1. What data in particular will be shared?
   Response - All of the individual participant data collected during the trial, after de-identification.
   


2. What additional supporting information will be shared?
   Response - Informed Consent Form
   
   
3. Who will be able to view these files?
   Response - Researchers whose proposed use of the data has been approved by an independent review committee identified for this purpose.
   


4. For what types of analyses will this data be available?
   Response - To achieve aims in the approved proposal.
   


5. By what mechanism will data be made available?
   Response (Others) -  In the form of informed Consent


6. For how long will this data be available start date provided 25-02-2026 and end date provided 01-01-2036?
   Response - Immediately following publication. No end date.
   


7. Any URL or additional information regarding plan/policy for sharing IPD? 
   Additional Information - NIL

Root canal infections are polymicrobial in nature and are a major cause of failure of endodontic treatment. Mechanical instrumentation alone is insufficient to completely eliminate microorganisms from the complex root canal anatomy, necessitating the use of effective irrigating solutions. Chlorhexidine is a commonly used irrigant due to its broad-spectrum antimicrobial activity and substantivity; however, it lacks tissue-dissolving properties and may be less effective against resistant microorganisms. Antibiotic combinations such as amoxyclav, ciprofloxacin, and metronidazole offer broader antimicrobial coverage and may enhance root canal disinfection.This randomized controlled clinical trial aims to comparatively evaluate the antimicrobial efficacy of 2% chlorhexidine, 2% chlorhexidine with metronidazole (0.5%), amoxyclav (1.2 g) with metronidazole (0.5%), and ciprofloxacin (0.2%) with metronidazole (0.5%) as root canal irrigants. A total of 60 patients requiring primary root canal treatment in single-rooted permanent teeth diagnosed with pulp necrosis and/or chronic apical periodontitis will be recruited and randomly allocated into four groups. All cases will undergo standardized chemomechanical preparation using 5.25% sodium hypochlorite and 17% EDTA, followed by final irrigation with the assigned study solution.Microbiological samples will be collected before and after irrigation using sterile paper points, and intracanal bacterial load will be assessed by culture techniques and expressed as colony-forming units per milliliter (CFU/ml). The primary outcome measure will be the reduction in intracanal microbial load, while secondary outcomes will include intergroup comparison of antimicrobial efficacy, percentage reduction in bacterial counts, reduction in Enterococcus faecalis, and incidence of adverse events. The results of this study may help identify a more effective irrigation regimen for enhanced root canal disinfection.