patient is stable and is able to
give consent, consent would be obtained to confirm his/her willingness to continue in the
study.
5. Patient independent prior to the stroke (estimated modified Rankin Scale 0-1). 
 
ExclusionCriteria 
Details  1. Evidence findings on pre-treatment CT that indicate that the patient is unlikely to benefit
from treatment:
A) Infarction comprising more than >1/3 of the middle cerebral artery territory and ASPECTS score of ≤ 7
B)Intracranial haemorrhage, structural brain lesions which can mimic stroke (e.g.
cerebral tumour)
2. Hypodense lesion on pre-treatment CT consistent with recent cerebral ischaemia other than the presenting event.
3. Large areas (greater than one lobe) of obvious low density on baseline head CT scan.
4. Rapidly improving or minor acute ischemic stroke symptoms
5. Subjects with Positive COVID antigen test
6. Systolic BP > 180 or diastolic BP > 110 mmHg, or aggressive management (intravenous pharmacotherapy) necessary to reduce BP below these limits
7. Clinical history suggestive of subarachnoid haemorrhage even if no blood is evident on
CT scan
8. Active internal bleeding except menstruation
9. Patients with severe hypoglycaemia (blood glucose <50mg/dL) or severe hyperglycaemia
(blood glucose >400 mg/dL) sufficient to account for neurological symptoms
10. Seizure at onset of symptoms unless brain imaging identifies positive evidence of significant brain ischaemia (e.g. Early ischaemic change or hyperdense vessel on plain CT or computerised tomography angiography (CTA) scan confirmed arterial occlusion)
11. Patients taking warfarin and INR > 1.7
12. Patients taking a direct oral anticoagulant (dabigatran, rivaroxaban, apixaban, edoxaban)
unless the last dose was taken more than 12 hrs prior to screening and along with normal
coagulation assays
13. Low molecular weight heparins (LMWH) (at doses other than prophylaxis of venous
thromboembolism) administered within the preceding 48 hours, Unfractionated heparin
administered within the previous 48 hours and aPTT is prolonged
14. Significant non-stroke intracranial pathology likely to account for clinical presentation or
represent a risk of intracerebral haemorrhage (e.g.,CNS neoplasm) on pre-treatment CT
15. More than one stroke episode within the previous 14 days prior to screening
16. Thrombolytic therapy within the previous 14 days prior to screening
17. History of Intracranial neoplasm or aneurysm
18. Myocardial infarction within 30 days prior to screening
19. Intracranial or intraspinal surgery or intracranial trauma within past 2 months
20. History of arteriovenous maltransformation
21. Patients with high risk of haemorrhage including history of major surgery or major
trauma within 21 days prior to screening
22. Patient with history of gastrointestinal or urinary tract haemorrhage within 21 days prior
to screening
23. Arterial puncture at a non-compressible site within 7 days prior to screening
24. Prolonged cardiopulmonary resuscitation (>2min) within 14 days prior to screening
25. Current acute pericarditis and/or sub-acute bacterial endocarditis
26. Patients with acute pancreatitis
27. History of severe hepatic dysfunction, including hepatic failure, cirrhosis, portal
hypertension (oesophageal varices) and active hepatitis
28. History of active peptic ulceration
29. Known history of haemorrhagic stroke
30. Patients with known bleeding diathesis and/or platelet count <100 000/mm3.
31. Acute endovascular treatment for stroke is planned.
32. Patients with known history of HIV, HBsAg or HCV.
33. Any major medical condition that is likely to limit survival to Day 90.
34. Pregnancy or lactation, or parturition within the previous 30 days. Women of childbearing
potential must have a negative pregnancy test.
35. Participation in any clinical study of an investigational product within previous 3 months.
36. Current signs or symptoms of significant, progressive or uncontrolled renal, hepatic,
hematologic, gastrointestinal, endocrine, pulmonary, or cardiac disease that renders the patient incapable of participating in the study.
37. History of other disease, active systemic infection, metabolic dysfunction, physical
examination finding, or clinical laboratory finding giving reasonable suspicion of a
disease or condition that contraindicates the use of an investigational drug or that might
affect interpretation of the results of the study or render the subject at high risk for
treatment complications.
38. Any other condition which the investigator feels would pose a significant hazard to
patient if tenecteplase is administered.
39. Patient unlikely to complete Day 90 follow-up 
 
Method of Generating Random Sequence   Computer generated randomization 
Method of Concealment   An Open list of random numbers 
Blinding/Masking   Not Applicable 
Primary Outcome  
Outcome  TimePoints 
Mean change in the modified Rankin Scale (mRS)  at Day 90 
 
Secondary Outcome  
Outcome  TimePoints 
Full neurological recovery defined as mRS score of 0-1  at Day 90 
Independent recovery defined as mRS score 0-2  at Day 90 
Early major neurological improvement from baseline NIHSS total score  at 24 hours 
Change from baseline in Health Related Quality of Life  at Day 7, Day 30 and Day 90 
Change from baseline in Barthel Index score  at Day 7, Day 30 and Day 90 
All-cause Mortality  at Day 90 
Incidence of Symptomatic Intra-Cerebral Haemorrhage (SICH)  up to Day 90 
Incidence of Parenchymal Haematoma type 2 (PH2) haemorrhage based on European
Cooperative Acute Stroke Study (ECASS) II on post-treatment CT scan  
up to 36 hours after study drug administration. 
Incidence of intracranial haemorrhage  within 36 hours of study drug administration on
CT scan 
Incidence of significant extra-cranial haemorrhage (requirement for blood transfusion or drop in haemoglobin of ≥2.0mg/dL)  within 36 hours of study drug administration 
Immunogenicity assessment  at baseline, Day 7, Day 30 and at safety follow up visit (Day 90) or at withdrawal visit 
 
Target Sample Size   Total Sample Size="225"
Sample Size from India="225" 
Final Enrollment numbers achieved (Total)= "Applicable only for Completed/Terminated trials"
Final Enrollment numbers achieved (India)="Applicable only for Completed/Terminated trials" 
Phase of Trial   Phase 2/ Phase 3 
Date of First Enrollment (India)
Modification(s)  
30/04/2022 
Date of Study Completion (India) Applicable only for Completed/Terminated trials 
Date of First Enrollment (Global)  Date Missing 
Date of Study Completion (Global) Applicable only for Completed/Terminated trials 
Estimated Duration of Trial   Years="0"
Months="8"
Days="0" 
Recruitment Status of Trial (Global)
Modification(s)  
Not Applicable 
Recruitment Status of Trial (India)  Open to Recruitment 
Publication Details   NIL 
Individual Participant Data (IPD) Sharing Statement

Will individual participant data (IPD) be shared publicly (including data dictionaries)?  

Response - NO
Brief Summary  

This is a phase II/III, prospective, multi-center, open label, non-inferiority, two-arm, parallel group, active control, randomized, comparative clinical study to evaluate efficacy, safety and immunogenicity of R-TPR-012 (0.25 mg/kg) with Tenectase (0.20 mg/kg) in patients with acute ischemic stroke.

 
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