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CTRI Number  CTRI/2021/03/032140 [Registered on: 19/03/2021] Trial Registered Prospectively
Last Modified On: 20/07/2022
Post Graduate Thesis  No 
Type of Trial  Interventional 
Type of Study   Drug 
Study Design  Randomized, Crossover Trial 
Public Title of Study   A bioequivalence study of Efonidipine 40 mg and Chlorthalidone 12.5 mg tablet combination in healthy adults. 
Scientific Title of Study   An open label, balanced, randomized, two-treatment, two-sequence, two-period, single-dose, truncated (only for chlorthalidone) crossover oral bioequivalence study of test product, fixed dose combination of Efonidipine HCl ethanolate 40 mg and Chlorthalidone 12.5 mg tablets of Zuventus Healthcare Limited, India with reference products, Efnocar 40 (Efonidipine HCl ethanolate 40 mg) tablets, manufactured by Zuventus Healthcare Limited, India and CTD®-12.5 (Chlorthalidone 12.5 mg) tablets, manufactured by Ipca Laboratories Limited, India in normal, healthy, adult, human subjects under fasting conditions. 
Trial Acronym   
Secondary IDs if Any  
Secondary ID  Identifier 
20-242 (Ver No. 01, dated: 03.11.2020)  Protocol Number 
 
Details of Principal Investigator or overall Trial Coordinator (multi-center study)  
Name  Dr Bhupesh Dewan 
Designation  Director- Medical Services 
Affiliation  Zuventus Healthcare Limited 
Address  3101, Department of Medical Services, C wing, Oberoi Garden Estate, Chandivali, Andheri (E), Mumbai Mumbai MAHARASHTRA 400072 India

Mumbai
MAHARASHTRA
400072
India 
Phone  022-30610000  
Fax    
Email  Bhupesh.Dewan@zuventus.com  
 
Details of Contact Person
Scientific Query
 
Name  Dr Suraj Kumar K 
Designation  Principal Investigator 
Affiliation  Jeevan Scientific Technology Ltd 
Address  Clinical Pharmacology Center, Clinical Department, Clinical Research Division, 4th Floor, B-17, TIE, Phase II, Balanagar, Hyderabad

Hyderabad
TELANGANA
500037
India 
Phone  040-67364700  
Fax    
Email  suraj.katpali@jeevanscientific.com  
 
Details of Contact Person
Public Query
 
Name  Dr Bhupesh Dewan 
Designation  Director- Medical Services 
Affiliation  Zuventus Healthcare Limited 
Address  3101, Department of Medical Services, C wing, Oberoi Garden Estate, Chandivali, Andheri (E), Mumbai Mumbai MAHARASHTRA 400072 India

Mumbai
MAHARASHTRA
400072
India 
Phone  022-30610000  
Fax    
Email  Bhupesh.Dewan@zuventus.com  
 
Source of Monetary or Material Support  
Zuventus Healthcare Limited, 5119, D Wing, Oberoi Garden Estate, Chandivali, Andheri (E), Mumbai- 400072 
 
Primary Sponsor  
Name  Zuventus Healthcare Limited 
Address  5119, D Wing, Oberoi Garden Estate, Chandivali, Andheri (E), Mumbai- 400072 
Type of Sponsor  Pharmaceutical industry-Indian 
 
Details of Secondary Sponsor  
Name  Address 
NIL  NIL 
 
Countries of Recruitment     India  
Sites of Study  
No of Sites = 1  
Name of Principal Investigator  Name of Site  Site Address  Phone/Fax/Email 
Dr Suraj Kumar K  Jeevan Scientific Technology Ltd.  Clinical Pharmacology Center, Clinical Department, Clinical Research Division, 4th Floor, B-17, TIE, Phase II, Balanagar Hyderabad
Hyderabad
TELANGANA 
4067364700

suraj.katpali@jeevanscientific.com 
 
Details of Ethics Committee  
No of Ethics Committees= 1  
Name of Committee  Approval Status 
Vasavi Medical & Research Centre  Approved 
 
Regulatory Clearance Status from DCGI  
Status 
Approved/Obtained 
 
Health Condition / Problems Studied  
Health Type  Condition 
Healthy Human Volunteers  Normal, healthy, adult, human subjects under fasting conditions. 
 
Intervention / Comparator Agent  
Type  Name  Details 
Comparator Agent  CTD®-12.5 (Chlorthalidone 12.5 mg) tablets, manufactured by Ipca Laboratories Limited, India  1 tablet as a single oral dose of reference product will be administered (as per randomization) with 240 ml of water in sitting posture. 
Comparator Agent  Efnocar 40 (efonidipine HCl ethanolate 40 mg) tablet manufactured by Zuventus Healthcare Limited, India  1 tablet as a single oral dose of reference product will be administered (as per randomization) with 240 ml of water in sitting posture. 
Intervention  Fixed dose combination of Efonidipine HCl ethanolate 40 mg and Chlorthalidone 12.5 mg tablets of Zuventus Healthcare Limited, India  1 tablet as a single oral dose of intervention product will be administered (as per randomization) with 240 ml of water in sitting posture. 
 
Inclusion Criteria  
Age From  18.00 Year(s)
Age To  45.00 Year(s)
Gender  Both 
Details  1. Healthy human subjects between 18-45 years of age (including both) and weight ≥ 50 Kg (BMI 18.5 to 24.9 kg/m² (including both)).
2. Acceptable findings during registration and screening including, medical history, physical examination, laboratory evaluations, 12- lead ECG and Chest X-Ray (postero-anterior view).
3. Values within normal ranges for laboratory parameters upon evaluation by the Investigator or Physician for any of the following tests.
4. Subjects able to communicate effectively.
5. Subjects willing to give written informed consent and adhere to all the requirements of this protocol.
6. Subject willing to abstain from all kinds of alcoholic beverages, grapefruit/caffeine/xanthine containing foods or juices from at least 72.00 hours prior to admission until the last blood sample collection in each study period.
7. Must be non-smokers or ex-smokers: An ex-smoker is defined as someone who completely stopped smoking for at least 6 months before Day 1 (first dosing) of Period-I of this study.
8. Female subjects:
ï‚· Postmenopausal for at least 1 year or
ï‚· Surgically sterile (bilateral tubal ligation, bilateral oophorectomy or hysterectomy has been performed on the subject) or
ï‚· Female of childbearing potential practicing an acceptable method of birth control for the duration of the study as judged by the investigator(s), such as condoms, foams, jellies, diaphragm, and intrauterine device (IUD) or abstinence. 
 
ExclusionCriteria 
Details  1. Subjects having contraindication or hypersensitivity (e.g., anaphylaxis) to Efonidipine and Chlorthalidone or related group of drugs or any of its excipients.
2. A history or presence of significant asthma, urticaria, or other allergic-type reactions after taking aspirin or other NSAIDs. Severe, rarely fatal, anaphylactic-like reactions to NSAIDs, seizures, diabetes, migraine, hypertension, cardiovascular, pulmonary, neurological or psychiatric disease/disorder, dermatological, endocrine, immunological, hepatic, renal, hematopoietic, gastrointestinal, ongoing infectious diseases, or any other significant abnormality as evidenced by medical history and physical examination or according to the opinion of the physician.
3. History or presence of gastrointestinal (GI) inflammation, bleeding, ulceration, and perforation of the stomach, small intestine, or large intestine.
4. History or evidence of exfoliative dermatitis, Stevens - Johnson syndrome (SJS), and toxic epidermal necrolysis (TEN).
5. Any known enzyme inducing or inhibiting drug taken within 30 days before the study.
6. Participation in a drug research study within 90 days prior to dosing of this study.
7. Blood loss or whole blood donation within 90 days prior to drug administration.
8. Consumption of high caffeine (more than 5 cups of coffee or tea/day).
9. History of addiction to any recreational drug or drug dependence.
10. An unusual or abnormal diet, for whatever reason within 48.00 hours prior to admission of each period, e.g. fasting due to religious reasons.
11. History of dehydration from diarrhea, vomiting or any other reason within a period of 72.00 hours prior to study admission of each period.
12. Positive results for drugs of abuse (benzodiazepines, cocaine, opioids, amphetamines, cannabinoids and barbiturates) in urine during the study admission of each period.
13. Positive results for alcohol breathe analysis during the study admission of each period.
14. History of pre-existing bleeding disorder.
15. Difficulty in swallowing solids like tablets.
16. Difficulty with donating blood.
17. Systolic blood pressure less than 120 mm Hg or more than 140 mm Hg.
18. Diastolic blood pressure less than 70 mm Hg or more than 90 mm Hg.
19. Pulse rate less than 60 beats/minute or more than 100 beats/minute.
20. Use of any prescribed medication or OTC medicinal products during last two weeks preceding the first dosing.
21. Female subjects demonstrating a positive pregnancy screen.
22. Female volunteer who is pregnant, currently breast-feeding.
23. Female volunteer who has used implanted or injected hormonal contraceptives anytime during the 6 months prior to study or used hormonal contraceptives within 14 days before dosing. 
 
Method of Generating Random Sequence   Computer generated randomization 
Method of Concealment   Pharmacy-controlled Randomization 
Blinding/Masking   Open Label 
Primary Outcome  
Outcome  TimePoints 
Pharmacokinetic parameters Cmax, AUC0-72hr/AUC0-t and AUC0-inf of Efonidipine and Chlorthalidone from the test product to that of reference product will be assessed.  Pre-dose (00.00 hour) and 00.50, 01.00, 01.25, 01.50, 01.75, 02.00, 02.50, 03.00, 03.50, 04.00, 04.50, 05.00, 06.00, 08.00, 12.00, 24.00, 36.00, 48.00 and 72.00 hours post dose. 
 
Secondary Outcome  
Outcome  TimePoints 
Pharmacokinetic parameters Tmax, T1/2, Kel and AUC_%Extrap_ of Efonidipine and
Chlorthalidone from test products to that of reference product will be assessed. 
Pre-dose (00.00 hour) and 00.50, 01.00, 01.25, 01.50, 01.75, 02.00, 02.50, 03.00, 03.50, 04.00, 04.50, 05.00, 06.00, 08.00, 12.00, 24.00, 36.00, 48.00 and 72.00 hours post dose. 
Assessment of safety and tolerability of single oral dose of test and reference product.  Through-out the study period 
 
Target Sample Size   Total Sample Size="28"
Sample Size from India="28" 
Final Enrollment numbers achieved (Total)= "28"
Final Enrollment numbers achieved (India)="28" 
Phase of Trial   N/A 
Date of First Enrollment (India)   26/03/2021 
Date of Study Completion (India) 24/04/2021 
Date of First Enrollment (Global)  Date Missing 
Date of Study Completion (Global) Date Missing 
Estimated Duration of Trial   Years="1"
Months="0"
Days="0" 
Recruitment Status of Trial (Global)
Modification(s)  
Not Applicable 
Recruitment Status of Trial (India)  Completed 
Publication Details   NIL 
Individual Participant Data (IPD) Sharing Statement

Will individual participant data (IPD) be shared publicly (including data dictionaries)?  

Response - NO
Brief Summary  
STUDY OBJECTIVES
-Primary Objective
To compare the rate and extent of absorption of test product, fixed-dose combination of Efonidipine HCl ethanolate 40 mg and Chlorthalidone 12.5 mg tablets of Zuventus Healthcare Limited, India with Reference products, Efnocar 40 (efonidipine HCl ethanolate 40 mg) tablets, manufactured by Zuventus Healthcare Limited, India and CTD®-12.5 (Chlorthalidone 12.5 mg) tablets, manufactured by Ipca Laboratories Limited, India in normal, healthy, adult, human subjects under fasting conditions.
-Secondary Objective
To assess the safety and tolerability of single oral dose of Efonidipine HCl ethanolate 40 mg and Chlorthalidone 12.5 mg tablets in normal, healthy, adult, human subjects under fasting conditions.
 
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