Prediabetes is a state of hyperglycemia with blood sugar levels below the cut-off levels for diabetes. Genetic, environmental, and metabolic risk factors contribute to the development of type 2 diabetes mellitus and an important role of nutritional factors and gut microbiota has been shown in the development of diabetes. Some of the recent studies indicate that changes in gut microbiota can promote insulin resistance and lead to diabetes. Understanding the involvement of gut microbiome in Diabetes Mellitus is a relatively new field and requires further research. Also, probiotics have been shown to improve or alter the gut microbiome as a useful intervention in various pathological states. The present study aims to evaluate the effect of probiotics on glycemic control in prediabetic patients. It will be prospective, parallel group, interventional and open label study of 90 days. It will comprise 70 prediabetic patients of either sex above the age of 18 years from the OPD of Department of Medicine, Guru Nanak Dev Hospital attached to Government Medical College, Amritsar. Patients will be randomly divided into two groups – A and B of 35 patients each. Group A will be prescribed 1 capsule (2.5 billion CFU) of probiotic OD along with diet control. Group B will be put on diet control only. Follow up will be done at day 42 and day 90. Patients will undergo assessment of Fasting plasma glucose, Post prandial plasma glucose, HbA1c, Total lipid profile and any adverse effects during the course of the study. Data will be compiled, tabulated and analyzed statistically.