Central venous access not only plays an important role in the management of critically ill patients and but also puts the patients at risk of iatrogenic complications. Central Venous Catheters (CVCs) are indispensible in current medical and intensive care treatment and are commonly associated with hospital acquired bloodstream infections. It increases the risk of Catheter Related Infection (CRI) resulting in increased morbidity and mortality. The rate of CVC-BSI across Indian hospitals range from 4.01/1000 catheter days to 9.26/1000 catheter days denoting the need for effective infection control programmes including surveillance and antibiotic policies. Gram-positive cocci - Staph aureus, Staph epidermidis, Staph saprophyticus and MRSA are responsible for at least two-thirds of the infections followed by Gram-negative bacilli - Klebsiella pneumonia, E. Coli, Pseudomonas aeruginosa, Enterococcus spp and Acinetobacter spp. The preventive strategies to reduce the rate of CVC-BSI include the use of antimicrobial impregnated catheters and also aseptic catheter insertion techniques. Due to the scarcity of CVC-BSI data from our region, this study will be taken up in our ICU of the tertiary care hospital to determine the microbiological profile and antimicrobial susceptibility of the CVC-BSI isolates.