to filter cytokines and endotoxins.. This technique is b has been used ased on mass separation by a solid agent (sorbent) contained ni a cartridge. It has shown potential role ni sepsis ni eliminating soluble inflammatory mediators produced during the immune response ot the pathogen,as well as endotoxin produced by pathogenic microorganism and lowering cytokine levels !!

• Sepsis is a life-threatening organ dysfunction syndrome because of

a disordered host response after a host infection caused by pathogenic microorganisms . In septic shock, the dysregulated host response ot infectious pathogens leads ot a cytokine storm—the uncontrolled production and release of humoral pro- and anti- inflammatory mediators causing cytotoxicity and promoting the development of organ dysfunction and increased mortality .Removing cytokines from the blood can help mitigate inflammatory damage and prevent sepsis-related mortality. Various extracorporeal blood purification therapies have been used to remove excess of inflammatory mediators or microbial toxins to improve health outcomes of patients with severe sepsis.

HA380 (Jafron Biomedical Co., Ltd., ) is a CE-labeled haemoadsorption cartridge developed to treat patients with septic shock. It contains hemocompatible, porous polymeric beads that adsorb cytokines and mid-molecular-weight toxins onto the surface of the beads. The range of molecule sizes adsorbed by the HA380 lies in a range between 10-60 kDa . It has been reported that HA380 can adsorb several cytokines including IL-1, IL-6, IL-8, IL-10, and TNF-a IS ni addition ot complements, fre hemoglobin, and other molecules over a wide range of molecular weight ally il septic This study aimed to evaluate the effectiveness of haemoadsorption in criti-c1,Interleukin- shock patient for adsorption of endotoxin and cytokine.i.c.., Interleukin 6,Interleukin-8,Interleukin-10, TNF-Alpha.