It is an open-label, cross-sectional interventional tidy. The study is designed to find any biochemical changes that can be estimated in population which can differentiate them into stress resilient and stress susceptible individuals, and further into those that will develop or not develop depression.

The body coordinates the stress response by deploying multiple stress mediators viz; transmitters (e.g. norepinephrine / epinephrine/ serotonin), peptides (e.g. corticotrophin releasing factor, dynorphins), hormones (e.g. cortisol /humans and corticosterone/rodents, angiotensin). The complexity to an orchestrated stress response occurs at various levels, but majority of story revolves around levels of cortisol. The imbalances in cortisol levels are good predictors of over activated stress axis. The cross-play of this hormone is also appreciated in metabolic disorders particularly diabetes and obesity. In addition, recent involvement of adiponectin is a collagen-like plasma protein secreted by adipocytes is also suggested to play a substantial role in the development of insulin resistance, obesity and depression. The protein has been found to be decreased in cases of insulin resistance, diabetes, and depression, but what is the degree of reduction; the answer to this question is still unknown.

Thus, if we can identify how the levels of biochemical which can differentiate people with obese diabetic with and without depression, that can certainly help in diagnosis and prognosis of these co-morbid conditions.

 The most probable biochemicals that link all these three comorbid conditions are cortisol and adiponectin. It has been shown that adiponectin levels are lowered and cortisol level are increased in diabetes, obesity and depression cases individually, however how their levels in cases of co-morbid (all three disease conditions) conditions are affected in entirety and the information regarding modulation of adiponectin in diabetic and obese patients that will develop or not develop depression is unknown.

 After informed consent is obtained from eligible patients, patients will be asked to fill two questionnaires on patient health and stress inventory. All questions and doubts raised by patients will be answered appropriately. A blood sample will be obtained in the morning empty stomach to assess the levels of cortisol and adiponectin. Data on patients concomitant medicationand concurrent illnesses will be recorded.