Will individual participant data (IPD) be shared publicly (including data dictionaries)?  

Response - NO

Each tooth will be anesthetized and isolated with a rubber dam followed by caries removal. The access cavity will be prepared using a high-speed endo access bur. The working length (WL) will be determined radiographically using RVG by placing a size #10 K-file. The patients will be randomly divided into three groups: Group I: 2% chlorhexidine gluconate (CHX), Group II: Neem juice extract (Azadirachta indica), Group III: moringa juice extract (Moringa olifera).Pretreatment sample (S1) will be obtained by using a sterile paper point immersed in transport media first followed by placing it into the canal for 60secs and then transferred to the test tubes containing transport media (Peptone water). Two samples will be taken for each tooth. Biomechanical preparation (BMP) will be done using step-back technique up to master apical size #40 K-file. Irrigation will be done with 6 mL of the irrigants. Post-instrumentation sample (S2) will be similarly collected after irrigation as S1. It will be sent to the laboratory for processing within 2 hours. After this, teeth will be obturated using gutta-percha as a master cone and ZOE sealer. Microbiological samples (S1, S2,) will be sent to the lab Tropical Institute of Ecological Sciences(TIES)at Kottayam. Samples will be preincubated for 30 minutes and shaken vigorously in a vortex mixer for 60 seconds and will be plated on brain heart infusion agar and colonies will be counted after 24 hours using classic bacterial counting method. Both the session of treatment will be performed by the same examiner for each patient. The examiner will be aware of the material being used in every patient.

A fidelity checklist will be developed using pertinent literature and after due consideration. An observer will fill the fidelity check list to ensure objectivity and transparency.