Tuberculosis (TB) is one of the commonest communicable diseases and a leading cause of mortality and morbidity worldwide, especially in developing countries. World Health Organization (WHO) in 2016 considered TB as the top infectious killer which caused 1.8 million deaths in the year 2015. As per Global TB Report 2016, India has 2.8 million new cases of TB and 1,30,000 cases of MDR TB annually. It has also highest TB-HIV co-morbidity in the world and an estimated 1.1 lakh HIV associated TB occurred in 2015 and 37,000 estimated number of patients died among them. In order to control TB, a highly specific and sensitive rapid diagnostic test is prerequisite for early and effective treatment of TB patients there by breaking the transmission chain. In December 2010, WHO recommended use of CB-NAAT for detection of TB and  rifampicin resistance in clinical specimens.  In order to know the isoniazid sensitivity additionally, the smear positive sputum specimens were subjected Line Probe Assays (LPAs). Hence combining the two tests the turnaround time takes about 5-6 days usually in programmatic condition. However, while the simplicity of the design (from the operator perspective) is a key advantage in many settings, it is less crucial in relatively well-equipped centralized laboratories. Instrument/assay combinations that are designed for this level of the healthcare system should in principle is able to achieve similar performance with higher throughput and at a lower per-sample cost – a key advantage in the resource-limited settings where TB is most common and where highly decentralised testing is economically unfeasible. Several novel assays have been recently developed/CE-marked that are all aimed at use in centralized laboratories with potential for high sample throughput and high accuracy for detection of MTB as well as RIF- and INH-resistance. However, published data on their performance and operational characteristics is extremely limited. This study may help immensely to the TB elimination programme of India as high through put and  less TAT are the predominant characteristic of the intended study