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CTRI Number  CTRI/2024/12/078394 [Registered on: 20/12/2024] Trial Registered Prospectively
Last Modified On: 02/12/2025
Post Graduate Thesis  No 
Type of Trial  Interventional 
Type of Study   Drug 
Study Design  Randomized, Parallel Group, Active Controlled Trial 
Public Title of Study   A clinical study to assess the efficacy and safety of Ademetionine Injection in patients with bile flow problems inside the liver. 
Scientific Title of Study   A Phase III, Randomized, Open Label, Active Controlled, Prospective, Parallel Group, Comparative, Multicentric Clinical Study to Evaluate the Efficacy, Safety and Tolerability of Ademetionine 1,4-Butane Disulfonate 500 mg Lyophilized Powder for Injection in Adult Patients with Intrahepatic Cholestasis (IHC). 
Trial Acronym  NIL 
Secondary IDs if Any  
Secondary ID  Identifier 
CT/2024/26, Version No.: 00 and Dated May 01, 2024  Protocol Number 
 
Details of Principal Investigator or overall Trial Coordinator (multi-center study)  
Name  Dr Rajasekhara Reddy Tamma 
Designation  Managing Director 
Affiliation  Clinwave Research Pvt. Ltd. 
Address  LIG: B/466, H. No.: 1-16-10/466, Dr. A.S. Rao Nagar, Kapra, Medchal-Malkajgiri (Dist.).

Medchal
TELANGANA
500062
India 
Phone  7989233379  
Fax    
Email  dr.sekhar@clinwave.co.in  
 
Details of Contact Person
Scientific Query
 
Name  Dr Harsh Shah 
Designation  AGM - Medical Affair & Pharmacovigilance 
Affiliation  La Renon Healthcare Pvt. Ltd. 
Address  207-208, ISCON Elegance, Circle P, Prahaldnagar Crossroads, SG Highway.

Ahmadabad
GUJARAT
380015
India 
Phone  9879540270  
Fax    
Email  harsh.shah@larenon.com  
 
Details of Contact Person
Public Query
 
Name  Dr Harsh Shah 
Designation  AGM - Medical Affair & Pharmacovigilance 
Affiliation  La Renon Healthcare Pvt. Ltd. 
Address  207-208, ISCON Elegance, Circle P, Prahaldnagar Crossroads, SG Highway.

Ahmadabad
GUJARAT
380015
India 
Phone  9879540270  
Fax    
Email  harsh.shah@larenon.com  
 
Source of Monetary or Material Support  
La Renon Healthcare Pvt. Ltd., 207-208, ISCON Elegance, Circle P, Prahaldnagar Crossroads, SG Highway, Ahmedabad-380015, Gujarat, India. 
 
Primary Sponsor  
Name  La Renon Healthcare Pvt. Ltd. 
Address  207-208, ISCON Elegance, Circle P, Prahaldnagar Crossroads, SG Highway, Ahmedabad-380015, Gujarat, India. 
Type of Sponsor  Pharmaceutical industry-Indian 
 
Details of Secondary Sponsor  
Name  Address 
NIL  NIL 
 
Countries of Recruitment     India  
Sites of Study
Modification(s)  
No of Sites = 10  
Name of Principal Investigator  Name of Site  Site Address  Phone/Fax/Email 
Dr Sagorika Mullick  Abhayahasta Multispeciality Hospital  Research Room, 347/247, 2nd Cross, Kaggadasapura Main Road, Landmark Opp. to DARE, CV Raman Nagar, Bengaluru-560093.
Bangalore
KARNATAKA 
9653433664

cr.abhayahasta@gmail.com 
Dr Mahavir Prasad Sharma  Jawahar Lal Nehru (J.L.N) Medical College  Department of Gastroenterology, Kala Bagh, Ajmer-305001.
Ajmer
RAJASTHAN 
7737258659

doctor.mpsharma@outlook.com 
Dr Renuka Rayana  King George Hospital  Department of General Medicine, Rajendra Prasad Ward, Maharanipeta, Visakhapatnam-530002.
Visakhapatnam
ANDHRA PRADESH 
9030526346

drrrenukaresearch@gmail.com 
Dr Subhash Chander  Maharaja Agrasen Superspeciality Hospital  Research Room, Central Spine, Agrasen Aspatal Marg, Sector 7, Vidyadhar Nagar, Jaipur-302039.
Jaipur
RAJASTHAN 
9828511073

dr.subhashchander@outlook.com 
Dr Shyam Sunder Sharma  Manglam Plus Medicity Hospital  Research Room, Sector 05, Shipra Path, Mansarovar, Jaipur-302020.
Jaipur
RAJASTHAN 
7737258659

mmedcityresearch@gmail.com 
Dr Raja Bhattacharya  Medical College and Hospital, Kolkata  Department of Medicine, MCH Building, 4th Floor, 88 College Street, Kolkata-700073.
Kolkata
WEST BENGAL 
8100273048

rbrbhattacharya@gmail.com 
Dr Nikhil Jayprakash Jillawar  Ojas Multispeciality Hospital  Research Room, Sr. No. 203/01, Bhondave Chowk, D.Y Patil College Road, Ravet, Pune- 412101.
Pune
MAHARASHTRA 
9099058141

dr.nikhilojas@gmail.com 
Dr Veer Bahadur Singh  Sardar Patel Medical College & A.G. Hospitals  Department of Medicine, Sardar Patel Colony, Bikaner-334001.
Bikaner
RAJASTHAN 
9414136888

vbsingh2@rediffmail.com 
Dr Manoj Lahoti  Suyash Institute of Medical Science Pvt. Ltd.  Gastroenterology OPD, Unit-2, Gudhiyari Road, Kota, Raipur-492001.
Raipur
CHHATTISGARH 
9826851120

manojlahoti24@gmail.com 
Dr Make Naveen Chad  Visakha Institute of Medical Sciences  Department of Gastroenterology, NH-16, Hanumanthavaka Junction, Visakhapatnam-530040.
Visakhapatnam
ANDHRA PRADESH 
9885837772

drmnaveenchandresearch@gmail.com 
 
Details of Ethics Committee
Modification(s)  
No of Ethics Committees= 10  
Name of Committee  Approval Status 
Ethics Committee, S.P. Medical College  Approved 
Institutional Ethics Committee for Human Research, Medical College and Hospital, Kolkata.  Approved 
Institutional Ethics Committee, Abhayahasta Superspeciality Hospital  Approved 
Institutional Ethics Committee, Jawahar Lal Nehru Medical College  Approved 
Institutional Ethics Committee, King George Hospital  Approved 
Institutional Ethics Committee, Maharaja Agrasen Superspeciality Hospital  Approved 
Institutional Ethics Committee, Manglam Medicity Hospital  Approved 
Institutional Ethics Committee, Visakha Institute of Medical Sciences (VIMS)  Approved 
Ojas Multispeciality Hospital Ethics Committee  Approved 
Suyash Hospital Institutional Ethics Committee  Approved 
 
Regulatory Clearance Status from DCGI  
Status 
Approved/Obtained 
 
Health Condition / Problems Studied  
Health Type  Condition 
Patients  (1) ICD-10 Condition: K769||Liver disease, unspecified,  
 
Intervention / Comparator Agent  
Type  Name  Details 
Intervention  Ademetionine 1,4-Butane Disulfonate 500 mg Lyophilized Powder for Injection  The recommended dosing is 5-12 mg/kg/day IV or IM. The usual starting dose is 500 mg/day IV or IM for 2 weeks.  
Comparator Agent  Ademetionine-1,4-Butanedisulfonate Tablets 400 mg  one tablet thrice a day orally, swallowed with water in the morning, afternoon and evening around same time every day for 2 weeks. 
 
Inclusion Criteria  
Age From  18.00 Year(s)
Age To  65.00 Year(s)
Gender  Both 
Details  1. Male and female patients aged between 18 to 65 years (both inclusive).
2. Patients with diagnosis of intrahepatic cholestasis (IHC):
Serum total bilirubin and serum conjugated bilirubin levels more than 2 times upper limit of normal (ULN).
Alkaline phosphatase (ALP) and / or Gamma glutamyl transferase (GGT) more than 2 times upper limit of normal (ULN).
3. Women of childbearing potential (WOCBP) must be using an acceptable method of contraception to avoid pregnancy throughout the study. WOCBP must have a negative urine pregnancy test at screening / baseline visit.
4. Patient with ability to understand and provide written, signed and dated informed consent form, which must have been obtained prior to screening.
5. Patients willing to comply with the protocol requirements. 
 
ExclusionCriteria 
Details  1. Patients with a known hypersensitivity to the active substance of Ademetionine.
2. Patients with a history of consumption of steatogenic medications in the 6 months prior to screening, such as Amiodarone, Methotrexate, Tamoxifen, Valproate, anti-retroviral medicine, NSAIDs, statins, neuroleptics, anti-convulsants, corticosteroids, etc. as per available records.
3. Patients with a history of other causes of chronic liver disease (NAFLD, autoimmune liver diseases, viral hepatitis (Hepatitis B virus and Hepatitis C virus), Wilson’s disease, hemochromatosis) and metabolic syndrome.
4. Patients with a history of severe liver disease such as with Child-Pugh Class B or C and with any end-stage liver disease.
5. Patients with known or history of genetic defects affecting the methionine cycle and/or causing homocystinuria and/or hyperhomocysteinemia (e.g., cystathionine beta-synthase deficiency, Vitamin B12 metabolism defect) or known folate, Vitamin B6 or B12 deficiency).
6. Patients with renal dysfunction (serum creatinine ≥ 2.5 mg/dL).
7. Patients with history of liver transplantation.
8. Patients with a history of active heavy alcohol intake (Greater than 150 g/day).
9. Patients who require ICU setting management.
10. Patients on any treatment with other drugs claimed for treatment of alcoholic liver disease (i.e., Pentoxyphylline, steroids, Ursodeoxycholic Acid, acetyl cholinesterase enzyme inhibitors antioxidants such as Vitamin E, Vitamin C, GSH, alpha-tocopherol, or non-prescribed complementary alternative medications [including dietary supplements]).
11. Patients with extrahepatic cause of cholestasis (proven by ultrasound or described in medical history).
12. Patients with a history of active substance abuse (oral, inhaled or injected) within one year prior to the study.
13. Patients on total parenteral nutrition in the year prior to screening.
14. Patients after or planned for bariatric surgery (jejunoileal bypass or gastric weight loss surgery).
15. Patients with any of the following disease in medical history:
Evidence of autoimmune liver disease
Wilson’s disease
Hemochromatosis
Alpha-1-antitrypsin deficiency
16. Patients with a history of biliary diversion.
17. Patients with a history of major depression or bipolar disease.
18. Patients with a known case of clinically significant cerebrovascular disease, cardiovascular disease, thyroid dysfunction, chronic uncontrolled systemic diseases like asthma, hypertension, collagen disorders, severe infections, that may affect patient safety.
19. Patients with any abnormality on 12-lead ECG at screening that in the opinion of the investigator is clinically significant and is judged as potential risk for patient’s participation in the study.
20. Female patients who are pregnant or breast-feeding or expecting to conceive within the projected duration of the study.
21. Female patients who are of childbearing potential and who are neither surgically sterilized nor willing to use reliable contraceptive methods (like hormonal, barrier methods or intrauterine device).
22. Patients with a history of any malignancy.
23. Patients with history of ascites, hepatic encephalopathy, varices or bleeding.
24. Patients with concurrent participation in another clinical trial or any investigational therapy within 30 days prior to signing informed consent.
25. Patients currently taking any of the prohibited medications(s) and inability/unwillingness to discontinue them for the entire study period.
26. Patients with suspected inability or unwillingness to comply with the study procedures.
27. Patient with any condition which, in the judgment of the Investigator, may render the patient unable to complete the study or which may pose a significant risk to the patient. 
 
Method of Generating Random Sequence   Computer generated randomization 
Method of Concealment   Not Applicable 
Blinding/Masking   Open Label 
Primary Outcome  
Outcome  TimePoints 
Mean change in serum total bilirubin and serum conjugated bilirubin from baseline to end of the study visit (week 2).  Visit 1 - Screening or Baseline visit (Day -3),
Visit 3 - Follow up visit / week 1 (Day 8±1) and
Visit 4 - End of the study visit / Week 2 (Day 15±2). 
 
Secondary Outcome  
Outcome  TimePoints 
Mean change in alkaline phosphatase (ALP) from baseline to end of the study visit (week 2).  Visit 1 - Screening or Baseline visit (Day -3),
Visit 3 - Follow up visit / week 1 (Day 8±1) and
Visit 4 - End of the study visit / Week 2 (Day 15±2).
 
Mean change in gamma glutamyl transferase (GGT) from baseline to end of the study visit (week 2).  Visit 1 - Screening or Baseline visit (Day -3),
Visit 3 - Follow up visit / week 1 (Day 8±1) and
Visit 4 - End of the study visit / Week 2 (Day 15±2). 
Mean change in hepatic transaminases (i.e., SGOT and SGPT) from baseline to end of the study visit (week 2).  Visit 1 - Screening or Baseline visit (Day -3),
Visit 3 - Follow up visit / week 1 (Day 8±1) and
Visit 4 - End of the study visit / Week 2 (Day 15±2). 
Changes in clinical symptoms of cholestasis (pruritus, fatigue and jaundice) as assessed by the investigator from baseline to end of the study visit (week 2).  Visit 1 - Screening or Baseline visit (Day -3),
Visit 3 - Follow up visit / week 1 (Day 8±1) and
Visit 4 - End of the study visit / Week 2 (Day 15±2). 
Adverse events or Serious adverse events reported during the study.  Throughout the study 
Changes in clinical laboratory parameters from baseline to end of the study visit (week 2).  Visit 1 - Screening or Baseline visit (Day -3) and
Visit 4 - End of the study visit / Week 2 (Day 15±2). 
 
Target Sample Size   Total Sample Size="180"
Sample Size from India="180" 
Final Enrollment numbers achieved (Total)= "180"
Final Enrollment numbers achieved (India)="180" 
Phase of Trial   Phase 3 
Date of First Enrollment (India)   06/01/2025 
Date of Study Completion (India) 16/10/2025 
Date of First Enrollment (Global)  Date Missing 
Date of Study Completion (Global) Date Missing 
Estimated Duration of Trial   Years="1"
Months="0"
Days="0" 
Recruitment Status of Trial (Global)   Not Applicable 
Recruitment Status of Trial (India)  Completed 
Publication Details   N/A 
Individual Participant Data (IPD) Sharing Statement

Will individual participant data (IPD) be shared publicly (including data dictionaries)?  

Response - NO
Brief Summary  

This trial is a phase III, randomized, open label, active controlled, prospective, parallel group, comparative, multicentric clinical study to evaluate the efficacy, safety and tolerability of Ademetionine 1,4-Butane Disulfonate 500 mg Lyophilized Powder for Injection in adult patients with intrahepatic cholestasis (IHC).

Patients who are willing and able to participate in the study will sign and date the Informed Consent Form on the day of screening or baseline visit (Visit 1). During this screening period, patients who are willing to give consent will be evaluated for all the eligibility criteria. Eligible patients (male and female) aged between 18 to 65 years (both inclusive), with a diagnosis of intrahepatic cholestasis (IHC) [Serum total bilirubin and conjugated bilirubin levels more than 2 times upper limit of normal (ULN) AND Alkaline phosphatase (ALP) and / or Gamma glutamyl transferase (GGT) more than 2 times upper limit of normal (ULN)] at screening visit will be considered for the study.

After confirming the inclusion/exclusion criteria the subject will be randomized and provided with study drug at randomization visit. Subjects will be provided with patient diary at randomization visit, which need to be brought along with in each subsequent visit till the last visit. Follow up visits will be done on week 1/day 8(±1) and week 2/day 15(±2) (Final Visit) of treatment to assess efficacy, safety and tolerability.

Patients will be assigned to either of the two arms i.e., Arm A or Arm B consisting of Ademetionine 1,4-Butane Disulfonate 500 mg Lyophilized Powder for Injection (Arm A) or Ademetionine-1,4-Butanedisulfonate Tablets 400 mg (Arm B).


 
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