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CTRI Number  CTRI/2026/01/100635 [Registered on: 09/01/2026] Trial Registered Prospectively
Last Modified On: 09/01/2026
Post Graduate Thesis  Yes 
Type of Trial  Interventional 
Type of Study   Dentistry 
Study Design  Randomized, Parallel Group, Active Controlled Trial 
Public Title of Study   Comparison of Modified Vestibular Incision Subperiosteal Tunnel Technique and Advanced Platelet Rich Fibrin Plus with Low Level Laser Therapy in the treatment of receding gums 
Scientific Title of Study   Comparison of Modified Vestibular Incision Subperiosteal Tunnel Technique (MVISTA) with Advanced Platelet Rich Fibrin Plus (A-PRF+) with and without use of adjunctive Low Level Laser Therapy (LLLT) in management of Cairo’s RT1 gingival recessions: A Randomized Clinical Trial 
Trial Acronym  NIL 
Secondary IDs if Any  
Secondary ID  Identifier 
NIL  NIL 
 
Details of Principal Investigator or overall Trial Coordinator (multi-center study)  
Name  Dr Ankita Chakraborty 
Designation  Postgraduate 
Affiliation  Manipal College Of Dental Sciences Mangalore  
Address  Department Of Periodontology 4th Floor Manipal College Of Dental Sciences Mangalore Affiliated To Manipal Academy Of Higher Education, Manipal Karnataka Dakshina Kannada Karnataka 575001 India

Dakshina Kannada
KARNATAKA
575001
India 
Phone  9430993537  
Fax    
Email  chakrabortyank99@gmail.com  
 
Details of Contact Person
Scientific Query
 
Name  Dr Deepa G Kamath 
Designation  PROFESSOR 
Affiliation  Department Manipal College Of Dental Sciences Mangalore  
Address  Department Of Periodontology 4th Floor Manipal College Of Dental Sciences Mangalore Affiliated To Manipal Academy Of Higher Education, Manipal Karnataka Dakshina Kannada Karnataka 575001 India

Dakshina Kannada
KARNATAKA
575001
India 
Phone  9448454788  
Fax    
Email  deepa.gkamath@manipal.edu  
 
Details of Contact Person
Public Query
 
Name  Dr Deepa G Kamath 
Designation  Professor 
Affiliation  Manipal College Of Dental Sciences Mangalore  
Address  Department Of Periodontology 4th Floor Manipal College Of Dental Sciences Mangalore Affiliated To Manipal Academy Of Higher Education, Manipal Karnataka Dakshina Kannada Karnataka 575001 India

Dakshina Kannada
KARNATAKA
575001
India 
Phone  9448454788  
Fax    
Email  deepa.gkamath@manipal.edu  
 
Source of Monetary or Material Support  
NIL 
 
Primary Sponsor  
Name  Dr Ankita Chakraborty 
Address  Department Of Periodontology 4th Floor Manipal College Of Dental Sciences Mangalore Affiliated To Manipal Academy Of Higher Education, Manipal Karnataka Dakshina Kannada Karnataka 575001 India  
Type of Sponsor  Other [self] 
 
Details of Secondary Sponsor  
Name  Address 
NIL  NIL 
 
Countries of Recruitment     India  
Sites of Study  
No of Sites = 1  
Name of Principal Investigator  Name of Site  Site Address  Phone/Fax/Email 
Dr Ankita Chakraborty  Depar Manipal College Of Dental Sciences Mangalore  Department Of Periodontology 4th Floor Manipal College Of Dental Sciences Mangalore Affiliated To Manipal Academy Of Higher Education, Manipal Karnataka Dakshina Kannada Karnataka 575001 India
Dakshina Kannada
KARNATAKA 
9430993537

chakrabortyank99@gmail.com 
 
Details of Ethics Committee  
No of Ethics Committees= 1  
Name of Committee  Approval Status 
Institutional Ethics Committee Manipal College of Dental Sciences, Mangalore  Approved 
 
Regulatory Clearance Status from DCGI  
Status 
Not Applicable 
 
Health Condition / Problems Studied  
Health Type  Condition 
Healthy Human Volunteers  Systemically healthy patients 
 
Intervention / Comparator Agent  
Type  Name  Details 
Comparator Agent  Modified Vestibular Incision Sub-Periosteal Tunnel Access with Advanced Platelet-rich Fibrin Plus   To compare the clinical efficacy of Modified Vestibular Incision SubPeriosteal Tunnel Access with Advanced Platelet-rich Fibrin Plus followed by Low Level Laser Therapy,and Modified Vestibular Incision Sub-Periosteal Tunnel Access with Advanced Platelet-rich Fibrin Plus in the management of Cairo’s RT1 gingival recessions with 6 months follow up  
Intervention  To compare the clinical efficacy of Modified Vestibular Incision Sub-Periosteal Tunnel Access with Advanced Platelet-rich Fibrin Plus followed by Low Level Laser Therapy  To compare the clinical efficacy of Modified Vestibular Incision SubPeriosteal Tunnel Access with Advanced Platelet-rich Fibrin Plus followed by Low Level Laser Therapy,and Modified Vestibular Incision Sub-Periosteal Tunnel Access with Advanced Platelet-rich Fibrin Plus in the management of Cairo’s RT1 gingival recessions with 6 months follow up  
 
Inclusion Criteria  
Age From  18.00 Year(s)
Age To  60.00 Year(s)
Gender  Both 
Details  Patients who agree to participate in the study by signing the informed consent
Presence of more than or equal to 2mm adjacent Cairo’s RT1 gingival recessions on aesthetic zones including premolars in the maxillary or mandibular arch with an apico-coronal extension
Probing depth less than 3 mm
Full mouth plaque index score less than 20 percent
Gingival thickness more than or equal to 2mm
 
 
ExclusionCriteria 
Details  Patients with systemically compromised health
Previous periodontal surgical treatment in the involved site
Pregnant or lactating mothers
Patient with smoking habit
Non compliant patients
Restorations and superficial caries in the area to be treated
Mobile or fully restored teeth
 
 
Method of Generating Random Sequence   Computer generated randomization 
Method of Concealment   Sequentially numbered, sealed, opaque envelopes 
Blinding/Masking   Participant Blinded 
Primary Outcome  
Outcome  TimePoints 
Recession depth
Recession width
Probing depth
Clinical attachment level
Keratinized tissue height
Gingival thickness
Mean Root Coverage
Complete Root Coverage
 
Baseline
1 month
3 month
6 month
 
 
Secondary Outcome  
Outcome  TimePoints 
Early Wound Healing Scores  24 hours postoperative  
Root Coverage Esthetic Score  6 month postoperative  
Plaque index   baseline
6 month 
Modified sulcular bleeding index   baseline
6 months 
Patient Reported Outcome Measures  3 months
6 months 
 
Target Sample Size   Total Sample Size="16"
Sample Size from India="16" 
Final Enrollment numbers achieved (Total)= "Applicable only for Completed/Terminated trials"
Final Enrollment numbers achieved (India)="Applicable only for Completed/Terminated trials" 
Phase of Trial   N/A 
Date of First Enrollment (India)   03/04/2026 
Date of Study Completion (India) Applicable only for Completed/Terminated trials 
Date of First Enrollment (Global)  Date Missing 
Date of Study Completion (Global) Applicable only for Completed/Terminated trials 
Estimated Duration of Trial   Years="2"
Months="6"
Days="0" 
Recruitment Status of Trial (Global)   Not Applicable 
Recruitment Status of Trial (India)  Not Yet Recruiting 
Publication Details   N/A 
Individual Participant Data (IPD) Sharing Statement

Will individual participant data (IPD) be shared publicly (including data dictionaries)?  

Response - NO
Brief Summary  

Gingival recession is defined as the displacement of the marginal tissue apical to the cementoenamel junction  resulting in exposure of the root surface and potentially creating aesthetic and functional problems such as dentine hypersensitivity and root caries It affects populations worldwide, occurring across all age groups with multiple etiological factors contributing to its development, and prevalence is seen to increase with age Common etiological factors are inadequate oral hygiene faulty toothbrushing, smoking or chewing tobacco and malocclusion among others  The classification of gingival recession has evolved significantly over time aimed at improving diagnosis prognosis and treatment planning The earliest classification by Sullivan and Atkins in 1968 categorized recessions based on the depth and width of the defect into four types deep wide shallow wide deep narrow and shallow narrow This was followed by Miller classification in 1985 which became the most widely used system Miller divided recessions into four classes based on the extent of recession relative to the mucogingival junction  and the presence or absence of interdental bone and soft tissue However Miller classification of gingival recession has several drawbacks including difficulty locating the MGJ  lack of information on keratinized tissue vague criteria for interdental bone loss and inability to classify palatal recessions or cases where interdental bone loss occurs without recession extending beyond the MGJ. Moreover, its prognostic predictions are often inaccurate, as clinical outcomes do not always conform to Miller’s expectations for root coverage. To address these shortcomings, newer classifications were introduced. For example, in 1998, Nordland and Tarnow proposed a classification based on the height of interdental papillae. The Cairo classification, introduced in 2011, utilized interproximal clinical attachment level as a more objective and reproducible criterion. The Cairo system categorizes recessions into three types depending on the severity of interproximal attachment loss relative to buccal attachment loss. RT1 Gingival recession with no loss of interproximal attachment. Interproximal CEJ was clinically not Detectable at both mesial and distal aspects of the tooth. RT2: Gingival recession associated with loss of interproximal attachment. The amount of interproximal attachment Loss was less than or equal to the Buccal attachment loss RT3: Gingival recession associated with loss of interproximal attachment. The amount of interproximal attachment Loss was Higher than the buccal attachment loss. This allows a clearer distinction among recession types and more accurate prediction of root coverage outcomes. Studies have shown that Cairo classification is more reliable, with higher intra- and inter-examiner agreement than Miller’s, and offers a clearer, treatment-oriented approach by focusing on attachment loss rather than the variable MGJ This makes the Cairo classification more practical and precise for diagnosis, prognosis, and treatment planning in periodontology. Other contemporary modifications and new systems incorporate details such as keratinized tissue, tooth malposition, and palatal recessions to provide a more comprehensive clinical picture.The primary objectives of treating gingival recession are to achieve root coverage, restore esthetics, facilitate plaque control and reduce hypersensitivity Tunnel techniques represent a minimally invasive surgical approach to treat multiple gingival recessions by creating a subperiosteal tunnel through a single incision, allowing graft placement without disrupting the blood supply to the flap. The absence of vertical incisions and papilla detachment provides better blood supply, promotes post-operative healing and  reduces visible scarringThe Vestibular Incision Subperiosteal Tunnel Access  technique is an advancement in tunnel procedures. It utilises a vestibular incision to enhance flap mobilization, thus covering multiple recession defects with minimal trauma The Modified Vestibular Incision Subperiosteal Tunnel Access  technique differs from the VISTA technique mainly with respect to incision design, the supra-periosteal tunnel, graft material used and the method of suturing. In MVISTA, a single vestibular incision is used to establish a wider mucoperiosteal tunnel for complete elevation and passage of grafts, thus reducing trauma and increasing flap mobility These modifications enhance access, facilitate multiple root coverage at once, and have been seen to achieve more predictable and stable long term clinical results in gingival recession treatment. Platelet-Rich Fibrin is a second-generation autologous platelet concentrate platelet rich fibrin is simple to prepare and affordable since it requires no additional activation factors or anticoagulants. Its fibrous structure forms a three-dimensional fibrin scaffold that retains more cytokines and growth factors, releasing them gradually over 10 days, creating a natural, sustained healing environment. Additionally platelet rich fibrin dissolves more slowly in tissue, forming a solid fibrin matrix resembling a natural blood clot, for better tissue integration.Thus .platelet rich fibrin promotes tissue healing and regeneration, and when combined with  Vestibular Incision Subperiosteal Tunnel Access  technique  improves biotype and healing of tissue and covers multiple recession areas well  Third generation PRF Advanced Platelet-Rich Fibrin Plus  offers superior healing and regenerative benefits over earlier platelet rich fibrin generations, mainly due to its enhanced release of growth factors achieved by slower and shorter centrifugation processes. Studies consistently show that Advanced Platelet-Rich Fibrin Plus improves bone formation supports gingival and periodontal healing, and enhances outcomes in bone graft integration and ridge preservation when compared with both conventional platelet rich fibrin and other blood-derived biomaterials. The increased concentration of key growth factors and cytokines in A-PRF+ results in faster neoangiogenesis, more efficient cellular responses, and better maintenance of tissue dimensions at surgical sites, making it a preferred adjunct in contemporary periodontal and oral surgical procedures. Advanced Platelet-Rich Fibrin Plus  demonstrates superior clinical performance compared to earlier platelet rich fibrin generations, primarily due to increased release of growth factors and cytokines that promote tissue healing and regeneration, with studies showing improved bone formation, better graft integration, and enhanced periodontal outcomes in various surgical protocols.   Photobiomodulation refers to the therapeutic use of low-level laser light to stimulate cellular activity and enhance tissue repair without causing thermal damage. Low-Level Laser Therapy  is a form of photobiomodulation applied in periodontal treatment to reduce inflammation, pain, and promote wound healing, often used adjunctively with surgical techniques to improve clinical results. Low level laser therapy supports post-surgical healing by employing low-intensity laser light to stimulate cellular processes at the wound site, enhancing tissue repair and regeneration without causing thermal damageLow-Level Laser Therapy accelerates healing by promoting mitochondrial activity and ATP production, which boosts cellular energy and facilitates fibroblast proliferation, angiogenesis, and collagen synthesis essential for soft tissue recovery after surgery. Low-level laser therapy  sterilizes tissue surfaces, modulating inflammatory response and reducing the risk of bacteremia and minimizing swelling, edema, and scarring by decreasing inflammatory cytokines, improving lymphatic drainage, and lowering vascular permeability ClinicallyLow-Level Laser Therapy has been shown to significantly decrease postoperative pain and the consumption of analgesics, optimize tissue repair, and promote wound closure, giving it a valuable adjunctive role in the post-surgical management of gingival recessions and periodontal procedures 

 
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