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CTRI Number  CTRI/2025/07/090904 [Registered on: 15/07/2025] Trial Registered Prospectively
Last Modified On: 27/08/2025
Post Graduate Thesis  No 
Type of Trial  PMS 
Type of Study   Nutraceutical 
Study Design  Randomized, Crossover Trial 
Public Title of Study   A Study to Compare the Absorption of a Single Dose of Omega-3 Fatty Acid in Healthy Adults Under Fasting Conditions. 
Scientific Title of Study   Single dose oral comparative bioavailability study of Omega-3 Fatty Acid in healthy adult human subjects under fasting conditions. 
Trial Acronym  NIL 
Secondary IDs if Any  
Secondary ID  Identifier 
OMEG-25-001 Version No. 00  Protocol Number 
 
Details of Principal Investigator or overall Trial Coordinator (multi-center study)  
Name  Dr Shashi Kumar Singh 
Designation  Principal Investigator 
Affiliation  Mediclin Clinical Services Private Limited 
Address  Mediclin Clinical Services Private Limited Plot No. 2A, Main Hardoi Road, Dubagga, Lucknow-226003 (U.P.)

Lucknow
UTTAR PRADESH
226003
India 
Phone  05223502264  
Fax    
Email  shashi.singh@mcscro.in  
 
Details of Contact Person
Scientific Query
 
Name  Dr Kanishk Luhach 
Designation  Manager – Regulatory and Medical Affairs 
Affiliation  Vytals Wellbeing India Private Limited 
Address  Vytals Wellbeing India Private Limited 16th Floor, Block A, DLF Epitome Building 5, DLF Cyber City, DLF Phase 3, Gurugram, Haryana 122002

Gurgaon
HARYANA
122002
India 
Phone  9205789612  
Fax    
Email  kanishk.luhach@vytals.com  
 
Details of Contact Person
Public Query
 
Name  Mr Manoj Karwa 
Designation  Head Clinical Trial & Pharmacovigilance 
Affiliation  Auriga Research Private Limited 
Address  Auriga Research Private Limited 136, IMT Manesar, Haryana, 122050, India

Gurgaon
HARYANA
122050
India 
Phone  9313367656  
Fax    
Email  manojkarwa@aurigaresearch.com  
 
Source of Monetary or Material Support  
Vytals Wellbeing India Private Limited, 16th Floor, Block A, DLF Epitome Building, 5, DLF Cyber City, DLF Phase 3, Gurugram, Haryana 122002 India 
 
Primary Sponsor  
Name  Vytals Wellbeing India Private Limited 
Address  16th Floor, Block A, DLF Epitome Building, 5, DLF Cyber City, DLF Phase 3, Gurugram, Haryana 122002 
Type of Sponsor  Pharmaceutical industry-Indian 
 
Details of Secondary Sponsor  
Name  Address 
Nil  Nil 
 
Countries of Recruitment     India  
Sites of Study  
No of Sites = 1  
Name of Principal Investigator  Name of Site  Site Address  Phone/Fax/Email 
Dr Shashi Kumar Singh  Mediclin Clinical Services Private Limited  Plot No. 2A, Main Hardoi Road, Dubagga, Lucknow-226003 (U.P.)
Lucknow
UTTAR PRADESH 
0522 350 2264

shashi.singh@mcscro.in 
 
Details of Ethics Committee  
No of Ethics Committees= 1  
Name of Committee  Approval Status 
Institutional Human Ethics Committee Udyaan Healthcare  Approved 
 
Regulatory Clearance Status from DCGI  
Status 
Not Applicable 
 
Health Condition / Problems Studied  
Health Type  Condition 
Healthy Human Volunteers  Adult healthy male subjects under fasting condition 
 
Intervention / Comparator Agent  
Type  Name  Details 
Comparator Agent  Plain Fish Oil Softgel Capsule (Each 750mg softgel capsule contain 338mg EPA and 225mg DHA)  As per randomizaion a single dose (4 softgel capsules) of comparator product will be administered in each period with 240 mL of drinking water for swallowing the capsules. 
Intervention  Vytals Fish Oil SMEDs Softgel Capsule (Each 1 g softgel contain 338mg EPA and 225mg DHA)  As per randomizaion a single dose (4 softgel capsules) of intervention product will be administered in each period with 240 mL of drinking water for swallowing the capsules. 
 
Inclusion Criteria  
Age From  18.00 Year(s)
Age To  50.00 Year(s)
Gender  Male 
Details  1.Subject’s age should be between 18 to 50 years (both inclusive).
2. Subjects who are able to read and write the vernacular language (English and Hindi) and understand the study requirements.
3. Must have provided written informed consent for participation in the study in the subject’s vernacular language (English and Hindi).
4. Minimum weight 50.0 kg.
5. BMI 18.50 to 29.50 Kg per m2 (both inclusive).
6. Healthy as determined by medical history, physical, clinical and laboratory examination performed within 28 days prior to admission for the first period of the study.
7. In the opinion of the Principal Investigator or Co Investigator or designee, be able to comply with the study procedures and protocol restrictions. 
 
ExclusionCriteria 
Details  1. Known hypersensitivity (include anaphylaxis, anaphylactic shock, angioedema, bronchospasm, acute interstitial nephritis, and urticaria) or idiosyncratic reaction to (omega 3 fatty acid) or any substituted benzimidazole and its excipients or similar classes of drugs.
2. Any evidence of significant abnormalities upon physical or clinical examination.
3. Laboratory values, which are significantly different from pre defined reference ranges and judged clinically significant.
4. Consumption of grapefruit juice grapefruit at least 48 hours prior to admission in all period of the study.
5. Clinical symptoms of Cyanocobalamin (Vitamin B 12) Deficiency
6. Any clinically significant abnormality in Chest X Ray PA view or invalid Chest X ray PA view (after 01 year of validity).
7. Any clinically significant abnormality in ECG.
8. Regular use of tobacco or nicotine in significant amount in any form (e.g. use of equal to or more than 5 cigarettes a day) or have difficulty in abstaining from (smoking) nicotine use for the entire duration of the study.
9. History of drug dependence or excessive alcohol intake on a habitual basis, or, inability to abstain from alcohol for the entire duration of study.
10. History or presence of significant gastrointestinal, hepatic, renal, cardiovascular, pulmonary, neurological, hematologic, or endocrine disease.
11. History or presence of any chronic illnesses such as arthritis, asthma, epilepsy, hypertension, glaucoma etc.
12. Presence of disease markers of HIV 1 or 2, Hepatitis B or C viruses and syphilis.
13. Positive result for drug(s) of abuse testing (amphetamines, barbiturates, benzodiazepines, tetrahydrocannabinol, morphine, and cocaine) in urine
14. Positive for urine alcohol.
15. History or presence of any psychiatric illness including Symptoms of Depression or mania (Sleep Disturbances, Interest pleasure reduction, Guilt feelings or thoughts of worthlessness, energy changes fatigue, concentration attention impairment, appetite weight changes, psychomotor disturbances and having thoughts of suicide) and or intake of any anti-depressant medications.
16. History or presence of any allergic illness including allergic skin diseases, allergic asthma and drug-induced allergy, e.g., NSAIDs.
17. History of intake administration of any investigational treatment in a clinical study within last 90 days prior to the onset of the study admission of period I of the study
18. History of significant blood loss (more than 350 mL) due to any reason, including blood donation within last 90 days prior to admission of period I of the study.
19. Existence of any surgical or medical condition which in the judgement of principal investigator designee might interfere with the absorption, distribution, metabolism or elimination of the study drug, or, is likely to compromise the safety of subject.
20. Intake or administration of any enzyme modifying drugs such as cimetidine, ampicillin, antihistamines, within 30 days of Period I admission. In such cases, subject selection will be at the discretion of the Principal Investigator or medical officer.
21. Intake or administration of drugs which mentioned below have interaction with Omega 3 fatty acid within last 30 days of Period I admission. In such cases, subject selection will be at the discretion of the Principal Investigator or medical officer.
• Anticoagulants and Antiplatelets (e.g., Warfarin, Aspirin, Clopidogrel, Heparin): May enhance the risk of bleeding due to omega 3 mild antiplatelet effect. Monitoring is required for individuals on blood thinners.
• Antihypertensive Drugs (e.g., ACE inhibitors, Beta blockers, Calcium channel blockers, Diuretics): Omega 3 fatty acids may enhance blood pressure lowering effects, increasing the risk of hypotension.
• Lipid lowering Agents (e.g., Statins, Fibrates, Ezetimibe) May have an additive effect in reducing triglycerides but could also slightly raise LDL cholesterol in some individuals.
• Immunosuppressants (e.g., Cyclosporine, Tacrolimus) High doses of omega 3s may suppress immune function, potentially affecting immunosuppressive therapy.
• Nonsteroidal Anti-Inflammatory Drugs (NSAIDs) (e.g., Ibuprofen, Naproxen, Diclofenac) May increase the risk of bleeding when combined due to their overlapping effects on platelet function.
• Oral Hypoglycemic Agents and Insulin (e.g., Metformin, Sulfonylureas, Insulin) Omega 3 fatty acids may improve insulin sensitivity, which could enhance the glucose lowering effects of these medications, requiring dosage adjustments.
• Hormone Therapy and Oral Contraceptives Estrogen containing contraceptives or hormone replacement therapy may counteract the triglyceride lowering effect of omega 3 fatty acids.
22. Intake or administration of any prescription, antacids or OTC drug including vitamins and natural supplements within 30 days of Period I admission. In such cases, subject selection will be at the discretion of the Principal Investigator or medical officer.
23. Intake of unusual diet for two weeks prior to admission of period I and not willing to avoid consumption of such diet till the completion of close out visit of the study. In such cases, subject selection will be at the discretion of the Principal Investigator or designee.
24. Difficulty in swallowing capsules.
 
 
Method of Generating Random Sequence   Computer generated randomization 
Method of Concealment   Other 
Blinding/Masking   Open Label 
Primary Outcome  
Outcome  TimePoints 
To compare the bioavailability, to characterize the pharmacokinetic profile of the Test Product (T) [Vytals Fish Oil SMEDs Softgel Capsule (Each 1 g softgel contain 338mg EPA and 225mg DHA) of Alder Biochem Private Limited, India] with Reference Product (R) [Plain Fish Oil Softgel Capsule (Each 750mg softgel capsule contain 338mg EPA and 225mg DHA) of Alder Biochem Private Limited, India]  Subjects will visit at study site at period I and period II with the 07 days wash out period and each period Samples will be collected at Pre-dose (0.00) and 0.50, 1.00, 2.00, 4.00, 6.00, 8.00, 10.00, 12.00, 14.00, 16.00, 18.00 and 24.00 hours post dose. 
 
Secondary Outcome  
Outcome  TimePoints 
Safety of the study subjects will also be monitored and evaluated.  Sitting blood pressure, pulse rate, respiratory rate, and body surface temperature will be recorded at check-in and before check-out in each period. Measurements of blood pressure, pulse rate, and body surface temperature will be taken in sitting/supine position at pre-dose (0:00), and at 3, 5, 6, 8, 12, and 24 hours (±40 minutes) post-dose.
AE/SAE monitoring will be conducted throughout the study. 
 
Target Sample Size   Total Sample Size="20"
Sample Size from India="20" 
Final Enrollment numbers achieved (Total)= "Applicable only for Completed/Terminated trials"
Final Enrollment numbers achieved (India)="Applicable only for Completed/Terminated trials" 
Phase of Trial   N/A 
Date of First Enrollment (India)   25/07/2025 
Date of Study Completion (India) Applicable only for Completed/Terminated trials 
Date of First Enrollment (Global)  Date Missing 
Date of Study Completion (Global) Applicable only for Completed/Terminated trials 
Estimated Duration of Trial   Years="0"
Months="3"
Days="0" 
Recruitment Status of Trial (Global)
Modification(s)  
Not Applicable 
Recruitment Status of Trial (India)  Closed to Recruitment of Participants 
Publication Details   N/A 
Individual Participant Data (IPD) Sharing Statement

Will individual participant data (IPD) be shared publicly (including data dictionaries)?  

Response - NO
Brief Summary  
Single dose oral comparative bioavailability study of Omega-3 Fatty Acid in healthy adult human subjects under fasting conditions and the objective of the study is to compare the bioavailability, to characterize the pharmacokinetic profile of the Test Product (T) [Vytals Fish Oil SMEDs Softgel Capsule (Each 1 g softgel contain 338mg EPA and 225mg DHA) of Alder Biochem Private Limited, India] with Reference Product (R) [Plain Fish Oil Softgel Capsule (Each 750mg softgel capsule contain 338mg EPA and 225mg DHA) of Alder Biochem Private Limited, India].

Conducted at a single center, open label, randomized, balanced, two-treatment, two-sequence, two-period, single dose, two-way crossover study involves 20 subjects. Each participant will receive both treatments in a randomized order with a washout period of at least 7 days between doses. Subjects will be housed in the clinical facility at least 12 hours prior to dosing and after an overnight fasting of at least 10 hours dosing will be there. Subjects will continue to remain in the clinical facility for at least 24 hours after dosing in each period. Blood samples will be collected pre-dose and at multiple time points post-dose to assess pharmacokinetic parameters.
 
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