In this retrospective study approach, data on sub-fertile men visiting the Garbhagudi Institute of Reproductive Health & Research was collected using World Health Organization 2010 criteria. The primary objective of this study is to analyze the relationship of age and lifestyle on DFI of infertile men attending infertility clinic. The secondary objective is to assess the impact of semen parameters and sperm morphology on DFI. To determine whether round head sperms are more common in DFI high level patients’ semen samples.  In this retrospective approach from the IVF center, data of patients are collected based on their chronological age, selected lifestyle characteristics such as alcoholism, smoking, tobacco chewing, BMI and occupational hazard infertile males screened for sperm DFI, and infertility duration of more than two years. Exclusion criteria include patients with retrograde ejaculation, severe oligospermia (under 1 mil/ml), azoospermia, cryopreserved sperm, and surgically extracted sperm. Patients who have incomplete medical records and a history of infertility-related genetic diseases. This study seeks to improve our understanding of how modifiable and non-modifiable factors contribute to sperm DNA damage, potentially aiding in better risk stratification and treatment planning for infertile couples. The findings may also have implications for pre-treatment counselling and lifestyle interventions.