- Prolotherapy is one of the  regenerative medicine treatment and  is an injection based  medical therapy for chronic musculoskeletal pain.

-It has been used for approximately 100 years;however,its modern application can be trace to the 1950s when the prolotherapy injection protocols were formalised by Dr George Hackett, a general surgeon in the united states,remembered as grandfather of prolotherapy.

-prolotherapy ,which stimulates the healing of loosened ligaments and tendons,is a cost effective and safe treatment modality for chronic musculoskeletal pain.

-The prolotherapy technique we use is called as comprehensive prolotherapy or HACKETT-HEMWALL-HAUSER (H3) Prolotherapy.

-A combination of dextrose solution and lidocain is usually used. The concentration of dextrose and lidocaine used in the clinical field are very high (Dextrose-10%to25% ,lidocaine -0.5% to 1%)

-Prolotherapy is the technique of injecting sterile irritants which stimulates physiological acute inflammatory cascade following promotion and Healing of damaged& repairable tissue.

- Many passionate therapists call it a panacea for all ailments It is said to work by raising growth factor levels and increasing cellular proliferation, promoting healing in damaged ligaments. Proponents attribute all articular and periarticular pains to lax, injured or unhealthy ligaments. Prolotherapy can be used at any stage of the disease, and is also applicable to patients who are unwilling or unfit for surgery.

-Prolotherapy has been used in the U.S. for musculoskeletal pain since the 1930’s and is endorsed by numerous main stream practitioners.

-It has been reported in 2020 that over 2500,000 Americans had undergone prolotherapy and that many of the patients reporting benefits from prolotherapy were physicians themselves. Yet, many main stream medical practitioners have still not heard of or do not know much about prolotherapy.

-In Europe and asian countries, many millions of patients receive prolotherapy regularly and have been immensely benefited by the same.

 PROLOTHERAPY AGENT-1. Dextrose 10% -25%

2. Lidocaine 0.5% - 1% without preservatives

·        This analytical Hospital-based study will be carried out at  Gandhi Medical College and Associated Hospital, Bhopal. This study will be conducted on all patients of American Society of Anaesthesiologist physical status I and II aged between 45-75 years

·        Written and informed consent, before participating in the study will be taken from all patients/attendants.

·        METHOD-In this study half of patients will be given 10%dextrose and other half 25% dextrose with fix dose 1% of lodocaine .

-3Injections will be given with interval of one week then will be followed up in 1month 3month& 6month.

-         Site of injection will be intraarticular .

-         8 ml dextrose with 2 ml lodocaine,2.5 -3 ml will be given at multiple site for injured tendon/ligament of osteoarthritis  knee joint.

Assessment will be done by  relieve in pain,range in mobility of joint ,distance of walking.