Irritable bowel syndrome (IBS) is a functional gastrointestinal disorder characterized by a group of symptoms that commonly include abdominal pain, abdominal bloating, associated with altered bowel habits. The exact cause of IBS is unknown, research suggests that the gut microbiome plays a significant role. A healthy gut microbiome is diverse and balanced. In people with IBS, the gut microbiome is found to be less diverse. This imbalance, called gut dysbiosis, contributes to IBS symptoms. Though it is not possible to find an exact correlation of IBS in AÌ„yurveda, some of the disorders such as VaÌ„taja and Kaphaja AtisaÌ„ra, Sangraha GrahanÌ£iÌ„, PravaÌ„hikaÌ„, Pakvashayagata vaÌ„ta have similarity with IBS. The treatment principle in AÌ„yurveda consists of DiÌ„pana, PaÌ„cana measures initially, followed by GraÌ„hiÌ„ and Stambhana measures by means of Vasti karma, ŚamanaosÌ£adhi, PathyaÌ„pathya etc. There is a reference of SaṅgraÌ„hi Vasti in SuśrutasamÌ£hitaÌ„ and AÌ„caÌ„rya Dalhana in his commentary suggests to add Takra as AÌ„vaÌ„pa dravya in SaṅgraÌ„hi Vasti. It has been reported a study that both SaṅgraÌ„hi vasti and Takravasti done as KaÌ„lavasti were found to be equally effective in the management of IBS. But whether it can correct the underlying pathophysiology, especially the gut microbiome is not studied yet. Hence a study is planned to know which vasti is better with respect to gut microbiome i.e Takravasti that contains takra where takra is a natural probiotic that replenishes the intestinal flora or SaṅgraÌ„hi vasti that does not contain takra. 8 participants satisfying diagnostic and inclusion criteria will be taken for study. Extracted DNA samples from the stool will be subjected to 16S rRNA sequencing to know the modulations in gut microbiome. Assessments will be done before the first day of vasti and the next day after last vasti. Data will be collected and will be analysed using relevant statistical tests. The outcome will be submitted in the form of dissertation.