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CTRI Number  CTRI/2018/08/015476 [Registered on: 27/08/2018] Trial Registered Prospectively
Last Modified On: 31/12/2019
Post Graduate Thesis  No 
Type of Trial  Interventional 
Type of Study   Vaccine 
Study Design  Randomized, Parallel Group, Active Controlled Trial 
Public Title of Study   A clinical study to compare the immunological response and safety of two Hepatitis B vaccines in healthy adult subjects 
Scientific Title of Study   A prospective, randomized, two-arm, parallel, single-blind, active-controlled, multicentre, non-inferiority clinical study to evaluate the immunogenicity and safety of Hepatitis B Vaccine (rDNA) of M/s Cadila Healthcare Limited compared to Engerix-B of M/s GlaxoSmithKline Biologicals in healthy adult subjects 
Trial Acronym   
Secondary IDs if Any  
Secondary ID  Identifier 
15-05; Version No. 01; Dated 27/12/2017  Protocol Number 
 
Details of Principal Investigator or overall Trial Coordinator (multi-center study)  
Name  Dr Ravindra Mittal 
Designation  Medical Advisor & Head - Regulatory Affairs 
Affiliation  Cadila Healthcare Ltd 
Address  Cadila Healthcare Ltd, Sigma CommerZone, Near ISCON Temple & Satellite Cross Roads, Ambli-Bopal Road, Ahmedabad, Gujarat, India

Ahmadabad
GUJARAT
380015
India 
Phone  079-26868926  
Fax  079-26868910  
Email  r.mittal@zyduscadila.com  
 
Details of Contact Person
Scientific Query
 
Name  Dr Ravindra Mittal 
Designation  Medical Advisor & Head - Regulatory Affairs 
Affiliation  Cadila Healthcare Ltd 
Address  Cadila Healthcare Ltd, Sigma CommerZone, Near ISCON Temple & Satellite Cross Roads, Ambli-Bopal Road, Ahmedabad, Gujarat, India

Ahmadabad
GUJARAT
380015
India 
Phone  079-26868926  
Fax  079-26868910  
Email  r.mittal@zyduscadila.com  
 
Details of Contact Person
Public Query
 
Name  Dr Pavankumar Daultani 
Designation  Senior Manager – Medical & Regulatory Affairs 
Affiliation  Cadila Healthcare Ltd 
Address  Cadila Healthcare Ltd, Sigma CommerZone, Near ISCON Temple & Satellite Cross Roads, Ambli-Bopal Road, Ahmedabad, Gujarat, India

Ahmadabad
GUJARAT
380015
India 
Phone  079-26868937  
Fax  079-26868910  
Email  pavankumar.daultani@zyduscadila.com  
 
Source of Monetary or Material Support  
Cadila Healthcare Ltd., Sigma CommerZone, Near ISCON Temple & Satellite Cross Roads, Ambli-Bopal Road, Ahmedabad – 380015, Gujarat, India 
 
Primary Sponsor  
Name  Cadila Healthcare Ltd 
Address  Sigma CommerZone, Near ISCON Temple & Satellite Cross Roads, Ambli-Bopal Road, Ahmedabad – 380015, Gujarat, India 
Type of Sponsor  Pharmaceutical industry-Indian 
 
Details of Secondary Sponsor  
Name  Address 
Nil  Nil 
 
Countries of Recruitment     India  
Sites of Study  
No of Sites = 6  
Name of Principal Investigator  Name of Site  Site Address  Phone/Fax/Email 
Dr Vipul Khandelwal  Apex Hospitals Private Limited, Jaipur  Clinical Trial Unit, 1st floor, Academic block, Apex Hospitals Private Limited, SP-4 & 6, Malviya Industrial Area, Near Apex Circle, Malviya Nagar, Jaipur - 302017, Rajasthan
Jaipur
RAJASTHAN 
9829193517

dr.vipul@yahoo.co.in 
Dr Bingi Thrilok Chander  Gandhi Medical College & Hospital, Secunderabad  In-patient block, 2nd floor, Hospital building, Department of Medicine, Gandhi Medical College & Hospital, Musheerabad, Secunderabad - 500003, Telangana
Hyderabad
ANDHRA PRADESH 
9160860777

thrilokchander73@gmail.com 
Dr Vipul Prajapati  GCS Medical College, Hospital & Research Centre, Ahmedabad  Room No. 13, Medicine OPD, Department of Medicine, GCS Medical College, Hospital & Research Centre, Opp. DRM Office, Near Chamunda Bridge, Naroda Road, Ahmedabad – 380025, Gujarat
Ahmadabad
GUJARAT 
9909912551

prajapativipul1983@gmail.com 
Dr Dipti A Chand  Government Medical College & Hospital, Nagpur  Ward No. 49, Hospital building, Department of Medicine, Government Medical College and Hospital, Medical College Square, Hanuman Nagar, Nagpur – 440003, Maharashtra
Nagpur
MAHARASHTRA 
9823257601

dachand.ngp@gmail.com 
Dr Anupam Mandal  IPGMER and SSKM Hospital, Kolkata  Arsenic Clinic, 4th floor, Ronald Ross building, Department of Medicine, Institute of Post Graduate Medical Education & Research and Seth Sukhlal Karnani Memorial Hospital, 244, Acharya JC Bose Road, Kolkata - 700020, West Bengal
Kolkata
WEST BENGAL 
9434120356

mandalanupam75@gmail.com 
Dr Vivek Kumar  King George’s Medical University, Lucknow  Room No. 505, 5th floor, Kalam Centre, Department of Medicine, King George’s Medical University, Chowk, Lucknow – 226003, Uttar Pradesh
Lucknow
UTTAR PRADESH 
9415549428

drvivek2@rediffmail.com 
 
Details of Ethics Committee
Modification(s)  
No of Ethics Committees= 6  
Name of Committee  Approval Status 
Ethics Committee, IPGME&R Research Oversight Committee, Institute of Post Graduate Medical Education & Research, Office of the Dean, 5th floor, College Building, 244, Acharya JC Bose Road, Kolkata - 700020, West Bengal  Approved 
Institutional Ethics Committee, Apex Hospital Private Limited, SP-4 & 6, Malviya Industrial Area, Malviya Nagar, Jaipur - 302017, Rajasthan  Approved 
Institutional Ethics Committee, Gandhi Medical College & Hospital, Musheerabad, Secunderabad - 500003, Telangana  Approved 
Institutional Ethics Committee, GCS Medical College, Hospital & Research Centre, Opp. DRM office, Nr. Chamunda Bridge, Naroda Road, Ahmedabad - 380025, Gujarat  Approved 
Institutional Ethics Committee, Government Medical College, Nagpur – 440003, Maharashtra  Approved 
Institutional Ethics Committee, Office of Research Cell, Administrative Block, King George’s Medical University, Chowk, Lucknow – 226003, Uttar Pradesh  Approved 
 
Regulatory Clearance Status from DCGI  
Status 
Approved/Obtained 
 
Health Condition / Problems Studied
Modification(s)  
Health Type  Condition 
Healthy Human Volunteers  Active immunization for the prevention of hepatitis B disease caused by hepatitis B virus 
Patients  (1) ICD-10 Condition: Z23||Encounter for immunization,  
 
Intervention / Comparator Agent  
Type  Name  Details 
Comparator Agent  Engerix-B of M/s GlaxoSmithKline Biologicals  Subjects will receive three doses (1 ml each) of the vaccine on day 0, day 30 and day 60 respectively. The vaccine will be administered as intramuscular injection in the deltoid muscle in the upper arm taking aseptic precautions 
Intervention  Hepatitis B vaccine (rDNA) I.P. of M/s Cadila Healthcare Ltd.  Subjects will receive three doses (1 ml each) of the vaccine on day 0, day 30 and day 60 respectively. The vaccine will be administered as intramuscular injection in the deltoid muscle in the upper arm taking aseptic precautions 
 
Inclusion Criteria  
Age From  20.00 Year(s)
Age To  50.00 Year(s)
Gender  Both 
Details  1.Healthy adult subjects of either gender between 20-50 years
2.Subjects willing to give written informed consent
3.Subjects literate enough to fill the diary card
4.Subjects negative for serological markers for Hepatitis B (HBsAg, anti-HBs and anti-HBc) 
 
ExclusionCriteria 
Details  1.History of known or suspected allergy to any of the vaccine components
2.History of Hepatitis B vaccination
3.History of Hepatitis B infection or carrier state
4.Known history of thrombocytopenia or any coagulation disorder, or subjects on anticoagulation therapy
5.Subjects with confirmed or suspected immunosuppressive or immunodeficiency disorder; or subjects on any immunosuppressive or immunostimulant therapy
6.Clinically significant systemic disorder such as hepatic, cardiovascular, respiratory, neurologic, gastrointestinal, renal, endocrine, hematological or immunological disorder
7.History of any acute illness within the last 1 week
8.Subjects with febrile illness (temperature ≥38 oC) at the time of presentation
9.Subjects administered blood, blood containing products or immunoglobulins within the last 3 months or planned administration during the study
10.Any vaccine administration within the last 30 days
11.Pregnant and lactating women & female subjects not using acceptable contraceptive measures (double barrier methods, oral or injectable hormonal contraceptives or surgical sterilization)
12.Subjects with history of alcohol or drug abuse in the past one year 
 
Method of Generating Random Sequence   Computer generated randomization 
Method of Concealment   Not Applicable 
Blinding/Masking   Participant Blinded 
Primary Outcome  
Outcome  TimePoints 
Proportion of subjects with sero-protective levels of anti-HBs antibodies at the end of study  Day 90 (30 days after third dose of vaccine) 
 
Secondary Outcome  
Outcome  TimePoints 
Proportion of subjects with anti-HBs antibody titre ≥100 mIU/ml (high-responders) at the end of study  Day 90 (30 days after third dose of vaccine) 
Geometric mean titre of anti-HBs antibodies at baseline and at the end of study  Day 0 and Day 90 (30 days after third dose of vaccine) 
 
Target Sample Size   Total Sample Size="238"
Sample Size from India="238" 
Final Enrollment numbers achieved (Total)= "238"
Final Enrollment numbers achieved (India)="238" 
Phase of Trial   Phase 2/ Phase 3 
Date of First Enrollment (India)   01/04/2019 
Date of Study Completion (India) 17/10/2019 
Date of First Enrollment (Global)  Date Missing 
Date of Study Completion (Global) Date Missing 
Estimated Duration of Trial   Years="1"
Months="0"
Days="0" 
Recruitment Status of Trial (Global)
Modification(s)  
Not Applicable 
Recruitment Status of Trial (India)  Completed 
Publication Details   None yet 
Individual Participant Data (IPD) Sharing Statement

Will individual participant data (IPD) be shared publicly (including data dictionaries)?  

Brief Summary   This is a randomized, single blind, parallel group, multicentre, non-inferiority phase II/III clinical trial to evaluate the immunogenicity and safety of Hepatitis B vaccine of M/s. Cadila Healthcare Ltd. [Test vaccine] as compared to Engerix-B (Hepatitis B vaccine) of M/s. GlaxoSmithKline Biologicals [Reference vaccine] in healthy adult subjects of 20-50 years of age. A minimum of 238 subjects will be enrolled in this study and they will be allocated to receive the either study vaccine as per the central computer generated randomization plan. Two blood samples will be collected; once pre-vaccination (upto day -14; screening) & another post-vaccination (day 90; end of study) to assess the anti-HBs antibody titre through Chemiluminescent Microparticle ImmunoAssay (CMIA) method. The primary immunogenicity endpoint of this study is to demonstrate the non-inferiority of test vaccine to the reference vaccine for the proportion of subjects achieving seroprotective titres of anti-HBs antibodies (≥10 mIU/ml) post-vaccination. In addition, proportion of subjects achieving anti-HBs antibody titre of ≥100 mIU/ml (high-responders) at the end of study and geometric mean titre of anti-HBs antibodies at the screening and at the end of study will also be calculated for both the study groups. The safety of the vaccine will be assessed by recording the adverse events occurring during the entire course of the study. 
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