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CTRI Number  CTRI/2018/06/014381 [Registered on: 01/06/2018] Trial Registered Prospectively
Last Modified On: 28/06/2018
Post Graduate Thesis  No 
Type of Trial  BA/BE 
Type of Study   NA 
Study Design  Randomized, Crossover Trial 
Public Title of Study   Bioequivalence study of Imatinib Mesylate Tablets 400mg in patients with chronic myeloid leukemia or gastrointestinal stromal tumors 
Scientific Title of Study   A multi-center, open-label, balanced, randomized, two-treatment, two-period, two-sequence, two-way crossover, steady-state bioequivalence study of Imatinib Mesylate Tablets 400mg of Hetero Labs Limited, India with GLEEVEC® (Imatinib Mesylate) Tablets 400mg of Novartis Pharmaceuticals Corporation, NJ 07936 among patients with chronic myeloid leukemia or gastrointestinal stromal tumors established on a stable dose of 400mg for Imatinib Mesylate Tablets  
Trial Acronym   
Secondary IDs if Any  
Secondary ID  Identifier 
BE/18/216 version 00 dated 3-May-2018  Protocol Number 
F.No. TL/BE/18/001312  DCGI 
 
Details of Principal Investigator or overall Trial Coordinator (multi-center study)  
Name  Dr Amrut Jadhav 
Designation  Medical Monitor 
Affiliation  Raptim Research Ltd. 
Address  Raptim Research Ltd. Clinical Pharmacology Unit (A-226), Bioanalytical and Biostatistical Unit (A-242) T.T.C., Industrial Area, Mahape M.I.D.C., Navi Mumbai. Thane MAHARASHTRA India

Thane
MAHARASHTRA
400701
India 
Phone  02227781889  
Fax  02227781884  
Email  amrut.jadhav@raptimresearch.com  
 
Details of Contact Person
Scientific Query
 
Name  Dr Amrut Jadhav 
Designation  Medical Monitor 
Affiliation  Raptim Research Ltd. 
Address  Raptim Research Ltd. Clinical Pharmacology Unit (A-226), Bioanalytical and Biostatistical Unit (A-242) T.T.C., Industrial Area, Mahape M.I.D.C., Navi Mumbai. Thane MAHARASHTRA India

Thane
MAHARASHTRA
400701
India 
Phone  02227781889  
Fax  02227781884  
Email  amrut.jadhav@raptimresearch.com  
 
Details of Contact Person
Public Query
 
Name  Mustafa Pardiwala 
Designation  Senior Project Manager 
Affiliation  Raptim Research Ltd. 
Address  Raptim Research Ltd. Clinical Pharmacology Unit (A-226), Bioanalytical and Biostatistical Unit (A-242) T.T.C., Industrial Area, Mahape M.I.D.C., Navi Mumbai. Thane MAHARASHTRA India

Thane
MAHARASHTRA
400701
India 
Phone  02227781889  
Fax  02227781884  
Email  mustafa.pardiwala@raptimresearch.com  
 
Source of Monetary or Material Support  
Hetero Labs Limited 7-2-A2, Hetero Corporate, Industrial Estates, Sanathnagar, Hyderabad – 500 018, Telangana, India 
 
Primary Sponsor  
Name  Hetero Labs Limited 
Address  7-2-A2, Hetero Corporate, Industrial Estates, Sanathnagar, Hyderabad – 500 018, Telangana, India 
Type of Sponsor  Pharmaceutical industry-Indian 
 
Details of Secondary Sponsor  
Name  Address 
NIL  NIL 
 
Countries of Recruitment     India  
Sites of Study
Modification(s)  
No of Sites = 5  
Name of Principal Investigator  Name of Site  Site Address  Phone/Fax/Email 
Dr M Gopichand  HCG City Cancer Centre  HCG City Cancer Centre, Department of Oncology, Ground Floor, R.No-35-25-33, Ch. venkata Krishnaya Street, Suryarao Pet, Vijayawada-5200021, A.P, India
Krishna
ANDHRA PRADESH 
919885256059

mgopichand@yahoo.com 
Dr Rajnish Nagarkar  HCG Manavata Cancer Centre  HCG Manavata Cancer Centre OPD, Department of Oncology, Ground Floor, Opp. Mahamarg Bus Stand Mumbai Naka Nashik 422004
Nashik
MAHARASHTRA 
919822574157

drraj@cmccnashik.in 
Dr Niraj Bhatt  Kailash Cancer Hospital & Research Centre  Kailash Cancer Hospital & Research Centre, Department of Oncology, Muniseva Ashram, Goraj-391760
Vadodara
GUJARAT 
919925581480

niraj.bhatt@greenashram.org 
Dr Minish Jain  Noble Hospital  Department of Oncology, Ground Floor,153-Magarpatta City Road,
Pune
MAHARASHTRA 
9823133390

minishjain009@gmail.com 
Dr Ankit Patel  Unique Hospital Multispeciality & Research Institute  Department of Oncology, Ground Floor, Opp Kiran Motors Near Canal Civil Hospital Char Rasta Sosyo Circle Lane Off Ring Road Surat
Surat
GUJARAT 
919825404202

drankitoncologist@gmail.com 
 
Details of Ethics Committee
Modification(s)  
No of Ethics Committees= 5  
Name of Committee  Approval Status 
Ethics Committee Unique Hospital Multispeciality & Research Institute  Approved 
Institute Ethics Committee City Cancer Centre   Approved 
Kailash Cancer & Medical Centre Institutional Ethics Committee  Approved 
Manavata Clinical Research Institute Ethics Committee  Approved 
Noble Hospital Institutional Ethics Committee  Approved 
 
Regulatory Clearance Status from DCGI
Modification(s)  
Status 
Approved/Obtained 
 
Health Condition / Problems Studied  
Health Type  Condition 
Patients  Chronic Myeloid Leukemia and Gastrointestinal Stromal Tumors,  
 
Intervention / Comparator Agent  
Type  Name  Details 
Comparator Agent  GLEEVEC® (Imatinib Mesylate) Tablets 400mg of Novartis Pharmaceuticals Corporation East Hanover, New Jersey 07936.  As per randomization schedule, subjects will be instructed to receive Test or Reference products according of 400mg Imatinib once a day for entire study duration of 14 days. 
Intervention  Imatinib Mesylate Tablets 400mg of Hetero Labs Limited, India.  As per randomization schedule, subjects will be instructed to receive Test or Reference products according of 400mg Imatinib once a day for entire study duration of 14 days. 
 
Inclusion Criteria  
Age From  18.00 Year(s)
Age To  65.00 Year(s)
Gender  Both 
Details  A patient fulfilling all the following criteria will be included in the present study:
1) Patients
Willing to provide written informed consent for participation in the study;
Having ability to comprehend the nature and purpose of the study;
Willing to be available for the entire study period and to comply with protocol requirements.
2) Disease population and treatment condition –
Patients with documented clinical diagnosis of Philadelphia chromosome positive (Ph+) chronic myeloid leukemia (CML) in chronic phase under their first three months of treatment at stabilized 400mg dose of Imatinib Tablets once daily;

OR
Patients with documented clinical diagnosis of Kit (CD117) positive gastrointestinal stromal tumors (GIST) at stabilized 400mg dose of Imatinib Tablets once daily.
3) Patients for whom a titration away from the stabilized once daily dose of 400mg of Imatinib is unlikely for a study duration;
4) Age of 18-65 years (both inclusive);
5) Patients with Eastern Cooperative Oncology Group (ECOG) performance status of 0 – 2 (both inclusive);
6) Patients with life expectancy of at least 3 months;
7) Adequate organ function, defined as following:
a)Hemoglobin > 9.0 g/dL
b)Absolute neutrophil count (ANC) ≥ 1500 cells/mm3
c)Platelet count ≥ 100,000/ mm3
d)Total bilirubin <1.5 x Upper Limit of Normal (ULN)
e)SGPT / SGOT < 2.5 x ULN
f)Serum Creatinine < 1.5 x ULN
8) With a normal or clinically non-significant laboratory values (apart from those mentioned above) as determined by hematological, biochemistry tests and urine analysis;
9) With a normal or clinically non-significant 12-lead ECG;
10) With a normal or clinically non-significant 2D Echo;
11) Non-smokers or ex-smoker or mild / moderate smokers and willing to abstain from chewing or smoking any tobacco containing product at least 72.00 hrs prior to first dosing of the study and throughout study;
12) Willing to abstain from alcohol or alcoholic products within 24.00 hrs prior to first dosing of the study and throughout the study;
13) Willing to abstain from, xanthine or its derivative containing food or beverages (e.g. chocolates, tea, coffee or cola drinks) within 72.00 hrs prior to first dosing of the study and throughout the study;
14) Willing to abstain from grapefruit or its juice within 72.00 hrs prior to first dosing of the study and throughout the study;
15) In case of female patients:
a) Negative urine pregnancy test during screening and, negative serum β-HCG test at baseline visits and at check-in for housing during each study period;
b) Patients with child bearing potential or those within their first two years of onset of menopausal syndrome must either abstain from sexual intercourse, or must be using at least 2 reliable and acceptable methods of birth control till 14 days after last dose in the study (Reliable/acceptable birth control methods include barrier methods such as diaphragm/condom with spermicide or who are surgically sterile (bilateral tubal ligation, bilateral salpingectomy, bilateral oophorectomy or hysterectomy has been performed), but must not use hormonal contraceptive (either oral/implants)).
 
 
ExclusionCriteria 
Details  A patient fulfilling any one of the following criteria will be excluded from the study:
1) Institutionalized patients;
2) A history of allergic or hypersensitive reactions to Imatinib Mesylate or related group of drugs or to any of the excipients of formulation which in the opinion of an investigator, would compromise the safety of the patient or the study;
3) Patients with Ph+ CML in accelerated phase or blast crisis phase;
4) Patients receiving any treatment for CML prior to study entry for longer than 2 weeks with the exception of Hydroxyurea and/or Anagrelide and/or Imatinib;
5) History of another primary malignancy except if the other primary malignancy is neither currently clinically significant or requiring active intervention;
6) Significant history or current evidence of chronic – infections, cardiovascular, renal, hepatic, ophthalmic, pulmonary, neurological, metabolic (endocrine), hematological, gastrointestinal, immunological or psychiatric diseases, or organ dysfunction, which in the opinion of an investigator, would compromise the safety of the patient or the study;
7) History of heart failure, renal insufficiency, hypereosinophilic syndrome (HES), myelodysplastic syndrome (MDS)/ myeloproliferative disease (MPD) or acute systemic mastocytosis (ASM)
8) Patients who are at clinically high risk of developing tumor lysis syndrome during the study;
9) Patients with history of bullous dermatologic reactions (e.g., erythema multiforme and Stevens-Johnson syndrome) with Imatinib or any other drug treatment;
10) Patients with a history of ascites, and rapid weight gain with or without superficial edema;
11) Previous/current history of hematopoietic stem cell transplantation;
12) History or currently under significant bleeding disorder unrelated to CML and/or receiving concomitant therapy of Warfarin (coumarin anticoagulants) or history of usage of coumarin anticoagulants in the previous three months prior to study start (dosing);
13) History of difficulty in swallowing which could affect drug absorption;
14) A medical or surgical condition that might interfere with the absorption, metabolism, or excretion of Imatinib Mesylate during the study;
15) History of significant hematological disorder (drug-induced or idiopathic);
16) History of significant liver disease;
17) History or requirement of therapy with CYP3A4 activity inducer or inhibitor drug within 14 days prior to first dosing of the study and till study completion;
18) Concurrent use of other drugs known to suppress bone marrow function;
19) Patients who have undergone thyroidectomy and/or receiving Levothyroxine
20) Expected significant changes in doses of concomitant medications during the study
21) Expected incompliance with outpatient medications;
22) A history of alcohol or drug dependence during the 6-month period immediately prior to first dosing of the study;
23) Positive alcohol breath or urine drug of abuse tests during randomization, or at check-in for housing during each study period;
24) Positive test for Human Immunodeficiency Virus (HIV) type I/II antibodies or Hepatitis B surface antigen (HbsAg) or Hepatitis C virus (HCV) antibodies;
25) In case of female patients:
a) Planning to become pregnant;
b) Lactating or nursing patients;
26) Participated in any clinical investigation requiring repeated blood sampling, blood donation, or have blood loss of >200 mL in past 8 weeks or participated in any clinical study within 90 days prior to first dosing of the study;
27) Any major illness or hospitalization within 90 days prior to first dosing of the study;
28) History of difficulty in accessibility of veins in arms.
 
 
Method of Generating Random Sequence   Computer generated randomization 
Method of Concealment   Centralized 
Blinding/Masking   Open Label 
Primary Outcome  
Outcome  TimePoints 
The primary objective of the study is to assess whether the test product is bioequivalent to reference product after dosing subjects for 14 days on 400mg once a day Imatinib  The primary objective of the study is to assess whether the test product is bioequivalent to reference product after dosing subjects for 14 days on 400mg once a day Imatinib 
 
Secondary Outcome  
Outcome  TimePoints 
The secondary objective is assessment of safety and tolerability profile of test and reference products.  During each study period, pre-dose samples will be collected on days - 5, 6, 12 and 13 for morning dose. Post-dose PK samples will be collected on day-7 and 14 of each study period at 0.50, 1.00, 1.50, 2.00, 2.50, 3.00, 3.50, 4.00, 4.50, 5.00, 6.00, 8.00, 10.00, 12.00, 16.00 and 24.00 hours post dose 
 
Target Sample Size   Total Sample Size="34"
Sample Size from India="34" 
Final Enrollment numbers achieved (Total)= "Applicable only for Completed/Terminated trials"
Final Enrollment numbers achieved (India)="Applicable only for Completed/Terminated trials" 
Phase of Trial   N/A 
Date of First Enrollment (India)   08/06/2018 
Date of Study Completion (India) Applicable only for Completed/Terminated trials 
Date of First Enrollment (Global)  Date Missing 
Date of Study Completion (Global) Applicable only for Completed/Terminated trials 
Estimated Duration of Trial   Years="0"
Months="5"
Days="0" 
Recruitment Status of Trial (Global)   Not Applicable 
Recruitment Status of Trial (India)  Not Yet Recruiting 
Publication Details   None yet 
Individual Participant Data (IPD) Sharing Statement

Will individual participant data (IPD) be shared publicly (including data dictionaries)?  

Brief Summary   Imatinib Mesylate is a protein-tyrosine kinase inhibitor that inhibits the BCR-ABL tyrosine kinase, the constitutive abnormal tyrosine kinase created by the Philadelphia chromosome abnormality in Chronic Myeloid Leukemia (CML). Imatinib is also an inhibitor of the receptor tyrosine kinases for platelet-derived growth factor (PDGF) and stem cell factor (SCF), c-kit, and inhibits PDGF-and SCF-mediated cellular events. In vitro, Imatinib inhibits proliferation and induces apoptosis in Gastrointestinal Stromal Tumor (GIST) cells, which express an activating c-kit mutation.

Present trial is a 
multi-center, open-label, balanced, randomized, two-treatment, two-period, two-sequence, two-way crossover, steady-state bioequivalence study of Imatinib Mesylate Tablets 400mg of Hetero Labs Limited, India with GLEEVEC® (Imatinib Mesylate) Tablets 400mg of Novartis Pharmaceuticals Corporation, NJ 07936 among patients with chronic myeloid leukemia or gastrointestinal stromal tumors established on a stable dose of 400mg for Imatinib Mesylate Tablets under fed condition.

The present study includes two periods – each of 7 days (period-I: days 1-7; period-II: days 8-15) with no wash-out period between them. As per randomization schedule, subjects will be instructed to receive test or reference products of stabilized dose of Imatanib 400 mg tablets per day  in each of the study period.

Pre-dose PK samples will be collected on days -5, 6, 12 and 13 during each study period. Post-dose PK samples will be collected on day-7 and 14 of each study period at 0.50, 1.00, 1.50, 2.00, 2.50, 3.00, 3.50, 4.00, 4.50, 5.00, 6.00, 8.00, 10.00, 12.00, 16.00 and 24.00 hours post dose.

Plasma samples will be analyzed for Imatinib using a validated analytical method in accordance with USFDA Guidelines and in-house SOPs of bioanalytical laboratory. Safety of the subjects will be monitored during the entire study period. Test product will be considered bioequivalent to reference product, if 90% CI for ratio of geometric least square means based on log transformed primary PK parameters - Cmax,ss and AUC0-Ï„,ss fall within acceptable BE limits of 80.00% to 125.00% for Imatinib.

 
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