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CTRI Number  CTRI/2012/05/002703 [Registered on: 28/05/2012] Trial Registered Prospectively
Last Modified On: 05/03/2020
Post Graduate Thesis  No 
Type of Trial  BA/BE 
Type of Study    
Study Design  Randomized, Parallel Group Trial 
Public Title of Study   Bioequivalence study of Fingolimod 0.5 mg capsules  
Scientific Title of Study   An open-label, randomized, single-dose, two-treatment, single period, parallel- group bioequivalence study of Fingolimod 0.5 mg capsules manufactured by MSN Laboratories Limited, India (Test) with GILENYAâ„¢ capsules containing fingolimod 0.5 mg manufactured by Novartis Pharmaceuticals Corporation, U.S.A. (Reference), in healthy, adult, male, human subjects under fasting condition. 
Trial Acronym   
Secondary IDs if Any  
Secondary ID  Identifier 
LBS-PRO-008-11, Version 02, dated 12-Apr-12  Protocol Number 
 
Details of Principal Investigator or overall Trial Coordinator (multi-center study)  
Name  Dr Mukund Zarapkar 
Designation  Vice President - Clinical Research 
Affiliation  LifeSan Clinical Research, division of Centaur Pharmaceuticals Pvt. Ltd. 
Address  Centaur House, Near Hotel Grand Hyatt, Vakola, Santacruz [East], Mumbai 400055

Mumbai
MAHARASHTRA
400055
India 
Phone  912266499154  
Fax  912266499239  
Email  drzarapkar@lifesan.in  
 
Details of Contact Person
Scientific Query
 
Name  Dr Mukund Zarapkar 
Designation  Vice President - Clinical Research 
Affiliation  LifeSan Clinical Research, division of Centaur Pharmaceuticals Pvt. Ltd. 
Address  Centaur House, Near Hotel Grand Hyatt, Vakola, Santacruz [East], Mumbai 400055


MAHARASHTRA
400055
India 
Phone  912266499154  
Fax  912266499239  
Email  drzarapkar@lifesan.in  
 
Details of Contact Person
Public Query
 
Name  Dr Mukund Zarapkar 
Designation  Vice President - Clinical Research 
Affiliation  LifeSan Clinical Research, division of Centaur Pharmaceuticals Pvt. Ltd. 
Address  Centaur House, Near Hotel Grand Hyatt, Vakola, Santacruz [East], Mumbai 400055


MAHARASHTRA
400055
India 
Phone  912266499154  
Fax  912266499239  
Email  drzarapkar@lifesan.in  
 
Source of Monetary or Material Support  
MSN Laboratories Ltd., Hyderabad, India 
 
Primary Sponsor  
Name  MSN Laboratories Ltd 
Address  MSN Laboratories Limited, MSN House, Plot # C 24, Industrial Estate, Sanath Nagar, Hyderabad - 500 018, India Phone: +91- 40-3043 8700 Fax:+91-40-3043 8719 
Type of Sponsor  Pharmaceutical industry-Indian 
 
Details of Secondary Sponsor  
Name  Address 
NIL  NIL 
 
Countries of Recruitment     India  
Sites of Study  
No of Sites = 1  
Name of Principal Investigator  Name of Site  Site Address  Phone/Fax/Email 
Dr Mukund Zarapkar  LifeSan Clinical Research  LifeSan Clinical Research, Centaur House, Near Hotel Grand Hyatt, Vakola, Santacruz [East], Mumbai, 400055
Mumbai
MAHARASHTRA 
912266499154
912266499239
drzarapkar@lifesan.in 
 
Details of Ethics Committee  
No of Ethics Committees= 1  
Name of Committee  Approval Status 
Alert EC-IEC  Approved 
 
Regulatory Clearance Status from DCGI  
Status 
Approved/Obtained 
 
Health Condition / Problems Studied  
Health Type  Condition 
Healthy Human Volunteers  Fingolimod would be used in the treatment of multiple sclerosis 
 
Intervention / Comparator Agent  
Type  Name  Details 
Intervention  Fingolimod 0.5 mg capsules manufactured by MSN Laboratories Limited, India  Single dose of three capsules of 0.5 mg fingolimod will be administered along with 240 mL water after volunteers have fasted for 11 hours prior to dosing 
Comparator Agent  GILENYAâ„¢ capsules containing fingolimod 0.5 mg manufactured by Novartis Pharmaceuticals Corporation, U.S.A.  Single dose of three capsules of GILENYAâ„¢ capsules containing fingolimod 0.5 mgwill be administered along with 240 mL water after volunteers have fasted for 11 hours prior to dosing 
 
Inclusion Criteria  
Age From  18.00 Year(s)
Age To  45.00 Year(s)
Gender  Male 
Details  (i) Healthy, Indian, male, human volunteer aged from 18 to 45 years.
(ii) Body Mass Index (BMI) should be within 18.0-25.0 kg/m2.
(iii) Voluntarily willing and capable to give written and signed informed consent prior to participation in the study, according to the form attached in appendix 1.
(iv) Availability for the entire study period and willingness to adhere to protocol and study requirements.
(v) Volunteers willing to undergo pre and post-study physical examinations and laboratory investigations.
(vi) Having no significant disease or abnormal laboratory values on laboratory examination with no clinical relevance, medical history or physical examination during screening.
(vii) 12-lead ECG in resting position and vital signs are within normal limits or showing or abnormalities, which the investigator does not consider of clinical relevance.
(viii) Normal chest X-Ray findings or findings that have no clinical correlation.
(ix) Non-smokers [Non-smokers at least 6 months from the day of screening].
(x) Having not consumed alcohol at least 48 hours prior to the study justified by negative breath-alcohol test and who agree not to consume any amount of alcohol throughout the conduct of the study.
(xi) Negative urine test for drug of abuse (Amphetamine, barbiturate, tetrahydrocanabinoids, morphine, cocaine and benzodiazepine).
 
 
ExclusionCriteria 
Details  (i) History of allergy or sensitivity to fingolimod or history of any drug hypersensitivity or intolerance, which, in the opinion of the investigator, would compromise the safety of the volunteer if included in the study.
(ii) History of diseases of liver or hepatic impairment within last one (1) year.
(iii) Elevated serum transaminases (SGOT/ SGPT) or alkaline phosphatase. (at least 1.5 times of upper normal range).
(iv) History of mania or hypomania
(v) Serious, uncontrolled disease (including serious psychological disorders) likely to interfere with the study and/or likely to cause death within the study duration.
(vi) Participation in another clinical study or a blood donation program or have had blood loss of more than 350 ml.
(vii) Renal failure (Serum creatinine more than twofold of the > 3 mg/dL).
(viii) Positive blood test for HIV, HbsAg, HCV or VDRL.
(ix) Any clinically relevant abnormality (to be determined by the investigator) following review of screening laboratory data and full physical examination.
(x) Vital sign abnormalities (systolic blood pressure in supine position lower than 100 or higher than 140 mm Hg or diastolic blood pressure lower than 60 or higher than 100 mm Hg or heart rate less than 55 bpm or more than 120 bpm) at screening and at pre-admission physical examination.
(xi) Evidence of second degree or higher AV block, sick sinus syndrome, prolonged QT interval, ischemic cardiac disease, or congestive heart failure in the 12-lead ECG is resting supine position.
(xii) Having suffered any illness within a week of starting the study or who have been hospitalized within the last 3 months preceding the start of the study.
(xiii) Having taken over the counter (OTC) or prescribed medications, including any antihypertensive drugs, enzyme-modifying drugs or any systemic medication within the 07 days prior to the study (However, paracetamol may be permitted up to 03 days prior to the start of the study).
(xiv) Have a history of substance abuse within the last 5 years.
(xv) Consumption of xanthine-containing derivatives (coffee, tea, cola drinks, chocolate) within 48 hrs before IMP administration and grapefruit or orange juice for at least 48 hrs prior to IMP administration.
(xvi) Habit of chewing or inhaling nicotine-containing products (e.g. tobacco) currently or within last six months prior to the study.
(xvii) Abnormal INR combined with clinical manifestation
 
 
Method of Generating Random Sequence   Stratified block randomization 
Method of Concealment   Pharmacy-controlled Randomization 
Blinding/Masking   Open Label 
Primary Outcome  
Outcome  TimePoints 
To evaluate the bioequivalence between two formulations i.e. Fingolimod 0.5 mg capsules manufactured by MSN Laboratories Limited, India (Test) and GILENYAâ„¢ capsules containing fingolimod 0.5 mg manufactured by Novartis Pharmaceuticals Corporation, U.S.A. (Reference), following single dose of three capsules of test or reference formulation in healthy, adult, male, human subjects under fasting condition by means of rate and extent of absorption.  0.00 (predose), 1.00, 2.00, 4.00, 6.00, 8.00, 10.00, 12.00, 14.00, 16.00, 24.00, 36.00, 48.00, 72.00, 96.00, 144.00, 192.00, 240.00, 288.00, 336.00, and 432.00 hours after IMP administration 
 
Secondary Outcome  
Outcome  TimePoints 
To monitor the safety of the participating subjects in this bioequivalence study of Fingolimod 0.5 mg capsules manufactured by MSN Laboratories Limited, India (Test) and GILENYAâ„¢ capsules containing fingolimod 0.5 mg manufactured by Novartis Pharmaceuticals Corporation, U.S.A. (Reference) formulation, determined by means of clinical biochemistry, physical examination and AE/SAE monitoring  throughout the study 
 
Target Sample Size   Total Sample Size="32"
Sample Size from India="32" 
Final Enrollment numbers achieved (Total)= "0"
Final Enrollment numbers achieved (India)="32" 
Phase of Trial   N/A 
Date of First Enrollment (India)   11/06/2012 
Date of Study Completion (India) 02/07/2012 
Date of First Enrollment (Global)  Date Missing 
Date of Study Completion (Global) Date Missing 
Estimated Duration of Trial   Years="0"
Months="6"
Days="0" 
Recruitment Status of Trial (Global)
Modification(s)  
Not Applicable 
Recruitment Status of Trial (India)  Completed 
Publication Details   Not yet decided 
Individual Participant Data (IPD) Sharing Statement

Will individual participant data (IPD) be shared publicly (including data dictionaries)?  

Brief Summary  
Single-dose, randomized, open-label, two-treatment, single period, parallel-group bioequivalence study in the healthy, adult, male, human subjects under fasting condition is employed to evaluate the bioequivalence between two formulations i.e. Fingolimod 0.5 mg capsules manufactured by MSN Laboratories Limited, India (Test) and GILENYAâ„¢ capsules containing fingolimod 0.5 mg manufactured by Novartis Pharmaceuticals Corporation, U.S.A. (Reference), following single dose of three capsules of test or reference formulation in healthy, adult, male, human subjects under fasting condition by means of rate and extent of absorption and monitor the safety of the participants. Blood will be collected at twenty-one (21) timepoints, (5 mL each) in K3EDTA vacutainers in this single period study at following time points: 0.00 (predose), 1.00, 2.00, 4.00, 6.00, 8.00, 10.00, 12.00, 14.00, 16.00, 24.00, 36.00, 48.00, 72.00, 96.00, 144.00, 192.00, 240.00, 288.00, 336.00 and 432.00 hours after IMP administration. All the collected blood samples will be divided into two suitably pre-labelled transparent polypropylene cryovials and stored immediately at -80° ± 10°C in the bioanalytical department. Each tube or cryovial will have at least 2.5 mL of blood. After completion of the study, segregation of aliquots will be done in the bioanalytical department under controlled temperature conditions [using dry ice etc.] to avoid thawing. Samples from all evaluable subjects who complete the study will be assayed for only fingolimod. All the samples will be transferred from the clinical to the analytical facility after completion of the study. The analytical unit is responsible for accurate and precise quantification of fingolimod during analysis, quality control samples will be distributed throughout each batch of study samples. The analyst will not have access to the randomisation schedule.
 
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