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CTRI Number  CTRI/2019/07/019996 [Registered on: 02/07/2019] Trial Registered Prospectively
Last Modified On: 31/12/2019
Post Graduate Thesis  No 
Type of Trial  Interventional 
Type of Study   Vaccine 
Study Design  Single Arm Study 
Public Title of Study   A clinical study to assess the long-term immune response after primary vaccination and immune response after re-vaccination with typhoid conjugate vaccine 
Scientific Title of Study   A prospective, open-label, multicenter, phase IV clinical study to evaluate the long term persistence of immunological response after primary vaccination with Typhoid Vi Conjugate Vaccine and response to booster dose vaccination with Typhoid Vi Conjugate Vaccine of M/s Cadila Healthcare Limited in healthy subjects 
Trial Acronym  None 
Secondary IDs if Any  
Secondary ID  Identifier 
18-02; Version No. 00; Dated 27/10/2018  Protocol Number 
 
Details of Principal Investigator or overall Trial Coordinator (multi-center study)  
Name  Dr Ravindra Mittal 
Designation  Medical Advisor & Head - Regulatory Affairs 
Affiliation  Cadila Healthcare Ltd 
Address  Cadila Healthcare Ltd, Sigma CommerZone, Near ISCON Temple & Satellite Cross Roads, Ambli-Bopal Road, Ahmedabad

Ahmadabad
GUJARAT
380015
India 
Phone  079-26868926  
Fax  079-26868910  
Email  r.mittal@zyduscadila.com  
 
Details of Contact Person
Scientific Query
 
Name  Dr Ravindra Mittal 
Designation  Medical Advisor & Head - Regulatory Affairs 
Affiliation  Cadila Healthcare Ltd 
Address  Cadila Healthcare Ltd, Sigma CommerZone, Near ISCON Temple & Satellite Cross Roads, Ambli-Bopal Road, Ahmedabad

Ahmadabad
GUJARAT
380015
India 
Phone  079-26868926  
Fax  079-26868910  
Email  r.mittal@zyduscadila.com  
 
Details of Contact Person
Public Query
 
Name  Dr Pavankumar Daultani 
Designation  Senior Manager – Medical & Regulatory Affairs 
Affiliation  Cadila Healthcare Ltd 
Address  Cadila Healthcare Ltd, Sigma CommerZone, Near ISCON Temple & Satellite Cross Roads, Ambli-Bopal Road, Ahmedabad

Ahmadabad
GUJARAT
380015
India 
Phone  079-26868937  
Fax  079-26868910  
Email  pavankumar.daultani@zyduscadila.com  
 
Source of Monetary or Material Support  
Cadila Healthcare Ltd., Sigma CommerZone, Near ISCON Temple & Satellite Cross Roads, Ambli-Bopal Road, Ahmedabad – 380015, Gujarat, India 
 
Primary Sponsor  
Name  Cadila Healthcare Ltd 
Address  Sigma CommerZone, Near ISCON Temple & Satellite Cross Roads, Ambli-Bopal Road, Ahmedabad – 380015, Gujarat, India 
Type of Sponsor  Pharmaceutical industry-Indian 
 
Details of Secondary Sponsor  
Name  Address 
Nil  NA 
 
Countries of Recruitment     India  
Sites of Study
Modification(s)  
No of Sites = 8  
Name of Principal Investigator  Name of Site  Site Address  Phone/Fax/Email 
Dr K Siva Ram Prasad  Gandhi Medical College & Hospital, Secunderabad  Study room, 1st floor, main hospital building, Department of Pediatrics, Gandhi Hospital, Musheerabad, Secunderabad – 500003
Hyderabad
TELANGANA 
9440424545

sivaram175@yahoo.com 
Dr Ambrose Kumar Kandulna  GCS Medical College, Hospital and Research Centre  Room No. 13, Medicine OPD, Department of Medicine, GCS Medical College, Hospital and Research Centre, Opp. DRM office, Near Chamunda bridge, Naroda road, Ahmedabad – 380025
Ahmadabad
GUJARAT 
7698059533

drambrosek@gmail.com 
Dr Sanjay Kumar Jangid  Hi-Tech Medical College & Hospital  Medicine OPD, Department of Medicine, Hi-Tech Hospital, Health Park, Pandara, Rasulgarh, Bhubaneswar -751025
Khordha
ORISSA 
9337671521

drsanjay_jangid@yahoo.co.in 
Dr M Ravi Kumar  Indo-US Superspeciality Hospital  Room No. 2, Ground floor, Indo-US Superspeciality Hospital, Shyam Karan Road, Anand Bagh, Ameerpet, Hyderabad – 500016
Hyderabad
TELANGANA 
8790923937

mallelli99@gmail.com 
Dr Kheya Ghosh Uttam  Institute of Child Health  Room No. 113, Ground floor, Institute of Child Health, 11, Dr. Biresh Guha Street, Kolkata – 700017
Kolkata
WEST BENGAL 
9831194865

kheyauttam@yahoo.co.in 
Dr Vimal Kant Goyal  Panchsheel Hospital Pvt Ltd   Pediatric OPD, Ground floor, Panchsheel Hospital Pvt Ltd, Opp. Gokul puri police station, Yamuna Vihar, Delhi - 110053
New Delhi
DELHI 
9810106800

vkgoyal22@gmail.com 
Dr Uma Nayak  Parul Sevashram Hospital  Room No. 15, Ground floor, Department of Pediatrics, Parul Sewashram Hospital, Parul Institute of Medical Sciences & Research, Limda, Waghodia, Vadodara – 391760
Vadodara
GUJARAT 
9327214065

umasnayak@gmail.com 
Dr Shrikant Sharma  SMS Medical College and Hospital, Jaipur  Medicine Unit VII, 2nd floor, Hospital building, Department of Medicine, SMS Medical College and Hospital, JLN Marg, Jaipur-302004
Jaipur
RAJASTHAN 
9214403945

skant_sunita@yahoo.co.in 
 
Details of Ethics Committee
Modification(s)  
No of Ethics Committees= 8  
Name of Committee  Approval Status 
Ethics Committee, Institute of Child Health, 11, Dr. Biresh Guha Street, Kolkata – 700017, West Bengal  Approved 
Ethics Committee, SMS Medical College and Attached Hospitals, JLN Marg, Jaipur – 302004, Rajasthan  Approved 
Institutional Ethics Committee for Human Research, Parul Institute of Medical Sciences & Research, Limda, Waghodia, Vadodara – 391760, Gujarat  Submittted/Under Review 
Institutional Ethics Committee, Gandhi Medical College and Hospital, Musheerabad, Secunderabad-500003, Telangana  Approved 
Institutional Ethics Committee, GCS Medical College, Hospital & Research Centre, Opp. DRM office, Nr. Chamunda Bridge, Naroda Road, Ahmedabad – 380025, Gujarat  Approved 
Institutional Ethics Committee, Indo-US Superspeciality Hospital, Shyam Karan Road, Anand Bagh, Ameerpet, Hyderabad – 500016, Telangana  Approved 
Institutional Ethics Committee, Panchsheel Hospital, Opp. Gokul puri police station, Yamuna Vihar, Delhi - 110053  Approved 
Institutional Ethics Committee, Sparsh Hospitals and Critical Care Private Limited, Saheed Nagar, Bhubaneswar - 751007, Odisha  Approved 
 
Regulatory Clearance Status from DCGI  
Status 
Approved/Obtained 
 
Health Condition / Problems Studied  
Health Type  Condition 
Healthy Human Volunteers  Immunization against typhoid fever 
 
Intervention / Comparator Agent  
Type  Name  Details 
Comparator Agent  Not applicable  Not applicable 
Intervention  Typhoid Vi Conjugate Vaccine of M/s Cadila Healthcare Ltd  The subjects whose baseline anti-Vi IgG antibody titre is below the proposed seroprotective cut-off will be considered eligible to receive a single dose (0.5 ml) of the vaccine at visit 2 (day 0). The study vaccine will be administered as i.m. injection in the upper arm taking aseptic precautions 
 
Inclusion Criteria  
Age From  6.00 Month(s)
Age To  45.00 Year(s)
Gender  Both 
Details  1. Subjects who had participated and completed the previously conducted phase II/III clinical study (Project No. 15-03) and had received the study vaccine before 3 years ± 3 months
2. Informed consent from adult subjects or from parents of the pediatric subjects. Additionally, assent from pediatric subjects of ≥7 years of age
3. Adult subjects or parents of pediatric subjects literate enough to fill the diary card 
 
ExclusionCriteria 
Details  1. History of typhoid fever or vaccination against typhoid fever after previous study
2. Subjects administered blood, blood containing products or immunoglobulins during the past 3 months
3. Clinically significant systemic disorder such as cardiovascular, respiratory, neurologic, gastrointestinal, hepatic, renal, endocrine, hematological or immunological disorder
4. Subjects with confirmed or suspected immunosuppressive or immunodeficiency disorder; or subjects on any immunosuppressive or immunostimulant therapy
5. Known case of thrombocytopenia or any coagulation disorder, or subjects on anticoagulation therapy
6. Participation in another clinical trial in the past 3 months
7. Subjects with history of alcohol or drug abuse in the past one year 
 
Method of Generating Random Sequence   Not Applicable 
Method of Concealment   Not Applicable 
Blinding/Masking   Not Applicable 
Primary Outcome  
Outcome  TimePoints 
-Long-term persistence of anti-Vi IgG antibodies
-Seroconversion rate 28 days post-vaccination 
Day 0 and Day 28 
 
Secondary Outcome  
Outcome  TimePoints 
-Seroconversion rate 10 days post-vaccination
-Geometric mean titre of anti-Vi IgG antibodies 10 days and 28 days post-vaccination as compared to the baseline 
Day 10 and Day 28 
 
Target Sample Size   Total Sample Size="236"
Sample Size from India="236" 
Final Enrollment numbers achieved (Total)= "112"
Final Enrollment numbers achieved (India)="112" 
Phase of Trial   Phase 4 
Date of First Enrollment (India)   15/07/2019 
Date of Study Completion (India) 20/11/2020 
Date of First Enrollment (Global)  Date Missing 
Date of Study Completion (Global) Date Missing 
Estimated Duration of Trial   Years="1"
Months="0"
Days="0" 
Recruitment Status of Trial (Global)
Modification(s)  
Not Applicable 
Recruitment Status of Trial (India)  Completed 
Publication Details   Not publication yet 
Individual Participant Data (IPD) Sharing Statement

Will individual participant data (IPD) be shared publicly (including data dictionaries)?  

Brief Summary  

This phase IV clinical trial is an extension of previously conducted phase II/III clinical trial (Protocol No. 15-03; CTRI Registration No. CTRI/2016/05/006975). A total of 236 subjects who were enrolled (aged 6 months to 45 years at the time of enrollment) and who had completed the previous clinical trial will be enrolled in the current study around 3 years after their primary vaccination. This study is planned to evaluate the long-term persistence of anti-Vi IgG antibodies in the subjects who were vaccinated with Typhoid Vi conjugate vaccine in the previous clinical trial and also to evaluate the immunogenicity and safety of booster dose vaccination with Typhoid Vi conjugate vaccine I.P. of M/s Cadila Healthcare Ltd. The anti-Vi antibody titres will be evaluated using VaccZyme ELISA kits. After booster dose vaccination, seroconversion rate (defined as ≥4-fold rise in antibody titre as compared to the baseline titre) and geometric mean titre of antibodies will be evaluated at day 10 & day 28. The safety of the vaccine will be assessed by recording the adverse events occurring to the vaccinated subjects .

 
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