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CTRI Number  CTRI/2019/12/022266 [Registered on: 05/12/2019] Trial Registered Prospectively
Last Modified On: 07/03/2020
Post Graduate Thesis  No 
Type of Trial  Interventional 
Type of Study   Drug 
Study Design  Other 
Public Title of Study   A Study of PNB-001 (Test, Manufactured by PNB Vesper Life Science Pvt. Ltd., India). 
Scientific Title of Study   A Placebo-controlled, Randomized, Double Blind, Multiple Ascending Dose, Multiple Cohort, Multicentre Study to Assess Safety, Tolerability, Pharmacokinetics & Efficacy of PNB-001 (Test, Manufactured by PNB Vesper Life Science Pvt. Ltd., India). 
Trial Acronym   
Secondary IDs if Any  
Secondary ID  Identifier 
0218-19, Version no. 2.0, Date: 04 July 2019  Protocol Number 
 
Details of Principal Investigator or overall Trial Coordinator (multi-center study)  
Name  Dr Anshul Attrey 
Designation  Assistant General Manager 
Affiliation  Lambda Therapeutic Research Ltd 
Address  Lambda House, Department of Clinical Pharmacology and Medical Affairs, Plot No. 38, Survey No. 388
Near Silver Oak Club, S. G. Highway, Gota
Ahmadabad
GUJARAT
382481
India 
Phone  07940202274  
Fax  07940202021  
Email  anshulattrey@lambda-cro.com  
 
Details of Contact Person
Scientific Query
 
Name  Dr Anshul Attrey 
Designation  Assistant General Manager 
Affiliation  Lambda Therapeutic Research Ltd 
Address  Lambda House, Department of Clinical Pharmacology and Medical Affairs, Plot No. 38, Survey No. 388
Near Silver Oak Club, S. G. Highway, Gota

GUJARAT
382481
India 
Phone  07940202274  
Fax  07940202021  
Email  anshulattrey@lambda-cro.com  
 
Details of Contact Person
Public Query
 
Name  Mr Prashant Modi 
Designation  Senior General Manager 
Affiliation  Lambda Therapeutic Research Ltd 
Address  Lambda House, Department of Project Management & Regulatory Affairs, Plot No. 38, Survey No. 388
Near Silver Oak Club, S. G. Highway, Gota
Ahmadabad
GUJARAT
382481
India 
Phone  07940202375  
Fax  07940202021  
Email  prashantmodi@lambda-cro.com  
 
Source of Monetary or Material Support  
PNB Vesper Life Science Pvt. Ltd, Door No. 40/1045G, 5th Floor, Amrutha Towers, Cochin, Kerala 682011, India, Tel. No.: 09446331136 
 
Primary Sponsor  
Name  PNB Vesper Life Science Pvt Ltd 
Address  Door No. 40/1045G, 5th Floor, Amrutha Towers, Cochin, Kerala 682011, India, Tel. No.: 09446331136 
Type of Sponsor  Pharmaceutical industry-Indian 
 
Details of Secondary Sponsor  
Name  Address 
NA  NA 
 
Countries of Recruitment     India  
Sites of Study
Modification(s)  
No of Sites = 7  
Name of Principal Investigator  Name of Site  Site Address  Phone/Fax/Email 
Dr Sandip P Shah  Gastroplus Digestive Disease Centre  Address of the Principal Investigator,Department of clinical research D Block, 3rd Floor, Galaxy Bazar, Sunrise Park Road, Vastrapur,
Ahmadabad
GUJARAT 
09909933006

drsandirnrnstror@gmail.com 
Dr Ashish Kumar Shah  GMERS Medical College and Hospital  Department of clinical research,Room No.GotriOld TB Hospital Campus Gotri Main Road, Gotri- 390021
Vadodara
GUJARAT 
08849450012

drashishshah2001@yahoo.com 
Dr Keyur Brahme Madan  Government Medical College & Shri Sayajirao General Hospital,   Department of clinical research,Room No. NA,Anandpura-390001
Vadodara
GUJARAT 
09727729105

keyurbrahme@gmail.com 
Dr Sanjiv Haribhakti  Kaizen Hospital, Institute of Gastroenterology & Research Centre  Department of clinical research,A Unit of G.I. Healthcare Pvt. Ltd., 132 Ft. Ring road, Helmet Circle, Memnagar-380052
Ahmadabad
GUJARAT 
09824047457

sharibhakti@gmail.com 
Dr Anshul Attrey  Lambda Therapeutic Research Ltd  Lambda House, Department of Clinical Pharmacology and Medical Affairs, Plot No.38 survey no. 388, Near Silver oak club, S.G. Highway Gota -382481
Ahmadabad
GUJARAT 
07940202274

anshulattrey@lambda-cro.com 
Dr B V Hemavathi  Mythri Hospital Mehdipatnam  Department of clinical research,Opp. Pilar No.81, PVNR Expressway, Mehdipatnam, Ring Road-500008
Hyderabad
TELANGANA 
09866671418

ccr.drbvhemavathi@gmail.com 
Dr Bhumit Patel  Shrey Hospital Pvt. Ltd.  Department of clinical research,Room No:270/5/B,Near AMCO Bank, Stadium Circle, Navarangpura-380009
Ahmadabad
GUJARAT 
08412883230

bhumitpatel82@gmail.com 
 
Details of Ethics Committee
Modification(s)  
No of Ethics Committees= 7  
Name of Committee  Approval Status 
Ethics Committee, Mythri Hospital Mehdipatnam,Dr B V Hemavathi  Approved 
Gastro Plus Ethics Committee,,Dr Sandip P Shah  Approved 
Institutional Ethics Committee for Human Research,Dr. Keyur Brahme Madan  Submittted/Under Review 
Institutional Human Ethics Committee,GMERS Medical College and Hospital,Dr. Ashish Kumar Shah  Approved 
Kaizen Ethics Committee,Dr Sanjiv Haribhakti   Submittted/Under Review 
Riddhi Medical Nursing home Institutional ethics committee, Dr Anshul Attrey  Approved 
Shrey Hospital Institutional Ethics Committee,Dr. Bhumit Patel  Submittted/Under Review 
 
Regulatory Clearance Status from DCGI  
Status 
Approved/Obtained 
 
Health Condition / Problems Studied  
Health Type  Condition 
Healthy Human Volunteers  Healthy Male & healthy surgically sterilize female subjects 
Patients  (1) ICD-10 Condition: N946||Dysmenorrhea, unspecified, (2) ICD-10 Condition: K519||Ulcerative colitis, unspecified,  
 
Intervention / Comparator Agent  
Type  Name  Details 
Comparator Agent  Placebo treatment of PNB Vesper Life Science Pvt. Ltd., India.  Identical formulation without active ingredient 
Intervention  PNB-001 of PNB Vesper Life Science Pvt. Ltd., India  Dose, Frequency and Duration of treatment: In healthy (BID for 14 days): 50 mg, 100 mg, 200 mg; In patients: IBD – 50 mg BID for 28 days Dysmenorrhea – 50 mg TID for 3 days; Mode of Administration: Per oral  
 
Inclusion Criteria  
Age From  18.00 Year(s)
Age To  45.00 Year(s)
Gender  Both 
Details  For Healthy subjects:
a) Non-smokers, Healthy, adult, human volunteers between 18 and 45 years of age (both inclusive).
b) Having a Body Mass Index (BMI) between 18.5 to 29.9 (both inclusive), calculated as weight in kg/height in m2.
c) Have no significant diseases or clinically significant abnormal findings during screening, medical history, clinical examination, laboratory evaluations, 12-lead ECG and X-ray chest (postero-anterior view) recordings.
d) Able to understand and comply with the study procedures, in the opinion of the investigator.
e) Able to give voluntary informed consent for participation in the trial.
f) In case of female subjects:
i. Surgically sterilized at least 6 months prior to study participation;
And
ii. Serum pregnancy test must be negative

For IBD Patients:
a) Male patients are eligible, if they are ≥ 18 years and ≤ 65 years of age.
b) Patients have a documented history of idiopathic ulcerative colitis based on standard endoscopic (colonoscopic) and histological criteria involving the whole/part of left side of the colon [approximately 60 cm up from the anus (anal verge) to splenic flexure of colon], with mild to moderate active disease.
c) Patients having ESR, CRP and FC levels more than upper limit of normal.
d) Patients with a Full Ulcerative Colitis Disease Activity Index (UCDAI) score of 4-10.
e) Rectal bleeding score (based on subject diary) of 1 or more at baseline.
f) Mucosal appearance score (based on endoscopy) of 1 point or more at baseline.
g) Stool Frequency subscore of 1 or more at baseline.
h) Patients who have failed to achieve total resolution of all symptoms to topical and/or oral standard treatment regimen of mesalamine over minimum 4 week duration and are in need of an alternative therapy as per the discretion of the Investigator.
i) Male subjects must be surgically sterile, abstinent, or patient or partner compliant with a contraceptive regimen from screening until 4 weeks after the last dose of study medication..
j) They are able to understand and sign a written informed consent form, which must be obtained prior to initiation of study procedures.
k) Subjects with acceptable laboratory parameters.
For dysmenorrhoea (surgically sterilized females) Patients:
a) Females with regular cycles (21–35 days) with duration of 3 to 7 days.
b) Females with primary dysmenorrhea.
c) Females who are surgically sterile.
d) Patients with moderate to severe pain on visual analogue scale (VAS ≥45 mm) in the current and previous 3 menstrual cycles, necessitating the use of analgesics.
e) Pain lasting for at least 2 days.
f) Willing to provide written informed consent. 
 
ExclusionCriteria 
Details  For Healthy subjects:
a. Known hypersensitivity or idiosyncratic reaction to cholecystokinin receptor antagonist or to any of its excipients or any drug or any substance.
b. History or presence of any disease or condition which might compromise the haemopoietic, renal, hepatic, endocrine, pulmonary, central nervous, cardiovascular, immunological, dermatological, gastrointestinal or any other body system.
c. Sitting blood pressure less than 110/70 mm Hg and/or more than 140/90 mm Hg at the time of screening.
d. Ingestion of a medicine [prescribed & over the counter (OTC) medication including herbal remedies] at any time within 2 weeks before first dosing (4 weeks for any drug which may affect hepatic drug metabolism). In any such case subject selection will be at the discretion of the Principal Investigator.
e. Any history or presence of asthma (including aspirin induced asthma) or nasal polyp or NSAIDs induced urticaria.
f. Consumption of Grapefruits or its products within a period of 72 hours prior to first IMP administration.
g. A recent history of harmful use of alcohol (less than 2 years), i.e. alcohol consumption of more than 14 standard drinks per week for men and more than 7 standard drinks per week for women (A standard drink is defined as 360 ml of beer or 150 ml of wine or 45 ml of 40% distilled spirits, such as rum, whisky, brandy etc) or Consumption of alcohol or alcoholic products within 48 hours prior to first IMP administration.
h. Smokers or who have smoked within last 06 months prior to start of the study.
i. Difficulty in swallowing solids dosage forms like capsule or tablets.
j. The presence of clinically significant abnormal laboratory values during screening.
k. Use of any recreational drugs or history of drug addiction or testing positive in pre-study drug scans.
l. A history of difficulty with donating blood.
m. Donation of blood (1 unit or 350 mL) within a period of 90 days prior to the first dose of study medication.
n. Receipt of an investigational medicinal product or participation in a drug research study within a period of 90 days prior to the first dose of study medication.
If investigational medicinal product is received within 90 days where there is no blood loss except safety lab testing, subject can be included considering 10 half-lives duration of investigational medicinal product received.
o. A positive hepatitis screen including hepatitis B surface antigen and/or HCV antibodies.
p. A positive test result for HIV antibody (I &/or II).
q. An unusual diet, for whatever reason (e.g. low-sodium), for four weeks prior to first IMP administration. In any such case subject selection will be at the discretion of the Principal Investigator.
r. Nursing mothers (females)
s. Females of child bearing potential.
For IBD Patients:
a. Documented history of proximal or universal ulcerative colitis (pan colitis).
b. Those who receive a Physician rating of disease severity of 3 as part of the modified UCDAI or Full UCDAI aggregate score of 11 or greater (severe disease).
c. Patients with known allergy to study drugs or have a history of serious AEs related to their use.
d. Patients who demonstrate signs and symptoms of fulminant colitis, bowel stricture, toxic megacolon, an anticipated need for blood transfusion for gastrointestinal bleeding, or demonstrate evidence of peritonitis.
e. Prior documented history of evidence of high grade dysplasia on biopsy from endoscopic examinations.
f. Patient whose stool contains enteric pathogens or Clostridium difficile toxins.
g. History of recurrent Clostridium difficile infection.
h. History of biological therapy with agents like infliximab, adalimumab, etanercept, etc. within the previous 8 weeks of screening and patients in need of such therapy during the study period.
i. Patients who received systemic steroids or immunosuppressants within the previous 4 weeks of screening.
j. Treatment in the last 1 week of screening that included antibiotic, antifungal, or antiparasitic medications.
k. Patients having a history of cancer (defined as malignancy), asthma, or bronchospasm.
l. Patients not able to withdraw and in need of continuing other immunosuppressants like sirolimus or cyclosporine during the study period.
m. There is evidence of chemical substance abuse.
n. Patients with Crohn disease.
o. Patients with HIV, Hepatitis B and Hepatitis C infection.
p. Patients with pre existent renal function disorders, liver function disorders, cardiac disease, hypertension, clinically important hematological, metabolic, psychiatric, central nervous system or pulmonary disease.
q. Patients who participated in any other clinical or post-marketing study (not only for study drugs but also for medical devices) 30 days before signing the informed consent.
r. Any condition which the study physician judges to preclude safe participation in the study or to confound the evaluation of the study outcome.
For Dysmenorrhoea (surgically sterilized females) Patients:
a. Hypersensitivity to the study medications.
b. Active or suspected gastrointestinal ulcer, chronic dyspepsia, or gastrointestinal bleeding; Crohn’s disease or ulcerative colitis; history of significant chronic constipation or recurrent colitis.
c. History of bronchial asthma.
d. Severe heart failure.
e. Moderate-to-severe renal function (GFR < 60 mL/min).
f. Severely impaired hepatic function.
g. Hemorrhagic diathesis and other coagulation disorders.
h. Contraindication to use of PNB-001.
i. Diagnosed gastrointestinal obstruction; myasthenia gravis; glaucoma.
j. Planning to get married during the study period.
k. Known or suspected secondary dysmenorrheal.
l. Other causes of chronic pelvic pain (previous major abdominal or pelvic surgery, endometriosis, pelvic inflammatory disease, ovarian cysts, pathological vaginal secretion, chronic abdominal pain, inflammatory bowel disease, irritable bowel syndrome).
m. Hormonal therapy during the last 6 months or planning to take hormonal therapy during the study period.
n. Regular intake of pain medications (including NSAIDs) for other reason than dysmenorrhea, digoxin, antidepressants, tranquilizers, hypnotics, sedatives, or sex hormones; any concomitant disease or condition that might require any intake of analgesic medication; evidence of clinically relevant serious condition whether gynecological, hematological, pulmonary endocrinological, autoimmune diseases, neurologic or psychiatric. 
 
Method of Generating Random Sequence   Computer generated randomization 
Method of Concealment   Not Applicable 
Blinding/Masking   Participant and Investigator Blinded 
Primary Outcome  
Outcome  TimePoints 
Assessment of the safety and tolerability of study drug.  Through out the study 
 
Secondary Outcome  
Outcome  TimePoints 
Assessment of pharmacokinetics and efficacy of study drug.  Pre-dose
Day 01, Day 12, Day 13 and Day 14
Post dose
Day 01 (0.500, 1.000, 1.500, 2.000, 2.500, 3.000, 3.500, 4.000, 4.333, 4.667, 5.000, 5.333, 5.667, 6.000, 6.333, 6.667, 7.000, 7.500, 8.000, 9.000, 10.000 and 12.000 hours)
Day 14 (0.500, 1.000, 1.500, 2.000, 2.500, 3.000, 3.500, 4.000, 4.333, 4.667, 5.000, 5.333, 5.667, 6.000, 6.333, 6.667, 7.000, 7.500, 8.000, 9.000, 10.000, 12.000, 16.000, 20.000 and 24.000 hours) 
 
Target Sample Size   Total Sample Size="52"
Sample Size from India="52" 
Final Enrollment numbers achieved (Total)= "Applicable only for Completed/Terminated trials"
Final Enrollment numbers achieved (India)="Applicable only for Completed/Terminated trials" 
Phase of Trial   Phase 1 
Date of First Enrollment (India)   08/12/2019 
Date of Study Completion (India) Applicable only for Completed/Terminated trials 
Date of First Enrollment (Global)  Date Missing 
Date of Study Completion (Global) Applicable only for Completed/Terminated trials 
Estimated Duration of Trial   Years="0"
Months="5"
Days="0" 
Recruitment Status of Trial (Global)
Modification(s)  
Not Applicable 
Recruitment Status of Trial (India)  Open to Recruitment 
Publication Details   None Yet 
Individual Participant Data (IPD) Sharing Statement

Will individual participant data (IPD) be shared publicly (including data dictionaries)?  

Brief Summary  
The Sponsor has developed the test product. This study is being conducted to assess the safety, tolerability, pharmacokinetics & efficacy of PNB-001 after multiple ascending dose of PNB-001.The current study is a placebo-controlled, randomized, double blind, multiple ascending dose, multicentre study. The study will be conducted in multiple cohorts. It is a descriptive study to evaluate multiple-dose safety and tolerability and to estimate dose-limiting toxicity, if any, of multiple oral doses. Doses selected for the study range from 50 mg to 200 mg BID. Animal models suggest the anti-inflammatory and analgesic potential of PNB-001. To evaluate these properties of PNB-001 in patient population the current study will involve two patient based cohorts one each of ulcerative colitis and dysmenorrhea wherein the efficacy of the molecule will be evaluated. A total of 52 subjects will be enrolled into the study. The study will commence only after a written approval is obtained from the Institutional Review Board and written informed consent given by the subjects.
 
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