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CTRI Number  CTRI/2026/02/103657 [Registered on: 11/02/2026] Trial Registered Prospectively
Last Modified On: 10/02/2026
Post Graduate Thesis  Yes 
Type of Trial  Interventional 
Type of Study   Other (Specify) [Laser-assisted Laboratory- based procedural intervention]  
Study Design  Randomized, Parallel Group Trial 
Public Title of Study   A Study Comparing Survival of IVF Embryos With and Without Laser Treatment Before Freezing 
Scientific Title of Study   A Prospective Study To Compare The Efectivenes Of Laser-Assisted Blastocyst Collapse Before Vitrification. 
Trial Acronym  nil 
Secondary IDs if Any  
Secondary ID  Identifier 
F.1/IEC/IFS/2026/No.07  Other 
 
Details of Principal Investigator or overall Trial Coordinator (multi-center study)  
Name  Dr KD Nayar 
Designation  Chief Consultant Infertility and IVF 
Affiliation  Akanksha IVF Centre 
Address  Room no.628,mata chanan devi hospital,C-1 Janakpuri New Delhi

West
DELHI
110058
India 
Phone  09810398765  
Fax    
Email  kdnayar@usa.net  
 
Details of Contact Person
Scientific Query  
Name  Gaurav Kant 
Designation  Chief Embryologist 
Affiliation  Akanksha IVF Centre 
Address  Room no.628,mata chanan devi hospital,C-1 Janakpuri New Delhi

West
DELHI
110058
India 
Phone  8750345277  
Fax    
Email  gauravchdry86@gmail.com  
 
Details of Contact Person
Public Query  
Name  Akansha Negi 
Designation  Trainee 
Affiliation  Akanksha IVF Centre 
Address  Room no.628,mata chanan devi hospital,C-1 Janakpuri New Delhi

West
DELHI
110058
India 
Phone  8439284183  
Fax    
Email  negi.akansha0328@gmail.com  
 
Source of Monetary or Material Support  
Akanksha IVF centre, Block C-1 room no.628 mata chanan devi hospital,Janakpuri New Delhi India 110058 
 
Primary Sponsor  
Name  Akanksha Ivf Centre 
Address  Block C-1 room no.628 mata chanan devi hospital,Janakpuri New Delhi India 110058 
Type of Sponsor  Private hospital/clinic 
 
Details of Secondary Sponsor  
Name  Address 
NIL  NIL 
 
Countries of Recruitment     India  
Sites of Study  
No of Sites = 1  
Name of Principal Investigator  Name of Site  Site Address  Phone/Fax/Email 
Akansha Negi  Akanksha IVF centre   Room no.628,mata chanan devi hospital,C-1 Janakpuri New Delhi
West
DELHI 		 08439284183

negi.akansha0328@gmail.com 
 
Details of Ethics Committee  
No of Ethics Committees= 1  
Name of Committee  Approval Status 
Indian Fertility Society  Approved 
 
Regulatory Clearance Status from DCGI  
Status 
Not Applicable 
 
Health Condition / Problems Studied  
Health Type  Condition 
Healthy Human Volunteers  Women with repeated IVF Failures 
 
Intervention / Comparator Agent  
Type  Name  Details 
Intervention  Nil  Nil 
Intervention  Nil  Nil 
 
Inclusion Criteria  
Age From  21.00 Year(s)
Age To  34.00 Year(s)
Gender  Female 
Details  women aged 21 to 34 years undergoing assisted reproductive treatment  
 
ExclusionCriteria 
Details  women aged more than or equal to 35 years at the time of oocyte retrieval 
 
Method of Generating Random Sequence   Not Applicable 
Method of Concealment   Not Applicable 
Blinding/Masking   Not Applicable 
Primary Outcome  
Outcome  TimePoints 
Implantation and Pregnancy rate  Implantation rate: Assessed at 14 days after embryo transfer

Pregnancy rate: Assessed at 6–7 weeks of gestation by ultrasound 
 
Secondary Outcome  
Outcome  TimePoints 
Re-expansion rate of the blastocyst  2–4 hours after warming (post-thaw culture period) 
 
Target Sample Size   Total Sample Size="118"
Sample Size from India="118" 
Final Enrollment numbers achieved (Total)= "Applicable only for Completed/Terminated trials"
Final Enrollment numbers achieved (India)="Applicable only for Completed/Terminated trials" 
Phase of Trial   N/A 
Date of First Enrollment (India)   21/02/2026 
Date of Study Completion (India) Applicable only for Completed/Terminated trials 
Date of First Enrollment (Global)  Date Missing 
Date of Study Completion (Global) Applicable only for Completed/Terminated trials 
Estimated Duration of Trial   Years="0"
Months="3"
Days="0" 
Recruitment Status of Trial (Global)   Not Applicable 
Recruitment Status of Trial (India)  Not Yet Recruiting 
Publication Details   N/A 
Individual Participant Data (IPD) Sharing Statement

Will individual participant data (IPD) be shared publicly (including data dictionaries)?  

Response - YES
  1. What data in particular will be shared?
    Response - All of the individual participant data collected during the trial, after de-identification.

  2. What additional supporting information will be shared?
    Response -  Study Protocol

  3. Who will be able to view these files?
    Response - Anyone

  4. For what types of analyses will this data be available?
    Response - Any purpose.

  5. By what mechanism will data be made available?
    Response - Proposals should be directed to [negi.akansha0328@gmail.com].

  6. For how long will this data be available start date provided 01-05-2026 and end date provided 01-05-2031?
    Response - Immediately following publication. No end date.

  7. Any URL or additional information regarding plan/policy for sharing IPD? 
    Additional Information - nil
Brief Summary  

Blastocyst vitrification is routinely used in in-vitro fertilization (IVF) programs; however, the presence of a fluid-filled blastocoel may influence post-warming survival and subsequent clinical outcomes. Laser-assisted blastocyst collapse is commonly practiced in many laboratories to reduce blastocoelic volume prior to vitrification, but its impact on post-warming recovery and pregnancy outcomes varies across different clinical settings.

This prospective observational study aims to evaluate and compare post-warming survival, re-expansion characteristics, implantation rate, and clinical pregnancy outcomes of blastocysts vitrified with prior laser-assisted collapse and those vitrified without collapse, under routine laboratory conditions. The findings of this study will help assess the effectiveness of laser-assisted blastocyst collapse within the study centre and contribute to evidence-based optimization of blastocyst vitrification practices.

 
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