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CTRI Number  CTRI/2015/05/005737 [Registered on: 05/05/2015] Trial Registered Prospectively
Last Modified On: 27/04/2016
Post Graduate Thesis  No 
Type of Trial  BA/BE 
Type of Study    
Study Design  Randomized, Crossover Trial 
Public Title of Study   To compare bioavailibility of Methotrexate Tablets 2.5 mg( Actavis LLC) with Methotrexate Tablets USP 2.5 mg (DAVA Pharmaceuticals, Inc.USA) in patients with mild to severe psoriasis or rheumatoid arthritis, who are already on established regimens of 2.5 mg every 12 hours under fasting condition.  
Scientific Title of Study   A multicenter, randomized, open label, two treatment, two period, two sequence, single-dose, crossover, bioequivalence study of Methotrexate Tablets 2.5 mg (manufactured for Actavis LLC) with Methotrexate Tablets USP 2.5 mg (DAVA Pharmaceuticals, Inc., Fort Lee, NJ 07024 USA) in patients with mild to severe psoriasis or rheumatoid arthritis (RA), who are already on established regimens of 2.5 mg every 12 hours under fasting condition. 
Trial Acronym  NA 
Secondary IDs if Any  
Secondary ID  Identifier 
14-VIN-721 Version 01 dated 17 Dec 2014  Protocol Number 
 
Details of Principal Investigator or overall Trial Coordinator (multi-center study)
Modification(s)  
Name  Dr Ashoka Kumar Singh 
Designation  Head of Department  
Affiliation  Veeda Clinical Research Pvt. Ltd.  
Address  Veeda Clinical Research Pvt. Ltd.Insignia, Sindhu Bhavan Road, Bodakdev Road, S.G.highway. Ahmedabad Ahmadabad GUJARAT

Ahmadabad
GUJARAT
380059
India 
Phone  07930013001  
Fax    
Email  Ashoka.Singh@veedacr.com  
 
Details of Contact Person
Scientific Query

Modification(s)  
Name  Dr Ashoka Kumar Singh 
Designation  Head of Department  
Affiliation  Veeda Clinical Research Pvt. Ltd.  
Address  Veeda Clinical Research Pvt. Ltd.Insignia, Sindhu Bhavan Road, Bodakdev Road, S.G.highway. Ahmedabad Ahmadabad GUJARAT

Ahmadabad
GUJARAT
380059
India 
Phone  07930013001  
Fax    
Email  Ashoka.Singh@veedacr.com  
 
Details of Contact Person
Public Query

Modification(s)  
Name  Dr Ashoka Kumar Singh 
Designation  Head of Department  
Affiliation  Veeda Clinical Research Pvt. Ltd.  
Address  Veeda Clinical Research Pvt. Ltd.Insignia, Sindhu Bhavan Road, Bodakdev Road, S.G.highway. Ahmedabad Ahmadabad GUJARAT

Ahmadabad
GUJARAT
380059
India 
Phone  07930013001  
Fax    
Email  Ashoka.Singh@veedacr.com  
 
Source of Monetary or Material Support  
Actavis LLC 
 
Primary Sponsor  
Name  Actavis LLC 
Address  Actavis LLC – Morris Corporate Center III,400 Interpace Parkway Parsippany, NJ 07054 
Type of Sponsor  Pharmaceutical industry-Global 
 
Details of Secondary Sponsor  
Name  Address 
NIL  NIL 
 
Countries of Recruitment     India  
Sites of Study  
No of Sites = 16  
Name of Principal Investigator  Name of Site  Site Address  Phone/Fax/Email 
Dr Siva G Prasad  Apollo Hospitals  Waltair main road, Vizag -530002
Visakhapatnam
ANDHRA PRADESH 
09441606666
089125608558
dr_sivaji@yahoo.co.in 
Dr Kalpesh Mehta  B. J. Medical collage and Civil Hospital  Orthopedic Department, Asarwa, Ahmedabad-380016, Gujarat
Ahmadabad
GUJARAT 
09879517351

drkaps@rediffmail.com 
Dr Sharmila Patil  D. Y. Patil Hospital  Department of dermatology, OPD No.: 54, 1st floor Nerul, New mumbai-400706
Mumbai
MAHARASHTRA 
09821350217
39216063
drsharmilapatil@gmail.com 
Dr Mukesh Sancheti  Ganga CARE Hospital Limited  Department Of Orthopedics, 3 Farmland Panchsheel square, Ramdaspeth, Nagpur-440012
Nagpur
MAHARASHTRA 
09823158625
07122420158
mksancheti@gmail.com 
DrSomesh Singh  GMERS Medical Collage & Civil Hospital Sola  Department Of Orthopedics, Near Gujarat High Court, SG highway, Sola,Ahmedabad-380061
Ahmadabad
GUJARAT 
09825370446

drsomeshsingh@yahoo.com 
Dr Sajal Mitra   Govt. Medical College & Hospital  Department of Orthopedic, Medical Square, Nagpur-440003
Nagpur
MAHARASHTRA 
09373114929
0712-2744489
sajalmitra2007@yahoo.co.in 
Dr Pradyumna Vaidya  Jahangir Clinical Development Centre Pvt. Ltd  Jahangir hospital Premises, 32 Sasoon road, Pune 411001
Pune
MAHARASHTRA 
09822400964
02026059319
drpvaidya@gmail.com 
Dr M Pradha Saradhi  King George Hospital  Department Of Orthopedics, King George Hospital, Visakhapatnam-530002, A.P. India
Visakhapatnam
ANDHRA PRADESH 
09440608752
891-2704848
amcorthopaedics@hotmail.com 
Dr Laxmikant Goyal  Malpani Multi Speciality Hospital  orthopedic OPD,SP-6, Road No. 1, V.K.I. Are, Sikar road, Jaipur-302013
Jaipur
RAJASTHAN 
09462651019
01412333886
drlkgoyal@gmail.com 
Dr Harish Mathur  Mavens Hospital  Department Of Orthopedics, N-37, Vaishalinagar, Ajmer, Rajasthan-305001
Ajmer
RAJASTHAN 
09829279052

drharishmathur@yahoo.com 
Dr Sushil Haridas Mankar  N K P Salve Institute of Medical Sciences & Lata Mangeshkar Hospital   Orthopedic department, Digdoh Hills Hingna road, Nagpur-440019
Nagpur
MAHARASHTRA 
09422108284
07104-306100
drshmankar@rediffmail.com 
DrTSudheer  Rajiv Gandhi Institute of Medical Sciences & RIMS Government General Hospital,  Department of Orthopedics, Research wing 2nd floor, Beside FM ward. Srikakulam-532001
Srikakulam
ANDHRA PRADESH 
09949900369
8942-279033
rimsresearch@gmail.com 
Dr Gaurav Rathi  Rathi Hospital  orthopedic OPD, Near Anupam Shopping Centre, Opp. Mahabaleswar society, Nr. Jodhpur cross road, Satelite, Ahmedabad-380015
Ahmadabad
GUJARAT 
09824015618
07926924812
drgjrathi@yahoo.co.in 
Dr Ruchir Shah  Sanjivani Superspeciality Hospital Pvt. Ltd.  Orthopedic OPD, 1 Uday Park Society, Nr.Sunrise park, Vastrapur, Ahmedabad-380015
Ahmadabad
GUJARAT 
09904079691
07926300411
drruchirshah.dermatologist@gmail.com 
Dr Ravindra Wadhawan  Saviour Multispecialty Hospital  Orthopedic OPD, Nr Bharat Petroleum, Nr.Lakhudi Talav, Stadium road, Navrangpura, Ahmedabad-380014
Ahmadabad
GUJARAT 
09825010673
07961908099
raviwadhawan2000@yahoo.com 
Dr Harshal Kalaria  Shree Giriraj Multispeciality Hospital  27 Navjyot Park Corner, 150 Feet ring road, Rajkot-360005
Rajkot
GUJARAT 
09979883331
02812587222
harshalkalaria@gmail.com 
 
Details of Ethics Committee
Modification(s)  
No of Ethics Committees= 16  
Name of Committee  Approval Status 
Care Hospital Ethics Committee  Submittted/Under Review 
Ethics Committee Apollo Hospital  Submittted/Under Review 
Ethics Committee Jahangir Clinical Development Centre Pvt. Ltd.  Submittted/Under Review 
Ethics Committee Rajiv Gandhi Institute of Medical Sciences & RIMS Government General Hospital  Approved 
Institutional Ethics Committee B.J. Medical College & Civil Hospital  Submittted/Under Review 
Institutional Ethics Committee Department of Pharmacology Govt Medical College  Submittted/Under Review 
Institutional Ethics Committee G.M.E.R.S. Medical College  Approved 
Institutional Ethics Committee King George Hospital  Approved 
Institutional Ethics Committee Malpani Multispeciality Hospital  Approved 
Institutional Ethics Committee NKP Salve Institute of Medical Sciences & Lata Mangeshkar Hospital  Submittted/Under Review 
Institutional Ethics committee Padamshree Dr. D.Y. Patil Medical College & Hospital and Research Centre  Submittted/Under Review 
MAVENS Institutional Ethics Committee  Approved 
Rathi Ethics Committee  Approved 
Sanjivani Hospital Ethics Committee  Approved 
Saviour Hospital Ethics Committee  Approved 
Shree Giriraj Hospital Research Ethics Committee  Approved 
 
Regulatory Clearance Status from DCGI  
Status 
No Objection Certificate 
 
Health Condition / Problems Studied  
Health Type  Condition 
Patients  mild to severe psoriasis or rheumatoid arthritis ,  
 
Intervention / Comparator Agent  
Type  Name  Details 
Intervention  Methotrexate Tablets 2.5 mg (Actavis LLC))  On day 1 of each period, each patient would receive their dose of Methotrexate 2.5 using either the test or reference product in a crossover design. Two subsequent doses will be of locally approved drug at every 12 hrs of interval after the administration of study drug on day 1. 
Comparator Agent  Methotrexate Tablets USP 2.5 mg (DAVA Pharmaceuticals )  On day 1 of each period, each patient would receive their dose of Methotrexate 2.5 using either the test or reference product in a crossover design. Two subsequent doses will be of locally approved drug at every 12 hrs of interval after the administration of study drug on day 1. 
 
Inclusion Criteria  
Age From  18.00 Year(s)
Age To  65.00 Year(s)
Gender  Both 
Details  1. Men or Women, in between 18 to 65 years of age (both inclusive).
2.Ability to provide informed consent prior to participation in the study
3. Patients with mild to severe psoriasis or rheumatoid arthritis, who are already on established regimens of 2.5 mgevery 12 hours (7.5 mg per week in three divided doses).
4.Confirmed diagnosis of psoriasis by clinical examination.or Confirmed diagnosis of mild to severe rheumatoid arthritis based on at least 1 of the following:
i. Documented history of positive rheumatoid factor
ii. Current presence of rheumatoid factor
iii. Radiographic erosion within 12 months prior to enrolment
iv. Presence of serum anti-cyclic citrullinated peptideantibodies (anti-CCP).
5. Women of childbearing potential must have a negative serum or urine pregnancy test, must be using an adequate method of contraception.
6.Patient’s screening laboratory assessment (complete blood count and blood chemistries) are clinically nonsignificant as per the discretion of the Investigator.
7. No history of addiction to any recreational drug or drug dependence.
8. No participation in any clinical study within the past 60 days. 
 
ExclusionCriteria 
Details  1.A history of allergic or adverse reactions to Methotraxate Sodium or any comparable or similar product
2. Patients with alcoholism, alcoholic liver disease or other chronic liver disease.
3.Patients who have overt or laboratory evidence of immunodeficiency syndromes.
4.Patients who have pre-existing blood dyscrasias, such as bone marrow hypoplasia, leukopenia, thrombocytopenia or significant anemia
5. Expected changes in concomitant medications during the period of study
6.Tested positive for Alcohol breath or Urine drug of abuse.
7.Patients who are:pregnant, breast feeding,Of childbearing potential without a negative pregnancy test at baseline, Male or female of childbearing potential unwilling to use barrier contraceptive precautions throughout the trial and at least for 3 months (for males) and for at least one ovulatory cycle (for females) after last dose of study medication, Patient had major surgery within 4 weeks prior to study entry, or who have not recovered from prior major surgery,Patients with known positivity for human
immunodeficiency virus (HIV), HBsAg and HCV, Patients with any significant history of non-compliance to medical regimens or with inability to grant a reliable informed consent.
8.History of difficulty with donating blood or difficulty in accessibility of veins.
9. Oral Administration of Drug is not possible.
10.An unusual or abnormal diet, for whatever reason e.g.religious fasting.
11.Donation of blood (1 unit or 350 ml) within 90 days prior to receiving the first dose of investigational medicinal product for the current study.
12.Evidence of any significant uncontrolled concomitant disease which in the investigators opinion would exclude the patient participation.
13. Patients with any evidence of organ dysfunction or any clinically significant deviation from normal in their physical or clinical evaluation including ECG and X-ray results except study indication.
14. Any treatment which could affect the pharmacokinetic of methotrexate (salicylates, hypoglycaemics, diuretics,sulphonamides, diphenylhydantoins, tetracyclines,chloramphenicol and p-aminobenzoic acid, probenecid,penicillins, Chloroquine, omeprazole, etretinate, cotrimoxazole and trimethoprim etc.) administered within 1 month of starting of study.
15.Patients who are diagnosed to be HIV 1 and 2 or Hepatitis B(HBsAg) or Hepatitis C (HCV) virus reactive/positive.
16. Patients with clinically significant abnormal haemoglobin(Hb), total white blood cells count (WBC), differential WBC count, platelet count and hematocrite.
17. Patients who, have clinically significant abnormal laboratory values.
18.Patients with a clinically significant past history or current medical condition of:Pulmonary disorders (COPD and asthma),Cardiovascular disorders (especially cardiac blocks), Neurological disorders, GIT disorders including history or presence of significant gastric and duodenal ulceration, Renal and/or hepatic disorder
coagulation disorders.
19.Endocrine disorders (especially diabetes mellitus).
20.History or presence of cancer. 
 
Method of Generating Random Sequence   Computer generated randomization 
Method of Concealment   Not Applicable 
Blinding/Masking   Open Label 
Primary Outcome  
Outcome  TimePoints 
The objective of this pivotal study is to compare the
bioavailability of methotrexate from Methotrexate Tablets 2.5
mg (manufactured for Actavis LLC) and Methotrexate Tablets
USP 2.5 mg (DAVA Pharmaceuticals,USA) in patients with mild to severe psoriasis or rheumatoid
arthritis (RA), who are already on established regimens of 2.5 mg every 12 hours under fasting condition. 
predose sample of 3.5 mL will be collected within 5 minutes before dosing on day 1 of each period. Post dose sample of 3.5 mL each will be drawn at 0.167,0.250,0.500,0.750,1.000,1.250,1.500,1.750,2.000,2.333,2.667, 3.000, 3.500, 4.000, 4.500, 5.000, 6.000, 7.000, 8.000, 10.000, & 12.000 
 
Secondary Outcome  
Outcome  TimePoints 
To monitor the adverse events and to ensure the safety of Patient  NA 
 
Target Sample Size   Total Sample Size="42"
Sample Size from India="42" 
Final Enrollment numbers achieved (Total)= ""
Final Enrollment numbers achieved (India)="" 
Phase of Trial   N/A 
Date of First Enrollment (India)   06/05/2015 
Date of Study Completion (India) Date Missing 
Date of First Enrollment (Global)  Date Missing 
Date of Study Completion (Global) Date Missing 
Estimated Duration of Trial   Years="0"
Months="6"
Days="0" 
Recruitment Status of Trial (Global)
Modification(s)  
Not Applicable 
Recruitment Status of Trial (India)  Completed 
Publication Details   NA 
Individual Participant Data (IPD) Sharing Statement

Will individual participant data (IPD) be shared publicly (including data dictionaries)?  

Brief Summary   To compare and evaluate the bioequivalence study of Methotrexate Tablets 2.5 mg (manufactured for Actavis LLC) with Methotrexate Tablets USP 2.5 mg (DAVA Pharmaceuticals, Inc.,Fort Lee, NJ 07024 USA) in patients with mild to severe psoriasis or rheumatoid arthritis (RA), who are already on established regimens of 2.5 mg every 12 hours under fasting condition. Total expected duration of the study will be of at least 8 days from the day of admission of the first period till the end of study sample collection in period II. There will be at least 7 days duration between the IMP administrations in two study periods. After IMP administration in each period (on day 1), patients will be advised to receive their scheduled dose of 2.5 mg Methotrexate tablet 12 hours apart for additional 2 doses using locally approved drug. A total of 22 PK samples will be collected during each period.
 
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