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CTRI Number  CTRI/2021/04/032837 [Registered on: 15/04/2021] Trial Registered Prospectively
Last Modified On: 08/02/2022
Post Graduate Thesis  Yes 
Type of Trial  Interventional 
Type of Study   Drug 
Study Design  Single Arm Study 
Public Title of Study   A clinical trial to study the safety and efficacy of PEGylated Factor VIII (BAX 855) in previously untreated patients (PUPs) 6 years with severe hemophilia A (FVIII 1%) 
Scientific Title of Study   Phase 3, prospective, multi-center, open label study to investigate safety, immunogenicity, and hemostatic efficacy of PEGylated Factor VIII (BAX 855) in previously untreated patients (PUPs) less than 6 years with severe hemophilia A (FVIII less than 1%) 
Trial Acronym  BAX855 PUP  
Secondary IDs if Any  
Secondary ID  Identifier 
Protocol no 261203 version 3.0 dated 26 Nov 2015  Protocol Number 
 
Details of Principal Investigator or overall Trial Coordinator (multi-center study)  
Name   
Designation   
Affiliation   
Address 




 
Phone    
Fax    
Email    
 
Details of Contact Person
Scientific Query
 
Name  Dr Inderjeet Singh 
Designation  Clinical Development Lead Takeda India 
Affiliation  Baxalta Bioscience India Pvt. Ltd.  
Address  6th Floor Tower C Building No 8 DLF Cyber City DLF Phase-II Gurgaon 122 002 Haryana India

Gurgaon
HARYANA
122002
India 
Phone  8146095015  
Fax    
Email  inderjeet.singh@takeda.com  
 
Details of Contact Person
Public Query
 
Name  Anoop Singh 
Designation  Study Site Engagement Lead SSEL APAC & ICMEA Clinical Development Services Takeda India  
Affiliation  Baxalta Bioscience India Pvt. Ltd.  
Address  6th Floor Tower C Building No 8 DLF Cyber City DLF Phase-II Gurgaon 122 002 Haryana India

Gurgaon
HARYANA
122002
India 
Phone  8105536000  
Fax    
Email  anoop.singh@takeda.com  
 
Source of Monetary or Material Support  
Baxalta Innovations GmbH Industriestrasse 67 A-1221 Vienna, Austria  
 
Primary Sponsor  
Name  Baxalta Innovations GmbH  
Address  Industriestrasse 67 A-1221 Vienna, Austria  
Type of Sponsor  Contract research organization 
 
Details of Secondary Sponsor  
Name  Address 
Baxalta Bioscience India Pvt Ltd  Baxalta Bioscience India Pvt. Ltd. 6th Floor, Tower-C, Building No.8, DLF Cyber City, DLF Phase-II, Gurgaon-122 002, Haryana, India Tel: 0124-4559100  
 
Countries of Recruitment     India  
Sites of Study  
No of Sites = 7  
Name of Principal Investigator  Name of Site  Site Address  Phone/Fax/Email 
Dr Neeraj Sidharthan  Amrita Institute of Medical Sciences and Research Centre  Ponekkara, Edappally, Ernakulam, Kochi -682041, Kerala, India
Ernakulam
KERALA 
9946047464

neerajsidharthan@aims.amrita.edu 
Dr Fauzia Nambiathayil Aboobacker  Christian Medical College  Ida Scudder Road, Vellore- 632004, Tamil Nadu, India
Vellore
TAMIL NADU 
91-416-2282352

fouzian@cmcvellore.ac.in 
Dr Priyanka Samal  Institute of Medical Sciences and SUM Hospital  K8 Kalinga Nagar, K8 Kalinga Nagar, Shampur, Bhubaneswar, Odisha 751003, India, Bhubaneswar, Odisha 751003
Sonapur
ORISSA 
8902621993

samalpriyanka80@gmail.com 
Dr Nirmal Ganeshmal Choraria  Nirmal Hospital Pvt Ltd  2/1423-8-6 Sagrampura, Ring Road, Near Centre Point, Surat-395002, Gujarat, India
Surat
GUJARAT 
9825142549

drnirmalchoraria@gmail.com 
Dr Prakas Mandal  NRS Medical College  Centenary building, 4th floor, 138-A, J. C. Bose road, Kolkata-700014
Kolkata
WEST BENGAL 
9433345001

prakas70@gmail.com 
Dr Shashikant Janardan Apte  Sahyadri Super Specialty Hospital  Plot No. 30C, Erandawane, Karve Road, Pune-411004, Maharashtra, India.
Pune
MAHARASHTRA 
9822404983

shashikant.apte@gmail.com 
Dr Cecil Reuben Ross  St Johns Medical College Hospital  Sarjapur Road, Koramangala, Bengaluru (Bangalore) Urban- 560034, Karnataka, India.
Bangalore
KARNATAKA 
9448493705

cecilrross@gmail.com 
 
Details of Ethics Committee
Modification(s)  
No of Ethics Committees= 7  
Name of Committee  Approval Status 
Ethics Committee, N.R.S. Medical College NRS Medical College And Hospital 138, A.J.C Bose Road, Kolkata 700014,West Bengal India  Approved 
Institutional Ethics Committee, Amrita Institute of Medical Sciences AIMS-Ponekkara, Edappally, Ernakulam, Kochi -682041, Kerala, India.  Approved 
Institutional Ethics Committee, Institute of Medical Sciences and SUM Hospital, SIKSHA ‘O’ ANUSANDHAN (Deemed to be University), Sector-8, Kalinga Nagar, Ghatikia, Bhubaneshwar-751003, Odisha, India  Approved 
Institutional Ethics Committee, St. Johns Medical College Hospital, Sarjapur Road, Koramangala, Bengaluru (Bangalore) Urban- 560034, Karnataka, India.  Submittted/Under Review 
INSTITUTIONAL REVIEW BOARD CHRISTIAN MEDICAL COLLEGE THORAPADI POST BAGAYAM VELLORE Tamil Nadu -632012,India  Approved 
Nirmal Hospital Ethics Committee, Nirmal Hospital Pvt. Ltd., 2/1423-8-6 Sagrampura, Ring Road, Near Centre Point, Surat-395002, Gujarat, India.  Approved 
Sahyadri Hospitals Ltd. Ethics Committee, Sahyadri Clinical Research & Development Center (A Unit of Sahyadri Hospitals Ltd.), 33/34B, Makarand Bhave Path, Karve Road, Pune- 411004, Maharashtra, India  Approved 
 
Regulatory Clearance Status from DCGI
Modification(s)  
Status 
Approved/Obtained 
 
Health Condition / Problems Studied  
Health Type  Condition 
Patients  (1) ICD-10 Condition: D688||Other specified coagulation defects,  
 
Intervention / Comparator Agent  
Type  Name  Details 
Comparator Agent  Not applicable  Not applicable 
Intervention  Single arm with 2 sub-arms (surgery, ITI)  PEGylated Recombinant Factor VIII Polyethylene glycol (PEG)-ylated full-length recombinant FVIII (rFVIII) Dosage form: injection, powder, lyophilized, for solution Part A (main study): On-demand: 10-80 IU/kg depending on the severity of the bleeding episode. Subjects may start with on-demand therapy if they are younger than 3 years and if they have not experienced two joint bleeds. Prophylaxis: To be initiated before the age of 3 years or after a maximum of 2 joint bleeds, whichever occurs first, at a dose of 25-80 IU/kg at least once weekly. Surgery (Part A): The dose and frequency of BAX 855 administered will be individualized based on the subject’s BAX 855 IR and half-life, if available, to obtain the target level required for the type of the surgery, dental or other invasive procedure being performed. In general, for major surgery initial FVIII target levels in plasma should be ≥ 80-100% of normal FVIII level, whereas for minor surgery target FVIII levels should be ≥ 30-60%. Part B (ITI): daily 100-200 IU/kg or three times weekly 50 IU/kg. In case of success, high-dose ITI regimen, if applicable, will be reduced to twice weekly prophylaxis as follows: ï‚· 100±5 IU/kg/day for the first four weeks; then ï‚· 50±5 IU/kg/day for a further four weeks; then ï‚· 50±5 IU/kg every second day for a further four weeks; then ï‚· 50±5 IU/kg administered twice a week, adjusted as needed to maintain a FVIII trough level of 1%, for a further 3 months. Subjects receiving low dose ITI therapy with 3 x 50 IU/kg weekly will be transitioned to twice weekly 50 IU/kg, adjusted as needed to maintain a FVIII trough level of 1%, for a further 3 months. After this period, a Completion/Termination Visit will be performed. Mode of Administration: intravenous bolus 
 
Inclusion Criteria  
Age From  1.00 Year(s)
Age To  6.00 Year(s)
Gender  Both 
Details  1.Subject is < 6 years old at the time of screening 2. Subject is previously untreated with < 3 EDs to ADVATE, BAX 855 or Fresh Frozen Plasma
(FFP) at any time prior to screening 3.
Subject has severe hemophilia A (FVIII < 1%) as determined by the central laboratory, or a historical FVIII level < 1% as determined at any local laboratory, optionally supported by an additional FVIII gene mutation consistent with severe hemophilia A 4.
Subject is immune competent with a CD4+ count > 200 cells/mm3, as confirmed by central laboratory at screening 5. Parent or legally authorized representative is willing and able to comply with the requirements of the protocol

Additional inclusion criteria for Part B (ITI): 1 Parent or legal representative has/have voluntarily provided signed informed consent for ITI portion 2 Subject has a confirmed positive high titer inhibitor (> 5.00 BU) or has a positive confirmed low titer inhibitor (≥ 0.6 BU) as determined by the central laboratory based on a second repeat blood sample with a) poorly controlled bleeding despite increased BAX 855 doses, or b) requires bypassing agents to treat bleeding episodes
 
 
ExclusionCriteria 
Details  1 Subject has detectable FVIII inhibitory antibodies (≥0.6 BU using the Nijmegen modification of the Bethesda assay) as confirmed by central laboratory at screening
2 S2. Subject has a history of FVIII inhibitory antibodies (≥ 0.6 BU using the Nijmegen modification of the Bethesda assay or the Bethesda assay) at any time prior to screening
3 Subject has been diagnosed with an inherited or acquired hemostatic defect other than
hemophilia A (eg, qualitative platelet defect or von Willebrand’s disease)
4 Subject has been previously treated with cryoprecipitate or any type of FVIII concentrate other
than ADVATE, BAX 855v or FFP, or was administered ADVATE,BAX 855 or FFP for ≥ 3 Eds at any time prior to screening.
5 Subject has received any kind of blood-transfusion such as PRBC, platelets or plasma prior to
screening at any time prior to screening
6 The subject’s weight is < 5 kg
7 Subject’s platelet count is < 100,000/mL
8 Subject has known hypersensitivity towards mouse or hamster proteins, PEG or Tween 80
9 Subject has severe chronic hepatic dysfunction [eg, > 5 times upper limit of normal alanine
aminotransferase (ALT), aspartate aminotransferase (AST), or a documented INR > 1.5] in his
medical history or at the time of screening
10 Subject has severe renal impairment (serum creatinine > 1.5 times the upper limit of normal)
11 Subject has current or recent (< 30 days) use of other PEGylated drugs prior to study participation or is scheduled to use such drugs during study participation
1212. Subject is scheduled to receive during the course of the study a systemic immunomodulating
drug (e.g. corticosteroid agents at a dose equivalent to hydrocortisone greater than 10 mg/day, or
α-interferon) other than anti-retroviral chemotherapy
13 Subject has participated in another clinical study involving an IP or investigational device within 30 days prior to enrollment or is scheduled to participate in another clinical study involving an IP or investigational device during the course of this study
14 14. Parent or legally authorized representative has a medical, psychiatric, or cognitive illness or recreational drug/alcohol use that, in the opinion of the investigator, would affect subject safety or compliance
15 Parent, legally authorized representative or subject are a member of the team conducting this
study or is in a dependent relationship with one of the study team members. Dependent
relationships include close relatives (ie, children, partner/spouse, siblings, parents) as well as
employees of the investigator or site personnel conducting the study.

Additional exclusion criteria for Part B (ITI)
1 Spontaneous disappearance of the inhibitor prior to ITI
2 FVIII inhibitor titer ≥ 0.6 BU is not confirmed by a second new blood sample drawn within
2 weeks of study site notification of inhibitor and determined at the central laboratory
3 Inability or unwillingness to comply with the protocol 
 
Method of Generating Random Sequence   Computer generated randomization 
Method of Concealment   Centralized 
Blinding/Masking   Open Label 
Primary Outcome  
Outcome  TimePoints 
Incidence of FVIII inhibitor development Success rate of Immune tolerance induction (ITI)  Throughout Part A of the study, approximately 5 years
Up to 33 months
 
 
Secondary Outcome  
Outcome  TimePoints 
Safety:• Binding IgG and IgM antibodies to FVIII, PEG-FVIII and PEG
• AEs and SAEs
• Clinically significant changes in vital signs and clinical laboratory parameters (hematology and clinical chemistry) 
Throughout Part A of the study, approximately 5 years
Throughout Part A and Part B of the study, approximately 7 years
Throughout Part A and Part B of the study, approximately 7 years 
Efficacy •Annualized bleeding rate (ABR) for prophylactic and on-demand treatment
•Number of BAX 855 infusions per bleeding episode
•Overall hemostatic efficacy rating at 24 h after initiation of treatment and at resolution of bleed
•Weight-adjusted consumption of BAX 855 per month, per year and per event (prophylaxis, treatment of bleeding episode and surgery) and the number of infusions per month and per year

 

Throughout Part A of the study, approximately 5 years
24 h after initiation of treatment and at resolution of bleed

Surgery Day 0 up to postoperative Day 14 or discharge (whichever occurs first)
Surgery Day 0 up to postoperative Day 14 or discharge (whichever occurs first)
Pre-infusion within 30 minutes; and post-infusion at 15-30 minutes and 24-48 hours
ost-infusion: 15-30 minutes and 24-48 hours 
• Assessment of intra-, post- and perioperative hemostatic efficacy in case of surgery
• Intra- and postoperative blood loss in case of surgery
• IR at baseline and over time
• Half-life at baseline (optional). This is based on an abbreviated PK using 2 post-infusion timepoints: 15-30 minutes and 24–48 hours 
Surgery Day 0 up to postoperative Day 14 or discharge (whichever occurs first
Surgery Day 0 up to postoperative Day 14 or discharge (whichever occurs first)
Pre-infusion within 30 minutes; and post-infusion at 15-30 minutes and 24-48 hours  
Additional Outcome Measures for ITI: Primary: The success rate of ITI therapy with BAX 855, success being defined as 1) a persistently negative inhibitor titer 0.6 BU (confirmed by central laboratory with second blood specimen obtained within 2 months); 2) a FVIII IR 66% of baseline value following a wash-out period of
84-96 h, and 3) a FVIII half-life of ≥ 6 hours.

 
If no baseline IR is available, the IR value is indicative of an adequate clinical response following a wash-out period of 84-96 h. 
•ABR during ITI
•Weight-adjusted consumption of BAX 855 per month and per year for each ITI regimen employed
•Catheter-related complications
•Binding IgG and IgM antibodies to FVIII, PEG-FVIII, and PEG
•The rate of partial success and failure of ITI with BAX 855  
Up to 33 months  
 
Target Sample Size   Total Sample Size="12"
Sample Size from India="12" 
Final Enrollment numbers achieved (Total)= "Applicable only for Completed/Terminated trials"
Final Enrollment numbers achieved (India)="Applicable only for Completed/Terminated trials" 
Phase of Trial   Phase 3 
Date of First Enrollment (India)   15/04/2021 
Date of Study Completion (India) Applicable only for Completed/Terminated trials 
Date of First Enrollment (Global)  Date Missing 
Date of Study Completion (Global) Applicable only for Completed/Terminated trials 
Estimated Duration of Trial   Years="5"
Months="5"
Days="0" 
Recruitment Status of Trial (Global)
Modification(s)  
Not Applicable 
Recruitment Status of Trial (India)  Closed to Recruitment of Participants 
Publication Details   Not applicable 
Individual Participant Data (IPD) Sharing Statement

Will individual participant data (IPD) be shared publicly (including data dictionaries)?  

Response - NO
Brief Summary   This study is a Phase 3, prospective, open-label, multicenter study to assess the safety, immunogenicity and hemostatic efficacy of BAX 855 in PUPs < 6 years of age with severe hemophilia A (baseline FVIII level < 1%) and < 3 EDs to ADVATE, BAX 855viii or plasma in at least 100 evaluable subjects. Subjects will receive prophylactic and/or on demand therapy with BAX 855 for at least 100 EDs or until they have developed a confirmed FVIII inhibitor (Part A). During Part A, subjects can undergo surgical or invasive procedures under a protocol-defined regimen for BAX 855. Subjects who develop a high titer FVIII inhibitor or subjects with low titer FVIII inhibitors where ITI therapy is necessary because of poorly controlled bleeding despite increased BAX 855 doses or bypassing agents are required to treat bleeding may enter Part B (ITI portion) of the study to undergo ITI therapy with BAX 855. 
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