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CTRI Number  CTRI/2018/08/015189 [Registered on: 03/08/2018] Trial Registered Retrospectively
Last Modified On: 18/07/2018
Post Graduate Thesis  Yes 
Type of Trial  Observational 
Type of Study   Case Control Study 
Study Design  Single Arm Study 
Public Title of Study   relation between calcification in blood vessels of breast with wall thickness of carotid arteries in 40-70 years age group females 
Scientific Title of Study   Association of vascular calcification of breast with carotid intima medial thickness in perimenopausal women 
Trial Acronym   
Secondary IDs if Any  
Secondary ID  Identifier 
NIL  NIL 
 
Details of Principal Investigator or overall Trial Coordinator (multi-center study)  
Name  N PRAJEETH RAO 
Designation  JUNIOR RESIDENT 
Affiliation  KASTURBA MEDICAL COLLEGE 
Address  room no 9, near sai baba statue, shiridi sai cancer block Department of Radiodiagnosis and Imaging, Kasturba Medical College, Madhav Nagar, Near Tiger Circle, Manipal, Udupi District

Udupi
KARNATAKA
576104
India 
Phone  9566751279  
Fax    
Email  prajithng@gmail.com  
 
Details of Contact Person
Scientific Query
 
Name  SMITI SRIPATI 
Designation  PROFESSOR 
Affiliation  KASTURBA MEDICAL COLLEGE 
Address  Department of Radiodiagnosis and Imaging, Kasturba Medical College, Madhav Nagar, Near Tiger Circle, Manipal, Udupi District

Udupi
KARNATAKA
576104
India 
Phone  9449640418  
Fax    
Email  smitis11@hotmail.com  
 
Details of Contact Person
Public Query
 
Name  Dr PRIYA P S 
Designation  ASSISTANT PROFESSOR 
Affiliation  KASTURBA MEDICAL COLLEGE 
Address  Department of Radiodiagnosis and Imaging, Kasturba Medical College, Madhav Nagar, Near Tiger Circle, Manipal, Udupi District

Udupi
KARNATAKA
576104
India 
Phone  9895563264  
Fax    
Email  priya.psankaran@gmail.com  
 
Source of Monetary or Material Support  
Department of Radiodiagnosis Near new trauma block Kasturba hospital Manipal 
 
Primary Sponsor  
Name  kasturba medical college and hospital 
Address  USG room, near shirdi sai statue, shirdi sai cancer block, kasturba hospital, madhava nagar, manipal- 576104  
Type of Sponsor  Private medical college 
 
Details of Secondary Sponsor  
Name  Address 
NIL  NIL 
 
Countries of Recruitment     India  
Sites of Study  
No of Sites = 1  
Name of Principal Investigator  Name of Site  Site Address  Phone/Fax/Email 
N PRAJEETH RAO  Shiridi Sai cancer block, kasturba hospital  room number 9, shiridi ultrasound room, near sai baba statue department of radiodiagnosis and imaging shiridi sai cancer block kasturba hospital manipal
Udupi
KARNATAKA 
9566751279

prajithng@gmail.com 
 
Details of Ethics Committee  
No of Ethics Committees= 1  
Name of Committee  Approval Status 
institutional ethics committee  Approved 
 
Regulatory Clearance Status from DCGI  
Status 
Not Applicable 
 
Health Condition / Problems Studied  
Health Type  Condition 
Healthy Human Volunteers  vascular calcification in breast 
 
Intervention / Comparator Agent  
Type  Name  Details 
 
Inclusion Criteria  
Age From  40.00 Year(s)
Age To  70.00 Year(s)
Gender  Female 
Details  Cases are the perimenopausal age group women with radiographically detected vascular calcifications in the breast.
Controls are the perimenopausal age group women without breast vascular calcifications.
Both the groups will be undergoing the Carotid doppler. 
 
ExclusionCriteria 
Details  Patients who have vascular calcifications after 70 years of age. 
 
Method of Generating Random Sequence   Not Applicable 
Method of Concealment   Not Applicable 
Blinding/Masking   Not Applicable 
Primary Outcome  
Outcome  TimePoints 
to assess the relation between the breast vascular calcification with carotid intima medial thickness  time point is 0, as the carotid intima medial thickness was assessed only after subjects underwent screening mammogram  
 
Secondary Outcome  
Outcome  TimePoints 
To assess strength of association between the breast vascular calcification and carotid intima medial thickness considering other clinical risk factors for intimal calicfication  time point is 0 
 
Target Sample Size   Total Sample Size="100"
Sample Size from India="100" 
Final Enrollment numbers achieved (Total)= "Applicable only for Completed/Terminated trials"
Final Enrollment numbers achieved (India)="Applicable only for Completed/Terminated trials" 
Phase of Trial   N/A 
Date of First Enrollment (India)   12/09/2017 
Date of Study Completion (India) Applicable only for Completed/Terminated trials 
Date of First Enrollment (Global)  Date Missing 
Date of Study Completion (Global) Applicable only for Completed/Terminated trials 
Estimated Duration of Trial   Years="0"
Months="11"
Days="19" 
Recruitment Status of Trial (Global)   Not Applicable 
Recruitment Status of Trial (India)  Open to Recruitment 
Publication Details   none yet 
Individual Participant Data (IPD) Sharing Statement

Will individual participant data (IPD) be shared publicly (including data dictionaries)?  

Brief Summary  
1. TITLE: Association of vascular calcification of breast with carotid intima media thickness in
peri- menopausal women.
2. INTRODUCTION: Breast arterial calcification (BAC) is a common finding on
mammographies, and is identified as medial calcific sclerosis of small to medium-
sized muscular arteries of breast. On mammography, BAC typically appears as
linear, parallel lines resembling a railroad or as ring-like calcifications in cross
section. Previous reports suggest that the presence of Breast arterial calcification
on routine screening mammography is associated with the presence of
hypertension,diabetes mellitus,angiographically defined coronary artery disease
(CAD) and increased cardiovascular mortality.
Carotid intima-media thickness (C-IMT) is an easily and quickly measured
noninvasive ultrasonographic marker of early atherosclerotic disease beyond the
classic cardiovascular risk factors, and is directly associated with future clinical
events such as myocardial infarction or stroke and cardiovascular mortality.Even
though Breast arterial calcification was shown to be related with coronary artery
disease and hypertension and diabetes mellitus, the association between Breast
arterial calcification and Carotid intima-media thickness has not been evaluated
as yet. Therefore, this study was designed to test the hypothesis that Breast
arterial calcification on mammography might be associated with Carotid intima-
media thickness.
3)TYPE OF STUDY: Cross sectional Study.
4) AIMS AND OBJECTIVES:
AIM: To determine the relationship between vascular calcification in breast
with carotid intimal thickness in the perimenopausal women
OBJECTIVES:
1) To evaluate the vascular calcification in breast and correlate with carotid
intimal thickness
2) To study breast vascular calcification as a predictor for cardiovascular
morbidity and mortality
5)JUSTIFICATION FOR STUDY:

Mammography is a recommended screening investigation of breast for
women above 40-70 years of age for early detection of the breast cancer.in this
technique we use low dose x ray radiation for detection of the breast lesions.
Arterial calcification is a common feature of atherosclerosis which can be elicited
with conventional radiological imaging as calcium deposits in the arterial wall. The
appearance of calcium in different vascular beds occurs 10-15 years later in
women than in men. Breast Arterial Calcification (BAC) is an incidental finding in
the mammogram which can be used to correlate the risk factors for
cardiovascular morbidity and mortality by calculating the carotid intima medial
thickness.
6) DEPARTMENTS INVOLVED:
RADIODIAGNOSIS.

7) STUDY PERIOD: From september 2017 to September 2018

8) SAMPLE SIZE : 100
9) MATERIALS AND METHODS:
This prospective study will be conducted in department of RadioDiagnosis
and imaging, KMC, Manipal from September 2016 to August 2018
Institutional ethics committee approval will be obtained and informed consent
will be taken from the patient prior to the procedure.
A) EQUIPMENT:
B) INCLUSION CRITERIA:
1) Cases are the perimenopausal age group women with radiographically
detected vascular calcifications in the breast.
2) Controls are the perimenopausal age group women without breast
vascular calcifications.
3) Both the groups will be undergoing the Carotid doppler.
EXCLUSION CRITERIA:
1) Patients who have vascular calcifications after 70 years of age.


C) BIOLOGICAL MATERIALS REQUIRED-NIL

D) STATISTICAL ANALYSIS:Statistical analyses will be carried out on SPSS
platform and the significance of the grade of vascular calcification in breast
with the carotid intima medial thickness will be assessed in consideration
with the other factors like the age of menopause, h/o hypertension,
hyperlipidemia and diabetes mellitus and significance will be assessed using
the P value.

10) DETAILED DESCRIPTION OF THE PROCEDURE: Subjects for the study are
the Perimenopausal age group women who are divided into two groups cases
and controls, cases are the women who are found to have vascular
calcifications in breast. Control groups are perimenopausal women without
breast arterial calcifications. Breast arterial calcifications (BAC) was defined as
two linear calcification depositions in a conical periphery when the arterial
wall is imaged longitudinally or as calcific rings when the artery is cut
transversely.grading for the calcification will be done according to:
Grade 1- arteries faintly outlined by calcium
Grade 2- predominantly outlined by calcium
Grade 3- markedly outlined by calcium.
Carotid intima thickness will be measured at 1 cm proximal to the carotid bulb
and 3 measurements will be taken at 1mm interval in both carotid arteries and
average of these will be taken. Abnormal intimal medial thickness is considered if
thickness is more than 0.9 mm.
Preliminary history will be take about an ingestion of the lipid lowering
agents, previous h/o diabetes, h/o any medications taken in the past.
Patients with breast arterial calcification will be graded into two categories both
cases and control groups will undergo carotid intima media thickness study from
the both common carotid arteries and these values will be taken from the
proximal, mid and distal common carotid artery with the above said statistical
analysis.

11) OUTCOME MEASURES: Cases with breast arterial calcification will be
graded into two categories and the average of the carotid intimal thickness will
be taken based on the thickness and calcification in the carotid the relative
risk of vascular calcification in breast with carotid intima medial thickness is
assessed.
12) POTENTIAL RISKS AND BENEFITS: There is no direct benefit to the
participant.
13) ETHICAL CONSIDERATIONS AND METHODS TO ADDRESS ISSUES: The
project will be reviewed and approved before recruitment of participants.
Individual informed consent will be obtained before enrollment of participant.
14) BUDGET AND PROPOSED FUNDING: NIL
15) REVIEW OF LITERATURE:
In a study conducted by Sema Yildiz, Ali Yildiz, Nesrin Ertug et al concluded that
the BAC on mammography is independently associated with C-IMT. Screening
mammographies merit to be evaluated for the presence of BAC, which might
benefit cardiovascular preventive medicine in women by predicting
atherosclerosis [1].
In a study conducted by Besir erdogmus et al concluded that significant
relationship exists between Brachial intimal medial thickness and breast arterial
calcification but if age matching was done there will be no significant relationship
between vascular calcification in breast and carotid intimal thickness [2].
In a study conducted by Ramazan Buyukkaya, Ayla Buyukkaya, Aysegul Altunkas
et al concluded that BAC was found to be significantly and independently
associated with C-IMT [3].
In a study conducted by Neeraj Shah, Vinod Chainani, Patrice Delafontaine et al
concluded that BAC is associated with CVD, stroke, CIMT and CAC, however BAC’s
relation with angiographic CAD is uncertain and presently debatable [4].

16) REFERENCES
[1] Besir Erdogmus, Ramazan Buyukkaya, Ayla Buyukkaya, Burhan Yazıcı et al
published an article titled “Relationship between breast arterial calcifications
detected on mammography and brachial artery intima-media thickness” in Wien
Klin Wochenschr (2013) 125:100–104.
[2] Sema Yildiz, Ali Yildiz, Nesrin Ertug et al published an article titled “Association
of breast arterial calcification and carotid intima-media thickness” in Springer
Heart Vessels (2008) 23:376–382
[3] Ramazan Buyukkaya, Ayla Buyukkaya, Aysegul Altunkas et al published an
article titled “Breast arterial calcifications and carotid intima-media thickness and
haemodynamics: Is there any association?” in Turkish society of cardiology on
2014; 14: 378-82
[4] Neeraj Shah, Vinod Chainani, Patrice Delafontaine et al published an article
titled “Mammographically Detectable Breast Arterial Calcification and
Atherosclerosis” in Cardiol Rev. 2014 ; 22(2): 69–78.
doi:10.1097/CRD.0b013e318295e029.
 
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