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CTRI Number  CTRI/2011/04/001657 [Registered on: 01/04/2011] Trial Registered Prospectively
Last Modified On: 16/06/2011
Post Graduate Thesis  No 
Type of Trial  Interventional 
Type of Study   Drug
Biological 
Study Design  Randomized, Parallel Group, Multiple Arm Trial 
Public Title of Study   A clinical trial to compare different administrations of the investigational drug Plasmin in patients with a clot in an artery of the lower leg. 
Scientific Title of Study   T05018-2004: A Phase 2, Randomized, Open-label (with Blinded Plasminogen Activator and Placebo Control Groups) Study to Evaluate the Effects of Different Intra- thrombus Infusion Regimens of Plasmin (Human) Compared to Plasminogen Activator and Placebo In Patients With Acute Lower Extremity Native Artery or Bypass Grafi Occlusion 
Trial Acronym   
Secondary IDs if Any  
Secondary ID  Identifier 
NCT01222117  ClinicalTrials.gov 
TO5018-2004  Protocol Number 
 
Details of Principal Investigator or overall Trial Coordinator (multi-center study)  
Name  Ataali shaikh 
Designation  General Manager 
Affiliation  Kendle India 
Address  General Manager
Kendle India Pvt Ltd. Unit 002, Ground Floor, Tower C, Cyber Park, Sector 39,
Gurgaon
HARYANA
122001
India 
Phone  911244536357  
Fax  911244536301  
Email  shaikh.ataali@kendle.com  
 
Details of Contact Person
Scientific Query
 
Name  Vishal Patel 
Designation  Senior Project Leader 
Affiliation  Kendle India 
Address  Senior Project Leader
Global Project Management Kendle, B-901, Safal Pegasus, 100 Feet Road, Near Auda Garden, Prahladnagar, Anand nagar Road. Ahmedabad - 380 051 Gujarat, INDIA.
Ahmadabad
GUJARAT
380 051
India 
Phone  917940213610  
Fax  917940213601  
Email  patel.vishal@kendle.com  
 
Details of Contact Person
Public Query
 
Name  Vivek Malhotra 
Designation  Regulatory Manager 
Affiliation  Kendle India 
Address  Regulatory Manager
Kendle India Pvt Ltd. Unit 002, Ground Floor, Tower C, Cyber Park, Sector 39,
Gurgaon
HARYANA
122001
India 
Phone  911244536357  
Fax  911244536301  
Email  malhotra.vivek@kendle.com  
 
Source of Monetary or Material Support  
Talecris Biotherapeutics, Inc.4 10 1 Research Commons, 79 T. W. Alexander Drive, Research Triangle Park, North Carolina 27709 USA 
 
Primary Sponsor  
Name  Talecris Biotherapeutics Inc  
Address  4101 Research Commons, 79 T. W. Alexander Drive, Research Triangle Park, North Carolina 27709 USA 
Type of Sponsor  Pharmaceutical industry-Global 
 
Details of Secondary Sponsor  
Name  Address 
Kendle India Private Limited  Kendle India Pvt Ltd. Unit 002, Ground Floor, Tower C, Cyber Park, Sector 39, Gurgaon 122001 Haryana, India Tel 911244536 300 
 
Countries of Recruitment     India
Sri Lanka
Belgium
Brazil
Bulgaria
Czech Republic
Germany
Peru
Poland
Romania
Russian Federation
Serbia
Slovakia
Spain
Ukraine
United States of America  
Sites of Study  
No of Sites = 16  
Name of Principal Investigator  Name of Site  Site Address  Phone/Fax/Email 
Dr Kamal Sharma  Apollo Hospitals International Limited, Department of Cardiology,   Clinical Research Department Second Floor Plot No. 1 A, Bhat GIDC Estate, Gandhinagar-382428, Gujarat, India.
Ahmadabad
GUJARAT 
09925195662

kamalsharma1975@rediffmail.com 
Dr Tarun Gandhi  CHL Apollo Hospitals, Department of Cardio thoracic and Vascular Surgery.  Clinical Research Department Second Floor A.B. Road, Near L.I.G. Triangle, Indore-452008, Madhya Pradesh, India 452008
Indore
MADHYA PRADESH 
9009090203

doc_tgandhi@yahoo.co.in 
Dr S Sudindran  Department of Vascular surgery, Amrita Institute of medical science & Research,   1st Floor, Tower 1, Kochi, Kerala, 682041
Thiruvananthapuram
KERALA 
9995022194

sudhi@aims.amrita.edu 
Dr Hemanth Pandharpurkar  Dept. of Surgery, St. Johns Medical College Hospital   2nd Floor, Sarjapur Road, Bangalore, Karnataka, 560034
Bangalore
KARNATAKA 
09811047390

dr_hemanth1@rediffmail.com 
Dr R K Pinjala  Dept. Vascular Surgery, NIMS,   Punjagutta, Hyderabad, Andhra Pradesh-500082, India
Hyderabad
ANDHRA PRADESH 
09848050243

pinjala@hotmail.com 
Dr Rakesh Mahajan   Educational and Reseach Foundation, Indraprastha Apollo Hospitals,   Sarita Vihar, New Delhi - 110044
New Delhi
DELHI 
09910881463

mahajanr10@hotmail.com 
Dr Sameer Dani  Life Care Institute of Medical Sciences and Research, Department of Cardiology   Clinical Research Department 3rd Floor, Near Sardar Patel statue, Naranpura, cross road, Ahmedabad,Gujarat,,-380014
Ahmadabad
GUJARAT 
09825038855

danisameer@hotmail.com 
Dr Sanjay Desai  M.S. Ramaiah Memorial Hospitals, Dept of Vascular and Endovascular Surgery  Ground Floor, MSRIT Post, New BEL Road, Bangalore, Karnataka, 560054
Bangalore
KARNATAKA 
09886880711

scdesai@hotmail.com 
Dr Kumud Rai   Max Super speciality Hospital, Department of Vascular Surgery  2 Press Enclave Road, Saket, New Delhi
New Delhi
DELHI 
09810205525

kumud.rai@maxhealthcare.com 
Dr Rajiv Parakh  Medanta The Medicity, Department Pheripheral Vascular and Endo-vascular Sciences   Sector - 38, Gurgaon 122001
Gurgaon
HARYANA 
09811047390

Rajiv.Parakh@medanta.org 
Dr Mukund Kumble  Omega Hospital. Department of Cardiology  4th Floor, Mahaveer Circle , Kankanadi, Mangalore, Karnataka, 575002, India.
Bangalore
KARNATAKA 
9845082811

doctormukund@heartcarehealth.com 
Dr Dhanesh Kamerkar  Ruby Hall Clinic, Department of Vascular and Endo vascular Surgery  Gound Floor, 40, Sassoon Road, Pune - 411001, India.
Pune
MAHARASHTRA 
012066455140

dhaneshkamerkar@gmail.com 
Dr Sunil Thanvi  Shalby Hospitals, Clinical Research Unit, 9th floor  Opp Karnavati Club S.G.Road,Ahmedabad Gujarat (INDIA), 380015
Ahmadabad
GUJARAT 
917940203115

sunil.thanvi@shalby.org 
Dr Ashwani Mehta  Sir Ganga Ram Hospital, Dharam Vira heart Center  5th Floor, Old Rajinder Nagar, New delhi - 110060
New Delhi
DELHI 
09811057384

drashwanimehta@gmail.com 
Dr Arun Gupta  Sree Chitra Tirunal Inst for Medical Sciences and Tech, Deptt of Imaging & Interventional Radiology  Ground Floor, Trivandrum, Kerala, 695011, India
Thiruvananthapuram
KERALA 
09447900144

gupta209@gmail.com 
Dr Shrenik Shah  Sterling Hospital, R&D department, R and D department, Eight Floor   Sterling Hospitals Road, Memnagar, Ahmedabad-380052, Gujarat, India
Ahmadabad
GUJARAT 
09825015959

dr_shrenik@yahoo.co.in 
 
Details of Ethics Committee
Modification(s)  
No of Ethics Committees= 16  
Name of Committee  Approval Status 
Amrita institute of Medical sciences Dr. Sudhindran Institutional Ethics Committee, Amrita Institute of Medical Sciences and research Center   Submittted/Under Review 
Apollo Hospitals International Limited Dr. Kamal Sharma Ethics Committee - Apollo Hospitals International Limited   Approved 
CHL-Apollo Hospital Dr. Tarun Gandhi CHL ? Apollo Hospitals - Ethics Committee   Approved 
Hospital Ethics Committee, Life care Institute of Medical science and Research, Dr. Sameer Dani   Approved 
Indraprastha Appolo Hospital Dr. Rakesh mahajan Ethics Committee on Clinical Trials, Indraprastha Apollo Hospitals   Submittted/Under Review 
M.S. Ramaiah Memorial Hospital Dr. Sanjay Desai M.S. Ramaiah Medical College and Teaching Hospital, Ethical review Board.   Approved 
Max Devki devi Dr. Kumud Rai Institutional Review Board   Submittted/Under Review 
Medanta, The medicity Dr. Rajiv Parakh Medanta Instituional Review Board and Medanta Institutinal ethics committee.   Submittted/Under Review 
Nizam Institute of Medical sciences Dr. R.K. Pinjala Institutional Ethics Committee, Nizam?s Institute of Medical Sciences   Approved 
Omega Hospital Dr. Mukunda Kumbla Canara Research Ethical Committee   Approved 
Ruby Hal Clinic Dr. Dhanesh Kamerkar Ethics Committee-Poona Medical Research Foundation,   Approved 
Shalby Hospital Dr. Sunil Thanvi Ethics Committee Shalby Hospitals  Approved 
Sir Ganga Ram Hospital Dr. Ashwani Mehta Ethics Committee Sir Gangaram Hospital,   Submittted/Under Review 
Sree Chitra Tirunal Institute for Medical Sciences & Technology Dr. Arun Gupta Ethics Committee, Sree Chitra Tirunal Institute for Medical Sciences & Technology   Submittted/Under Review 
St. John Medical college Dr. Hemanth Pandharpurkar Ethical Review Board   Approved 
Sterling Hospital Dr. Shrenik Shah Ethics Committee   Approved 
 
Regulatory Clearance Status from DCGI  
Status 
Approved/Obtained 
 
Health Condition / Problems Studied  
Health Type  Condition 
Patients  Patients With Acute Lower Extremity Native Artery or Bypass Grafi Occlusion,  
 
Intervention / Comparator Agent  
Type  Name  Details 
Comparator Agent  Placebo (0.9% NaCl for injection)  Volume to match the equivalent PA volume according to investigator clinical judgment. 
Intervention  Plasmin (Human)  150 mg Plasmin - Distal Pulse and Infusion 
Intervention  Plasmin (Human)  150 mg Plasmin - Proximal Pulse and 2-Hr High Infusion Rate  
Intervention  Plasmin (Human)  150 mg Plasmin - Proximal Pulse and High Infusion Rate 
Intervention  Plasmin (Human)  150 mg Plasmin - Proximal Pulse and Infusion 
Comparator Agent  Plasminogen Activater  PA Dose And Volume According To The Investigators Clinical Judgment 
 
Inclusion Criteria  
Age From  18.00 Year(s)
Age To  99.00 Year(s)
Gender  Both 
Details  Inclusion Criteria
1. Unilateral limb ischemia: symptomatic, SVS acute ischemic Categories I and IIa
2. Onset of symptoms less than or equal to 14 days
3. Thrombosed (non-embolic) infrainguinal graft (synthetic, autologous, or single
outflow composite) or infrainguinal native artery. For native arteries, occlusions
of greater than or equal to 10 cm in length are eligible.
4. Diagnosis of occlusive thrombus in the graft or artery by arteriography after informed consent is obtained
5. Ability to access the thrombus with the infusion catheter and successfully embed
the infusion segment of the infusion catheter.
6. Subject must be able to give written informed consent prior to study entry
7. Age greater than or equal to 18 years
8. Women of child bearing potential must use adequate contraception for the duration
of the study and must have a negative pregnancy test prior to study entry
(Since there is no upper age limit defined for this trial for the CTRI registration purpose we have included the upper age limit to be considered as 99 years). 
 
ExclusionCriteria 
Details  Exclusion Criteria General 1. Any medical or social condition that may interfere with the subject successfully completing the study 2. Women who are pregnant or lactating, or first 10 days post-partum Past Medical History 3. Cardiopulmonary resuscitation in the last year 4. Previous systemic or anaphylactoid allergy to contrast agent, streptokinase, or blood products (subjects allergic to shellfish or iodine are permitted to enter the study). Contraindications To Thrombolysis 5. Ineligible for thrombolytic treatment for any reason. Specific exclusions include: a. History of hemorrhagic stroke b. Thrombotic or embolic stroke or cerebrovascular events (including transient ischemic attack [TIA]) within the past year c. Intracranial or spinal neuro-surgery or severe intracranial trauma in the past 3 months d. Major surgery, organ biopsy, or major trauma within the past 10 days e. Lumbar puncture or non-compressible arterial puncture in the past 10 days f. Intra-ocular surgery within the past 10 days g. Active gastrointestinal or organ bleeding. Minor bleeding such as normal menses, cystitis, or minor hemorrhoidal bleeding are not exclusions h. Uncontrolled arterial hypertension, defined as a systolic blood pressure >180 millimeters of mercury (mmHg) or diastolic blood pressure >110 mmHg. Thesubject will be eligible if the hypertension is controlled at the time of study enrollment. i. Known intracranial neoplasm, aneurysm, or arterio-venous malformation j. Current bleeding diathesis k. Platelet count <75 x 109/L Current Medical Status 6. Active graft infection 7. Occlusion occurred within one month of synthetic graft placement 8. Occlusion occurred within 6 months of autologous graft placement 9. A sequential composite graft with dual outflows to correct multiple occlusions 10. Deemed by the Investigator to be medically unable to tolerate open vascular procedure 11. Known prothrombotic state, e.g., anti-cardiolipin antibody, human immunodeficiency virus (HIV)-associated peripheral vascular disease 12. Known contraindication to heparin (e.g., history of heparin-induced thrombocytopenia) 13. Hemoglobin <10.0 g/dL (low hemoglobin at screening in the absence of active bleeding may be corrected by transfusion). Hemoglobin testing can be repeated. 14. Impaired renal function or renal disease that constitutes a contraindication to contrast arteriography, including a screen/baseline creatinine of >2.0 mg/dL. Creatinine may be repeated following hydration for prerenal azotemia. Prior/Concurrent Therapy 15. Previous treatment with Plasmin 16. Treatment with full dose plasminogen activator (e.g., streptokinase (e.g., Streptase®, Kabikinase®), anistreplase (Eminase®), alteplase (e.g., Activase®), reteplase (e.g., Retavase®), tenecteplase (TNKase?), urokinase (UK, [Abbokinase®]) within the last 48 hours 17. Treatment with a glycoprotein IIb/IIIa class of platelet inhibitor within 5 days prior to study entry or at any time during the study, e.g., abciximab (ReoPro®),eptifibatide (Integrilin®) or tirofiban (Aggrastat®) 18. Treatment with oral anticoagulants (e.g., warfarin, acenocumarol), and with an international normalized ratio (INR) of >1.7 (elevated INR at screening may be corrected prior to study enrollment). INR testing can be repeated. Others 19. Participation in another clinical study within 30 days prior to entry (imaging studies without investigative treatment are permitted), or concomitant participation in another study. 20. Mentally challenged adult subjects who cannot give independent written informed consent. 
 
Method of Generating Random Sequence   Computer generated randomization 
Method of Concealment   On-site computer system 
Blinding/Masking   Open Label 
Primary Outcome  
Outcome  TimePoints 
The proportion of subjects with 50% thrombolysis at the end of treatment compared to baseline by arteriography.  5 hr timepoint 
 
Secondary Outcome  
Outcome  TimePoints 
The incidence of major and minor bleeding events, deaths, adverse events, serious adverse events, and abnormal laboratory values as a measure of safety and tolerability.   DAY 30 
 
Target Sample Size   Total Sample Size="100"
Sample Size from India="20" 
Final Enrollment numbers achieved (Total)= "Applicable only for Completed/Terminated trials"
Final Enrollment numbers achieved (India)="Applicable only for Completed/Terminated trials" 
Phase of Trial   Phase 2 
Date of First Enrollment (India)   02/04/2011 
Date of Study Completion (India) Applicable only for Completed/Terminated trials 
Date of First Enrollment (Global)  10/12/2010 
Date of Study Completion (Global) Applicable only for Completed/Terminated trials 
Estimated Duration of Trial   Years="1"
Months="0"
Days="0" 
Recruitment Status of Trial (Global)
Modification(s)  
Open to Recruitment 
Recruitment Status of Trial (India)  Open to Recruitment 
Publication Details    
Individual Participant Data (IPD) Sharing Statement

Will individual participant data (IPD) be shared publicly (including data dictionaries)?  

Brief Summary   This is a Phase 2, multi-center, multi-national, randomized, open-label study (with blinded PA and PA Placebo groups) of Plasmin in subjects with acute angiographically confirmed lower extremity native artery or bypass graft occlusion. Subjects will be enrolled into one of six parallel treatment groups: four Plasmin Treatment groups differing only in administration technique and two blinded groups (a Blinded PA Treatment Group and a Blinded PA Placebo Group). The administration regimens for the Plasmin groups have been designed to examine the effect of different infusion catheter positions, different Plasmin pulse regimens, and different rates of Plasmin continuous infusion/volume on the thrombolytic activity and safety of Plasmin treatment. Acute lower extremity ischemia will be diagnosed by a PAO assessment examination for the purpose of classifying the subject into the Society for Vascular Surgery (SVS) acute limb ischemia category. This includes findings of sensory loss,muscle weakness, and presence or absence of arterial and venous Doppler signals (Doppler is optional at screening and baseline). Ischemic signs and symptoms of pain, paresthesias, paralysis, poikilothermia, pallor, and pulselessness will be documented. Approximately 100 subjects, ages of 18 or greater, are planned to be randomized in a 2:2:2:2:1:1 ratio to four Plasmin treatment groups (Groups A, B, C, and D, 20 subjects/group), one Blinded PA Treatment Group (Group E, 10 subjects/group), and one Blinded PA Placebo Group (Group F, 10 subjects/group) at investigative sites in multiple countries. Approximately eighty (80) subjects will be dosed with Plasmin, approximately ten (10) subjects will be dosed with PA and approximately ten (10) subjects will be dosed with PA Placebo. Subjects will be stratified at randomization into each treatment group by occluded lower extremity blood vessel type (native artery or graft) to ensure approximate equal balance of each presenting vessel type across treatment groups. Globally following countries are involved in this clinical trial: India, Sri Lanka, Belgium, Bulgaria, Czech Republic, Germany, Poland, Romania, Russia, Serbia, Slovakia, Spain, Ukraine, Brazil and Peru.  
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