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CTRI Number  CTRI/2018/05/014342 [Registered on: 31/05/2018] Trial Registered Retrospectively
Last Modified On: 31/05/2018
Post Graduate Thesis  No 
Type of Trial  BA/BE 
Type of Study    
Study Design  Randomized, Crossover Trial 
Public Title of Study   Bioequivalence study of Capecitabine tablets 500 mg  
Scientific Title of Study   An open label, balanced, randomized, two treatment, three sequence, threeperiod, single dose, Semi replicate, cross over, bioequivalence study of Capecitabine tablets 500 mg of Mega Pharma S.A. Chile and Xeloda® (Capecitabine) tablets 500 mg of Roche in metastatic cancer patients under fed conditions 
Trial Acronym   
Secondary IDs if Any  
Secondary ID  Identifier 
AZ/BE/04/17/11  Protocol Number 
 
Details of Principal Investigator or overall Trial Coordinator (multi-center study)  
Name  Dr Guansakaran 
Designation  Head - Clinical  
Affiliation  ​AZIDUS Laboratories Ltd 
Address  ​AZIDUS Laboratories Ltd., No.23rd School Road, Rathinamangalam, Behind Tagore Engg. College (Via) Vandalur, Kelambakkam Road, Chennai - 600 048

Kancheepuram
TAMIL NADU
600 048
India 
Phone  8056043873  
Fax    
Email  gunasakaran@azidus.com  
 
Details of Contact Person
Scientific Query
 
Name  Dr Guansakaran 
Designation  Head - Clinical  
Affiliation  ​AZIDUS Laboratories Ltd 
Address  ​AZIDUS Laboratories Ltd., No.23rd School Road, Rathinamangalam, Behind Tagore Engg. College (Via) Vandalur, Kelambakkam Road, Chennai - 600 048

Kancheepuram
TAMIL NADU
600 048
India 
Phone  8056043873  
Fax    
Email  gunasakaran@azidus.com  
 
Details of Contact Person
Public Query
 
Name  Dr Guansakaran 
Designation  Head - Clinical  
Affiliation  ​AZIDUS Laboratories Ltd 
Address  ​AZIDUS Laboratories Ltd., No.23rd School Road, Rathinamangalam, Behind Tagore Engg. College (Via) Vandalur, Kelambakkam Road, Chennai - 600 048

Kancheepuram
TAMIL NADU
600 048
India 
Phone  8056043873  
Fax    
Email  gunasakaran@azidus.com  
 
Source of Monetary or Material Support  
Mega Pharma S.A. Ruta 101, Km. 23.500, Parque de las Ciencias, Edificio Mega Pharma, Piso 3, 14000 Canelones, Uruguay 
 
Primary Sponsor  
Name  Mega Pharma SA 
Address  Mega Pharma S.A. Ruta 101, Km. 23.500, Parque de las Ciencias, Edificio Mega Pharma, Piso 3, 14000 Canelones, Uruguay 
Type of Sponsor  Pharmaceutical industry-Global 
 
Details of Secondary Sponsor  
Name  Address 
Azidus Laboratories Limited  No 23, School Road, Rathnamangalam, Chennai – 600 048 
 
Countries of Recruitment     India  
Sites of Study  
No of Sites = 2  
Name of Principal Investigator  Name of Site  Site Address  Phone/Fax/Email 
Dr krishna Kumar Rathnam  Meenakshi Mission Hospital & Research Centre  Meenakshi Mission Hospital & Research Centre Lake Area, Melur Road Madurai - 625107
Madurai
TAMIL NADU 
04524263000

kkrathnam@gmail.com 
Dr K C Lakshmiah  Srinivasam Cancer Care Multi Specialty Hospital India PVT LTD   Srinivasam Cancer Care Multi Specialty Hospital India PVT LTD #36, 1st A Main, 5th Cross (Nethravathi Street), Maruthi Nagar, Nagarbhavi Main Road, Bangalore - 5600072
Bangalore
KARNATAKA 
09448055949

kcluck@gmail.com 
 
Details of Ethics Committee  
No of Ethics Committees= 2  
Name of Committee  Approval Status 
Institutional Ethics Committee   Submittted/Under Review 
Srinivasam Cancer Care Multi Specialty Hospital Institutional Ethics Committee   Approved 
 
Regulatory Clearance Status from DCGI  
Status 
Not Applicable 
 
Health Condition / Problems Studied  
Health Type  Condition 
Patients  Metastatic cancer patients,  
 
Intervention / Comparator Agent  
Type  Name  Details 
Intervention  Capecitabine tablets 500 m  Capecitabine tablets 500 mg of Mega Pharma S.A. Chile 
Comparator Agent  Xeloda®  Capecitabine tablets 500 mg of Roche 
 
Inclusion Criteria  
Age From  18.00 Year(s)
Age To  60.00 Year(s)
Gender  Both 
Details  Patients of 18 to 60 years of age (both years inclusive) with established cases of cancer, who are already receiving a stable twice-daily dosing regimen in multiples of 500 mg tablet (i.e., twice daily, equivalent to 2500 mg/m2 total daily dose, for 2 weeks followed by a 1 week rest period given as 3 week cycles) as prescribed by the reference product label.

Patients whose body surface area is ≤ 1.25 m2, between 1.52 – 1.65 m2, between 1.92 – 2.05 m2 & dose is to be given in multiples of 500 mg tablet

Subjects who have no evidence of underlying disease (except Dukes’ C colon cancer/ metastatic colorectal carcinoma/ metastatic breast cancer) during screening medical history and whose physical examination is performed within 21 days prior to commencement of the study.

Patients who are taking Capecitabine as a single agent for adjuvant treatment for Dukes’ C colon cancer who have undergone complete resection of the primary tumor when treatment with fluoropyrimidine therapy alone is preferre

Patients who are taking Capecitabine as first-line treatment for metastatic colorectal carcinoma when treatment with fluoropyrimidine therapy alone is preferred.

Patients who are taking Capecitabine for the treatment of metastatic breast cancer resistant to both paclitaxel and an anthracycline containing chemotherapy regimen or resistant to paclitaxel and for whom further anthracycline therapy is not indicated, eg, patients who have received cumulative doses of 400 mg/m2 of doxorubicin or doxorubicin equivalents. (Only capecitabine as chemotherapeutic agent).

Patients should not take any adjuvant chemotherapeutic agent except capecitabine throughout the study and 4 weeks before the study.

Patients whose life expectancy of greater than or equal to 6 months.

Patients having histologically proven Cancer.

Patients having no brain metastasis.
 Patients with Performance ≤ 2 on the ECOG performance scale.

Subjects whose screening laboratory values are within normal limits or considered by the
Investigator/sub-Investigator to be of no clinical significance. Specifically, System Lab value ANC ≥ 1.5 × 103 /μl, Hb ≥ 10.0 g/dL, Platelets ≥ 1 lac/μl, Bilirubin ≤ 1.5 mg/dL, AST & ALT ≤ 2
x Upper Normal Limit or ≤ 5 × Upper Normal Limit (for liver mets)

Male Subjects must agree to comply with two highly effective contraceptive methods comprisinga barrier method (condom or occlusive cap plus spermicidal) for up to the last drug administration,and refrain from fathering a child for at least two (2) weeks following the last study drug administration. Periodic abstinence and withdrawal are not acceptable methods of contraception.

Female Subjects of child bearing potential practicing an acceptable method of birth control for the duration of the study as judged by the investigator(s), such as condoms, foams, jellies, diaphragm,intrauterine device (IUD), or abstinence or Postmenopausal for at least 1 year or

Surgically sterile (bilateral tubal ligation, bilateral oophorectomy, or hysterectomy has been performed on the subject) 
 
ExclusionCriteria 
Details  Allergy or Significant history of hypersensitivity or idiosyncratic reactions to Capecitabine and/or any related compounds etc.

Cardiovascular (including coronary artery disease), pulmonary, hepatic impairment, renal
(including severe renal impairment), hematological (including leucopenia, thrombocytopenia),
gastrointestinal, endocrine, immunologic, dermatologic, musculoskeletal, neurological, or
psychiatric disease.

Cancer patients with a prior history of coronary artery disease, receiving concomitant therapy of
warfarin.

Cancer patients with a history of dihydropyrimidine dehydrogenase deficiency.

Presence of infections which reduce life expectancy.

Alcohol dependence, alcohol abuse or drug abuse or addiction with any recreational drug within
past one year.

Undergoing concomitant oncologic treatment.
Smoking (≥ 10 cigarettes/day) or consumption of tobacco products (≥ 4 chews/day).

History of difficulty in swallowing or coming for follow up.

Clinically significant illness (except Dukes’ C colon cancer/ metastatic colorectal
carcinoma/metastatic breast cancer) within 4 weeks before the start of the study.

Subjects who have been on an abnormal diet (for whatever the reason) during the four weeks
preceding the study.

Female subject who is pregnant, lactating or likely to become pregnant or have a positive
pregnancy test at screening and prior to check in.

Positive result to HIV, HCV, RPR and HbsAg.

Use of enzyme-modifying drugs (like Phenytoin, Carbamazepine, Barbiturates, Gresiofulvine) in
the previous 30 days before day 1 of this study.

Abnormal 12 lead ECG, X-ray.
Donation of 350 mL or more of blood in the previous 90 days before day 1 of this study. Participation in another clinical trial within the preceding 90 days of study starts.


Subjects who have:

Systolic blood pressure less than 90 mm of Hg or more than 140 mm of Hg
Diastolic blood pressure less than 60 mm of Hg or more than 90 mm of Hg. Minor
deviations (2-4mm Hg) at check-in may be acceptable at the discretion of the investigator.
Pulse rate below 60/min or above 100/min.
 
 
Method of Generating Random Sequence   Computer generated randomization 
Method of Concealment   An Open list of random numbers 
Blinding/Masking   Open Label 
Primary Outcome  
Outcome  TimePoints 
To assess the bioequivalence between the test and reference formulations  To assess the bioequivalence between the test and reference formulations 
 
Secondary Outcome  
Outcome  TimePoints 
To Assess the safety  Screening and Post Study  
 
Target Sample Size   Total Sample Size="42"
Sample Size from India="42" 
Final Enrollment numbers achieved (Total)= "Applicable only for Completed/Terminated trials"
Final Enrollment numbers achieved (India)="Applicable only for Completed/Terminated trials" 
Phase of Trial   N/A 
Date of First Enrollment (India)   05/10/2017 
Date of Study Completion (India) Applicable only for Completed/Terminated trials 
Date of First Enrollment (Global)  Date Missing 
Date of Study Completion (Global) Applicable only for Completed/Terminated trials 
Estimated Duration of Trial   Years="1"
Months="0"
Days="0" 
Recruitment Status of Trial (Global)   Not Applicable 
Recruitment Status of Trial (India)  Open to Recruitment 
Publication Details   None 
Individual Participant Data (IPD) Sharing Statement

Will individual participant data (IPD) be shared publicly (including data dictionaries)?  

Brief Summary  

An open label, balanced, randomized, two treatment, three sequence, three period, single dose, Semi replicate, cross over, bioequivalence study of Capecitabine tablets 500 mg of Mega Pharma S.A. Chile and Xeloda® (Capecitabine) tablets 500 mg of Roche in metastatic cancer patients under fed conditions.

Test Product-T : Capecitabine tablets 500 mg of Mega Pharma S.A. Chile Reference Product-R : Xeloda® (Capecitabine) tablets 500 mg of Roche

Objective : To assess the bioequivalence between the test and reference formulations.

Study design : An open label, balanced, randomized, two treatment, three sequence, three period, single dose, Semi replicate, cross over, bioequivalence study

Number of Subjects : Metastatic cancer patients who are already receiving a stable twice-daily dosing regimen (i.e. 1250 mg/m2 , twice daily, equivalent to 2500 mg/m2 total daily dose, for 2 weeks followed by a 1 week rest period given as 3 week cycles) shall be recruited in order to complete the study with at least 42 patients. These patients shall be dosed in Gi, i= 1, 2, 3... Clinical groups.

 Type of study : Fed Number of periods : 03

Washout period : The test and reference products will be dosed on three consecutive days. Since the patients are on a twice daily dosing regimen, there will be ~12 hours interval between the evening dosing in each period and subsequent morning dosing. Subjects will be housed continuously for both the periods since the dosing will be done with 24 hours interval between each dose.

Fasting criteria : Minimum 08 hours fasting prior to high fat high calorie breakfast and 04 hours post dose.

Water restriction : 01 hour pre-dose and 01 hour post-dose

 Drug Administration : In each of the periods, patients will receive tablets (as multiples of the 500 mg tablet) of test or reference product of Capecitabine 500 mg tablet as per their BSA (Body surface Area), using Mosteller formula. The tablets will be given with 200 ml (± 2 ml) of water at ambient temperature, 30 minutes after administration of high calorie high fat breakfast in the morning on Day 1 (period I), Day 2 (period II) and Day 3 (period III) of the treatment cycle, after an overnight fasting of 08 hours (for each day) as per the randomization schedule. Dosing will be done by trained person under the supervision of investigator. After dosing, mouth check will be done to assess dosing compliance. Similarly on day 1 (period I), day 2 (period II), and Day 3 (Period III) evening dose of Capecitabine 500mg tablets (marketed product) will be administered. For evening dose, the patients shall receive their usual dose of capecitabine tablet as per their current dosing regimen.

Postural restrictions : Subjects will be administered with study medications in sitting posture. Subjects will remain seated for 04 hours after dosing. During this period, the subjects can be seated or ambulatory.

Clinical confinement : From at least 12.00 hours prior to drug administration in Day 1 and until 12.00 hours post dosing in Day 3.

Safety assessment : Vital signs measurement and subject well-being assessment will be done during subject check-in, housing and during check out.

 Laboratory assessments : Screening – Hematology, Biochemistry, Serology, Urine analysis, ECG, Chest X ray, Serum pregnancy test (for female volunteers). Check-In: Alcohol breath analysis, urine drugs of abuse and urine pregnancy test (for female volunteers)

 Post study safety analysis (At the end of study) – Hematology and biochemistry and Serum pregnancy test (for female volunteers)

 Sampling Schedule in each period : 00.00 (Pre-dose), 00.17, 00.33, 00.50, 00.67, 00.83, 01.00, 01.25, 01.50, 01.75, 02.00, 02.33, 02.67, 03.00, 03.33, 03.67, 04.00, 04.50, 05.00, 06.00, 08.00 and 10.00 hours post-dose (Total of 22 samples - 3 ml each). All the samples will be collected inside the clinic.

Total amount of blood draw : The total volume of blood draw from each subject during the study will not exceed 245.0 ml.

Analytes : Capecitabine in plasma.

Bio-analytical procedure : A validated LC-MS/MS bio-analytical method will be used for estimation of Capecitabine in plasma.

 Pharmacokinetic analysis and parameters : Pharmacokinetic analysis will be done using Phoenix® WinNonlin v 7.0. Primary PK parameters: Cmax, AUC0-t and AUC0-∞ Secondary PK parameters: Tmax, VD, CL, T½, Kel and AUC0-t / AUC0-∞ X 100

Statistical analysis : Statistical analysis will be performed on the pharmacokinetic parameters using SAS® v 9.4. Statistical analysis will be performed for the first 12 subjects. Based on the outcome of the study results, the remaining subjects will be recruited and dosed.

 
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