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CTRI Number  CTRI/2019/08/020764 [Registered on: 19/08/2019] Trial Registered Prospectively
Last Modified On: 10/09/2023
Post Graduate Thesis  No 
Type of Trial  Interventional 
Type of Study   Drug 
Study Design  Other 
Public Title of Study   A randomized, phase 2 study to evaluate Safety, Tolerability and pharmacokinetics of Itraconazole as Dry Powder for Inhalation in adult Asthmatic Patients 
Scientific Title of Study   A Randomized, Double-Blind, Multicenter, Placebo-Controlled, Phase 2 Study to Evaluate the Safety, Tolerability, and Pharmacokinetics of Itraconazole Administered as a Dry Powder for Inhalation (PUR1900) in Adult Asthmatic Patients With Allergic Bronchopulmonary Aspergillosis 
Trial Acronym   
Secondary IDs if Any  
Secondary ID  Identifier 
2018-002949-11  EudraCT 
601-0014 dated 06Feb2019  Protocol Number 
NCT03960606  ClinicalTrials.gov 
version 2.0 Amendment 1 dated 06/02/2019   DCGI 
 
Details of Principal Investigator or overall Trial Coordinator (multi-center study)  
Name   
Designation   
Affiliation   
Address 




 
Phone    
Fax    
Email    
 
Details of Contact Person
Scientific Query
 
Name  Rashmi Chitgupi 
Designation  Associate Director - Clinical Management 
Affiliation  PPD Pharmaceutical Development India Private Limited 
Address  PPD Pharmaceutical Development India Private Limited, 101, A Wing, Fulcrum, Hiranandani Business Park Sahar Road, Andheri East,Mumbai
101, A Wing, Fulcrum, Hiranandani Business Park Sahar Road, Andheri East,Mumbai
Mumbai
MAHARASHTRA
400099
India 
Phone  912266022900  
Fax  912266022999  
Email  Rashmi.Chitgupi@ppdi.com  
 
Details of Contact Person
Public Query
 
Name  Rashmi Chitgupi 
Designation  Associate Director - Clinical Management 
Affiliation  PPD Pharmaceutical Development India Private Limited 
Address  PPD Pharmaceutical Development India Private Limited. 101, A Wing, Fulcrum, Hiranandani Business Park Sahar Road, Andheri East, Mumbai
101, A Wing, Fulcrum, Hiranandani Business Park Sahar Road, Andheri East, Mumbai
Mumbai
MAHARASHTRA
400099
India 
Phone  912266022900  
Fax  912266022999  
Email  Rashmi.Chitgupi@ppdi.com  
 
Source of Monetary or Material Support  
Pulmatrix, Inc. 99 Hayden Avenue, Suite 390 Lexington, MA 02421 
 
Primary Sponsor  
Name  Pulmatrix Inc 
Address  99 Hayden Avenue, Suite 390 Lexington, MA 02421 
Type of Sponsor  Pharmaceutical industry-Global 
 
Details of Secondary Sponsor  
Name  Address 
PPD Pharmaceuticals Development India Private Limited  101, A Wing, Fulcrum, Hiranandani Business Park, Sahar Road, Andheri East, Mumbai 400099, Maharashtra, India 
 
Countries of Recruitment     Australia
India
Poland
United Kingdom
United States of America  
Sites of Study  
No of Sites = 4  
Name of Principal Investigator  Name of Site  Site Address  Phone/Fax/Email 
Dr Ritesh Agarwal  Post Graduate Institute of Medical Education and  New Outpatient Block, 3rd Floor, Sector 12
Chandigarh
CHANDIGARH 
9478402976

agarwal.ritesh@outlook.in 
Dr Narendra Khippal  Sawai Man Singh (SMS) Medical College and Hospital  Department of Clinical Research, Ground Floor, Institute of Respiratory Diseases, B-2 Subhash Nagar Shopping Center, Shastri Nagar
Jaipur
RAJASTHAN 
9829017619

drnkhippal@rediffmail.com 
Dr Akash Balki  Shree Hospital and Critical Care Centre -Hospital  3rd Floor, Clinical Research Room, Shree Hospital Unit, 799, Om Nagar, Opp Tajshree Building,Sakkardara Square
Nagpur
MAHARASHTRA 
9890812215

akash_balki@yahoo.com 
Dr Venkata Nagarjuna Maturu  Yashoda Hospital  Department of Clinical Research, Room No 310, B- Block, Rajbhavan Road, Somajiguda
Hyderabad
TELANGANA 
9100935638

arjunjipmer@yahoo.co.in 
 
Details of Ethics Committee  
No of Ethics Committees= 4  
Name of Committee  Approval Status 
Institutional Ethics Committee  Submittted/Under Review 
Institutional Ethics Committee  Submittted/Under Review 
Institutional Ethics Committee  Approved 
Shree Hospital Ethics Committee  Approved 
 
Regulatory Clearance Status from DCGI  
Status 
Approved/Obtained 
 
Health Condition / Problems Studied  
Health Type  Condition 
Patients  (1) ICD-10 Condition: B441||Other pulmonary aspergillosis,  
 
Intervention / Comparator Agent  
Type  Name  Details 
Comparator Agent  Placebo  Placebo will be administered orally using Dry Powder Inhaler, once daily for 28 days 
Intervention  PUR1900  10mg, 20mg or 35mg of PUR1900 using Dry Powder Inhaler, once daily for 28 days  
 
Inclusion Criteria  
Age From  18.00 Year(s)
Age To  75.00 Year(s)
Gender  Both 
Details  1. Can provide written informed consent before the performance of any study-specific
procedures.
2. Is a male or female, 18 to 75 years old (inclusive) at the time of signing the informed
consent.
3. Has a body mass index of 18.0 to 35.0 kg/m2 (inclusive) at screening.
4. Has a historical diagnosis of asthma, as per the Global Initiative for Asthma (GINA)
2018 update.
5. Has a confirmed historical diagnosis of ABPA, as per the Modified International Society for Human and Animal Mycology (ISHAM) working group 2013 criteria.
6. Is currently considered to be in one of the following stages of ABPA: Stage 2
(Response), Stage 4 (Remission), Stage 5a (Treatment-dependent ABPA), or Stage 5b
(Glucocorticoid-dependent asthma) (Section 13.3).
7. Has a serum immunoglobulin (Ig) E ≥1000 IU/mL during screening (Visit 1 or Visit 2).
8. Can perform a valid, reproducible spirometry test with demonstration of a prebronchodilator FEV1 ≥50% of predicted normal for age, sex, race, and height at a screening visit.
9. Has a documented stable asthma medication regimen during screening (Day -28 to
Day 1), including SABA, LABA, and LTRA use and inhaled and/or oral GCS.
10. Subjects who are sexually active, male subjects able to father a child, and female
subjects of childbearing potential must agree to follow the contraception requirements
outlined in Section 5.8.4 of this protocol.
11. Can demonstrate the correct inhalation technique for the use of the delivery device at
screening and before dosing on Day 1.
12. Is willing and able to comply with all study procedures and assessments, including
scheduled visits, drug dosing plan, study procedures, laboratory tests, and study
restrictions. 
 
ExclusionCriteria 
Details  1. Has used any anti-IgE (eg, Xolair® [omalizumab]) or anti-interleukin-5 (IL-5)
biologics (eg, Cinqair® [reslizumab], Nucala® [mepolizumab], or Fasenra® [benralizumab]) in the 6 months before first dose of study drug.
2. Is a female of childbearing potential who is pregnant or lactating or who plans to
become pregnant during the study (all female subjects must have a negative pregnancy
test at screening and predose on Day 1). A woman is considered to be of childbearing
potential unless she is either permanently sterile (hysterectomy, bilateral
salpingectomy, bilateral oophorectomy, bilateral tubal occlusion/ligation) or
postmenopausal (had no menses for 12 months without an alternative medical cause).
3. Is taking or has taken any prescribed or over-the-counter (OTC) drug that is a CYP3A4 inhibitor or substrate in the 14 days (or 5 half-lives, whichever is longer) before first dose of study drug and for the duration of the study (exclusion also applies to the whole fruit or juices of grapefruit and Seville or pomelo oranges).
4. Is taking or has taken any herbal remedies or CYP3A4 inducers in the 28 days before first dose of study drug.
5. Has used any systemic azole antifungal agent in the 6 months before first dose of study drug.
6. Has a history of life-threatening asthma within the last 5 years, defined as an asthma
episode that required intubation and/or was associated with hypercapnia, respiratory
arrest, and/or hypoxic seizures.
7. Had an occurrence of asthma or ABPA exacerbations within the 28 days before screening or during the 28-day period before Day 1.
8. Had an occurrence of clinically significant bacterial, viral, or fungal infection that
required systemic (oral or intravenous) antibiotics, antivirals, or antifungals within the
28 days before screening. Topical treatments, other than antifungals, are allowed.
9. Received any investigational medical product in a clinical research study within the
previous 3 months before dosing in this study.
10. Is a study site employee, an immediate family member of a study site employee, or a
sponsor employee.
11. Has previously received PUR1900.
12. Has a history of any significant drug or alcohol abuse in the past 2 years before
screening, as judged by the investigator.
13. Has current tobacco or inhaled marijuana use or history of smoking tobacco or marijuana within the last 6 months before screening.
14. Is a current user of e-cigarettes or has used these products within the last 6 months before screening.
15. Has the absence of suitable veins for multiple venipunctures/cannulation as assessed
by the investigator or designee at screening.
16. Has evidence or history of clinically significant abnormal serum chemistry, hematology, or urinalysis at screening, as judged by the investigator (particularly elevation of liver enzymes or bilirubin).
17. Has a positive urine test result for drugs of abuse, alcohol, or cotinine at screening (unless, in the opinion of the investigator, this can be explained by the subject’s current medications).
18. Has a positive human immunodeficiency virus (HIV; type A and type B) antibody result: a subject who is HIV antibody positive is not excluded if a subsequent CD4 count is ≥200 cells/μL.
19. Has evidence or a history of clinically significant cardiovascular, renal, hepatic, or
gastrointestinal disease or neurological or psychiatric disorder, as judged by the investigator.
20. Has evidence or a history of endocrine, immunological, or autoimmune disease that
would affect the subject’s safety or confound the assessment of study endpoints in the opinion of the investigator.
21. Has a current diagnosis of any chronic airway disease other than asthma, ABPA, or bronchiectasis believed to be related to ABPA, such as chronic obstructive pulmonary disease, pulmonary fibrosis, cystic fibrosis, or Churg-Strauss syndrome. A subject whose predominating clinical disease burden is related to bronchiectasis (eg, a subject with 2 or more infective exacerbations of bronchiectasis in the past 12 months or a subject with chronic colonization with Pseudomonas aeruginosa) will be excluded.
22. Has evidence of ventricular dysfunction, such as congestive cardiac failure, or a history of congestive cardiac failure. N-terminal pro B-type natriuretic peptide (NT-pro BNP) will be checked at screening only. A subject with a confirmed value of >400 pg/mL will not be eligible to participate.
23. Has a 12-lead ECG demonstrating a mean QT interval corrected by the Fridericia
formula (QTcF) >450 ms for a male subject or >470 ms for a female subject at screening. A repeat triplicate ECG is allowed if a mean QTcF >450 ms is recorded at Visit 1 or Visit 2.
24. Has a serious adverse reaction or serious hypersensitivity to any of the formulation
excipients.
25. Has a history of allergies to or hypersensitivity reactions after dosing of itraconazole or other antifungal azoles.
26. Had a major trauma or surgery within the last 28 days before screening.
27. Has a planned or elective surgery, hospitalizations, or participation in other
interventional studies any time during the study that may interfere with study logistics
or safety.
28. Has donated or had a loss of greater than 400 mL of blood within the 3 months before
screening.
29. Has the presence of hoarseness or oropharyngeal candidiasis at screening.
30. Has other social, psychiatric, surgical, or medical conditions or screening laboratory
abnormalities that may increase the risk associated with study participation or may
interfere with the interpretation of study results and, in the judgement of the investigator, would make the subject inappropriate for entry into the study. 
 
Method of Generating Random Sequence   Computer generated randomization 
Method of Concealment   Centralized 
Blinding/Masking   Double Blind Double Dummy 
Primary Outcome  
Outcome  TimePoints 
The Primary outcome to evaluate safety and tolerability will be measured by:
• Incidence of treatment-emergent adverse
events
• Incidence of intraday FEV1 declines (from predose to postdose) of ≥10%, ≥15%, and ≥20%
• Vital sign measurements (respiratory rate,
blood pressure, heart rate, oxygen saturation
by pulse oximetry, oral or tympanic
temperature)
• Physical examination findings
• Clinical laboratory parameters
• 12-Lead electrocardiogram findings 
Time Frame: From Day 1 through Follow Up (which is 7 to 10 days after the last dose) 
 
Secondary Outcome  
Outcome  TimePoints 
To characterize the pharmacokinetics of multiple
doses of inhaled PUR1900 in plasma and sputum 
Pharmacokinetic parameters of itraconazole and hydroxy-itraconazole in plasma,
including, but not limited to, Cmax, Tmax, AUC, CL/F, and Vz/F

Sputum concentrations of itraconazole and hydroxy-itraconazole on Day 2, on Day 14, and at follow-up 
To evaluate the effect of PUR1900 on biomarkers of inflammation (sputum eosinophils and serum IgE)  Change from baseline (Day -9 to Day -6) to Day 28 in sputum eosinophil count

Change from baseline (Day -9 to Day -6) to Day 28 in percentage of sputum eosinophils

Change from baseline (Day 1) to Day 28 in IgE plasma concentration 
To evaluate the effect of PUR1900 on pulmonary
function following single- and multiple-dose
administration of PUR1900 
Change from baseline (Day 1) to Day 28 in FEV1

Change from baseline (Day 1) to Day 28 in FVC, PEF, and FEV1/FVC 
To evaluate the impact of PUR1900 on respiratory symptoms, as measured by the ACQ-6  Change from baseline (Day 1) to Day 28 in ACQ-6 score 
To evaluate the effect of PUR1900 on the
Aspergillus fumigatus burden in the sputum, as
assessed by quantitative PCR and sputum culture 
Change from baseline (Day 1) to Day 28 in A fumigatus burden in sputum as assessed by quantitative PCR and sputum culture 
 
Target Sample Size   Total Sample Size="64"
Sample Size from India="12" 
Final Enrollment numbers achieved (Total)= "Applicable only for Completed/Terminated trials"
Final Enrollment numbers achieved (India)="Applicable only for Completed/Terminated trials" 
Phase of Trial   Phase 2 
Date of First Enrollment (India)   26/08/2019 
Date of Study Completion (India) Applicable only for Completed/Terminated trials 
Date of First Enrollment (Global)  31/05/2019 
Date of Study Completion (Global) Applicable only for Completed/Terminated trials 
Estimated Duration of Trial   Years="0"
Months="2"
Days="15" 
Recruitment Status of Trial (Global)
Modification(s)  
Closed to Recruitment of Participants 
Recruitment Status of Trial (India)  Closed to Recruitment of Participants 
Publication Details   NIL 
Individual Participant Data (IPD) Sharing Statement

Will individual participant data (IPD) be shared publicly (including data dictionaries)?  

Brief Summary  

This is a study in Adult Asthmatic Patients with Allergic Bronchopulmonary Aspergillosis.

This is randomized, double-blind, multicenter and placebo controlled, multiple-arm study. Following the screening and confirmation of eligibility, the subjects will be randomized (1:1:1:1) into 4 arms of 16 subjects each and will receive the 10mg, 20mg, or 35 mg of PUR1900 or Placebo, administered via dry powder inhalation daily for 28 days.

 
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