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CTRI Number  CTRI/2017/10/010040 [Registered on: 06/10/2017] Trial Registered Retrospectively
Last Modified On: 06/10/2017
Post Graduate Thesis  No 
Type of Trial  Interventional 
Type of Study   Biological 
Study Design  Randomized, Crossover Trial 
Public Title of Study   PK and PD study of G-CSF (BioGenomics Ltd) vs Neupogen® in healthy volunteers  
Scientific Title of Study   A Phase I, Single-centre, Randomized, Double-Blind, Two Treatment, Two-Period, Two-sequence, Two-way Crossover Study to Demonstrate Equivalence of Pharmacokinetic and Pharmacodynamic Characteristics and to Compare Safety and Tolerability of Granulocyte Colony Stimulating Factor (BioGenomics Limited) and Neupogen® Administered via Subcutaneous Route as Multiple Consecutive Doses in Healthy Adult Human Volunteers 
Trial Acronym   
Secondary IDs if Any  
Secondary ID  Identifier 
BGL/GCSF/PROTOCOL/IN/01-15 Version 3 dated 31 July 2015   Protocol Number 
 
Details of Principal Investigator or overall Trial Coordinator (multi-center study)  
Name  Dr Hiren Prajapati 
Designation  Principal Investigator 
Affiliation  Veeda Clinical Research 
Address  Veeda Clinical Research Pvt. Ltd. Shivalik Plaza Wing-A, Near IIM, Ambawadi, Ahmedabad – 380 015, India

Ahmadabad
GUJARAT
380 015
India 
Phone  9179-30013000  
Fax  9179-30013010  
Email  hiren.prajapati@veeda.com  
 
Details of Contact Person
Scientific Query
 
Name  Dr Chandrashekhar Bolmall MD 
Designation  Medical Director- Clinical Research  
Affiliation  BioGenomics Limited 
Address  BioGenomics Limited, First Floor, Kothari Compound, Opp. Tikuji ni wadi, Thane (W)-400610, Maharashtra, India
BioGenomics Limited, First Floor, Kothari Compound, Opp. Tikuji ni wadi, Thane (W)-400610, Maharashtra, India
Thane
MAHARASHTRA
400610
India 
Phone  022-41617181  
Fax  022-41617199  
Email  chandrashekhar.bolmall@biogenomics.co.in  
 
Details of Contact Person
Public Query
 
Name  Dr Chandrashekhar Bolmall MD 
Designation  Medical Director-Clinical Research  
Affiliation  BioGenomics Limited 
Address  BioGenomics Limited, First Floor, Kothari Compound, Opp. Tikuji ni wadi, Thane (W)-400610, Maharashtra, India
BioGenomics Limited, First Floor, Kothari Compound, Opp. Tikuji ni wadi, Thane (W)-400610, Maharashtra, India
Thane
MAHARASHTRA
400610
India 
Phone  022-41617181  
Fax  022-41617199  
Email  chandrashekhar.bolmall@biogenomics.co.in  
 
Source of Monetary or Material Support  
BioGenomics Ltd. First Floor, Kothari Compound, Opp. Tikuji-ni-wadi Thane (W) -400601, Maharashtra, India 
 
Primary Sponsor  
Name  BioGenomics Ltd 
Address  BioGenomics Ltd. First Floor, Kothari Compound, Opp. Tikuji-ni-wadi Thane (W) -400601, Maharashtra, India 
Type of Sponsor  Pharmaceutical industry-Indian 
 
Details of Secondary Sponsor  
Name  Address 
NIL  NIL 
 
Countries of Recruitment     India  
Sites of Study  
No of Sites = 1  
Name of Principal Investigator  Name of Site  Site Address  Phone/Fax/Email 
Dr Hiren Prajapati  Veeda Clinical Research   Shivalik Plaza, Near I.I.M, Ambawadi, Ahmedabad 380 015, India
Ahmadabad
GUJARAT 
079300013000
079300013010
hiren.prajapati@veeda.com 
 
Details of Ethics Committee  
No of Ethics Committees= 1  
Name of Committee  Approval Status 
Sangini Hospital Ethics Committee  Approved 
 
Regulatory Clearance Status from DCGI  
Status 
Approved/Obtained 
 
Health Condition / Problems Studied  
Health Type  Condition 
Healthy Human Volunteers  Healthy Human Volunteers 
 
Intervention / Comparator Agent  
Type  Name  Details 
Intervention  G-CSF (BioGenomics Ltd.)  G-CSF 300 mcg/0.5 mL Prefilled Syringe for Subcutaneous route Dose - 0.5 mcg/kg/day once daily for 5 days  
Comparator Agent  Neupogen®   Neupogen® 300 mcg/0.5 mL Prefilled Syringe for Subcutaneous route Dose - 0.5 mcg/kg/day once daily for 5 days 
 
Inclusion Criteria  
Age From  18.00 Year(s)
Age To  45.00 Year(s)
Gender  Male 
Details  Inclusion Criteria
1. Willingness to provide informed consent to participate in the study.
2. Body Mass Index (BMI) between 18.0 kg/m2 to 27.0 kg/m2 (both inclusive) with minimum weight of 50 kg.
3. Nonalcoholic, nonsmoker, healthy, adult, human male volunteers of any race within the age range of 18 to 45 years (both inclusive)living in and around Ahmedabad city or Western part of India.
4. No history of any major illness in the past or absence of clinically significant abnormal findings in physical examination, laboratory evaluations, 12-lead Electrocardiogram (ECG) and X-ray chest Postero-anterior (PA) view during screening.
5. Hemoglobin: ≥12.0 gm%.
6. Subjects without any evidence of impaired glucose tolerance in 2 hour Oral Glucose Tolerance Test (OGTT) at screening.
7. Absence of disease markers of HIV I & II, a negative P24 antigen test, negative Hepatitis B Surface Antigen (HBsAg) and Hepatitis C
Virus Antibody (HCVAb).
8. Comprehension of the nature and purpose of the study and willingness to comply with the requirements of the entire procedure.
9. Negative breath alcohol test at every check-in.
10. Negative urine drug abuse test (barbiturates, benzodiazepines,
opioids, cocaine, cannabinoids and amphetamine, etc.) at every check-in. (This test will be done at the clinical facility).

Exclusion Criteria
1. History / evidence of allergy or hypersensitivity to any drug
2. Any major illness in the last three months or any significant ongoing chronic medical illness
3. Recent history or presence of active kidney or liver dysfunction.
4. Active deep vein thrombosis, pulmonary embolism, arterial thromboembolic disorders or a history of these conditions.
5. History of or current gastrointestinal diseases influencing drug absorption
6. History of drug abuse (including barbiturates, benzodiazepines,
opioids, cocaine, cannabinoids and amphetamine etc.) within last 3 months.
7. History of hyperglycaemia (symptoms include nausea, vomiting,drowsiness, flushed dry skin, dry mouth, increased frequency of urination, thirst and loss of appetite as well as acetone breath).
8. History of diabetic ketoacidosis.
9. History of hypoglycaemia (symptoms include low pulse rate, watering of extremities, dizziness, weakness and sometimes
fainting).
10. A history of neuropsychiatric diseases.
11. Any treatment which could bring about induction or inhibition of hepatic microsomal enzyme system within 1 month of the start of
the study.
12. History or presence of asthma, urticaria or other allergic reactions.
13. History or presence of thyroid disease, adrenal dysfunction, organic intracranial lesion such as pituitary tumour.
14. History or presence of cancer.
15. Consumption of xanthine-containing food and beverages (chocolates, tea, coffee or cola drinks) and tobacco products (Gutka or Pan masala) 48.00 hours prior to check-in.
16. Consumption of grapefruit, grapefruit juice/ products and poppy-containing food and beverages within at least 48.00 hours prior to every check-in.
17. History of difficulty with donating blood or difficulty in accessibility ofveins in left or right arm.
18. Donation of blood (1 unit) within 90 days prior to the first dose of the study drug or have blood loss, excluding volume drawn at screening (more than 100 ml within 30 days; more than 200 ml within 60 days) prior to first dose of the study or have received a known investigational drug within five elimination half-lives of the administered drug prior to the first dose of the study drug.
19. Use of any prescription drug therapy within two weeks and overthe- counter (OTC) drugs or herbal products within one week prior to receiving the dose of study medication and during the study [these include thiazolidinediones, oral hypoglycaemic agents (OHAs), monoamine oxidase inhibitors (MAOIs), non-selective betaadrenergic blocking agents, angiotensin converting enzyme (ACE) inhibitors,salicylates, anabolic steroids (except danazol and oxymetholone), alpha-adrenergic blocking agents, quinine,quinidine, sulphonamides, thiazides, glucocorticoids, thyroid hormones, sympathomimetics, growth hormone, diazoxide, asparaginase, nicotinic acid, oxymetholone, danazol, beta blockers,
octreotide and lanreotide].

 
 
ExclusionCriteria 
Details  1. Allergy or significant history of hypersensitivity or idiosyncratic reaction to E.coli-derived proteins‚ G-CSF or any other excipients in the particular product.
2. Cardiovascular, pulmonary, hepatic, renal, hematological, endocrinal, gastrointestinal, immunologic, dermatologic, neurological or psychiatric disease.
3. Having systolic blood pressure <100 and >130 mmHg, diastolic blood pressure <60 and >88 mmHg and pulse rate <60 and >100
4. Subjects who have been on an unusual diet (for whatever reason, less than 200 kilocalories (kcal) and more than 3000 kcal during the four weeks preceding the study
5. Use of drugs which may potentiate the release of neutrophils‚ such as lithium‚ in the previous 30 days before the study.
6. Female subjects who are currently breast feeding or pregnant or post menopausal (by history or as evidenced by hormonal results)

 
 
Method of Generating Random Sequence    
Method of Concealment    
Blinding/Masking    
Primary Outcome  
Outcome  TimePoints 
To demonstrate equivalence of PK and PD characteristics of G-CSF of Biogenomics and Neupogen® after multiple SC injections in healthy adult human volunteers.  PK-18
PD ANC-16 and CD34-5 
 
Secondary Outcome  
Outcome  TimePoints 
To compare the safety profile and local tolerance of G-CSF of BGL and Neupogen® after multiple SC injections in healthy adult human volunteers  Throughout the study 
 
Target Sample Size   Total Sample Size="64"
Sample Size from India="64" 
Final Enrollment numbers achieved (Total)= "59"
Final Enrollment numbers achieved (India)="59" 
Phase of Trial   Phase 1 
Date of First Enrollment (India)   05/04/2016 
Date of Study Completion (India) 05/07/2016 
Date of First Enrollment (Global)  Date Missing 
Date of Study Completion (Global) Date Missing 
Estimated Duration of Trial   Years="0"
Months="5"
Days="0" 
Recruitment Status of Trial (Global)   Not Applicable 
Recruitment Status of Trial (India)  Completed 
Publication Details   None yet Data still under review. After finalization, we will update the status  
Individual Participant Data (IPD) Sharing Statement

Will individual participant data (IPD) be shared publicly (including data dictionaries)?  

Brief Summary   A phase I, single-centre, randomized, double-blind, two-treatment, two-period, two-sequence, two-way crossover study to demonstrate equivalence of Pharmacokinetic and Pharmacodynamic characteristics and to compare safety and tolerability of Granulocyte Colony Stimulating Factor (BioGenomics Limited) and Neupogen® administered via subcutaneous route as multiple consecutive doses in healthy adult human volunteers 
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