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CTRI Number  CTRI/2025/08/093239 [Registered on: 19/08/2025] Trial Registered Prospectively
Last Modified On: 19/08/2025
Post Graduate Thesis  Yes 
Type of Trial  Interventional 
Type of Study   Surgical/Anesthesia 
Study Design  Randomized, Parallel Group Trial 
Public Title of Study   Intravenous crystalloid fluid administration after spinal anaesthesia to prevent fall in blood pressure during perioperative management of pregnant patients undergoing elective lower segment caesarean section by randomized control trial. 
Scientific Title of Study   Optimization of crystalloid co-loading speed to stabilize haemodynamics in patient undergoing elective Lower Segment Caesarean section under spinal anaesthesia: A Randomized Control Trial 
Trial Acronym  NIL 
Secondary IDs if Any  
Secondary ID  Identifier 
TP250122323 on 27-07-2025  Protocol Number 
 
Details of Principal Investigator or overall Trial Coordinator (multi-center study)  
Name  DR SATYENDRA YADAV 
Designation  SR CONSULTANT NBEMS AND ASSOCIATE PROFESSOR ANAESTHESIOLOGY 
Affiliation  VEER CHANDRA SINGH GARHWALI GOVERNMENT INSTITUTE OF MEDICAL SCIENCE AND RESEARCH VCSGGIMSR 
Address  OFFICE DEPARTMENT OF ANAESTHESIOLOGY VEER CHANDRA SINGH GARHWALI GOVERNMENT INSTITUTE OF MEDICAL SCIENCE AND RESEARCH VCSGGIMSR SRINAGAR GARHWAL UTTARAKHAND

Garhwal
UTTARANCHAL
246174
India 
Phone  9410110479  
Fax    
Email  satyendra.homein@gmail.com  
 
Details of Contact Person
Scientific Query
 
Name  DR SHUBHAM JOSHI 
Designation  NBEMS DNB ANAESTHESIOLOGY PGJR1 
Affiliation  VEER CHANDRA SINGH GARHWALI GOVERNMENT INSTITUTE OF MEDICAL SCIENCE AND RESEARCH VCSGGIMSR 
Address  OFFICE GUIDE DEPARTMENT OF ANAESTHESIOLOGY VEER CHANDRA SINGH GARHWALI GOVERNMENT INSTITUTE OF MEDICAL SCIENCE AND RESEARCH VCSGGIMSR SRINAGAR GARHWAL UTTARAKHAND

Garhwal
UTTARANCHAL
246174
India 
Phone  9760123604  
Fax    
Email  shubham2016joshi@gmail.com  
 
Details of Contact Person
Public Query
 
Name  DR SATYENDRA YADAV 
Designation  SR CONSULTANT NBEMS AND ASSOCIATE PROFESSOR ANAESTHESIOLOGY 
Affiliation  VEER CHANDRA SINGH GARHWALI GOVERNMENT INSTITUTE OF MEDICAL SCIENCE AND RESEARCH VCSGGIMSR 
Address  OFFICE DEPARTMENT OF ANAESTHESIOLOGY VEER CHANDRA SINGH GARHWALI GOVERNMENT INSTITUTE OF MEDICAL SCIENCE AND RESEARCH VCSGGIMSR SRINAGAR GARHWAL UTTARAKHAND

Garhwal
UTTARANCHAL
246174
India 
Phone  9410110479  
Fax    
Email  satyendra.homein@gmail.com  
 
Source of Monetary or Material Support  
VEER CHANDRA SINGH GARHWALI GOVERNMENT ISTITUTE OF MEDICAL SCIENCE AND RESEARCH, SRIKOT SRINAGAR UTTARAKHAND PIN 246174 
 
Primary Sponsor  
Name  VEER CHANDRA SINGH GARHWALI GOVERNMENT ISTITUTE OF MEDICAL SCIENCE AND RESEARCH 
Address  DEPARTMENT OF ANAESTHESIOLOGY SRIKOT SRINAGAR GARHWAL UTTARAKHAND PIN 246174 
Type of Sponsor  Government medical college 
 
Details of Secondary Sponsor  
Name  Address 
NIL  NIL 
 
Countries of Recruitment     India  
Sites of Study  
No of Sites = 1  
Name of Principal Investigator  Name of Site  Site Address  Phone/Fax/Email 
DR SATYENDRA YADAV  VEER CHANDRA SINGH GARHWALI GOVERNMENT INSTITUTE OF MEDICAL SCIENCE AND RESEARCH  DEPARTMENT OF ANAESTHESIOLOGY AND CRITICAL CARE VCSGGIMS AND RI SRIKOT SRINAGAR GARHWAL UTTARAKHAND PIN 246174
Garhwal
UTTARANCHAL 
9410110479

satyendra.homein@gmail.com 
 
Details of Ethics Committee  
No of Ethics Committees= 1  
Name of Committee  Approval Status 
IEC VCSGGIMS AND R  Approved 
 
Regulatory Clearance Status from DCGI  
Status 
Not Applicable 
 
Health Condition / Problems Studied  
Health Type  Condition 
Patients  (1) ICD-10 Condition: O746||Other complications of spinal andepidural anesthesia during labor and delivery,  
 
Intervention / Comparator Agent  
Type  Name  Details 
Intervention  GROUP A -INTERVENTION 16 GAUGE INTRAVENOUS CANNULA WITH PRESSURE OPTIMIZATION OF CRYSTALLOID CO LOADING.  IN GROUP A OPTIMIZATION OF CRYSTALLOID FLUID SPEED WILL BE DONE BY CONSTANT APPLICATION OF 300MMHG PRESSURE BY PNEUMATIC PRESSURE INFUSER BAG AROUND 500 ML BAG OF RINGER LACTATE. SLEEVE OF PRESSURE INFUSOR BAG WRAPPED AROUND 500 ML BAG OF RINGER LACTATE HANGING ON DRIP STAND AT HEIGHT OF 100 TO 120 CM FROM PATIENT INTRAVENOUS CANNULA END CREATING THE PRESSURE GRADIENT OF 300 MMHG ACROSS CHAMBER SPIKE OF INTRAVENOUS DRIP SET AND TIP OF INTRAVENOUS CANNULA AT PATIENT END.  
Comparator Agent  GROUP B – COMPARATOR 16 GAUGE INTRAVENOUS CANNULA WITH GRAVITY DEPENDENT PASSIVE CRYSTALLOID CO LOADING WITHOUT PRESSURE OPTIMIZATION.  IN GROUP B INTRAVENOUS CRYSTALLOID CO LOADING WILL BE DONE BY GRAVITY DEPENDENT METHOD WITH 500 ML OF RINGER LACTATE BAG ATTACHED ON DRIP STAND AT HEIGHT OF 100-120 CM FROM PATIENT INTRAVENOUS CANNULA END WITHOUT PRESSURE OPTIMIZATION. THE DISTANCE BETWEEN SPIKE OF INTRAVENOUS DRIP SET AND PATIENT INTRAVENOUS CANNULA END IS 100 TO 120 CM. CONVENTIONAL INTRAVENOUS SET OF INTERNAL DIAMETER 3MM AND LENGTH OF 150 CM WILL BE USED FOR INTRAVENOUS FLUID ADMINISTRATION BY 16 GAUGE CANNULA.  
 
Inclusion Criteria  
Age From  20.00 Year(s)
Age To  30.00 Year(s)
Gender  Female 
Details  Pregnant patient aged 20 to 30 years
AMERICAN SOCIETY OF ANAESTHESIOLOGISTS ASA Grade 2
Women with visible vein in dorsum of preferably left hand
Women with no comorbid condition

 
 
ExclusionCriteria 
Details  BMI more than 30 Poorly visible superficial veins on dorsum of hand
Hb less than 10g per dl
Pregnancy induced hypertension
Eclampsia
Foetal distress
Antepartum haemorrhage. Incision to delivery time more than 10 minutes
Height less than 150 cm
Weight less than 40 kg Patient with deranged coagulation profile
Patient with spinal deformity
Patient refusal to participate in the study
 
 
Method of Generating Random Sequence   Coin toss, Lottery, toss of dice, shuffling cards etc 
Method of Concealment   Sequentially numbered, sealed, opaque envelopes 
Blinding/Masking   Participant and Investigator Blinded 
Primary Outcome  
Outcome  TimePoints 
To assess effect of optimization of intravenous crystalloid co loading to prevent maternal hypotension in patient undergoing elective LSCS under spinal anaesthesia.  During perioperative period patient mean arterial pressure, systolic and diastolic blood pressure, heart rate, oxygen saturation and perfusion index will be monitored with ECG NIBP AND PULSE OXIMETRY from baseline to every 2 minutes till 30 minutes after spinal anaesthesia.
Time of 20 ml per kg of crystalloid ringer lactate co loading will be noted in both the groups after spinal anaesthesia during perioperative period. 
 
Secondary Outcome  
Outcome  TimePoints 
To calculate the requirement of vasopressor ephedrine 3mg bolus and anticholinergic drug atropine 0.6 mg during peri-operative period to prevent the maternal hypotension.


 
Time of administration of intravenous ephedrine 6mg bolus will be noted whenever mean arterial pressure falls 20 or more than 20 percent of baseline in spite of crystalloid co loading during perioperative period.
Time of administration of iv injection 0.6 mg bolus whenever the heart falls below 60 bpm after spinal anaesthesia. 
To document the side effects of crystalloid co loading  Side effects such as crepitations in lungs and breathlessness will be noted during any time during perioperative and postoperative period. 
To study the APGAR score of new born  APGAR score will be noted at 1 and 5 minutes after caesarean delivery under spinal anaesthesia. 
 
Target Sample Size   Total Sample Size="68"
Sample Size from India="68" 
Final Enrollment numbers achieved (Total)= "Applicable only for Completed/Terminated trials"
Final Enrollment numbers achieved (India)="Applicable only for Completed/Terminated trials" 
Phase of Trial   N/A 
Date of First Enrollment (India)   30/08/2025 
Date of Study Completion (India) Applicable only for Completed/Terminated trials 
Date of First Enrollment (Global)  Date Missing 
Date of Study Completion (Global) Applicable only for Completed/Terminated trials 
Estimated Duration of Trial   Years="1"
Months="0"
Days="0" 
Recruitment Status of Trial (Global)   Not Yet Recruiting 
Recruitment Status of Trial (India)  Not Yet Recruiting 
Publication Details   N/A 
Individual Participant Data (IPD) Sharing Statement

Will individual participant data (IPD) be shared publicly (including data dictionaries)?  

Response - NO
Brief Summary  


Background

Spinal anaesthesia is established anaesthesia technique for management of elective lower segment caesarean section (LSCS). However post spinal hypotension is common  and may affect maternal and foetal outcomes adversely. Intravenous fluid loading at peak sympathetic blockade (5-7 minutes) is critical to prevent post spinal hypotension. Crystalloid preloading is ineffective and crystalloid co loading though effective and routinely used to prevent post spinal hypotension show contradictory results to mitigate post spinal hypotension  may be due to inadequate crystalloid co loading infusion speed during peak sympathetic block. The optimal speed of crystalloid co loading fluid infusion is under research to prevent post spinal hypotension in obstetric patients undergoing lower segment caesarean section under spinal anaesthesia during perioperative period in this study.

AIM To optimize crystalloid co-loading flow rate/speed to achieve desired co loading volume to stabilize haemodynamic in patient undergoing elective LSCS under spinal anaesthesia to prevent maternal hypotension.


OBJECTIVES

Primary objective -

·       To assess effect of optimizing flow rate/speed of crystalloid co loading to prevent incidence of maternal hypotension in patient undergoing elective LSCS under spinal anaesthesia.

Secondary objective-

·       To calculate the requirement of vasopressor and anticholinergic drug during peri-operative period to prevent the maternal hypotension.

·       To document the side effects of crystalloid co loading.

·       To evaluate the APGAR score of new born.

RATIONALE

Peak sympathetic blockade induced vasodilatation occurs at 5-7 minutes of post spinal anaesthesia. Crystalloid co loading during LSCS with 16 G and 18 G cannula unable to reach in vitro infusion rate in patients at 5-7 minutes, resulted in hypotension. Increasing the pressure gradient across iv infusion bag and tip of catheter by applying constant 300 mmhg of pressure increases the fluid transfusion rate in vitro as per Hagen Poiseuille Equation.  Optimized crystalloid co loading speed and volume by pressure bag (pressurized to 300mmhg constant pressure) at 5-7 minutes likely to prevent post spinal hypotension.

STUDY DESIGN

Prospective, randomized, controlled, double blind, single centre, parallel group comparative clinical trial.

SAMPLE SIZE

The required sample size is determined to be 68 patients with 34 patients in each group.

STUDY DESIGN, MATERIAL AND METHODS

 

STUDY DESIGN  Single Centre Prospective randomized controlled double blind parallel group comparative clinical trial.

STUDY SETTING- Department of Anaesthesiology, VCSGGIMSR Garhwal, Srinagar, Uttarakhand.

STUDY DURATION 10 to 12 months                                                           

After pre-anaesthetic evaluation and informed consent American Society of Anaesthesiologists Grade 2 pregnant patients undergoing elective LSCS under spinal anaesthesia will be taken for study and randomised into two groups by double blind method.

 GROUP A -INTERVENTION 16 Gauge intravenous cannula with optimization of crystalloid co loading speed.

 GROUP BCOMPARATOR 16 Gauge intravenous cannula without optimization of crystalloid co loading speed.

Intravenous cannula   Intravenous cannula of 16 Gauge with flow rate 200 ml per minute will be used in both group A and group B. 

Crystalloid fluid

 Ringer lactate is co loading fluid in dose of 20ml per kg which  will be started in both the groups immediately after giving spinal anaesthesia.

Optimization

In group A optimization of crystalloid fluid flow rate or speed will be done by constant pressure application of 300mmhg by pneumatic pressure infuser bag cuff sleeve wrapped around 500 ml bag of ringer lactate hanging on drip stand creating the pressure gradient across chamber spike of intravenous drip set and tip of intravenous cannula at patient end.

 In group B intravenous crystalloid co loading will be done by gravity dependent method  with 500 ml of ringer lactate bag attached on drip stand without pressure optimization. 

The distance between spike of intravenous drip set and patient intravenous cannula end is 100 to 120 cm in both the groups intravenous ringer lactate co loading will be done by conventional intravenous set of internal diameter 3mm and length of 150 cm.

Randomization will be done by chit box envelope method. Patient cannula hub will be white taped. Blinding or masking will be done by hiding the pressure dial of pressure infusion bag cuff wrapped around 500 ml bag of ringer lactate hanging on drip stand

Spinal anaesthesia will be given using 2 ml hyperbaric 0.5 percent bupivacaine heavy in sitting position in L3  L4 intervertebral space using 25G Quincke’s spinal needle under all aseptic conditions and all the parturient will be placed in supine position with slight inclination.

 The extent of sensory block will be checked every minute using pin-prick method till a sensory block level of T6-T4 is achieved. Co loading with crystalloids (ringer lactate) 20ml per kg will be started just after spinal anaesthesia.

Hypotension will be defined as fall in MAP equal or more than 20% of baseline.

Crystalloid co loading will be started just after giving the spinal anaesthesia with ringer lactate 20 ml per kg. Spike of intravenous drip set at height of 100 to 120 cm from patient cannula end in both the group. In group A constant 300mmhg  pressure will be created with pneumatic pressure sleeve wrapped around the 500 ml ringer lactate bag creating pressure head or gradient between the spike of fluid infusion set and patient intravenous cannula end . This will be done by repeatedly pressing the pump bulb of pneumatic pressure infusion bag by nursing staff or anaesthesia technician or resident doctor not aware of study. In Group B pneumatic pressure infuser pump bulb will be pressed repeatedly with open valve of  pump bulb of pneumatic pressure sleeve wrapped around the 500 ml ringer lactate bag without creating the pressure gradient across chamber spike of intravenous drip set and tip of intravenous cannula at patient end to ensure blinding of study. Investigator will not be aware of group A or B.

Perioperative hypotension will be treated with inj ephedrine-3 mg bolus if there is fall in MAP equal or more than 20 percent of baseline. Total dose of ephedrine will be calculated during perioperative period.

 Bradycardia will be defined as heart rate  less than 60 bpm, intervened by inj atropine 0.6 mg.

Patient observation, monitoring and data recording

Perioperative monitoring tools ECG, NIBP, SPO2 will be used to monitor haemodynamic indices SBP, DBP, MAP, Heart Rate, urine output, perfusion index   noted from basal to the interval of 2 minutes, up to 30 minutes. Induction to incision time, incision to delivery time, duration of surgery, co loading volume 20ml per kg infusion time, requirement of vasopressor- total dose will be noted.

Total fluid volume and time taken to infuse the crystalloid co load will be noted in both the groups. Apgar score will be evaluated at 1 min and 5 min by paediatrician unaware of group allocation.

STATISTICAL ANALYSIS Statistical analysis using unpaired t test and ANOVA test by SPSS OR Open EPI software.



 
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