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CTRI Number  CTRI/2024/09/073172 [Registered on: 02/09/2024] Trial Registered Prospectively
Last Modified On: 26/08/2024
Post Graduate Thesis  Yes 
Type of Trial  Interventional 
Type of Study   Drug 
Study Design  Randomized, Parallel Group, Active Controlled Trial 
Public Title of Study   A study to evaluate the effect of standard dose vs monthly low dose of Rituximab in membranous nephropathy in reducing proteinuria and kidney function. 
Scientific Title of Study   Comparative Efficacy and Safety of Low-Dose versus Standard-Dose Rituximab in Patients with Primary Membranous Nephropathy: An Open-Label Randomized Controlled Trial 
Trial Acronym  NIL 
Secondary IDs if Any  
Secondary ID  Identifier 
NIL  NIL 
 
Details of Principal Investigator or overall Trial Coordinator (multi-center study)  
Name  Sree Bhushan Raju 
Designation  Sr. Professor 
Affiliation  Nizams Institute of Medical Sciences 
Address  Dept of Nephrology Nizams Institute of Medical Sciences Hyderabad Telangana

Hyderabad
TELANGANA
500082
India 
Phone  9030292929  
Fax    
Email  sreebhushan@hotmail.com  
 
Details of Contact Person
Scientific Query
 
Name  Haard Barot 
Designation  Sr. Resident 
Affiliation  Nizams Institute of Medical Sciences 
Address  Dept of Nephrology Nizams Institute of Medical Sciences Hyderabad Telangana

Hyderabad
TELANGANA
500082
India 
Phone  9974704859  
Fax    
Email  haardbarot@gmail.com  
 
Details of Contact Person
Public Query
 
Name  Sree Bhushan Raju 
Designation  Sr. Professor 
Affiliation  Nizams Institute of Medical Sciences 
Address  Dept of Nephrology Nizams Institute of Medical Sciences Hyderabad Telangana

Hyderabad
TELANGANA
500082
India 
Phone  9030292929  
Fax    
Email  sreebhushan@hotmail.com  
 
Source of Monetary or Material Support  
None 
 
Primary Sponsor  
Name  Nizams Institute of Medical Sciences 
Address  Nizams institute of medical sciences, Panjagutta, Hyderabd, Telangana, 500082 
Type of Sponsor  Research institution and hospital 
 
Details of Secondary Sponsor  
Name  Address 
NIL  NIL 
 
Countries of Recruitment     India  
Sites of Study  
No of Sites = 1  
Name of Principal Investigator  Name of Site  Site Address  Phone/Fax/Email 
Dr Sree Bhushan Raju  Nizams Institute of Medical Sciences  Dept of Nephrology Panjagutta Hyderabad
Hyderabad
TELANGANA 
9030292929

sreebhushan@hotmail.com 
 
Details of Ethics Committee  
No of Ethics Committees= 1  
Name of Committee  Approval Status 
NIMS INSTITUIONAL ETHICS COMMITTEE  Approved 
 
Regulatory Clearance Status from DCGI  
Status 
Not Applicable 
 
Health Condition / Problems Studied  
Health Type  Condition 
Patients  (1) ICD-10 Condition: N042||Nephrotic syndrome with diffuse membranous glomerulonephritis,  
 
Intervention / Comparator Agent  
Type  Name  Details 
Comparator Agent  injection rituximab   1 gm IV infusion , 2 weeks apart ( standard dose )  
Intervention  Injection rituximab in low dose 100mg monthly  100 mg In infusion monthly for 6 months till the response  
 
Inclusion Criteria  
Age From  18.00 Year(s)
Age To  65.00 Year(s)
Gender  Both 
Details  Biopsy proven Primary MN (with serum PL2AR positive)
Anti - PL2AR levels more than fifteen and less than hundred and fifty
No clinical and laboratory response to ACE/ARB therapy.
KDIGO defined Moderate and Severe Primary MN
CKD stages one two and three
Nephrotic syndrome

 
 
ExclusionCriteria 
Details  Secondary cause of Membranous nephropathy
Absence of Nephrotic syndrome
KDIGO defined very severe PMN and life-threatening nephrotic complications
Anti - PL2AR levels negative
CKD STAGE four and five
Pregnant females
Concurrent use of any immunosuppression 3 months prior to study
Lost to follow up
Negative consent for the study
Contraindications of immunosuppressive therapy (HIV, Hep B, Hep C)
 
 
Method of Generating Random Sequence   Permuted block randomization, fixed 
Method of Concealment   On-site computer system 
Blinding/Masking   Open Label 
Primary Outcome  
Outcome  TimePoints 
Reduction in proteinuria
complete and partial remission
change in eGFR from baseline 
follow up till 1 year after end of intervention 
 
Secondary Outcome  
Outcome  TimePoints 
to see safety and efficacy of rituximab
change in the anti-PLA2R levels
 
follow up over 1 year after end of intervention 
 
Target Sample Size   Total Sample Size="40"
Sample Size from India="40" 
Final Enrollment numbers achieved (Total)= "Applicable only for Completed/Terminated trials"
Final Enrollment numbers achieved (India)="Applicable only for Completed/Terminated trials" 
Phase of Trial   N/A 
Date of First Enrollment (India)   09/09/2024 
Date of Study Completion (India) Applicable only for Completed/Terminated trials 
Date of First Enrollment (Global)  Date Missing 
Date of Study Completion (Global) Applicable only for Completed/Terminated trials 
Estimated Duration of Trial   Years="2"
Months="0"
Days="0" 
Recruitment Status of Trial (Global)   Not Applicable 
Recruitment Status of Trial (India)  Open to Recruitment 
Publication Details   N/A 
Individual Participant Data (IPD) Sharing Statement

Will individual participant data (IPD) be shared publicly (including data dictionaries)?  

Response - YES
  1. What data in particular will be shared?
    Response - All of the individual participant data collected during the trial, after de-identification.

  2. What additional supporting information will be shared?
    Response -  Study Protocol
    Response -  Statistical Analysis Plan
    Response - Informed Consent Form
    Response - Clinical Study Report
    Response -  Analytic Code

  3. Who will be able to view these files?
    Response - Researchers whose proposed use of the data has been approved by an independent review committee identified for this purpose.

  4. For what types of analyses will this data be available?
    Response - For individual participant data meta-analysis.

  5. By what mechanism will data be made available?
    Response - Proposals should be directed to [sreebhushan@hotmail.com].

  6. For how long will this data be available start date provided 28-11-2024 and end date provided 28-11-2029?
    Response - Beginning 9 months and ending 36 months following article publication.

  7. Any URL or additional information regarding plan/policy for sharing IPD? 
    Additional Information - NIL
Brief Summary  

After getting all necessary clearances, patients will be enrolled in the study as per the inclusion and exclusion criteria. Patients will be randomized using block randomization (with block size of four using Microsoft Excel 2021) into two groups study group and standard arm group. Study group will be receiving low dose monthly rituximab therapy (100mg IV Rituximab) whereas control group would receive standard dose therapy with 1gm Rituximab 2 weeks apart.

All patients will be screened for HIV/HBV/HCV AND KOCH before enrolling in the study. 6 months INH prophylaxis will be given for latent tuberculosis patients prior to treatment with rituximab.

Complete and partial remission to be defined as per KDIGO guidelines.

Patients in study group will be given 100mg iv Rituximab for 3 consecutive months and assessed for complete or partial remission and trends of anti-PLA2R antibody levels.

Patients who attained complete remission or anti-PLA2R antibody titres negative at 3 months will be followed up with conservative therapy upto 12 months.

Patients who achieved partial remission or no remission or persistent positive anti-PLA2R antibody titres at end of 3 months will be further continued monthly (100mg iv Rituximab) dose for further 3 months

Patients who achieved complete remission or anti-PLA2R antibody titres negative at 6 months will be followed up with conservative therapy upto 12 months (from the start of therapy).

Patients who achieved partial remission or decrease in anti-PLA2R antibody titres at end of 6 months will be further given 3 months 100mg Rituximab therapy.

Patients who did not achieve complete remission at end of 6 months will be defined as treatment failure and converted to standard dose of therapy or alternate therapy.

Patients who achieved partial remission or anti-PLA2R antibody titres negative at 6 months will be followed up with conservative therapy for next 12 months (from the start of therapy).

Response rates will be assessed at 3,6, 9 and 12 months. Patient will be monitored 3,6,9 and 12 monthly with CD19/20 levels. CD19/20 levels will be monitored 3 monthly until target levels reached (<1% or < 5cells).

All patients will be undergoing 3 monthly assessments for proteinuria (Complete urine examination/Urine spot PCR/S. total protein/S.albumin/24 hours UP) and renal function (s. creat and urea levels).

Anti-PLA2R antibody levels will be done at baseline,3,6, and 9months and trends will be analyzed.

 

Patients not achieving complete and partial remission will be termed as non-responders. Relapse will be defined as recurrence of proteinuria >3.5gm after achieving complete and partial remission.

 

Conservative therapy will also be given simultaneously to patients, ACE/ARBs at maximum tolerable doses. 

The primary outcome is the remission rate (complete remission + partial remission) at end of three, six, nine and twelve months.

The changes in Anti-PLA2R levels at end of three, six, nine and twelve months, changes in proteinuria levels, changes in eGFR at end of three, six, nine and twelve months will also be considered in primary outcomes.

Infections requiring hospitalization, serious allergic reactions, malignancies, development of Koch or any severe septicemia will be considered as serious side effects and assessed.

 
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