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CTRI Number  CTRI/2018/10/015923 [Registered on: 08/10/2018] Trial Registered Prospectively
Last Modified On: 03/12/2019
Post Graduate Thesis  No 
Type of Trial  Interventional 
Type of Study   Drug 
Study Design  Other 
Public Title of Study   A clinical trial study to assess the efficacy and safety of Granexin Gel in the treatment of Diabetic Foot Ulcer 
Scientific Title of Study   Phase 3, Randomized, Double-blind, Parallel-group, Vehicle controlled, Multicenter Study of the Efficacy and Safety of Granexin Gel in the Treatment of Diabetic Foot Ulcer  
Trial Acronym  GAIT 1 
Secondary IDs if Any
Modification(s)  
Secondary ID  Identifier 
2015-DFU-301, Version 7.0 dated 13 Jun 2019  Protocol Number 
 
Details of Principal Investigator or overall Trial Coordinator (multi-center study)  
Name   
Designation   
Affiliation   
Address 




 
Phone    
Fax    
Email    
 
Details of Contact Person
Scientific Query
 
Name  Dr Sonika Newar 
Designation  Senior Medical Monitor 
Affiliation  JSS Medical Research India Private Limited 
Address  6th Floor, Tower B, Plot 12/2, Vatika Mindscapes, Sector 27D

Faridabad
HARYANA
121003
India 
Phone  8800799887  
Fax  01296613520  
Email  sonika.newar@jssresearch.com  
 
Details of Contact Person
Public Query
 
Name  Dr Shariq Anwar 
Designation  Head Operations 
Affiliation  JSS Medical Research India Private Limited 
Address  6th Floor, Tower B, Plot 12/2, Vatika Mindscapes, Sector 27D

Faridabad
HARYANA
121003
India 
Phone  9810979215  
Fax  01296613520  
Email  shariq.anwar@jssresearch.com  
 
Source of Monetary or Material Support  
FirstString Research, Inc. 300 West Coleman Blvd. Suite 203 Mount Pleasant, SC 29464  
 
Primary Sponsor  
Name  FirstString Research Inc 
Address  300 West Coleman Blvd. Suite 203 Mount Pleasant, SC 29464  
Type of Sponsor  Pharmaceutical industry-Global 
 
Details of Secondary Sponsor  
Name  Address 
NIL  NIL 
 
Countries of Recruitment     India
Canada
Czech Republic
Hungary
Poland
United States of America  
Sites of Study
Modification(s)  
No of Sites = 19  
Name of Principal Investigator  Name of Site  Site Address  Phone/Fax/Email 
Dr Shailesh Parikh  Aman Hospital & Research Centre  Clinical Research Department, Ground Floor, Aman Hospital & Research Centre, 15 Shaswat, opposite ESI Hospital
Vadodara
GUJARAT 
9426007550

parikhdrshailesh@gmail.com 
Dr Bhavin H Patel  Anand Multispecialty Hospital  ‘B’ Tower, Sundarvan Complex, Beside IOCL Petrol Pump, Near Gorwa ITI,
Vadodara
GUJARAT 
9879617765

drbhavinvithani@gmail.com 
Dr Poorna Gopal Azad  Anu Hospital  Kovelamudivari Street, Suryarapoet, Vijayawada- 520002
Krishna
ANDHRA PRADESH 
9866142192

drpurnagopal@gmail.com 
Dr Vipul Sud  Batra Hospital and Medical Research Centre  Academics Research Dept. First Floor Batra Hospital and Medical Research Centre, 1, Tughlakabad Institutional Area, Mehrauli Badarpur Road, Near Saket Metro Station
New Delhi
DELHI 
9810524493

sudvipul@gmail.com 
Dr Brij Mohan  Brij Medical Centre Pvt. Ltd.  Room No. 9, Ground Floor, Brij Medical Centre Pvt Ltd., 94 E, Near Panki Police Station, Gangaganj Colony, Panki, Kanpur
Kanpur Nagar
UTTAR PRADESH 
8263833668

drbrijmohan@bmchospital.in 
Dr Sandeep Julka  CHL-Hospitals [Unit of Convenient Hospitals Ltd.,]  Near L.I.G. Square, A.B. Road,
Indore
MADHYA PRADESH 
9977999687

drsunilmahavarsms@gmail.com 
Dr K P Singh  Fortis Hospital, Mohali  Room no.- B020, Ground floor, OPD Block Fortis Hospital, Ground Floor, Endocrinology Department, Phase VIII, Sector 62, Mohali
Fatehgarh Sahib
PUNJAB 
9815311711

drkp1292@gmail.com 
Dr Vikrant Ghattanatti  KLE’s Dr. Prabhakar Kre Hospital  Site Management Office, 2nd Floor, Sharavati Ward, KLEs Dr. Prabakar Kore Hospital and MRC, NH Service Rd, Nehru Nagar
Belgaum
KARNATAKA 
9844445598

victorvikrant@gmail.com 
Dr Sanjeev Mohan  KRM Hospital and Research Centre  OPD Room No. 02, Ground floor, KRM Hospital and Research Centre, 3/92-93 Vijayant Khand, Gomti Nagar
Lucknow
UTTAR PRADESH 
7007208089

krmhrclko@gmail.com 
Dr Dinesh Agarwal  Marwari Hospital and Research Centre  Room no. 3, 1st floor, Marwari Hospital and Research Centre, SJ Road, Athgaon, Guwahati
Kamrup
ASSAM 
9864061456

drdinesh944@gmail.com 
Dr B S Madhu  Mysore Medical College & Research Institute & Associated Hospital  2nd floor, Department of Surgery, Neurosurgical Block, Mysore Medical College & Research Institute & Associated Hospital, Irwin Road
Mysore
KARNATAKA 
9900513688

drmadhubs@gmail.com 
Dr Samir Saini  Parul Hospital of Medical Sciences and Research  PO : Limda, TA :m Waghodia,
Vadodara
GUJARAT 
7433014704

samirsaini@gmail.com 
Dr Sushil Jindal  People College of Medical Sciences and Research Centre  Room No. 1, Fourth Floor, Department of Medicine Peoples College of Medical Sciences and Research Centre Peoples Campus, Bhanpur, Bhopal
Bhopal
MADHYA PRADESH 
9300033888

susjindal@gmaoil.com 
Dr J S Kushwaha  Prakhar Hospital Pvt. Ltd.  OPD Chamber, Ground Floor, Prakhar Hospital Pvt. Ltd., 8/219 Arya Nagar, Kanpur
Kanpur Nagar
UTTAR PRADESH 
8263833668

lobe1617@gmail.com 
Dr D Prasada Rao  Rajiv Gandhi Institute of Medical Sciences & RIMS Govt. General Hospital  Srikakulum- 532001,
Srikakulam
ANDHRA PRADESH 
9849702720

drprasadaraod@gmail.com 
Dr Sandeep Kumar Mathur  S.M.S Medical College and Attached Hospitals  Room No. 42, Fourth floor, Dhanwantri OPD Block. S.M.S Medical College and Attached Hospitals. J.L.N. Marg, Near Albert Hall Museum, Ram Niwas Garden
Jaipur
RAJASTHAN 
9414048666

drsandeepmathur@rediffmail.com 
Dr Gyan Chand  Sanjay Gandhi Postgraduate Institute of Medical Sciences  Room No. 104, First Floor, Block-C, Deptt. Endocrine Surgery .Sanjay Gandhi Postgraduate Institute of Medical Sciences Rae Bareli Road
Lucknow
UTTAR PRADESH 
9451546353

drgyanchandpgi@gmai.com 
Dr Preetam K Ahirrao  Supe Heart and Diabetes Hospital and Research Centre  Clinical Research Department, First Floor, Supe Heart and Diabetes Hospital and Research Center, Near Rungta School, Gharpure Ghat, Ashok Stambh,
Nashik
MAHARASHTRA 
9860211112

gahirraopreetam@gmail.com 
Dr Lily Rodrigues  Surakshaka Multispeciality and Diabetes Hospital  4th Floor, Clinical Research Deptt., Surakshaka Multi-Speciality and Diabetes Hospital, Room No: Doctor’s Cabin, Ground Floor, M.I.G – 218, K.P.H.B Main Road, Kukatpally
Hyderabad
ANDHRA PRADESH 
9704799955

drlily@gmail.com 
 
Details of Ethics Committee
Modification(s)  
No of Ethics Committees= 19  
Name of Committee  Approval Status 
Anand Institutional Ethics Committee  Approved 
Anu Hospitals Ethics Committee  Approved 
Ethics Committee Marwari Hospital and Research Centre ECRC-HEC  Approved 
Ethics Committee, Brij Medical Centre  Approved 
Ethics Committee. S.M.S Medical College and Attached Hospitals. Jaipur   Approved 
Institutional Ethics Committee for Human Research, Parul Hospital  Submittted/Under Review 
Institutional Ethics Committee Peoples College of Medical Sciences and Research Centre  Approved 
Institutional Ethics Committee, Aman Hospital and Research Centre  Approved 
Institutional Ethics Committee, Fortis Hospital  Approved 
Institutional Ethics Committee, KLE University  Approved 
Institutional ethics committee, Mysore Medical college & Research Institute and associated hospitals, K.R Hospital  Approved 
Institutional Ethics Committee, Prakhar Hospital Pvt. Ltd  Approved 
Institutional Ethics Committee, RIMS  Approved 
Institutional Ethics Committee, Sanjay Gandhi Post Graduate Institute of Medical Sciences, (SGPGI) Bioethics Cell  Approved 
Institutional Supe Hospital Ethics Committee  Approved 
Integrity Ethic Committee,CHL Hospital  Approved 
KRM Hospital Institutional Ethics Committee  Approved 
Scientific Reserach and Ethics Review Committee, Batra Hospital  Approved 
Surakshaka Institutional Ethics Committee. Surakshaka Hospital  Approved 
 
Regulatory Clearance Status from DCGI
Modification(s)  
Status 
Approved/Obtained 
 
Health Condition / Problems Studied
Modification(s)  
Health Type  Condition 
Patients  (1) ICD-10 Condition: E118||Type 2 diabetes mellitus with unspecified complications,  
 
Intervention / Comparator Agent  
Type  Name  Details 
Intervention  Granexin  Investigational products (Granexin gel or vehicle gel) will be dispensed directly from a provided 5 gram laminate tube, and a sterile applicator will be used to apply a uniform layer of the investigational product gel, approximately 1mm thick, over the target ulcer wound. The target ulcer wound is then to be dressed using a non-adherent dressing, followed by non-occlusive dressing such as mesh gauze. The dose depends on the size of the wound, Frequency is weekly, Duration of treatment is 12 weeks 
Comparator Agent  Vehicle  Investigational products (Granexin gel or vehicle gel) will be dispensed directly from a provided 5 gram laminate tube, and a sterile applicator will be used to apply a uniform layer of the investigational product gel, approximately 1mm thick, over the target ulcer wound. The target ulcer wound is then to be dressed using a non-adherent dressing, followed by non-occlusive dressing such as mesh gauze. The dose depends on the size of the wound, Frequency is weekly, Duration of treatment is 12 weeks 
 
Inclusion Criteria  
Age From  18.00 Year(s)
Age To  85.00 Year(s)
Gender  Both 
Details  1. Age 18 years or older
2. Established diagnosis of diabetes mellitus (type I or II)
3. Glycosylated hemoglobin (HbA1c) value < 10.0% at the screening visit
4. Diagnosis of neuropathic foot ulcer by 10 g monofilament test, tuning fork, or cotton wisp
5. Designated foot ulcer meets the following criteria at both the screening and baseline visits: a. Present for at least 4 weeks
b. Full-thickness cutaneous ulcer below the ankle surface
c. University of Texas grade A1
d. Wound area (after debridement) 1 to 40.0 cm2
e. Viable, granulating wound (investigator discretion)
6. Ankle brachial index ≥ 0.7 at both the screening and baseline visits. If the ABI is > 1.30, one of the following confirmatory tests must be performed for the patient to be considered eligible:
a. Does not have a monophasic or biphasic flow (with loss of reverse flow) in the artery of the foot with the target ulcer via doppler waveform analysis of the dorsalis pedis and posterior tibial arteries, as determined by standard practices of the investigator and the site.
b. Transcutaneous oxygen pressure (TcPO2) at the foot > 40 mmHg
7. Signed informed consent
8. Female patients of childbearing potential must have a negative pregnancy test at screening and must agree to use hormonal contraceptive, intrauterine device, diaphragm with spermicide, condom with spermicide, or abstinence throughout until 2 weeks after the last administration of investigational product. Male patients must also agree to use contraception such as a condom.



 
 
ExclusionCriteria 
Details  1. Change (decrease or increase) in size of the designated target ulcer by greater than or equal to 30 percent during the 7-day screening period
2. Cannot tolerate off-loading methods or cannot comply with study related procedures
3. Has an ulcer that meets any of the following criteria:
a. Shows signs of severe clinical infection, defined as pus oozing from the ulcer site
b. Requires surgical debridement
c. Is positive for β-hemolytic streptococci upon culture performed prior to screening debridement procedure.
d. Has greater than 50 percent slough, significant necrotic tissue, bone, tendon, or capsule exposure
e. Is highly exuding (i.e., requires daily change of dressing)
4. Requires total contact cast
5. Ankle brachial pressure index less than 0.7
6. Has a local or systemic infection or local lymphangitis greater than or equal to 0.5 cm
7. Has any 1 of the following (only 1 of the 2 tests is required):
a. A monophasic or biphasic flow (with loss of reverse flow) in the artery of the foot with the target ulcer via doppler waveform analysis of the dorsalis pedis and posterior tibial arteries
b. Transcutaneous oxygen pressure (TcPO2) at the foot less than 40 mmHg
8. Presence of active malignant or benign tumors of any kind, (with exception to nonmelanoma skin cancer as per investigator’s discretion)
9. Congestive heart failure (New York Heart Association class II–IV)
10. Coronary heart disease with ST segment elevation myocardial infarction, coronary artery bypass graft, or percutaneous transluminal coronary angioplasty within the last 6 months
11. Active osteomyelitis of the foot with the target ulcer detected by x-ray, CT scan, or MRI
12. Active connective tissue disease
13. Acute or chronic Charcot neuro-arthropathy as determined by clinical and/or radiographic examination
14. Active treatment with systemic corticosteroids or topical corticosteroids (for treatment of the target ulcer or any area of the foot). This does not include inhaled corticosteroids or topical corticosteroids used for conditions other than treating the target ulcer or any area of the foot. (Wash out period for systemic corticosteroids is 14 days for inclusion in the study. Wash out
period for topical corticosteroids (for treatment of the target ulcer or any area of the foot) is 14 days for inclusion in the study)
15. Active treatment with systemic antibiotics (wash out period for systemic antibiotics is 7 days for inclusion in the study)
16. Previous or current radiation therapy to the distal lower extremity or likelihood to receive this therapy during study participation
17. Pregnant or nursing mothers
18. Uncontrolled anemia (hemoglobin < 10 g/dL in females and < 12 g/dL in males)
19. Estimated glomerular filtration rate < 25 mL/min
20. Poor nutritional status, defined as an albumin < 25 g/L (< 2,500 mg/dl)
21. Significant peripheral edema as per investigator’s discretion
22. Known inability or unavailability of a patient to complete required study visits during study participation
23. A psychiatric condition (e.g., suicidal ideation) or chronic alcohol or drug abuse problem, determined from the patient medical history, which, in the opinion of the investigator, may pose a threat to patient compliance
24. Use of a platelet-derived growth factor within 28 days before screening
25. Use of any investigational drug or therapy within 28 days before screening
26. Has any other factor which may, in the opinion of the investigator, compromise participation and/or follow-up in the study.





 
 
Method of Generating Random Sequence   Computer generated randomization 
Method of Concealment   Centralized 
Blinding/Masking   Participant and Investigator Blinded 
Primary Outcome  
Outcome  TimePoints 
To evaluate the efficacy of Granexin gel plus standard-of-care in the treatment of diabetic foot ulcer, as compared with vehicle gel plus standard-of-care and with standard-of-care treatment alone, as measured by investigator assessment of wound closure and by other method  At Week 12  
 
Secondary Outcome  
Outcome  TimePoints 
To evaluate the safety of Granexin gel plus standard-of-care in the treatment of diabetic foot ulcer, as compared with vehicle gel plus standard-of-care and with standard-of-care treatment alone, by standard methods and immunogenicity testing  Over the 12 week treatment period 
Incidence of complete wound closure   at Week 24 (Month 6) based on investigator assessment 
 
Target Sample Size   Total Sample Size="552"
Sample Size from India="232" 
Final Enrollment numbers achieved (Total)= "Applicable only for Completed/Terminated trials"
Final Enrollment numbers achieved (India)="Applicable only for Completed/Terminated trials" 
Phase of Trial   Phase 3 
Date of First Enrollment (India)
Modification(s)  
30/11/2018 
Date of Study Completion (India) Applicable only for Completed/Terminated trials 
Date of First Enrollment (Global)  18/04/2019 
Date of Study Completion (Global) Applicable only for Completed/Terminated trials 
Estimated Duration of Trial   Years="2"
Months="0"
Days="0" 
Recruitment Status of Trial (Global)   Not Yet Recruiting 
Recruitment Status of Trial (India)  Not Yet Recruiting 
Publication Details   None Yet 
Individual Participant Data (IPD) Sharing Statement

Will individual participant data (IPD) be shared publicly (including data dictionaries)?  

Brief Summary  

This study is a Phase 3, Randomized, Double-blind, Parallel-group, Vehicle controlled, Multicenter Study to evaluate the Efficacy and Safety of Granexin Gel plus standard-of-care in the treatment of diabetic foot ulcer, as compared with vehicle gel plus standard-of-care and with standard-of-care treatment alone. Each patient assigned to Granexin gel plus standard-of-care or vehicle gel plus standard-of-care will have the investigational product (i.e., double-blind Granexin gel or vehicle gel) applied to the target ulcer at Day 0 and at each subsequent visit during the treatment period (i.e., Day 3 and Week 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, and 12 visits).Study will be conducted at 43 study centers in United States, Canada, India, Poland, Czech Republic and Hungary.   

 
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