54 patients undergoing orthodontic treatment will be selected randomly. These patients will be divided into three groups of 18 patients each: Group 1: - Control Group, Group 2: - Probiotic Toothpaste, Group 3: - Probiotic Mouthwash.

Samples will be collected at two different occasions: Before the study begins (Phase 1)(T₀) and After thirty days (Phase 2) (T₁).

Plaque specimens will be collected from the labial surfaces immediately surrounding the orthodontic brackets of the maxillary lateral incisors with a sterilized scaler.

To check the anti-microbial effect, plaque sample collected in container will be inoculated in Luria Broth for isolation of S. mutans. Grown culture will be used for serial dilution, plated on chrome agar plates for specific identification of bacterial growth. The different colour colonies will be further inoculated in Luria Broth and will be incubated overnight at 37°C for growth.  Bacterial isolate will be used for genomic DNA extraction, G DNA will be isolated using Genomic DNA extraction kit, followed by 16srdna using universal primers for 16srDNA, PCR samples will be confirmed on (0.8%) agarose gel electrophoresis. PCR sample will be purified using PCR clean up kit. PCR cleanup product will be sent for sequencing.  Protocol used for PCR amplification will be followed accordingly. Thermocycle conditions are 94°C 3 minutes, 94°C 45 seconds, 50°C 60 seconds, 72°C 90 seconds, Repeat steps 2-4 35 times, 72°C 10 minutes, 4°C HOLD.

For Results – Colony Forming Units (CFU) will be counted.