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CTRI Number  CTRI/2026/01/102198 [Registered on: 27/01/2026] Trial Registered Prospectively
Last Modified On:
Post Graduate Thesis  No 
Type of Trial  BA/BE 
Type of Study   NA 
Study Design  Randomized, Crossover Trial 
Public Title of Study   To evaluate the absorption of Riociguat 2.5 mg tablets (T) compared to ADEMPAS® (riociguat) 2.5 mg tablets (R) in normal healthy, adult male subjects under fasting condition. 
Scientific Title of Study   An open label, randomized, balanced, two treatment, two sequence, two period, two-way crossover, single-dose, oral bioequivalence study of Riociguat 2.5 mg tablets (T) manufactured by Zenara Pharma Pvt. Ltd., Plot No: 83/B, 84 & 87-96, Phase III, IDA Cherlapally, Hyderabad-500051, India with ADEMPAS® (riociguat) 2.5 mg tablets (R) manufactured for Bayer HealthCare Pharmaceuticals Inc. Whippany, NJ 07981, United states in normal healthy, adult male subjects under fasting condition. 
Trial Acronym  NIL 
Secondary IDs if Any  
Secondary ID  Identifier 
S-25-901, Version 01, dated 21-Jul-2025, Amd-01  Protocol Number 
 
Details of Principal Investigator or overall Trial Coordinator (multi-center study)  
Name  Dr Harisha C 
Designation  Principal Investigator 
Affiliation  Notrox Research Pvt Ltd 
Address  No 19/3, 2nd Floor, Bikasipura road, JC Industrial layout,
off Kanakapura Road, (Behind Metro Cash and carry),
Bangalore
KARNATAKA
560062
India 
Phone  08026861077  
Fax  -  
Email  harisha-c@notroxresearch.com  
 
Details of Contact Person
Scientific Query
 
Name  Dr Harisha C 
Designation  Principal Investigator 
Affiliation  Notrox Research Pvt Ltd 
Address  No 19/3, 2nd Floor, Bikasipura road, JC Industrial layout,
off Kanakapura Road, (Behind Metro Cash and carry),

KARNATAKA
560062
India 
Phone  08026861077  
Fax  -  
Email  harisha-c@notroxresearch.com  
 
Details of Contact Person
Public Query
 
Name  Dr Harisha C 
Designation  Principal Investigator 
Affiliation  Notrox Research Pvt Ltd 
Address  No 19/3, 2nd Floor, Bikasipura road, JC Industrial layout,
off Kanakapura Road, (Behind Metro Cash and carry),

KARNATAKA
560062
India 
Phone  08026861077  
Fax  -  
Email  harisha-c@notroxresearch.com  
 
Source of Monetary or Material Support  
Zenara Pharma Pvt. Ltd. Plot No: 83/B, 84 & 87-96 Phase III, IDA Cherlapally, Hyderabad-500051, India. 
 
Primary Sponsor  
Name  Zenara Pharma Pvt. Ltd. 
Address  Plot No: 83/B, 84 & 87-96 Phase III, IDA Cherlapally, Hyderabad-500051, India. 
Type of Sponsor  Pharmaceutical industry-Indian 
 
Details of Secondary Sponsor  
Name  Address 
NIL   
 
Countries of Recruitment     India  
Sites of Study  
No of Sites = 1  
Name of Principal Investigator  Name of Site  Site Address  Phone/Fax/Email 
Dr Harisha C MBBS  Notrox Research Pvt Ltd  No 19/3, 3rd Floor, Bikasipura Main Rd, JC Industrial Layout
Bangalore
KARNATAKA 
080-26861077
-
harisha-c@notroxresearch.com 
 
Details of Ethics Committee  
No of Ethics Committees= 1  
Name of Committee  Approval Status 
Shettys Oncology and Multispeciality Hospital Ethics Committee  Approved 
 
Regulatory Clearance Status from DCGI  
Status 
Approved/Obtained 
 
Health Condition / Problems Studied  
Health Type  Condition 
Healthy Human Volunteers  Healthy Human Volunteers 
 
Intervention / Comparator Agent  
Type  Name  Details 
Comparator Agent  ADEMPAS (riociguat) 2.5 mg tablets (R) manufactured for Bayer HealthCare Pharmaceuticals Inc. Whippany, NJ 07981, United states  After an overnight fasting of at least 10.00 hours, a single dose of ADEMPAS® (riociguat) 2.5 mg tablets (R) manufactured for Bayer HealthCare Pharmaceuticals Inc. Whippany, NJ 07981, United states will be administered along with 240±2 mL of water, will be administered orally to the subjects in sitting posture at ambient temperature in the morning, as per the randomization schedule. 
Intervention  Riociguat 2.5 mg tablets (T) manufactured by Zenara Pharma Pvt. Ltd., Plot No: 83/B, 84 and 87-96, Phase III, IDA Cherlapally, Hyderabad-500051, India  After an overnight fasting of at least 10.00 hours, a single dose of Riociguat 2.5 mg tablets (T) manufactured by Zenara Pharma Pvt. Ltd., Plot No: 83/B, 84 and 87-96, Phase III, IDA Cherlapally, Hyderabad-500051, India will be administered along with 240±2 mL of water, will be administered orally to the subjects in sitting posture at ambient temperature in the morning, as per the randomization schedule. 
 
Inclusion Criteria  
Age From  18.00 Year(s)
Age To  45.00 Year(s)
Gender  Male 
Details  Volunteers who accepts for participating in this study must be:
1) Healthy, adult male, subjects aged between 18-45 years (both inclusive) weighing at least 50 kg at the time of screening.
2) Having a Body Mass Index (BMI) between 18.50 to 29.99 kg/m2 (both inclusive) at the time of screening.
3) Normal or clinically insignificant findings during screening, medical history, medical examination, laboratory evaluations, 12 lead ECG and X-ray chest (posterior-anterior view) recordings.
4) Able to comply with the study procedures, in the opinion of the Principal Investigator.
5) Compliance with study specific restrictions and prohibitions.
6) Able to give voluntary written informed consent for participation in the trial.
 
 
ExclusionCriteria 
Details  If any subject is having any of the following conditions, then exclude him from participation in this study:
1) Known hypersensitivity or idiosyncratic reaction to the study drug or any related drug.
2) Subjects with a history of previous use of medicines like ACE and ARB inhibitor.
3) History or presence of any disease or disorder known to influence bone metabolism, compromise the hemopoietin, renal, hepatic, endocrine, pulmonary, central nervous, cardiovascular, immunological, dermatological, gastrointestinal, musculoskeletal or any other body system.
4) Systolic blood pressure less than 90 mmHg or more than 140 mmHg at the time of screening
5) Diastolic blood pressure less than 60 mmHg or more than 90 mmHg at the time of screening.
6) Pulse rate below 60 beats/minute or above 100 beats/minute at the time of screening.
7) Respiratory rate below 12 or above 20 breaths per minute at the time of screening.
8) Body temperature below 96.2o F or above 99.8o F at the time of screening.
9) SpO2 less than 94% at the time of screening.
10) Use of any prescribed medication (including herbal remedies and vitamins) during the two weeks before the start of the study or OTC medicinal products (including herbal remedies and vitamins) during one week prior to study initiation and throughout the study. In any such case subject selection will be at the discretion of the Principal Investigator.
11) Habit of consuming high caffeine (more than 5 cups of coffee or tea/day).
12) Smokers and Alcoholics.
13) History of dehydration from diarrhea, vomiting or any other reason within a period of 24.00 hours prior to study check-in.
14) An unusual or abnormal diet within 48.00 hours prior to study check-in, whatever reason e.g. because of fasting due to religious reasons.
15) The presence of clinically significant abnormal laboratory values during screening.
16) Use of any recreational drugs or history of drug addiction or testing positive in pre-study Urine drug screening and Alcohol breath analyzer test.
17) A history of difficulty with donating blood or having donated blood in the preceding 90 days for males prior to the start of the study.
18) Subject who has participated in any other clinical study involving drug administration and collection of blood samples in the 90 days for males preceding the start of the study.
19) Difficulty in swallowing tablets.
20) Positive HIV, VDRL/RPR, Hepatitis B and C tests.
21) Subjects who have used any drugs or substances known to be strong inhibitors or inducers of Cytochrome P450 enzymes within 14 days prior to IP administration in period I. 
 
Method of Generating Random Sequence   Computer generated randomization 
Method of Concealment   Centralized 
Blinding/Masking   Open Label 
Primary Outcome  
Outcome  TimePoints 
Cmax, AUC0-t and AUC0-inf  Total 22 blood samples in each period, a single pre-dose (-01.00 to 00.00) blood sample of 4.0 mL will be collected and placed in wet ice pack eachperiod. The pre-dose and post-dose blood samples will be collected in pre-labeled K2EDTA vacutainers. The post-dose blood samples of 4.0 mL will be collected and placed in wet ice pack at 00.25, 00.50, 00.75, 01.00, 01.25, 01.50, 01.75, 02.00, 02.50, 03.00, 03.50, 04.00, 04.50, 05.00, 06.00, 08.00, 10.00, 12.00, 16.00, 24.00 and 48.00 hours post-dose. 
 
Secondary Outcome  
Outcome  TimePoints 
Tmax, t½, Kel, Kel Lower, Kel Upper and AUCExtrapolated%  otal 22 blood samples in each period, a single pre-dose (-01.00 to 00.00) blood sample of 4.0 mL will be collected and placed in wet ice pack eachperiod. The pre-dose and post-dose blood samples will be collected in pre-labeled K2EDTA vacutainers. The post-dose blood samples of 4.0 mL will be collected and placed in wet ice pack at 00.25, 00.50, 00.75, 01.00, 01.25, 01.50, 01.75, 02.00, 02.50, 03.00, 03.50, 04.00, 04.50, 05.00, 06.00, 08.00, 10.00, 12.00, 16.00, 24.00 and 48.00 hours post-dose. 
 
Target Sample Size   Total Sample Size="46"
Sample Size from India="46" 
Final Enrollment numbers achieved (Total)= "Applicable only for Completed/Terminated trials"
Final Enrollment numbers achieved (India)="Applicable only for Completed/Terminated trials" 
Phase of Trial   N/A 
Date of First Enrollment (India)   10/02/2026 
Date of Study Completion (India) Applicable only for Completed/Terminated trials 
Date of First Enrollment (Global)  Date Missing 
Date of Study Completion (Global) Applicable only for Completed/Terminated trials 
Estimated Duration of Trial   Years="1"
Months="0"
Days="0" 
Recruitment Status of Trial (Global)   Not Yet Recruiting 
Recruitment Status of Trial (India)  Not Yet Recruiting 
Publication Details   N/A 
Individual Participant Data (IPD) Sharing Statement

Will individual participant data (IPD) be shared publicly (including data dictionaries)?  

Response - NO
Brief Summary  
After an overnight fasting of at least 10.00 hours, a single dose of Riociguat 2.5 mg tablets (T) manufactured by Zenara Pharma Pvt. Ltd., Plot No: 83/B, 84 & 87-96, Phase III, IDA Cherlapally, Hyderabad-500051, India or ADEMPAS® (riociguat) 2.5 mg tablets (R) manufactured for Bayer HealthCare Pharmaceuticals Inc. Whippany, NJ 07981, United states will be administered along with 240±2 mL of water, will be administered orally to the subjects in sitting posture at ambient temperature in the morning, as per the randomization schedule.
Subjects will receive the alternate ‘treatment’ in the subsequent periods, in such a way that each subject will have received all the ‘treatments’ by the end of the study.
 
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