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CTRI Number  CTRI/2011/10/002076 [Registered on: 19/10/2011] Trial Registered Prospectively
Last Modified On: 25/04/2012
Post Graduate Thesis  No 
Type of Trial  Interventional 
Type of Study   Drug 
Study Design  Randomized, Parallel Group, Active Controlled Trial 
Public Title of Study   A clinical study to estimate the safety, tolerability and efficacy of two medications for treatment of complicated intra abdominal infections in adults. 
Scientific Title of Study   A Phase 2, Randomized, Double-Blind, Double-Dummy, Multicenter, Prospective Study to Assess the Efficacy, Safety and Pharmacokinetics of 2 Dose Regimens of TP-434 Compared with Ertapenem in Adult Community- Acquired Complicated Intra-abdominal Infections 
Trial Acronym   
Secondary IDs if Any  
Secondary ID  Identifier 
NCT01265784  ClinicalTrials.gov 
2010-022548-19  EudraCT 
TP-434-P2-cIAI-1  Protocol Number 
 
Details of Principal Investigator or overall Trial Coordinator (multi-center study)  
Name  Sunil Garg 
Designation  Senior Manager 
Affiliation   
Address  INC GVK Bio Pvt Ltd. 14th Floor, Tower B, Building No 14 DLF Cyber City, Phase III, Gurgaon 122002, Haryana (INDIA)

Gurgaon
HARYANA
122002
India 
Phone  0124-4642469  
Fax  0124-4642401  
Email  sgarg@incresearch.com  
 
Details of Contact Person
Scientific Query
 
Name  Dr Mamta Singh 
Designation  Senior Manager 
Affiliation   
Address  INC GVK Bio Pvt Ltd. 14th Floor, Tower B, Building No 14 DLF Cyber City, Phase III, Gurgaon 122002, Haryana (INDIA)

Gurgaon
HARYANA
122002
India 
Phone  0124-4642449  
Fax  0124-4642401  
Email  masingh@incresearch.com  
 
Details of Contact Person
Public Query
 
Name  Dr Mamta Singh 
Designation  Senior Manager 
Affiliation   
Address  INC GVK Bio Pvt Ltd. 14th Floor, Tower B, Building No 14 DLF Cyber City, Phase III, Gurgaon 122002, Haryana (INDIA)

Gurgaon
HARYANA
122002
India 
Phone  0124-4642449  
Fax  0124-4642401  
Email  masingh@incresearch.com  
 
Source of Monetary or Material Support  
Tetraphase Pharmaceuticals Inc, 480 Arsenal Street, Suite 110 Watertown, MA 02472, USA 
 
Primary Sponsor  
Name  Tetraphase Pharmaceuticals Inc 
Address  480 Arsenal Street, Suite 110 Watertown, MA 02472, USA 
Type of Sponsor  Pharmaceutical industry-Global 
 
Details of Secondary Sponsor  
Name  Address 
NIL  NIL 
 
Countries of Recruitment     India
Latvia
Lithuania
Romania
United States of America  
Sites of Study  
No of Sites = 11  
Name of Principal Investigator  Name of Site  Site Address  Phone/Fax/Email 
Dr Surendran  Amrita Institute of Medical Science   Dept of Gastrointestinal surgery and Vascular surgery, AIMS Post, Ponekkara, Kochi-682041
Kozhikode
KERALA 
0484-2802134
0484-2802134
sudhi@aims.amrita.edu 
Dr Swastik Nandi  BP Poddar Hospital & Medical Research Ltd.  Dept of Surgery, 71/1, Humayun Kabir Sarani, Block-G, New Alipore, Kolkata, 700053
Kolkata
WEST BENGAL 
033-23997009
033-23997009
swastiknandi@yahoo.com 
Hosmath Vijayakumar  Gokula Metropolis Clinical Research Centre  Department of Surgery, MSR Nagar, MSRIT Post, New BEL Road, Bangalore-560054
Bangalore
KARNATAKA 
080-40528402
080-40528402
drvkhosmath@gmail.com 
Dr Hariprasad Taluru  K.R. Hospital   Dept of General Surgery, No. 979, 25th Main Road, BSK, 1st Stage, 50 Feet Road, Bangalore 560050
Bangalore
KARNATAKA 
080-26743596
080-26743596
harishermi@hotmail.com 
Dr Nitin Pai  KEM Hospital  Dept of Gastroenterology, Sardar Moodliar Road, Rasta Peth, Pune-411011
Pune
MAHARASHTRA 
020-26125603
020-26125603
drnitinpai@gmail.com 
Dr Manish Bhatnagar  Medisurge Hospital  Room No. 101, Research and Development Department, Mithakhali Six Roads, Ellisbridge, Ahmedabad-380006
Ahmadabad
GUJARAT 
079-26441401

man_bhatnagar@yahoo.com 
Dr Mukesh Kalla  S.R.Kalla Memorial Gastro. & Gen. Hospital  Dept of gastroenterology,78, Dhuleshwar Garden, Behind HSBC Bank, Sardar Patel Marg, C-Scheme, Jaipur-302001
Jaipur
RAJASTHAN 
0141-4020622
0141-4020622
drmkalla@rediffmail.com 
Dr Suresh Jain  Sahyadri Munot Hospital  Dept of Gastroenterology, 387/5 Bhavani Peth, New Timbermarket Road, Near Seven Loves Chowk, Pune-411042
Pune
MAHARASHTRA 
020-67213800
020-67213800
scjdr@yahoo.com 
Dr P P Sethu Babu  Sai Vani Hospitals Ltd  Department of Gastroenterology, 1-2-365/36/6 & 7, Ramakrishna Math Road, Opp. Indira Park, Domalguda, Hyderabad-500029
Hyderabad
ANDHRA PRADESH 
040-27634462
040-27636882
sethu9@rediffmail.com 
Dr Yogishwara Chowdipallaya Nagappa  Santosh Hospital,  Department of Surgery, No.6/1, Promenade Road, Near Good Will School, Behind Coles Park, Bangalore-560005
Bangalore
KARNATAKA 
9900003299

yogishcn@yahoo.co.in 
Dr M K Ramesh  Victoria Hospital  Room No.140, Ground Floor, Centenary Building, Fort, Bangalore-560002
Bangalore
KARNATAKA 
080-26702386
080-26702386
drmkramesh@gmail.com 
 
Details of Ethics Committee  
No of Ethics Committees= 11  
Name of Committee  Approval Status 
B.P Poddar Hospital & Medical Research Ltd Ethical Committee  Submittted/Under Review 
Bangalore Central Ethics Committee  Approved 
Bangalore Medical College and Research Institute  Submittted/Under Review 
Bangalore, central ethics committee  Approved 
Ethics Committee KEM Hospital Research  Submittted/Under Review 
Ethics Committee, KEM Hospital Research  Submittted/Under Review 
HCG Medi-Surge Ethics Committee  Approved 
M.S. Ramaiah Medical College and Technical Hospital  Approved 
S.R. Kalla Memorial Ethical Committee for Human research  Approved 
Sahyadri Hospital Ltd. Ethics Committee  Submittted/Under Review 
Virtuous Independent Ethics Committee  Approved 
 
Regulatory Clearance Status from DCGI  
Status 
Approved/Obtained 
 
Health Condition / Problems Studied  
Health Type  Condition 
Patients  Acutely hospitalized subjects with a clinical diagnosis of CA-cIAI requiring surgery will be recruited into this study.,  
 
Intervention / Comparator Agent  
Type  Name  Details 
Comparator Agent  Ertapenam 1g  Ertapenem 1g administered by IV q24h. Ertapenem infusion solutions will be prepared according to the full prescribing information of Invanz® (comparator) Duration of Therapy: No longer than 7 days 
Intervention  TP 434 for Intravenous administration  TP-434-046 was synthesized under current good manufacturing practices and the drug product contains 52.5 mg (TP-434 free base equivalents) of lyophilized powder in a 30 mL vial. TP-434 will be reconstituted with 10 mL of sterile water and further diluted with sterile 0.5x normal saline, to generate 0.3 mg/mL TP 434 solution for 1.5mg / Kg q 24h IV infusion. Duration of Therapy: no longer than 7 days 
Intervention  TP-434 for intravenous administration  TP-434-046 was synthesized under current good manufacturing practices and the drug product contains 52.5 mg (TP-434 free base equivalents) of lyophilized powder in a 30 mL vial. TP-434 will be reconstituted with 10 mL of sterile water and further diluted with sterile 0.5x normal saline, to generate 0.2 mg/mL TP-434 solution for 1.0 mg/kg q12 h IV infusion. Duration of Therapy: no longer than 7 days 
 
Inclusion Criteria  
Age From  18.00 Year(s)
Age To  75.00 Year(s)
Gender  Both 
Details  1. Abdominal pain or discomfort with onset prior to hospitalization
2. Evidence of a systemic inflammatory response with at least one of the following
a. Fever (oral temperature greater than 100.4 degree Fahrenheit or 38 degree Celsius) or hypothermia (oral temperature less than 35 degree Celsius/95 degree Fahrenheit).
b. Elevated white blood cell (WBC) count (greater than 10,500 per cubic mm)
c. Tachycardia (Heart rate greater 90 beats per min)
d. Tachypnea (greater than 20 breaths per min)
3. Physical findings consistent with intra-abdominal infection (IAI, defined as localized or diffuse abdominal tenderness (Other supportive findings include presence of a mass, ileus, or rebound tenderness)
4. Clinical diagnosis of community-acquired intra-abdominal infection requiring urgent surgical or percutaneous intervention and not expected to require antibacterial therapy for longer than 14 days
5. At least 18 years and no older than 75 years of age
6. Body mass index (BMI) of less than or equal to 30 kg per meter square
7. Able to provide informed consent. If the patient is unable to provide informed consent, the patient’s legally acceptable representative may provide written consent in accordance with institutional guidelines
If female:
a. Not pregnant or nursing
b. If of child-bearing potential will commit to either:
i. use at least two medically accepted, effective methods of birth control (e.g., condom, oral contraceptive, indwelling intrauterine device, hormonal implant/patch, injections, approved cervical ring) during study drug dosing and for 90 days following last study drug dose
ii. Sexual abstinence
And either
9A. Meets Inclusion Criteria for Pre-operative Enrollment
a) Has a sonogram or radiographic imaging result congruent with the diagnosis of cIAI
b) Acute surgical or percutaneous intervention (open laparotomy, laparoscopic surgery, or percutaneous drainage of an abscess) is foreseen
c) Specimens from the surgical intervention representative of the material associated with infection will be collected by aspiration or tissue sample and sent for culture and susceptibility testing
d) Clinical diagnoses consistent with one or more of the following
1. Appendiceal perforation and periappendiceal abscess
2. Diverticular abscess
3. Acute gastric or duodenal perforation (only if operated on greater than 24 hrs after perforation occurs)
4. Traumatic perforation of the intestines (only if operated on greater than 12 hrs after perforation occurs)
5. Abscess or peritonitis due to other perforated viscus, or other gastrointestinal source
or
9B. Meets Inclusion Criteria for Intra-operative or Post-operative Enrollment
a) Visual confirmation (presence of pus within the abdominal cavity)
b) Samples taken for aerobic and anaerobic cultures should be taken by aspiration or tissue sample and immediately inoculated to appropriate media
c) Surgical intervention includes open laparotomy, laparoscopic surgery, or percutaneous draining of an abscess
d) Initial intervention is adequate, that is a procedure in which all communications between the GI tract and the peritoneal cavity are closed, no necrotic intestine is left, and all infected collections are drained at the initial procedure
e) Intra-operative diagnoses include one or more of the following
1. Appendiceal perforation and periappendiceal abscess
2. Diverticular abscess
3. Acute gastric and duodenal perforations (only if operated on greater than 24 hrs after perforation occurs)
4. Traumatic perforation of the intestines (only if operated on greater than 12 hrs after perforation occurs)
5. Abscess or peritonitis due to perforated viscus, or other focus of infection (but not spontaneous bacterial peritonitis associated with cirrhosis and chronic ascites)
6. Other intra-abdominal abscess (excluding liver and spleen) from a gastrointestinal source
 
 
ExclusionCriteria 
Details  Subjects must NOT meet any of the following exclusion criteria
1 Symptoms related to diagnosis of complicated appendicitis (if current diagnosis) for less than 24 hrs prior to current hospitalization
2 Previously hospitalized or admitted to a healthcare facility (including nursing or convalescent home) within the last 6 months
3 Managed by Staged Abdominal Repair or other open abdomen technique
4 Known at study entry to have an IAI caused by a pathogen(s) resistant to one of the study drug antibiotics
5 Acute Physiology and Chronic Health Evaluation (APACHE) II score grater than25
6 Considered unlikely to survive the 6-8 week study period
7 Any rapidly-progressing disease or immediately life-threatening illness, including acute hepatic failure, respiratory failure and septic shock
8 Requirement for vasopressors at therapeutic dosages (ie dopamine greater than 5 μgperkgpermin, any dose of norepinephrine, epinephrine or phenylephrine) to maintain a systolic blood pressure greater than or equal to90 mm Hg or a mean arterial pressure greater than or equal to 70 mm Hg
9 Renal failure as defined as
a Threefold increase of serum creatinine to a known previous value or
b Decrease in estimated glomerular filtration rate greater than 75 percent to a known previous value or
c Urine output of less than 0.3 mL per kg per h for greater than 24 h or
d Anuria for greater than 12 h or
e Serum creatinine of greater than 4 mg per dL (353.6 μmol per L) with an acute rise of 0.5 mg per dL (42.2 μ mol per L) compared with a previous value
10 Creatinine clearance less than 30 mLpermin as estimated by the Cockcroft-Gault equation (140-age[years]) (Body Weight in kg) (0.85 if female) per (72 Serum Creatinine [mg per dL]); or requires peritoneal dialysis, hemodialysis, or hemofiltration
11 Presence or possible signs of hepatic disease:
a Alanine aminotransferase or aspartate aminotransferase greater than 3 x upper limit of normal (ULN) or
b Total bilirubin greater than 3 x ULN unless isolated hyperbilirubinemia is directly related to the acute process
or
c Alkaline phosphatase greater than 3 x ULN or
d Subjects with diagnosis of hepatic failure
12 Hematocrit less than 25 percent or hemoglobin less than 8 g per dL
13 Neutropenia with absolute neutrophil count less than 1000 per cubic mm
14 Platelet count less than 50000 per cubic mm
15 Coagulation tests greater than 1.5 x ULN (that is prothrombin time partial thromboplastin time, or international normalized ratio)
16 Patient on anticoagulants prior to hospitalization (excluding low dose aspirin therapy)
17 Immunocompromised condition, including known HIV positivity or AIDS organ (bone marrow) transplant recipients, and hematological malignancy. Immunosuppressive therapy, including use of high-dose corticosteroids
(eg greater than 40 mg prednisone or equivalent per day for greater than 2 weeks)
18 History of moderate or severe hypersensitivity reactions to tetracyclines carbapenems or β-lactam antibiotics
19 Participation in any investigational drug or device study within 30 days prior to study entry
20 Known or suspected current central nervous system (CNS) disorder that may predispose to seizures or lower seizure threshold (eg severe cerebral arteriosclerosis epilepsy)
21 Previously received TP-434 in a clinical trial
22 Antibiotic-related exclusions
aMore than 24 hours duration of systemic antibiotic coverage for current condition (24 hrs duration as defined by recommended dosing interval in the respective prescribing information)
b Received ertapenem or any other carbapenem or tigecycline for the current infection
c Need for concomitant systemic antimicrobial agents other than study drug (including female subjects with clinical diagnosis of pelvic inflammatory disease)
d Known to have received systemic antibiotics (oral or IV) in the last 3 months (except for current acute condition or single dose such as for dental prophylaxis)
23 Refusal of mechanical ventilation, dialysis or hemofiltration, cardioversion or any other resuscitative measures and drugperfluid therapy at time of consent
24 Known or suspected inflammatory bowel disease or associated visceral abscess
 
 
Method of Generating Random Sequence   Computer generated randomization 
Method of Concealment   Centralized 
Blinding/Masking   Double Blind Double Dummy 
Primary Outcome  
Outcome  TimePoints 
Compare clinical response at the test-of-cure (TOC) visit in the microbiologically evaluable (ME) population for
subjects in the 3 treatment arms 
primary endpoint of this study will be clinical response at the TOC visit that is 10-14 days after last dose of study drug 
 
Secondary Outcome  
Outcome  TimePoints 
1) Compare clinical response for subjects in the 3 treatment arms in the following populations
a) Modified intent-to-treat (MITT)
b) Clinically modified intent-to-treat(c-MITT)
c) Microbiologically modified intent-to-treat(m-MITT)
d)Clinically evaluable (CE) e)Microbiologically evaluable (ME) (EOT and FU)
2)Compare microbiologic response at EOT and TOC in
a) m-MITT,CE,ME population
b) To assess safety & tolerability of TP-434 administration
c) (PK) parameters after TP-434 infusion 
Clinical response at end-of-treatment (EOT that is Day 4-7) , TOC( Day 10-14 after last dose of study drug), and follow-up (FU that is 28-42 days after last dose of study drug) visits
1. Microbiologic response for subjects in the 3 treatment arms at EOT and TOC visits
2. Safety endpoints:
a) Physical exam including vital signs
b) Adverse events
c) ECGs at specified time points
d) Safety Labs at specified time points 
 
Target Sample Size   Total Sample Size="150"
Sample Size from India="45" 
Final Enrollment numbers achieved (Total)= "Applicable only for Completed/Terminated trials"
Final Enrollment numbers achieved (India)="Applicable only for Completed/Terminated trials" 
Phase of Trial   Phase 2 
Date of First Enrollment (India)   28/10/2011 
Date of Study Completion (India) Applicable only for Completed/Terminated trials 
Date of First Enrollment (Global)  28/06/2011 
Date of Study Completion (Global) Applicable only for Completed/Terminated trials 
Estimated Duration of Trial   Years="1"
Months="4"
Days="0" 
Recruitment Status of Trial (Global)
Modification(s)  
Open to Recruitment 
Recruitment Status of Trial (India)  Open to Recruitment 
Publication Details   none 
Individual Participant Data (IPD) Sharing Statement

Will individual participant data (IPD) be shared publicly (including data dictionaries)?  

Brief Summary  

The proposed clinical trial is a Phase 2, randomized, double-blind, double-dummy, multicenter, prospective study to assess the efficacy, safety, and pharmacokinetics of 2 dose regiments of TP‑434 (1.5 mg/kg every 24 hours [q24h], or 1.0 mg/kg every 12 hours [q12h]) compared with Ertapenem (1g q24h) in adult community-acquired complicated intra-abdominal infections.  Randomization to the TP-434 (1.5 mg/kg q24h), TP-434 (1.0 mg/kg q12h), or ertapenem (1g q24h) treatment arms will occur in a 2:2:1 ratio. TP-434 is a novel antibiotic of the tetracycline class. The in vitro and in vivo potency and spectrum of TP-434 makes it an excellent candidate as a new single therapy treatment option for serious nosocomial infections, including skin, respiratory, urinary tract, and intra-abdominal infections.Bulgaria, India, Lithuania, Romania, and USA are the participating countries.

 
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